GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS ECORRNGTN 1828 bp ds-DNA BCT 25-MAY-1991
DEFINITION E.coli terminator sequence of RNA G operon gene.
ACCESSION X56780 X57090
KEYWORDS 23S-5S spacer region; URF; rrng operon; transposase; wit A gene.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1828)
AUTHORS Squires,C.L.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1828)
AUTHORS Albrechtsen,B., Ross,B.M., Squires,C. and Squires,C.L.
TITLE Transcriptional termination sequence at the end of the Escherichia
coli ribosomal RNA G operon: complex terminators and
antitermination.
JOURNAL Nucleic Acids Res. 19, 1845-1852 (1991)
STANDARD full automatic
COMMENT From EMBL 26 entry ECRRNGTN; dated 18-APR-1991.
FEATURES Location/Qualifiers
prim_transcript complement(<427..>489)
/note="URF with homology to rrnE URF2"
/gene="URF"
prim_transcript complement(<1250..>1828)
/note="transposase (Tn1000)"
prim_transcript <1..521
/partial
/evidence=EXPERIMENTAL
prim_transcript <668..>1225
/note="putative transport protein similar to citB"
/gene="witA"
rRNA 1..132
/partial
/product="23S gene of rrnG operon"
/gene=" 23S"
misc_feature 133..224
/note="23S-5S spacer region contains the 3'processing seq.
of 23S and 5'processing seq. of 5S. The region is highly
conserved in rrn operons."
rRNA 225..344
/product="5S gene of rrnG operon"
/gene=" 5S"
misc_feature 332..351
/note="3'processing stalk of 5S /possible role in
termination."
terminator 349..388
/evidence=EXPERIMENTAL
terminator 385..521
/evidence=EXPERIMENTAL
misc_feature 403..428
/note="function unknown"
CDS complement(427..489)
/note="URF with homology to rrnE URF2"
/gene="URF"
/codon_start=489
mat_peptide complement(430..489)
/note="URF with homology to rrnE URF2"
/gene="URF"
mat_peptide 668..1222
/note="putative transport protein similar to citB"
/gene="witA"
CDS 668..1225
/note="putative transport protein similar to citB"
/gene="witA"
/codon_start=668
misc_feature 1216..>1828
/note="insertion of Tn1000 seems to relieve a growth
liability caused by the elevated copy-number of plasmid
pLC23-30 witA genes"
/product="transposon Tn1000"
CDS complement(1250..>1828)
/codon_start=1828
/product="transposase (Tn1000)"
/codon_start=1828
mat_peptide complement(1253..>1828)
/codon_start=1828
/product="transposase (Tn1000)"
BASE COUNT 457 a 454 c 432 g 485 t
ORIGIN
1 cccgagatga gttctccctg accctttaag ggtcctgaag gaacgttgaa gacgacgacg
61 ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct aaccggtact aatgaaccgt
121 gaggcttaac cttacaacgc cgaagctgtt ttggcggatg agagaagatt ttcagcctga
181 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggccgta
241 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaaccgcgt agcgccgatg
301 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat tatgcgaaag
361 gccatcctga cggatggcct ttttgcattg gcgcagaaaa aaatgcctga tgcgacgctg
421 cgcgtcttat actcccacat atgccagatt cagcaacgga tacggcttcc ccaacttgcc
481 cacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa taaccatttt
541 cctgctaact aaattcatgg ttaaggttgc ataatgatat gcaacaaatg tataatattt
601 cctttacaaa aaaaataaac aaaagcgacc gacaaaagca tcggattacg gcaggagaca
661 taatggcatg gctgaaagta ctgtaacggc agacagcaaa ctgacaagta gtgatactcg
721 tcgccgcatt tgggcgattg tgggggcctc ttcaggtaat ctggtcgagt ggttcgattt
781 ctatgtctac tcgttctgtt cactctactt tgcccacatc ttcttccctt ccgggaacac
841 gacgactcaa ctactacaaa cagcaggtgt ttttgctgcg ggattcctga tgcgcccaat
901 aggcggttgg ctatttggcc gcatagccga taaacatggt cgcaaaaaat cgatgctgtt
961 atcggtgtgt atgatgtgtt tcggatcgct ggttatcgcc tgcctcccag gttatgaaac
1021 tataggtacg tgggctccgg cattattgct tctcgctcgt ttatttcagg gattatctgt
1081 tggcggagaa tatggcacca gcgccaccta tatgagtgaa gttgccgttg aagggcgcaa
1141 aggtttttac gcatcatttc agtatgtgac gttgatcggc ggacaactgc tagccctact
1201 ggttgtcgtg gttttagggg tttgagggcc aatggaacga aaacgtacgt taaggagata
1261 attcgttgtt tatatttaaa tttagagctc tcagttcccc ttttaaaata tcctctggca
1321 acgtgaatgt ataatggccc aacatattga tatgcccgtg catcagggga gatagccgag
1381 cgatatcttc atctataatt tcttcgccat tacggcgcat ccagctcaac gcttcctcca
1441 tatagagcgt gttccacaga accactgcat tagtaaccag gcccagcgcc cccagttgat
1501 cttcctgccc ttcacgataa cgctttctga tctctccgcg ttgtccgtaa caaatcgcac
1561 gagccacagc gtggcggcct tctcctcgat taagctgcgt caggatccgc cgacgataat
1621 cttcatcatc aatataattg aggagatata gcgttttgtt tacacgccct acttccataa
1681 ttgcctgtgc cagtcctgat gggcgcgagc ttttcagtaa agagcgaatg agttctgacg
1741 catgaattgt acccaacttc agcgaaccag cggttcgcat catctcatcc cactgactct
1801 ccgcttttga cagatctgca tatcctcg
//