GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS BACPOLC1 4866 bp ds-DNA BCT 25-MAY-1991
DEFINITION Bacillus subtilis polC gene for DNA polymerase III
ACCESSION X52116
KEYWORDS DNA polymerase; DNA polymerase III; polC gene; polymerase.
SOURCE Bacillus subtilis DNA.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 4866)
AUTHORS Brown,N.C.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 4866)
AUTHORS Hammond,R.A., Barnes,M.H., Mack,S.L., Mitchener,J.A. and Brown,N.C.
TITLE Bacillus subtilis DNA polymerase III: complete sequence,
overexpression, and characterization of the polC gene
JOURNAL Gene 98, 29-36 (1991)
STANDARD full automatic
COMMENT *source: strain=BD541; **map: map position=145 degrees; See
<M22996> for overlapping sequence.
Data kindly reviewed (04-JUL-1990) by Hammond R.A.
From EMBL 26 entry BSPOLC1; dated 17-APR-1991.
FEATURES Location/Qualifiers
RBS 27..34
/note="pot. ribosome binding site"
CDS 46..4356
/note="DNA polymerase III (AA 1-1437)"
/codon_start=46
BASE COUNT 1499 a 930 c 1141 g 1296 t
ORIGIN
1 ggtaccgctt cacttatgat gtttttaggg agggatactg tcttaatgga acagttatca
61 gtaaacagaa ggcagtttca aattcttctg cagcagatta atatgacaga tgataccttc
121 atgacatact ttgaacatgg cgagattaaa aagctgacaa ttcacaaagc ttctaagtct
181 tggcattttc attttcaatt taaatctttg ctgccttttc aaatatatga cacattaaca
241 acgaggctga cgcaatcgtt tgcccacata gcaaaagtga catcttcaat tgaagttcag
301 gatgccgagg tcagtgaaag tatcgttcaa gactactggt cacgctgcat tgaagaactg
361 cagggcattt cgccgccgat tatcagtctt ttaaaccagc aaaaaccgaa gctgaagggc
421 aataaactga ttgtcaaaac caaaacagat acagaagcgg ctgcgctaaa gaacaaatac
481 agttccatga ttcaagcaga ataccgtcaa tttggctttc cggatcttca gcttgatgct
541 gaaatatttg tatccgagca agaagttcaa aagtttcggg agcaaaagct tgcggaagac
601 caagagcggg ctatgcaggc cttgattgaa atggagaaga aagataaaga aagtgatgaa
661 gaccaagcac catctggtcc tcttgttatc ggttatcaaa ttaaagataa cgaggaaatc
721 cgaacacttg acagcatcat ggacgaagaa cggagaatta cggtccaagg ttatgtgttt
781 gatgtggaga cgcgcgagct gaagagcggg cgcacgctgt gtatcttcaa aattacagac
841 tatacaaata gtattttgat caaaatgttt gcacgtgaaa aagaagatgc ggcgctgatg
901 aagtctctga aaaaaggaat gtgggtaaaa gcacgcggaa gcattcaaaa tgatacattt
961 gtcagagacc ttgtcatgat cgcaaatgac gtaaacgaaa taaaagcaaa aacccgtgaa
1021 gattcagcac ctgaaggaga aaaaagagtg gaattgcatc ttcattcccc aatgagccaa
1081 atggatgctg ttacgggtat cggaaagctt gtcgaacagg cgaaaaaatg ggggcatgag
1141 gccatcgctt tgaccgacca tgctgtcgtt caatccttcc ctgatgcgta ttctgcggcc
1201 aaaaagcatg gaattaaaat gatttacggg atggaagcga atctcgtgga tgatggcgtg
1261 ccaattgctt ataatgccgc acatcgtctg ctcgaagaag aaacatatgt tgtttttgac
1321 gttgagacga caggattgtc tgctgtatac gataccatta ttgagctggc tgcagtaaaa
1381 gtaaaaggcg gagaaattat tgataaattt gaggcgtttg cgaacccgca tcgtccgctt
1441 tccgccacaa tcatagagct gacagggatc acagatgata tgctacaaga cgctccggat
1501 gtcgtagatg taataagaga tttcagagaa tggattggcg atgatattct tgtcgctcat
1561 aatgcaagct ttgatatggg attcttaaat gtagcctata aaaaacttct tgaagtcgaa
1621 aaagctaaaa acccagtcat tgatacgctt gaacttggac gttttctcta tccggaattt
1681 aagaaccacc ggttgaacac actttgtaaa aagtttgata tcgagctcac acagcatcac
1741 cgtgcgatct atgatactga ggcaaccgct tatttgcttc tgaaaatgct gaaagacgca
1801 gctgaaaaag gtattcagta ccatgatgag ttgaatgaaa atatgggtca gtccaatgct
1861 tatcaaagat caagaccgta tcatgcaaca ttacttgccg tgaacagcac gggacttaaa
1921 aatttattta agcttgtgtc actttctcat attcattatt tttacagagt gccgcgtatt
1981 ccgagatctc agcttgagaa atacagggaa gggcttctga tcggttctgc ttgtgacagg
2041 ggagaggttt ttgagggaat gatgcaaaaa tcgcctgaag aggtggaaga tatcgcgtca
2101 ttctatgatt accttgaggt tcagccgcct gaagtgtatc gtcacttgct ggagcttgaa
2161 ctggtccgtg atgaaaaagc gctgaaagaa attattgcga atatcacgaa gctgggggaa
2221 aagcttaata aaccggttgt tgctacggga aatgttcatt acttgaatga tgaggataaa
2281 atctacagaa agattttaat atcctcacaa ggcggggcaa atccgctgaa taggcatgaa
2341 ctgccgaaag tgcatttcag aacgacagac gaaatgcttg aagctttttc tttcttaggt
2401 gaagaaaaag cgaaggagat cgtagtcacc aatacccaaa aggttgcttc tttagttgat
2461 gacatcaagc cgattaaaga tgatttatat acgccgaaaa tcgaaggcgc tgatgaagag
2521 atcagagaaa tgagctatca gcgtgcaaga agcatttacg gggaagagct gcctgaaatt
2581 gtcgaagcgc ggattgaaaa agagttaaag agtattattg gccacggatt tgctgttatt
2641 tacttgatct ctcacaaact tgtaaaacgt tcactagatg acgggtatct cgttggttcc
2701 cgtggttccg taggatcttc attagttgcg acacttactg agattactga ggtaaacccg
2761 ctgccgccgc actatgtttg tcctgagtgc cagcattctg agttctttaa tgacggttct
2821 gtcggttctg gttttgacct gcctgacaag acatgccctc attgcggaac gcctttgaaa
2881 aaagacggcc atgatattcc atttgaaacg ttcttaggat ttaaagggga caaagtacct
2941 gatatcgatt tgaacttctc aggggaatat cagccgcaag cacacaatta cacaaaagta
3001 ttgttcggag aagacaatgt atatcgtgcg ggaacaatag gcacggtggc agaaaaaaca
3061 gcctacggtt atgtaaaagg ctatgccgga gacaacaatc ttcatatgcg cggtgccgaa
3121 atagatcggc tcgtacaggg atgcacaggt gtaaaacgta caactggaca gcaccctggc
3181 ggtattatcg tagttccgga ttatatggat atttatgatt tttcaccgat ccagttcccg
3241 gcagatgcca caggttcaga gtggaaaacg actcattttg atttccactc catccatgac
3301 aacctgttaa aacttgatat tctcggacac gatgacccga ctgttattcg gatgcttcaa
3361 gacttaagcg gaatagatcc gaaaacaatt ccgacggatg atcctgaagt gatgaagatc
3421 ttccagggaa ccgaatcact cggtgtgact gaagaacaga ttggctgtaa aacgggcact
3481 cttggaattc ctgaattcgg aacccgattt gtccggcaga tgcttgaaga tacaaagccg
3541 accacttttt ctgagctcgt tcagatttca ggcttgtctc acggaactga tgtatggctt
3601 ggcaatgcac aggagctcat ccacaataat atttgtgagc tgagtgaggt tatcggctgc
3661 cgtgatgaca ttatggttta tttaatctat caaggccttg agccgtccct tgcctttaaa
3721 atcatggaat tcgtgcgtaa aggaaaagga ttaacgcctg aatgggaaga agaaatgaaa
3781 aataacaatg tcccagactg gtatattgat tcctgtaaaa agattaaata catgttcccg
3841 aaagcccacg ccgcggcata tgtcttaatg gcagtccgca ttgcttactt taaagtgcat
3901 catgctcttt tgtattatgc ggcttatttt accgttcgtg cagatgactt tgatattgat
3961 acaatgatca agggctctac agcaatcaga gcggtaatgg aggatataaa cgctaaagga
4021 cttgatgctt caccgaagga aaagaacctt ctgactgttt tagaattagc gcttgagatg
4081 tgtgagagag gctattcatt ccaaaaagtc gatttatatc gctccagcgc cacagagttt
4141 attattgacg gcaacagtct tattccgccg tttaactcta ttccagggtt agggacgaac
4201 gctgctttga acattgtaaa agctcgcgaa gaaggcgaat tcctctcaaa agaagatttg
4261 caaaagagag ggaaagtatc aaaaacgatt ttagagtact tagatcgcca tggctgtctg
4321 gagtcactgc ctgatcaaaa ccaattgtca ctgttctaat atggaaagca gaatttctca
4381 gaaattctgc ttctatgcat acataagcgc aaaaagtgcc atcgtaatat tagagtttct
4441 gtcacttgct taggtatgaa ggtaagcgta tatccatttg caataaaaat atggttatgg
4501 tatagtttta ttggaaatgc taacgattac cgaggcaaag agtggggaaa cccgctcttt
4561 tgtattgaac aggagaattt tgtctcgaca tgttcatcgt ttacttttta gcccctgctc
4621 ttttgaagca gggtttttat gcagagtgac gagacgaata tgagatcgac agcacaagga
4681 ggaagaacat gagcaaaaaa gtgactgaca ccgttcaaga aatggctcag ccaatcgtag
4741 acagccttca gctggaactc gttgacattg aatttgtcaa agagggccaa agctggttcc
4801 ttcgcgtgtt tattgattcc gatgacggtg tggatattga ggaatgtgcc aaagtaagcg
4861 aagctt
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