GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS PP4P4ER 3821 bp ds-DNA PHG 25-MAY-1991
DEFINITION Bacteriophage P4 (E.coli) essential region DNA ( early genes)
ACCESSION X02534
KEYWORDS overlapping genes; unidentified reading frame.
SOURCE Bacteriophage P4 DNA.
ORGANISM Bacteriophage P4
Viridae; ds-DNA nonenveloped viruses; Myoviridae.
REFERENCE 1 (bases 1 to 3821)
AUTHORS Lin,C.-S.
TITLE Nucleotide sequence of the essential region of bacteriophage P4
JOURNAL Nucleic Acids Res. 12, 8667-8684 (1984)
STANDARD full automatic
REFERENCE 2 (bases 1 to 3821)
AUTHORS Lin,C.S.
JOURNAL Unpublished (1985)
STANDARD full automatic
COMMENT SWISS-PROT; P05460; VPSU$BPP4. SWISS-PROT; P05461; VSID$BPP4.
SWISS-PROT; P05462; VASH$BPP4. SWISS-PROT; P05463; VEPS$BPP4.
SWISS-PROT; P05464; Y17K$BPP4. SWISS-PROT; P12551; VDEL$BPP4.
SWISS-PROT; P12552; Y9K$BPP4.
Data kindly reviewed (07-OCT-1985) by Lin C.-S.
From EMBL entry MYP4ER; dated 06-JUL-1989.
FEATURES Location/Qualifiers
CDS complement(<1..183)
/note="psu gene (aa 1-63)"
/codon_start=183
misc_feature complement(213..291)
/note="imp. region of dyad symmetry"
misc_feature complement(217..244)
/note="imp. region of dyad symmetry"
old_sequence 235..238
/note="GGCC was GCGC in [1]"
/citation=[1]
CDS complement(292..789)
/note="delta gene (aa 1-166)"
/codon_start=789
misc_feature complement(294..342)
/note="region of imp. dyad symmetry"
misc_feature complement(319..351)
/note="region of imp. dyad symmetry"
old_sequence 342..342
/note="T was absent in [1]"
/citation=[1]
misc_feature complement(368..411)
/note="region of imp. dyad symmetry"
misc_feature complement(380..439)
/note="region of imp. dyad symmetry"
mutation 478..478
/note="C is T in mutant delta 35"
CDS complement(757..1488)
/note="sid gene (aa 1-244)"
/codon_start=1488
mutation 783..783
/note="C is A in mutant sid 1"
misc_feature complement(817..862)
/note="region of imp. dyad symmetry"
mutation 841..841
/note="C is A in mutant sid 1"
misc_feature complement(1078..1109)
/note="region of imp. dyad symmetry"
RBS complement(1497..1499)
/note="pot. Shine Dalgarno sequence"
misc_feature complement(1570..1576)
/note="consensus -10 sequence"
misc_feature 1630..2041
/note="5' untranslated region"
misc_feature 1636..1697
/note="region of imp. dyad symmetry"
misc_feature 1645..1655
/note="put. ori consensus sequence"
repeat_region 1656..1675
/note="imp. direct repeat 1"
misc_feature 1711..1740
/note="region of imp. dyad symmetry"
repeat_region 1731..1751
/note="imp. direct repeat 1'"
repeat_region 1731..1742
/note="imp. direct repeat 2"
repeat_region 1752..1764
/note="imp. direct repeat 2'"
misc_feature 1799..1827
/note="region of imp. dyad symmetry"
misc_feature 1813..1845
/note="region of imp. dyad symmetry"
misc_feature 1830..1869
/note="region of imp. dyad symmetry"
repeat_region 1864..1875
/note="imp. direct repeat 3"
repeat_region 1883..1895
/note="imp. direct repeat 3'"
mutation 1972..1972
/note="C is T in mutant vir 1"
CDS 2041..2304
/note="unidentified reading frame (aa 1-88)"
/codon_start=2041
old_sequence 2283..2283
/note="A was absent in [1]"
/citation=[1]
misc_feature 2367..2372
/note="consensus -10 sequence"
misc_feature 2466..2505
/note="region of imp. dyad symmetry"
CDS 2490..2900
/note="put. ash gene (aa 1-137)"
/codon_start=2490
misc_feature 2553..2558
/note="consensus -10 sequence"
misc_feature 2570..2645
/note="region of imp. dyad symmetry"
mutation 2597..2597
/note="C is A in mutant ash 3"
repeat_region 2600..2610
/note="imp. direct repeat 4"
repeat_region 2616..2626
/note="imp. direct repeat 4'"
mutation 2623..2623
/note="T is A in mutant ash 9"
mutation 2624..2624
/note="T is C in mutant ash 8"
mutation 2625..2625
/note="C is T in mutant cI405"
mutation 2633..2634
/note="C is inserted in ash 10 and ash 26"
misc_feature 2805..2849
/note="imp. region of dyad symmetry"
mutation 2816..2816
/note="T is deleted in ash 26"
misc_feature 2861..2890
/note="imp. region of dyad symmetry"
CDS 2896..3180
/note="epsilon gene (aa 1-95)"
/codon_start=2896
mutation 2917..2917
/note="C is T in mutant epsilon am 104"
misc_feature 3062..3094
/note="imp. region of dyad symmetry"
misc_feature 3161..3190
/note="imp. region of dyad symmetry"
CDS 3176..3628
/note="unidentified reading frame (aa 1-151)"
/codon_start=3176
misc_feature 3214..3260
/note="imp. region of dyad symmetry"
misc_feature 3236..3283
/note="imp. region of dyad symmetry"
repeat_region 3481..3494
/note="imp. direct repeat 5"
repeat_region 3556..3569
/note="imp. direct repeat 5'"
misc_feature 3627..3632
/note="consensus -10 sequence"
misc_feature 3656..3690
/note="imp. region of dyad symmetry"
CDS 3767..>3821
/note="put. alpha gene (aa 1-18) (3819 is 1st base in
codon)"
/codon_start=3767
BASE COUNT 822 a 1056 c 977 g 966 t
ORIGIN
1 ctgccatttt ctgacttcga taatattgcg taattcgtcc aggcgactga cgtttctgcc
61 ttctcctgac agaagccgca gatattcctg ttcggccgct gccagctcat ttttgcgttg
121 cagccatgct gtcttgttat tctgacaggt gtcaaaggcc tgctgtaagg ctgtgctttc
181 catcgttatc tctttctcat catgctgaag aataaaaata cggtgtgcgg cgacggccgg
241 tgttaaccgg cagccctcat tccagacgca gcgaatatga ttgtgttttt aaccgtactg
301 gcggcagttc ctgtttttca tgcaggcgtt ctgccagttc gtccggcgtg accgggcgga
361 caatgaagcg gttgatggtc tgaagcgttt taaacaccag accacagccc ggatccgtgc
421 acacataaaa acgctcggtg acttcctgag acagacgccg cgatgttctt gacagtgcaa
481 ggcctttaca tctccgacaa caatatccgg taacaagcat tcttttcggg cgtttcatac
541 cgcaggaggc tgacgtcagt gaatcgcggt atctctgttt gcctgaaatg tattccattc
601 ctgaatcttt actgtcagag aaaaagcttt cactcgcttc aaatgtcgca gagcaataaa
661 tattccggca ctgtgcaatc attatcttgg tgccatcgtc catgaaatgt gcgcgacggg
721 tgtgagcaac atgtccacac gacggacagt aaatcatgac agcagccctc tggccttaag
781 ctctgctccc tgctggtcta ttttgtcctg ccacaccttg cgctgtgccg gtgtgcctgc
841 cacctcataa tccatgtgcg ggagtgttgc cgctgacagt ccggtcagcc ggagccaagg
901 ctcgccggtg aggctgattt gcatctgttt aattttctgt tccagcgatg atttcacctg
961 ctgcatgaca gccttttccg gtgcgacgta gccctgatgg ccggtggtgt tggcgagcgg
1021 attttcctgt accagaatgc tcagatgcat tgcccggaca agcgcctcac aggtttcatt
1081 cagggcgtgt tccagctcat gctctgcata cagactcaga aggtgatgat gtgccttccg
1141 gtaggcggtg gccgtgctgt cacacgcccc tttcaggcgt tcacgttcga aattcagcac
1201 cacggccaga ttgtcatatt cctgtaccag ctcccggcgt gccacgcgct caatgtggcg
1261 ctgtttcagc tcgtcgctca ggacaccacc ggctgcacga aaggccgtgc gccagtcgtc
1321 agcgtcgttt ccgtcggcct gcgccagcgc atttttttcc tgctctgccc gttcaatggc
1381 cgtgacggtc tcatccatca ggcgggcgtt ctcaagatgg gcggctctgg ccttttccag
1441 ttgtgccagt gcgggttgca gatattcagg gatagtgtgg tcagacattt tccggctcct
1501 cgtcacttca ggttaagaaa attgtgacgt acaccggaca acaacacgac gcattgcaga
1561 tgtgccagcc ctgacacagg agactcatcc tcagaccggc aagccaggaa aaggtcgcag
1621 gaaaaaccgg cttactgttt gtttttttat attttactgt tcacctctgt tcaccttaat
1681 aaaaaagata agtaatacag taagttaaag ggtgaacaat cgcagtaatg actgttcacc
1741 gtctgttcac cactgttcac ccgttgatgg gctttttgtg ctgtttacta ctgtttgctt
1801 ttattaattc gccgggaatg aataagaaga aacgatttgt atttcactat aaaaattaca
1861 aattgcttta agtggcttta aatgacttta agtggagatg aacaaaaaac acacagccat
1921 tgtaagacag cctgaacaaa tcccccctgt tgcgtctgct gaaaatattc acaaaataaa
1981 gcgctacccg aagccggacg gacttatccg gtgctgtatg gacattaacg aggtagcccg
2041 atgcaagctg ttttttcttc cccgtctccc gccccagtga cgccactgat gccgctgccg
2101 gacatcacgc aggagcgttt tttacgtgtg ccggaagtga tgcacctgtg cggcctgtca
2161 cgctcgacca tctacgaact catccgtaag ggggaatttc cgccgcaggt gagtcttggc
2221 ggtaaaaatg tggcctggct gcactctgaa gtcaccgcat ggatggccgg acgcattgcc
2281 ggacgcaaac gggggtacga cgcatgatga tgcccgctct gcaaaaactc cctttttctg
2341 gcttgccttt ttccggcatt tgcggatata gtttttcgcc tgccgcaaaa tcggcagccg
2401 ggcgtgagaa cccgagcaat tcacaggcga catatgacgc gccacgcgtc tttttttgtg
2461 tcgcaatcaa tgccacagag cgccagatta tggtgtggtg tgtggtcagt cgtgcagatg
2521 gcattccgtg catcctgccc gcgtccgctc attatgccgc agagtcaatg gtagctcagg
2581 cggggcagcc tccgggctgg ccggtatcct gtgaagccgg tattctcacc cccgtctggg
2641 ctatcgccat cgagcgtgag aactccggcg atagcgttat ttgctattca caggaggctg
2701 ccatcatggc tacaaccctt accccctcac accctgaatt tgtctttgtg tttgcggctg
2761 tccgtcgcgc agaccgtcat ccccgtatct gtatgcttcg caccgtcgcc ggtgatgaac
2821 gcagtgcccg ccgttccctt gtccgtgact atgtgctctc ccttgctgcc cgtctgccgg
2881 tggtggaggt gtcccgtgcg taataaaaaa gcccctcaga ccgtctcagc gcgtcatgac
2941 gcccgtgaac acctcagcat tgaggcttac cataagctca accgcgccag cgccgtatcc
3001 cggtttgttg ggggtgattt gattcaccgt gaactctccg gcctgcatca gctctacatt
3061 ccacatattt tcagctacct gaatgaagat attgattttg tgctgaatga gctgaaagcc
3121 aaaggcctgt gccgcgattt tctcgcccag cagaaagacc ggggagacag gacgcatgtt
3181 tgattttccc cagcccggtg agatttaccg ttctgccggt tttcccgatg tggccgtggt
3241 cggcattctg gaagacggta ttccgtggga aatgccgtac cgctgcccgg acattgtctg
3301 gaacccgtac cgccgtaaat tcagtatcct tgtgcgtatc ctcgctgacg gacgcaccac
3361 agacatcccg ctggggcgtt ttctgcggga atttacctgt gaccgtcctg acctgtttaa
3421 acgcagcccc gtaaaccggc atgcggtact gaaagaaatg gccggagacc cggaattaca
3481 gaaatggcgg gaaaaatatc tggatattta cccgcaggac actgttccgg tcagccgggc
3541 agcaccggtg gcgcgggaat ggcgggaaat tccccgcacg gagcctgacc cggaaaccac
3601 cccggataac agttaccgca attatctgta attaaaaaac gacccccgaa aaattaaatg
3661 tgcgtattcg cgcagggata cgcacgtctt caggagacgc agatatgcct tatcagttaa
3721 tgcaaccggc acggaatgca gtcatctgtc acagggagaa aagcaaatga aaacaccctt
3781 accgcccgtc ttacgcgctg ccctttaccg tcgcgctgtc g
//