GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS DDIP2PLAS 5852 bp ds-DNA INV 25-MAY-1991
DEFINITION Dictyostelium discoideum Ddp2 plasmid DNA containing gene essential
for replication
ACCESSION X51478
KEYWORDS plasmid; plasmid Ddp2; rep gene.
SOURCE Dictyostelium discoideum DNA.
ORGANISM Dictyostelium discoideum
Eukaryota; Animalia; Protozoa; Sarcomastigophora; Sarcodina;
Rhizopoda; Eumycetozoa; Dictyostelia; Dictyosteliida;
Dictyosteliidae.
REFERENCE 1 (bases 1 to 5852)
AUTHORS Slade,M.B.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 5852)
AUTHORS Slade,M.B., Chang,A.C. and Williams,K.L.
TITLE The sequence and organisation of Ddp2, a high copy number plasmid
of Dictyostelium discoideum
JOURNAL Plasmid 24, 195-207 (1990)
STANDARD full automatic
REFERENCE 3 (bases 1 to 5852)
AUTHORS Chang,A.C., Slade,M.B. and Williams,K.L.
TITLE Identification of the origin of replication of the eukaryote
Dictyostelium discoideum nuclear plasmid Ddp2
JOURNAL Plasmid 24, 208-217 (1990)
STANDARD full automatic
COMMENT *source: strain=WS380B; cell line=HU2362;
From EMBL 26 entry DDP2PLAS; dated 15-APR-1991.
FEATURES Location/Qualifiers
repeat_region 572..1072
/rpt_type=inverted
misc_feature 915..924
/note="palindromic sequence tgtcatgaca"
misc_feature 938..947
/note="palindromic sequence tgtcatgaca"
misc_feature 1100..1109
/note="palindromic sequence tgtcatgaca"
repeat_region 1137..1638
/rpt_type=inverted
misc_feature 1263..1272
/note="palindromic sequence tgtcatgaca"
misc_feature 1285..1294
/note="palindromic sequence tgtcatgaca"
misc_feature 1643..1652
/note="palindromic sequence tgtcatgaca"
misc_feature 1665..1674
/note="palindromic sequence tgtcatgaca"
misc_feature 2172..2181
/note="palindromic sequence tggcatgaca"
CDS 2378..5041
/note="essential for plasmid replication"
/gene="rep"
/codon_start=2378
BASE COUNT 2296 a 652 c 709 g 2195 t
ORIGIN
1 tcgacaaata tcaagggttg gaatcttgta aaaattttcc cgttatcgca aacaatcaaa
61 gtttaagctt caatcttcaa taataatttt aactttatct ctttcaattt taataatttt
121 tttcaaaaat tgaaaatggt atagatcgat agatcacctt ttttagagat aaaccatgaa
181 aaagacataa aaaataaagg tcatcaaagt attaaaaaaa attaattatc tttttaactt
241 tgaaaaaaaa aaataaaaaa aaataaaaaa aaaaaattct ttgttttaat aacttttaaa
301 attattaaaa atagtataga tttaaagatc acaatttttt ataattaact acataaaatt
361 tataaaaaat gagggtcatg aagatatata aataattatt taattattaa atatttaatt
421 atttatttaa cttaaaaaaa aaaaaaagga aaaaaaggaa aaaaaaagtg aaaaaggtgg
481 gaaaatgaaa aaaaaagtga aaaaaatgcc caaaaaaatt tttatatgag aaaaaaatta
541 cgtaaaaaaa aaataagtct gacccaaatc gaaaaataat aaaagagggg aaagtaatta
601 taactaggtt agttttttat aatttttaca tatttgttaa taacttttaa ttttgaatca
661 tatgatatta catcgtcccg ttgaaaaaaa aaaaaaaaat ttttttttca aacattttca
721 ttttttaaaa aatgatataa aattttaaac taaactattt tattaaatac aaatatataa
781 ctttatctta atcaattttt ttggtttata catatttatg ttcgtactga agtatagatc
841 ttattactaa agtttcaaaa gttttaaaaa aaattaaagg gggtaaatat ataactttct
901 gtttttttca attctgtcat gacagaaagg taaaaagtgt catgacaaaa aaaaaaaaaa
961 aaaaaattta tttcttcaat aggtattgaa atgacctccg tttttaataa aaagtatata
1021 tttgtgcttt cctagatgaa ataaggttat ttgagcttaa ttcagattat tataagatta
1081 ttataaaaaa atgaaaaact gtcatgacag tttttgtaag tttcttatag ttttttttaa
1141 tgatctgaat taagcttaaa taaccttatt tcatctagac gagcacaaat atatactttt
1201 tattaaaaac ggaggtcatt tcaataccta ttgaagaaat aaattttttt tttttttttt
1261 tttgtcatga cacttttttt tttttgtcat gacagaattg aaaaaaacag aaagttatat
1321 atttaccccc tttaattttt tttaaaactt ttgaaacttt agtaataaga tctatacttc
1381 agtacgaaca taaatatgta taaaccaaaa aaattgatta agataaagtt atatgtttgt
1441 atttaataaa atagtttagt ttaaaatttt atatcatttt ttaaaaaatg aaaatgtttg
1501 aaaaaaaaaa tttttttttt ttttttcaac gggacgatgt aatatcatat gattcaaaat
1561 taaaagttat taacaaatat gtaaaaatta taaaaaacta acctagttat aattactttc
1621 ccctcttttt tttttttttt tttgtcatga cacttttttt tttttgtcat gacacttttt
1681 ttttaaaaaa aaaaaaaaaa atgttaaaat actatttgat gacattcatt tttcctagtt
1741 tttttttaga tagatataaa aataaattgc ctatcgatat atacttaatt tattaagatt
1801 gaataatatt ttaattttta ataaattcta cttttttttt ttttttcttt tttttttaaa
1861 ttttaaaatt tttttttttt attagatctc ataattaaaa atcaatttaa aattaaaagt
1921 tatttttaaa tatgcaaaaa ctataaaaaa ctaatgtagt ttaaccaact tttttctatt
1981 tctttttttt tttttttttt ttttttactt tgaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2041 aaaccctcat tataaatatt aattactttg gttttttttg attttttttt taataaattt
2101 aaaattttat tctctatcta attatacctt atttataaat attggataat atatcaaata
2161 tttatcagtt ttggcatgac aattttaatt atatttattt tttgattaat tttttttttt
2221 tttttttttt aaaatttctt tttttttttt tttattttta atttttaatt tttatttttc
2281 ccacactttc attttatttt attttattta ttgtaaattc attttattta tttttaatta
2341 aatagttttg gtttaatttt attcaaagat tttaaaaatg gacgaactta tttcttggga
2401 taggtttttt aagttttttg taatactttt ggaagaattc aaaggttgta aaagaaatga
2461 tgtgcgtttg agtgtcgatt atgacattct ttctggtatt tattcgccac gtacatttgt
2521 actaaaggaa gtctttagag cagtggccgt ctcttatgat gaatctgaaa tagatttatt
2581 cagattgggt tcagtgtttc ctggtacttc tttatattca tatattccag gtattttcag
2641 tttaaaagat ttccttttaa tttcaaaaac taaatcgggt aaaataagag tttcggatgt
2701 agatcaagca atattaattt ttgatcattt ttctagaatt tcagataaac aagtatttcg
2761 taaagatatt attccaggtt atagaacctt tgaaaaatca atatcgagcg agtacaaaat
2821 ctcggatggt cgtgctgcag gagtgagttg gttcaattta gttagtaaaa taagcactta
2881 ttgtaaaaat catcccttgt ttgccgaaaa tccaacatat aaacatgtgg attttatatc
2941 aatgttatca ctggtgcatg gaatcattgt tgattcccaa aatgaagatg agaataatgt
3001 ttcggcaatg tactctctga atccttttgt ggatcttgaa aaaagtgata taccaggggc
3061 tgttcaaagt agagttacta caaatagaac tagaggttca aggtctaatt ccaatttgaa
3121 taatccaaca acaacaacaa ctactactac cactactaca actaccgcac caattactac
3181 tagaagtaaa agaaaatctg acgactctgt acaagaacaa agctcacgac aaccaaaaac
3241 ctcgagaaag tctggttctc ttaaggatgt cagaattaac aatatatcag tagattcaag
3301 ttccagtgaa tctgatgtga ttatgtcagt ttcaaaccgt ttaaaatgtt atcttttgga
3361 agcagttgta aacaaaggag agatcggttt agaagtcgtc aaagaagttt taaaagattt
3421 acaggacaaa aattatgcca caggtttact tgaaaacatt ttcaatcaca acaagtctga
3481 aagggtcata acactttcaa gtagtttttt tgaaattgct tcaaaaatta actatgatga
3541 agttaagttc agtgaactca gtattgatgt tctggaatcg gcaaagagat taacattcga
3601 gaaaaataca aatatattaa ttccaaccaa taattttaaa gaaggttttg aatttttatg
3661 ggttccaatt gttaatggta ttgcttcaac ttctgtcttt gtttcaccaa ataattattc
3721 aagtggttca tttgcaaatg tagaatctgc tttaaagttg attcatcttt gcatttcttt
3781 aggaaatata aatggtttcc tctctattag atcaattaca tttgatacat ttaaatcgat
3841 tacaaaggat cttattccaa tgtcgaaaag aatgctggac cttgaacaag gcttccgaaa
3901 acttagagat gcttggaata atagtaataa aaaatccaaa gttcaagata gtgatattag
3961 tggcatcgat acagaggata caaagttgat atcatttgtc cacgagttta taaatgataa
4021 tttatattta aaactatcaa aagaagaaga tggactaatg ctagtagact ttccaacatc
4081 aacacttttt atgagataca atccaaatag cattgataac aaagttggtt tcatgttcca
4141 ttgccgttca gagatttcaa agtttcaaag ttgtaaaaac cactcgatag ataaccttgt
4201 tttatcattt actccaaata acattaaaaa tatatcacag gataatgaaa atgagcttaa
4261 aaagaaatat tcgttgatgg tcagtgattt tagaaatgtt ccaaaggtga caccaaaatt
4321 tataccttct gaatttaaaa ggtttacaat cattacgttc acaaacaatt catacaatgc
4381 caatagagta tttgcgtttg acgacatctc aagtggaatt tcaatcacaa atgttaaaaa
4441 tatccacgca aagggtcaac gaaactttga aatctacgaa acattactgg gaagtaccag
4501 gattattcgt gcatttttct gcgctccatg cttgatccaa atcaataatt ttaaatttgc
4561 cacagataag ttaattgatg accaaagtgt aaatcaccag attgcatctt tggaaattaa
4621 aaacttatca tatcttccgc tcgacatcaa ggttagaggt agtacagttg gaacgattaa
4681 gggtggagag acagctccta ttattataaa ctcagaagaa tttacgtttt ctatctcatg
4741 ccttgatatt agatttagtg catccttaat ttctaaaaca aaactaagcc aacttccaac
4801 atttgctcca gatgaaaggt acaataaaga gactaacatt ttaaaagttt tggatcaatg
4861 tgatgaactt actcgaacgt ttttaaataa ctataaaata gctaataaac tatcaaccat
4921 tgaaaattat ttatataata attttatggg actagaagat gaagatgaag atgaagatga
4981 agatgaagat gaagatgaag atgaagatga agatgaagat gaagacgaag atgggtattg
5041 aattatcata ctttaaaaat taattaaata aataaaaaaa aaaaaatgat ttcaatttaa
5101 atatatacat atatatatat ataaaatgag attaataaaa cttttgagac caacatttaa
5161 tgagatttct gatgctgttt attttgcctg gaatgagagc aaaaggctaa aaaacatgag
5221 agagaatata ataataaagg aaaacttggg aaaaaggatc tagtatccat ttccatatta
5281 atccgtgcag tactattaat taaaaaaata ctttaaaaaa aattttaaaa acatggaaaa
5341 ttatatagat cgatagatca ctaattttta aaattaaata tattaaattt ataaaaattg
5401 aagttcatca agatatatag ataattattt aattatttga atttttaaaa aaaaaaaaaa
5461 aaaaaaaaaa aaaatcaaat atgtttattg ttttaagatt ttttaatctc gtcaatgatt
5521 ttaaaataaa aatcgataca taattttaaa aaaaaccctt tacatttttt attttaattc
5581 caaatttata cattttttat tttttttttt tttttttttt ttttttttaa tttaaatttt
5641 tttttttttt ttttttttat ttatttaaaa tttaattatt aattttataa ataaaaaata
5701 gaaatataag taaaaaaaca aacaacaaat aacatatata aaaaaataca aataacaaat
5761 aattaaataa attaaataac cattaaaaat gtatattaat aaatttaaaa gatctttatt
5821 agtactattg ttactttgta atattcttcc tg
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