GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS CORTRPLEG 1890 bp ds-DNA BCT 25-MAY-1991
DEFINITION C. glutamicum trpL and trpE genes
ACCESSION X55994
KEYWORDS TrpE gene; TrpL gene; anthranilate synthase.
SOURCE Corynebacterium glutamicum DNA.
ORGANISM Corynebacterium glutamicum
Prokaryota; Bacteria; Firmicutes; Irregular asporogenous rods.
REFERENCE 1 (bases 1 to 1890)
AUTHORS Heery,D.M.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Heery,D.M. and Dunican,L.K.
TITLE Nucleotide sequence of the Corynebacterium glutamicum trp E gene
JOURNAL Nucleic Acids Res. 18, 7138-7138 (1990)
STANDARD full staff_review
COMMENT *source: strain=ASO19 (ATCC13059);
Data kindly reviewed (28-JAN-1991) by Heery D.M.
From EMBL 26 entry CGTRPLEG; dated 27-APR-1991.
FEATURES Location/Qualifiers
mRNA 1..>1890
/gene="trpE"
-35_signal 15..22
-10_signal 38..45
RBS 70..73
CDS 83..136
/note="leader gene"
/product="putative leader peptide"
/gene="trpL"
/codon_start=83
attenuator 93..319
mat_peptide 334..1887
/product="anthranilate synthase component 1"
/gene="trpE"
CDS 334..1890
/product="anthranilate synthase component 1"
/gene="trpE"
/codon_start=334
BASE COUNT 400 a 551 c 497 g 442 t
ORIGIN
1 agagctgcgg aaactacgca agaacccaaa aatgattaat aattgagaca agcttcccac
61 ttatgtgata aagtcccatt ttgtgaataa ctcttgtctc agtcaaagca cccagtggtg
121 gtggcgcgct aactaagcga cctgacacct caagttgttt tcactttgat gaatttttta
181 aggctcgtac ttcgttcgac gaagaagcgg gccttttgtg gtttttagcc cacaaccggc
241 aagccctgga tcgaatgaag ctcgcagcga gtaattattt gatgtttccc agaaaggctt
301 cagccccaca atgatttcct cggtaggtgc cccatgagca cgaatcccca tgttttctcc
361 ctagatgtcc gctatcacga ggatgcttct gcgttgtttg cccacttggg tggcacaacc
421 gcagatgatg cagccctgtt ggaaagcgct gatatcacca ccaagaatgg tatttcttcc
481 ctcgcggtgt tgaagagttc ggtgcgcatt acgtgcacgg gcaacacggt ggtaacgcag
541 ccgctgacgg actcgggtag ggcagtggtt gcgcgcctaa cacagcagct tggccagtac
601 aacaccgcag agaacacctt tagcttcccc gcctccgatg cggttgatga gcgcgagcgc
661 ctcaccgcac caagcaccat cgaagtgctg cgcaagttgc agttcgagtc cggctacagc
721 gacgcgtccc tgccactgct catgggcggt ttcgcctttg atttcttaga aacctttgaa
781 acgctccccg ctgtcgagga gagcgtcaac acttaccccg attaccagtt cgtcctcgcg
841 gaaatcgtcc tggacatcaa tcaccaggac cagaccgcca aactcacggg cgtctccaac
901 gccccaggcg agctcgaggc cgagctcaac aagctttcat tgcttatcga cgccgccctc
961 cccgcaaccg aacacgccta ccaaaccacc cctcacgacg gcgacactct tcgcgttgtg
1021 gctgatattc ccgatgctca gttccgcacc cagatcaatg agctgaaaga aaacatttac
1081 aacggtgaca tctaccaagt tgtcccggcg cgcactttca ccgcaccatg tcctgatgca
1141 ttcgctgctt atctgcagct gcgtgccacc aacccgtcgc cgtacatgtt ctatatccgt
1201 ggactcaacg aaggtcgctc ctatgaactt tttggcgcat cccctgagtc caacctcaag
1261 ttcaccgctg ctaaccgtga gctgcagctg tacccaatcg caggtacccg cccccgtgga
1321 ctcaacccag atggctccat caacgatgag ctagatatcc gcaatgagtt ggatatgcgc
1381 actgatgcca aagagatcgc ggacgacacc atgcttgtcg atctcgcccg caacgacctg
1441 gcccgcgtct cggtcccagc gtcgcgccgg gttgcggatc ttttgcaggt ggatcgctat
1501 tcccgcgtga tgcacttggt gtcccgtgtg acggcgacgt tggacccaga gcttgatgct
1561 ttggacgcct atcgggcgtg catgaatatg ggcacgttga ccggcgctcc gaagttgcgc
1621 gctatggagc tgttgcgcgg cgtcgaaaag cgcaggcgtg gttcttatgg tggggcagtg
1681 gggtacctgc gcggcaatgg cgatatggat aattgcattg ttattcgttc ggcgtttgtc
1741 caggatggtg tggctgctgt gcaggctggt gctggtgtgg tccgcgattc taatcctcaa
1801 tctgaagccg atgagacgtt gcacaaggcg tatgccgtgt tgaatgccat tgcgcttgct
1861 gctggttcca ctttggaggt catccgatga
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