GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS PT4T4NRDB 3058 bp ds-DNA PHG 25-MAY-1991
DEFINITION Bacteriophage T4 gene nrdB for ribonucleotide reductase small
subunit (B2)
ACCESSION X04140
KEYWORDS RNA ligase; endonuclease II; ligase; nrdB gene; reductase;
ribonucleotide reductase; unidentified reading frame.
SOURCE Bacteriophage T4 DNA.
ORGANISM Bacteriophage T4
Viridae; ds-DNA nonenveloped viruses; Myoviridae.
REFERENCE 1 (sites)
AUTHORS Rand,K.N. and Gait,M.J.
TITLE Sequence and cloning of bacteriophage T4 gene 63 encoding RNA
ligase and tail fibre attachment activities
JOURNAL EMBO J. 3, 397-402 (1984)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 3058)
AUTHORS Sjoeberg,B.M., Hahne,S., Mathews,C.Z., Mathews,C.K., Rand,K.N. and
Gait,M.J.
TITLE The bacteriophage T4 gene for the small subunit of ribonucleotide
reductase contains an intron
JOURNAL EMBO J. 5, 2031-2036 (1986)
STANDARD full automatic
COMMENT SWISS-PROT; P00971; RLIG$BPT4. SWISS-PROT; P07059; END2$BPT4.
SWISS-PROT; P11156; RIR2$BPT4.
Positions of exon 1 and exon 2 boundaries have been revised Belfort
M. (personal communication); Sjoeberg B-M. (unpublished result)
Data kindly reviewed (19-OCT-1986) by B-M. Sjoeberg
From EMBL entry MYT4NRDB; dated 06-JUL-1989.
FEATURES Location/Qualifiers
promoter 490..496
/note="pot. -35 region"
promoter 508..513
/note="pot. -10 region"
RBS 527..530
/note="pot. ribosome binding site"
CDS 537..1200
/note="small subunit B2 (aa 1-221) exon 1"
/codon_start=537
intron 1201..1798
/note="intron I"
CDS 1370..1660
/note="URF (aa 1-97)"
/codon_start=1370
CDS 1799..2298
/note="small subunit B2 (aa 222-388) exon 2"
/codon_start=1799
RBS 2301..2304
/note="pot. ribosome binding site"
RBS 2314..2317
/note="pot. ribosome binding site"
CDS 2329..2736
/note="put. endonuclease II (denA) (aa 1-136)"
/codon_start=2329
RBS 2782..2785
/note="pot. ribosome binding site"
CDS 2792..>3058
/note="RNA ligase (gene 63)"
/codon_start=2792
BASE COUNT 1062 a 501 c 592 g 903 t
ORIGIN
1 gcatgctatt ttatcaaaga tttatccggt aaaatctgtt atattcgcat ttttcatgat
61 gtgcaatatg aaaggaacta agaaacgtca ttataaagtt cattctaaaa tatatgccca
121 tgcaaagaag ttaaattcgc aatttcgcaa aggtacggtc atttctgaag aaacgagact
181 gaaaatgtca aaaagcgaaa acaggtctgc gtttaacaga agaaaccaaa cacaaaatat
241 cggcggcaac caaaggaagg gctaaaagcg aagaaaccta agaaaaagat gagaaagcct
301 aaaactgaag aagctaaaaa gaatatagct gctgctaaag tcggcgtgct taatccgatg
361 tatggtacaa tttctccgac aagagatgtt cctcatacta aagaaacccg tgatttgatt
421 tctttgagaa ctaaacaagg agcagagtat ccaccttgcc cgcactgtgg taagaaagtt
481 aataaaggca atgctttaag atggcattat gataaatgta aatttaagga ttctaaatga
541 gtacagtttt taatacaaat ccagttgatg ttttgaatga accgatgttc tttggttctg
601 gattaggttt agctcgatat gatattcaac gacatcgagt atttgaagaa ctcattgagc
661 ggcagatcag ttttttttgg cgtcctgaag aagtaaactt aatgatggat gctgcgcaat
721 ttaataagct tcctcagtat cagcaaaata tttttactaa caacctgaag tatcaatcac
781 ttttggatag cattcagggt cgtgcgccat ctgctgtact tatgtcatta atttcagacc
841 caagccttga tacatgggtt gctacatgga cttttagtga aactattcac agtcgttcat
901 atactcatat catgcgaaat ctttatactg atccatcgaa ggtgtttgat gagattgtat
961 tagatgaagc tattatgaaa cgtgctgagt ccattgggcg atattacgat gatgtcttgg
1021 ttaaaacccg tgaatgggaa aatgctaaag acatggttga atattataaa gaccaaggtc
1081 taattctagc tgataaagac gttgagcaaa gagctaaacg agatctgatc aaatctcttt
1141 acctctgttt gcacgttatt aatgccttag aagctattcg tttttatgta tcttttgcgt
1201 aaaatgcgcc tttaaacggt aacgtttatc gaaaactcct ttaattgctg gaaagtcctt
1261 tatggaaaac tagcagccaa ggttttgctt gcacctttaa ttagtataat ataaatatga
1321 ttatactttt aagaggatgc acaattgaac tatagaaaaa tttggataga tgctaacgga
1381 cctataccta aagattctga tggaagaaca taagaaaaga acatcagcaa aaacattaga
1441 aatggttaag aacgggaccc atcctgcaca aaaagaaatc acatgtgatt tctgtggtca
1501 cattggtaaa ggtcctggat tttatctaaa acataatgac agatgtaaat taaatccaaa
1561 tagaattcaa ttgaattgtc catattgtga taagaaagat ttatcaccat caacatataa
1621 acgatggcac ggcgacaatt gcaaaacaag gttcaacgac tagtcttcgg acgtagggtc
1681 aagcgactcg aaatggggag aatccctccg ggattgtgat atagtctgga ctgcatggta
1741 acatgcagca gttcataaga gaacgggttg agaattagcg agctcaatcg aacatacggt
1801 acctttaact tccataagaa catggaaatc atggaaggta atgccaagat tatgaagttc
1861 attgcacgtg atgaacagct tcaccttaaa ggcacccaat atattattcg tcaacttcaa
1921 tccggtactg atggtgatga atgggttaaa attgcccaag agtgtgaaca agaagcagtt
1981 gatattttca tggaagttaa ccgccaggaa aaagactggg cagttcattt atttaaagat
2041 ggtgatgttc ctggattaaa tacaaatagc atgtggagct ttattgatta cttaactgta
2101 tctcgtatga aacagtgcgg tcttccatgc ccaattaccg atgctccggt taaacatcca
2161 tatccttgga ttcgtgaata tcttaattct gataatgttc aatccgcgcc gcaagaagta
2221 gaactgtcat cttaccttgt tgcacagatt gataatgatg ttgatgataa agttatgatg
2281 agttttaaaa aatactttta aggagtgggc cgcaaggccc attttattat gaaagaaatt
2341 gcaacagaat attcatttat taaatatact gagctagaat tagacgacaa tggaagtata
2401 aaacaattat ctattccaaa caagtataac gtaatttatg ctattgctat aaatgatgag
2461 cttgtttata ttggaaaaac taaaaattta cgtaaaagaa taaactatta tagaactgct
2521 attaaccgca aagacaaaac gtctgattct actaaatctg cattaattca ttctgcgcta
2581 aaggaaggaa gcaaagttga attttacgcc cgccaatgtt ttaatctttc tatgacaaat
2641 gagttaggta caatgacaat cgcaacgatt gacttagagg agcctctatt cattaaactg
2701 tttaacccgc cttggaatat tcaacacaag aaaaaatgat gcttccacat ggagtgtggt
2761 actatattca aaacacaaaa gaggatacac aatgcaagaa ctttttaaca atttaatgga
2821 actatgtaag gattcgcagc gtaagttttt ttactcagat gatgtaagtg catctggaag
2881 aacttacaga attttctcat ataattatgc atcttattct gattggttac ttccagatgc
2941 actagaatgt cgtggaatta tgtttgaaat ggatggagaa aaaccagtaa gaattgcttc
3001 tcgtcctatg gaaaagtttt ttaacttgaa tgaaaatccg ttcacgatga atatcgat
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