GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS ECOEBGRB 4265 bp ds-DNA BCT 25-MAY-1991
DEFINITION E. coli ebgR and ebgA genes for ebg repressor and
beta-galactosidase
ACCESSION X03228 M13700 M13796
KEYWORDS beta-galactosidase; galactosidase; repressor.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 984)
AUTHORS Stokes,H.W., Betts,P.W. and Hall,B.G.
TITLE Sequence of the ebgA gene of Escherichia coli: Comparison with the
lacZ gene
JOURNAL Mol. Biol. Evol. 2, 469-477 (1985)
STANDARD full automatic
REFERENCE 2 (bases 985 to 4265)
AUTHORS Stokes,H.W. and Hall,B.G.
TITLE Sequence of the ebgR gene of Escherichia coli: Evidence that the
EBG and LAC operons are descended from a common ancestor
JOURNAL Mol. Biol. Evol. 2, 478-483 (1985)
STANDARD full automatic
COMMENT SWISS-PROT; P06846; EGBR$ECOLI. SWISS-PROT; P06864; BGA2$ECOLI.
From EMBL 26 entry ECEBGRA; dated 26-APR-1991.
FEATURES Location/Qualifiers
CDS 1..981
/product="put. EBG repressor protein"
/gene="ebgA"
/codon_start=1
promoter 1189..1195
/note="pot. -10 region"
CDS 1371..4262
/product="evolved beta-galactosidase"
/gene="ebgR"
/codon_start=1371
BASE COUNT 1033 a 1173 c 1104 g 955 t
ORIGIN
1 atggcaacac taaaagacat cgcaatcgaa gctggcgtat ccctggcgac agtatccagg
61 gtcttaaatg acgatccgac attgaatgtg aaagaagaga cgaaacatcg cattctcgag
121 atcgccgaaa agctggagta caagaccagt agtgcccgta aactccagac aggtgcagtc
181 aaccaacacc atattctggc tatctacagc taccagcagg agctggagat caacgatcct
241 tactatctgg cgatccgcca cggcattgaa acccagtgcg aaaagctggg catcgagctc
301 accaactgtt atgaacacag cggcttacca gacattaaaa acgtcaccgg tattttaatt
361 gtcggcaaac ccacgcccgc cctgcgcgcc gctgccagcg cgtggaccga caatatctgt
421 tttatcgact ttcacgaacc cggcagcggt tacgatgcgg tggatatcga tctggcacgc
481 atcagtaaag aaatcatcga cttctatatc aaccagggcg ttaatcgtat tggttttatt
541 ggcggtgaag atgagcctgg caaggcggat attcgtgagg tcgcctttgc ggaatatggc
601 cgactgaaac aagtggtacg cgaagaggat atctggcgcg gcggtttttc cagttcgtcg
661 ggttatgaac tggcaaaaca aatgctggcg cgggaagact atccgaaggc actgtttgtt
721 gcttccgatt ccattgctat cggcgtactg cgggcaattc atgaacgagg cctgaacatc
781 ccacaggata tttcgcttat cagcgttaac gatatcccca ccgcgcgatt tacctttccg
841 ccgctctcca ccgtgcgcat ccattccgag atgatgggaa gtcagggcgt taacctggtg
901 tatgaaaaag cccgcgatgg tcgcgcgctg ccgctgttag tcttcgttcc cagcaaatta
961 aaactgcgcg gcacgacccg ttaaatcccc ttacacactg tccggcaatc gtttttgccg
1021 gacagtgctg ccgtttattt tcgtgatcca gttaaagtaa atgcatttac ctgctacttt
1081 ttagtaaaaa ttttactaaa ctccccagca attacacaaa ctaccatcac catgaatggt
1141 tccgatttct ctctaccggg aggccctatg aactcgctgg gaaaacattc agctcaccca
1201 cgaaaaccga cttgcgccgc gtgcgtactt tttttcacta tgattctgtt gcgcaacggc
1261 gtacctttgc ccgcgaaacc agcagcctgt ttctgccctt aagcggtcag tggaatttcc
1321 acttttttga ccatccgctg caagtaccag aagccttcac ctctgagtta atggctgact
1381 gggggcatat taccgtcccc gccatgtggc aaatggaagg tcacggcaaa ctgcaatata
1441 ccgacgaagg ttttccgttc cccatcgatg tgccgtttgt ccccagcgat aacccaaccg
1501 gtgcctatca acgtattttc accctcagcg acggctggca gggtaaacag acgctgatta
1561 aatttgacgg cgtcgaaacc tattttgaag tctatgttaa cggtcagtat gtgggtttca
1621 gcaagggcag tcgcctgacc gcagagtttg acatcagcgc gatggttaaa accggcgaca
1681 acctgttgtg tgtgcgcgtg atgcagtggg cggactctac ctacgtggaa gaccaggata
1741 tgtggtggtc agcggggatc ttccgcgatg tttatctggt cggaaaacac ctaacgcata
1801 ttaacgattt cactgtgcgt accgactttg acgaagccta ttgcgatgcc acgctttcct
1861 gcgaagtggt gctggaaaat ctcgccgcct cccctgtcgt cacgacgctg gaatataccc
1921 tgtttgatgg cgaacgcgtg gtgcacagca gcgccattga tcatttggca attgaaaaac
1981 tgaccagcgc cacgtttgct tttactgtcg aacagccgca gcaatggtca gcagaatccc
2041 cttatcttta ccatctggtc atgacgctga aagacgccaa cggcaacgtt ctggaagtgg
2101 tgccacaacg cgttggcttc cgtgatatca aagtgcgcga cggtctgttc tggatcaata
2161 accgttatgt gatgctgcac ggcgtcaacc gtcacgacaa cgatcatcgc aaaggccgcg
2221 ccgttggaat ggatcgcgtc gagaaagatc tccagttgat gaagcagcac aatatcaact
2281 ccgtgcgtac cgctcactac ccgaacgatc cgcgttttta cgaactgtgt gatatctacg
2341 gcctgtttgt gatggcggaa accgacgtcg aatcgcacgg ctttgctaat gtcggcgata
2401 ttagccgtat taccgacgat ccgcagtggg aaaaagtcta cgtcgagcgc attgttcgcc
2461 atatccacgc gcagaaaaac catccgtcga tcatcatctg gtcgctgggc aatgaatccg
2521 gctatggctg taacatccgc gcgatgtacc atgcggcgaa acggctggat gacacgcgac
2581 tggtgcatta cgaagaagat cgcgatgctg aagtggtcga tattatttcc accatgtaca
2641 cccgcgtgcc gctgatgaat gagtttggtg aatacccgca tccgaagccg cgcatcatct
2701 gtgaatatgc tcatgcgatg gggaacggac cgggcgggct gacggagtac cagaacgtct
2761 tctataagca cgattgcatt cagggtcatt atgtctggga gtggtgcgac cacgggatcc
2821 aggcacagga cgaccacggc aatgtctggt ataaattcgg cggcgactac ggcgactatc
2881 ccaacaacta taacttctgt cttgatggtt tgatctattc cgatcagacg ccgggaccgg
2941 gcctgaaaga gtacaaacag gttatcgcgc cggtaaaaat ccacgcgcgg gatctgactc
3001 gcggcgagtt gaaagtcgaa aataaactgt ggtttaccac gcttgatgac tacaccctgc
3061 acgcagaggt gcgcgccgaa ggtgaaagcc tcgcgacgca gcagattaaa ctgccggacg
3121 ttgcgccgaa cagcgaagcc cccttgcaga tcacgtgccg cagctggacg cccgcgaagc
3181 gttccctcaa cattacggtg accaaagatt cccgcacccg ctacagcgaa gccggacacc
3241 ctatcgccac ttatcagttc ccgctgaagg aaaacaccgc gcagccagtg cctttcgcac
3301 caaataaatg cgcgtccgtg acgctggaag acgatcgttt gagctgcacc gttcgcggct
3361 acaacttcgc gatcaccttc tcaaaaatga gtggcaaacc gacatcctgg caggtgaatg
3421 gcgaatcgct gctgactcgc gagccaaaga tcaacttctt caagccgatg atgatcgaca
3481 accacaagca ggagtacgaa gggctgtggc aaccgaatca tttgcagatc atgcaggaac
3541 atctgcgcga ctttgccgta gaacagagcg atggtgaagt gctgatcatc agccgcacag
3601 ttattgcccc gccggtgttt gacttcggga tgcgctgcac ctacatctgg cgcatcgctg
3661 ccgatggcca ggttaacgtg gcgctttccg gcgagcgtta cggcgactat ccgcacatca
3721 ttccgtgcat cggtttcacc atgggaatta acggcgaata cgatcaggtg gcgtattacg
3781 gtcgtggacc gggcgaaaac tacgccgaca gccagcaggc taacatcatc gatatctggc
3841 gccaagccgt cgatgccatg ttcgagaact atcccttccc gcagaacaac ggtaaccgtc
3901 agcatgtccg ctggacggca ctgactaacc gccacggtaa cggtctgctg gtggttccgc
3961 agcgcccaat taacttcagc gcctggcact atacccagga aaacatccac gctgcccagc
4021 actgtaacga gctgcagcgc agtgatgaca tcaccctagg aacctcgatc accagctgct
4081 tggcctcggc tccaactcct ggggcagcga ggtgctggac tcctggcgcg tctggttccg
4141 tgacttcagc tacggcttta cgttgctgcc ggtttctggc ggagaagcta ccgcgcaaag
4201 cctggcgtcg tatgagttcg gcgcagggtt cttttccacg aatttgcaca cggagaataa
4261 gctaa
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