GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS PT4T4TRN 5015 bp ds-DNA PHG 25-MAY-1991
DEFINITION Bacteriophage T4 tRNA region genes 3 (3' term), 57A, 57B, IpI; RNA
subcluster I and II
ACCESSION X03016 V00861 V00862
KEYWORDS capsid protein; terminator; transfer RNA; transfer RNA-Arg;
transfer RNA-Gln; transfer RNA-Gly; transfer RNA-Ile;
transfer RNA-Leu; transfer RNA-Pro; transfer RNA-Ser;
transfer RNA-Thr; unidentified reading frame.
SOURCE Bacteriophage T4 DNA.
ORGANISM Bacteriophage T4
Viridae; ds-DNA nonenveloped viruses; Myoviridae.
REFERENCE 1 (bases 3251 to 3830)
AUTHORS Fukada,K. and Abelson,J.
TITLE DNA Sequence of a T4 Transfer RNA Gene Cluster
JOURNAL J. Mol. Biol. 139, 377-391 (1980)
STANDARD full automatic
REFERENCE 2 (bases 3544 to 3842; 4416 to 5015)
AUTHORS Mazzara,G.P., Plunkett,G.I. and McClain,W.H.
TITLE DNA sequence of the transfer RNA region of bacteriophage T4:
Implications for transfer RNA synthesis
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 78, 889-892 (1981)
STANDARD full automatic
REFERENCE 3 (bases 852 to 1488; 2762 to 2817)
AUTHORS Herrmann,R.
TITLE Nucleotide sequence of the bacteriophage T4 gene 57 and a deduced
amino acid sequence
JOURNAL Nucleic Acids Res. 10, 1105-1112 (1982)
STANDARD full automatic
REFERENCE 4 (bases 1 to 5015)
AUTHORS Broida,J. and Abelson,J.
TITLE Sequence organization and control of transcription in the
bacteriophage T4 tRNA region
JOURNAL J. Mol. Biol. 185, 545-563 (1985)
STANDARD full automatic
COMMENT SWISS-PROT; P03718; VIN1$BPT4. SWISS-PROT; P04531; KDNM$BPT4.
SWISS-PROT; P04532; V57A$BPT4. SWISS-PROT; P04533; V57B$BPT4.
SWISS-PROT; P13322; YTR2$BPT4. SWISS-PROT; P13323; YTR3$BPT4.
SWISS-PROT; P13324; YTR4$BPT4. SWISS-PROT; P13325; YTR5$BPT4.
SWISS-PROT; P13331; VG03$BPT4.
From EMBL entry MYT4TRN; dated 18-NOV-1986.
FEATURES Location/Qualifiers
CDS <1..262
/note="3' end of gene 3 (phage morphogenesis)"
/codon_start=1
misc_feature 361..367
/note="early promoter consensus sequence"
promoter 363..369
/note="put. -35 region (PM1)"
promoter 382..387
/note="put. -10 region (PM1)"
CDS 413..1135
/note="gene 1 (aa 1-241)"
/codon_start=413
misc_signal 571..601
/note="pot. stem-loop structure, pot. terminator"
misc_feature 775..783
/note="pot. -10 region (PL1)"
misc_feature 782..847
/note="pot. stem-loop structure"
misc_feature 1041..1070
/note="pot. stem-loop structure"
CDS 1138..1377
/note="put. 57A protein (aa 1-80)"
/codon_start=1138
CDS 1380..1832
/note="put. 57B protein (ORF1) (aa 1-151)"
/codon_start=1380
promoter 1849..1855
/note="put. -35 region (PE2)"
misc_feature 1850..1894
/note="pot. stem-loop structure"
promoter 1872..1877
/note="put. -10 region (PE2)"
CDS 1909..2193
/note="internal capsid protein (Ip)I precursor"
/codon_start=1909
CDS 1954..2193
/note="mature IpI (aa 1-80)"
/codon_start=1954
misc_feature 2213..2250
/note="pot. stem-loop structure pot. IpI terminator"
promoter 2216..2222
/note="put. -35 region (PE1)"
promoter 2239..2245
/note="put. -10 regin (PE1)"
CDS 2273..2455
/note="ORF2, unidentified reading frame"
/codon_start=2273
CDS 2459..2866
/note="ORF3, unidentified reading frame"
/codon_start=2459
CDS 2872..3156
/note="ORF4; unidentified reading frame"
/codon_start=2872
promoter 2911..2918
/note="pot. -10 region (PL2)"
misc_feature 3094..3134
/note="pot. stem-loop structure"
promoter 3161..3167
/note="put. -35 region (PM2)"
promoter 3182..3187
/note="put. -10 region (PM2)"
tRNA 3257..3330
/note="tRNA-Gln"
tRNA 3331..3417
/note="tRNA-Leu"
tRNA 3423..3496
/note="tRNA-Gly"
tRNA 3507..3581
/note="tRNA-Pro"
tRNA 3583..3669
/note="tRNA-Ser"
tRNA 3677..3752
/note="tRNA-Thr"
tRNA 3754..3827
/note="tRNA-Ile"
conflict 3828..3829
/note="T is inserted in ref [4]"
/citation=[2]
misc_feature 3835..3875
/note="pot. stem-loop structure; pot. terminator"
CDS 3925..4530
/note="unidentified reading frame (ORF5)"
/codon_start=3925
tRNA 4539..4613
/note="tRNA-Arg"
misc_feature 4615..4734
/note="species 2 RNA"
misc_feature 4741..4877
/note="species 1 RNA"
misc_feature 4826..4851
/note="pot. stem-loop structure"
misc_feature 4929..4952
/note="pot. stem-loop structure; pot. terminator"
BASE COUNT 1611 a 806 c 1012 g 1586 t
ORIGIN
1 ggccagcaca actccagtgg ttgttacatt tgagtatgat ccacagattg tgagatttat
61 agttgatgaa gaacttaagt cgtggatagg aatgtatgaa tggatgctag gaactagcaa
121 ctatcttaca ggtgaaaata ctgcccaaaa aacaggtcct gagtacatta cgctttacat
181 cttagataat agcaaaactg aaatcgtgat gtcaataaat ttttataagc cttgggtttc
241 tgacctatct gaagtaagaa tttagctaca cggaagattc agacccggtt tagtatgtac
301 tgcaacaatt ccttacacct attttcaagt agaaaaagat ggtaaaatta tagcagaagt
361 ttaatgcttc agtttgtgtg ttataatctt aattaaattt gaggagaaac acatgaaact
421 aatcttttta agcggtgtaa agcgtagtgg aaaagatact actgctgatt ttatcatgag
481 caattattct gcagttaaat accaacttgc tggtcctatt aaggatgcat tggcttatgc
541 atggggagta tttgcagcaa acactgacta tccttgctta actcgtaaag agtttgaagg
601 aattgactat gatcgtgaga ctaatttaaa tctgactaaa ttagaagtaa tcacgattat
661 ggaacaagca ttttgttatc ttaatggtaa aagcccaatt aaaggtgtgt ttgtttttga
721 tgacgaagga aaagaatcag ttaatttcgt agcatttaat aagattactg acgttataaa
781 taatattgaa gatcaatggt cagtccgtcg tctgatgcaa gccctaggta cggatttgat
841 tgttaataac ttcgaccgca tgtactgggt aaaattattt gctttagatt atcttgataa
901 atttaactca ggttatgatt attatatcgt tcctgatacc cgtcaagatc atgaaatgga
961 tgcggctagg gcgatgggtg ctacagtaat tcatgtagtt cgtcctggtc aaaaatccaa
1021 tgatacacat attacagaag ctggattgcc aattcgtgat ggcgatttag taattacaaa
1081 cgatggttct cttgaagaac ttttttctaa aattaaaaat acactaaagg tactataatg
1141 tctgaacaaa ctgttgaaca aaaactgtct gctgaaatcg taactctgaa atctcgcatt
1201 cttgatacgc aggatcaagc tgctcgtctg atggaagaat ccaaaattct gcaaggaact
1261 ttggctgaaa ttgctcgtgc agtaggtatc actggcgata ctatcaaagt tgaagaaatc
1321 gttgaagctg tcaaaaatct tactgctgaa tctgcagatg aagcaaaaga tgaagaatga
1381 tggaatttaa agacttttca acgggtcttt atgtagcagc taagttttca gaattaacac
1441 ttgatgcgct ggaagaactt cagcgctctt tacgtgttcc taatccagtt cctagagaaa
1501 aaattcattc gactatatgt tattcaagag taaatgttcc atatgttcca tcgagtggaa
1561 gttttgaagt agcttcttct ggacatttag aagtatggaa aacacaagat ggatcgactc
1621 ttgtacttgt gctagattct gaatatctgc gctgtcgaca catgtatgcg cgggcactag
1681 gtgctacaca tgattttgat gattacacac cgcatataac attgtcttat aatgttgggc
1741 ccttatcatt tagcggtgat gtacaaattc cggtcgtatt agatcgtgaa tacaaagagc
1801 ctcttaaact cgattgggca gatgatttaa aataatttca caaagttgtt tacatactga
1861 tgaggtagtg atactattac ctcatcaaaa ttaattagga aaataaaaat gaaaacattt
1921 aaagaattta cttctacaac taccccggtt tctaccatta ccgaagctac tcttacttct
1981 gaagttatta aagcaaataa aggacgagaa ggtaaaccaa tgattagtct ggttgatggc
2041 gaagaaatca aaggtactgt ttacctaggt gatgggtggt ctgctaaaaa ggatggtgct
2101 acaatcgtta tctctcctgc tgaagaaact gcgttgttta aagctaaaca catttctgcg
2161 gcacatctca agattattgc taaaaatctt ttgtaataaa tatttcacaa agttgtttac
2221 atagggtttt agctgtgata ctattaccct atcaactact gaggagaata aaatgaaacg
2281 ctgtgaatta attcgaaatg ttgctattgc aatttctgct tctgctttta gtttttcaat
2341 gtttgttgga tttatatgcg gattattaac tacagcagaa aatgtgtttt cacttgtagt
2401 agcattttta attggtttaa ttgctatcgt tatggataaa atttctaaag gtgaataaat
2461 gagtattaac gaatattatg tttatgccgg ggattatgca aatccttctc attttgaagg
2521 taatttaata cctgataaag tttttaatac tccttttgaa gcgtggagtt ggattgaaag
2581 taaaaatgga ttttcttacc gttatgttga agtaactgat tggagaggaa ctaaatatcc
2641 taaagaccat tattacgtaa atccatctaa ggtaaatttt cttttattcg caggtgataa
2701 ttattatcct tgtggtggat atgatgattt aattgcgtat gctgaaacag aagataaact
2761 gcgtgatatc attaaagaaa atgaaaataa accagattat ggttccaatc gtttcgactg
2821 gtggcaaatc gtaaatgccc atactcatgc tattgttgat agaggttgat gatgattctt
2881 tatgcgaaag tatcatccgt tgaaaatgga tataaatatg atcaagaagc agctaaagcc
2941 ttgattgatg attatggcat tttaacgtgt tttgaagttg aaaaggttta cattgatcgt
3001 tcatcttctc aagttaaatt agtaaaggaa gaccgtaaat ttaatacagt aaattttgat
3061 ttctttattg aaacagaaaa aggtcctctt gaatatgata ttttcaagaa tcctttgggt
3121 cttgaatgta tcgtaaatac gtatcatcat aaatggtaaa tatgctttaa gaattatttg
3181 ttattattaa ctcataccgc actgattaat accctctatc atcaagggtt cttgttagag
3241 agcctttgtt aataattggg aattagccaa gttggtaagg catagcactt tgactgctag
3301 atgcaaaggt tcgagtcctt tattcccagc gcgagaatgg tcaaattggt aaaggcacag
3361 cacttaaaat gctgcggaat gatttccttg tgggttcgag tcccacttct cgcaccaaat
3421 ttgcggatat cgtataatgg tattacctca gacttccaat ctgatgatgt gagttcgatt
3481 ctcattatcc gctccaattt aatttactcc gtgtagctca gtttggtaga gcgcctgatt
3541 tgggatcagg aggtccaagg ttcaaatcct tgtatggaga ctggaggcgt ggcagagtgg
3601 tttaatgcac cggtcttgaa aaccggcagt cgctccggcg actcataggt tcaaatccta
3661 tcgcctccgt aattttgctg atttagctca gtaggtagag cacctcactt gtaatgagga
3721 tgtcggcggt tcgattccgt caatcagcac caaggccctg tagctcaatg gttagcagca
3781 gtcccctcat aagggaaagg ttaccagttc aaatctggtc tgggtcatat tttgagaaca
3841 taaatactct tagaaatagg agatatttat gttctattac attataaaat aacaaataaa
3901 attaataata aaatttatat tggtgtgcat tctaccgaaa atttagatga tggatatatg
3961 ggttctggta aattattaaa aagagcccaa gataaatatg gtatagaaaa tttttctaaa
4021 gaaatattag agtattttga cgataaagaa tctatgcttg aagccgagaa aaatattgtc
4081 acagaagagt ttttaaatag accagatgtg tataatttaa aacttggcgg tgaaggtgga
4141 tgggatcatg taaatattcc tggaatgcta aaccagaaga aagatgctag cttaaaagga
4201 gctaaatcgt ttaagtcacg ttttgaaaat gatattttac ttcaagaaaa atatcgtaaa
4261 attggttcaa atgtatttaa acgtttgtgg tcaactcctg aatacaggga aaaattttta
4321 aataatagca gatttctaaa taagcatcat actcctgaaa ctattaataa aatgaaagaa
4381 tctcacgcca aaaataatca ccagaaaggt gaaaagaatt cccaattcgg tatgatgtgg
4441 attcattcat tagatgaaaa agtttctaaa cgtattaaga aaactgaccc aattccagaa
4501 ggatggttta aaggtcgaaa aatgaaattt taaattacgt cccgctggtg taatggatag
4561 catacgatcc ttctaagttt gcggtcctgg ttcgatccca gggcgggata ccaaatgaga
4621 aaccgggtcg ctaccggtaa gtcgtcggac tgatggttcc ctgagtaagg aattgcgtta
4681 ataatctttg cgtttattga tgccctctta catcacagca gaaacggcgc accaaattat
4741 cgattcgagg aaatatcttt gccgtaagcc gagtagcgtt tttgacggaa cgttcggata
4801 tggttgagat atggcctttt aaaaatattg agtagcgtca actacttaat aaccgggttc
4861 gaatcccggc gtttcgtaca aacacttgcc ttggcaggtg gaaccccgac aaggttgccg
4921 caacgcttag ccccgaccga aaggttgggg ctttttggat atctaagcct ttccagacct
4981 ctctaggcta catttagttt atacccttta taata
//