GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS ECOIS1A 902 bp ds-DNA BCT 25-MAY-1991
DEFINITION Escherichia coli DNA for insertion sequence is1A
ACCESSION X52534
KEYWORDS insA gene; insertion sequence; transposon.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 902)
AUTHORS Umeda,M.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Umeda,M. and Ohtsubo,E.
TITLE Four Types of IS1 with differences in nucleotide sequence reside in
Escherichia coli K-12 chromosome
JOURNAL Gene 98, 1-5 (1991)
STANDARD full staff_review
COMMENT SWISS-PROT; P03827; ISA1$ECOLI.
*source: strain=K12, W3110; library=lambda EMBL4; clone=6H3; **map:
map position=0.4; See <X04382> for overlapping sequence.
From EMBL 26 entry ECIS1A; dated 17-APR-1991.
FEATURES Location/Qualifiers
insertion_seq 61..828
/note="is1A insertion sequence"
repeat_unit 61..77
/note="inverted repeat A"
misc_feature complement(76..773)
/note="insA-B'-insB frame"
CDS complement(501..773)
/note="insA peptide (AA 1-91)"
/codon_start=773
repeat_unit 812..828
/note="inverted repeat A'"
BASE COUNT 221 a 233 c 225 g 223 t
ORIGIN
1 aataaataga ggaatctgat tacttccttc atggggatgc tgaaaagagt agtaattgct
61 ggtaatgact ccaacttatt gatagtgttt tatgttcaga taatgcccga tgactttgtc
121 atgcagctcc accgattttg agaacgacag cgacttccgt cccagccgtg ccaggtgctg
181 cctcagattc aggttatgcc gctcaattcg ctgcgtatat cgcttgctga ttacgtgcag
241 ctttcccttc aggcgggatt catacagcgg ccagccatcc gtcatccata tcaccacgtc
301 aaagggtgac agcaggctca taagacgccc cagcgtcgcc atagtgcgtt caccgaatac
361 gtgcgcaaca accgtcttcc ggagactgtc atacgcgtaa aacagccagc gctggcgcga
421 tttagccccg acatagcccc actgttcgtc catttccgcg cagacgatga cgtcactgcc
481 cggctgtatg cgcgaggtta ccgactgcgg cctgagtttt ttaagtgacg taaaatcgtg
541 ttgaggccaa cgcccataat gcgggctgtt gcccggcatc caacgccatt catggccata
601 tcaatgattt tctggtgcgt accgggttga gaagcggtgt aagtgaactg cagttgccat
661 gttttacggc agtgagagca gagatagcgc tgatgtccgg cggtgctttt gccgttacgc
721 accaccccgt cagtagctga acaggaggga cagctgatag aaacagaagc cactggagca
781 cctcaaaaac accatcatac actaaatcag taagttggca gcatcaccta cctcaatgtg
841 tatcacaata tccatattct ttgtggggga gtctggagat tgagtagata ttcttgttca
901 ga
//