GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS ECOIS1F 881 bp ds-DNA BCT 25-MAY-1991
DEFINITION Escherichia coli DNA for insertion sequence is1F
ACCESSION X52538
KEYWORDS insA gene; insertion sequence; transposon.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 881; 35 to 842)
AUTHORS Umeda,M.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 35 to 842)
AUTHORS Umeda,M. and Ohtsubo,E.
TITLE Four Types of IS1 with differences in nucleotide sequence reside in
Escherichia coli K-12 chromosome
JOURNAL Gene 98, 1-5 (1991)
STANDARD full automatic
COMMENT SWISS-PROT; P19767; ISA2$ECOLI.
*source: strain=K12, W3110; library=lambda EMBL4; clone=9H9; **map:
map position=97.5;
From EMBL 26 entry ECIS1F; dated 17-APR-1991.
FEATURES Location/Qualifiers
insertion_seq 55..822
/note="insertion sequence is1F"
repeat_unit 55..71
/note="inverted repeat A"
misc_feature 110..807
/note="insA-B'-insB frame"
CDS 110..382
/note="insA peptide (AA 1-91)"
/codon_start=110
mutation 595..597
/note="amber mutation"
repeat_unit 806..822
/note="inverted repeat A'"
BASE COUNT 210 a 209 c 231 g 231 t
ORIGIN
1 atgatgtggt ctgctattat tgacatcctc actgccctaa aggatgggga tttcggtaat
61 gctgccaact tactgattta gtgtatgatg gtgattttaa ggtgcttgcg tggcttccat
121 ttccatcaga tgtccttcct gctccgctac tgaaggcgtg gtgcgtaacg gcaaaagcac
181 tgccggacat cagcgctatc tctgctctcc ttgccgtaaa acatggcaac tacagttcac
241 ttacaccgcc tctcagcccg gtaagcacca gaaaatcatt gatatggcca tgaatggcgt
301 cggatgtcgc gccagtgcac gcattatggg cgttggcctc aacacggttt tacgtcactt
361 aaaaaactca ggccgcagtc ggtaacctcg cgcatacaac cgggcagtga tgtgattgtc
421 tgcgctgaaa tggacgaaca gtggggctac gtcggtgcta aatcacgtca gcgctggctg
481 ttttacgcgt atgacaggat acggaggacg gttgtggcgc acgtcttcgg tgaacgcact
541 ctggccacac tggagcgtct tctgagcctg ctgtcggcct ttgaggtcgt ggtatagatg
601 acggatggct ggccgctgta tgaatcacgc ctgaagggaa agctgcacgt tatcagcaag
661 cgttacactc agcgcattga gcgacataat ctgaatctga gacaacatct ggcaaggctg
721 ggacggaagt tactgtcgtt ctcaaaatcg gtggagttgc atgacaaggt catcgggcat
781 tatctgaaca taaaacacta tcagtaagtt ggagtcatta ccgaccatgt ttatttcata
841 cattgtgggt attgttctta ttatcgccgc taatcaataa a
//