GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS P29TS224 1728 bp ds-DNA PHG 25-MAY-1991
DEFINITION Bacteriophage phi29 temperature sensitive mutant TS2(24) DNA
polymerase gene
ACCESSION X53371
KEYWORDS DNA polymerase; polymerase.
SOURCE Bacteriophage phi-29 DNA.
ORGANISM Bacteriophage phi-29
Viridae; ds-DNA nonenveloped viruses; Podoviridae.
REFERENCE 1 (bases 1 to 1728)
AUTHORS Bernad,A.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1728)
AUTHORS Blasco,M.A., Blanco,L., Pares,E., Salas,M. and Bernad,A.
TITLE Structural and functional analysis of temperature-sensitive mutants
of the phi 29 DNA polymerase
JOURNAL Nucleic Acids Res. 18, 4763-4770 (1990)
STANDARD full automatic
COMMENT *source: strain=TS2(24); clone=pABts2(24); **map: map position=
2872-1145; See <V01155> for wild type sequence.
From EMBL entry POTS224; dated 31-AUG-1990.
FEATURES Location/Qualifiers
precursor_RNA <1..>1728
/note="transcript"
CDS 1..1725
/note="DNA polymerase (AA 1-575)"
/codon_start=1
mutation 526..527
/note="CG is GC in <V01155>"
mutation 780..780
/note="G is A in <V01155>"
mutation 1064..1064
/note="A is G in <V01155>"
BASE COUNT 612 a 262 c 395 g 459 t
ORIGIN
1 atgaagcata tgccgagaaa gatgtatagt tgtgactttg agacaactac taaagtggaa
61 gactgtaggg tatgggcgta tggttatatg aatatagaag atcacagtga gtacaaaata
121 ggtaatagcc tggatgagtt tatggcgtgg gtgttgaagg tacaagctga tctatatttc
181 cataacctca aatttgacgg agcttttatc attaactggt tggaacgtaa tggttttaag
241 tggtcggctg acggattgcc aaacacatat aatacgatca tatctcgcat gggacaatgg
301 tacatgattg atatatgttt aggctacaaa gggaaacgta agatacatac agtgatatat
361 gacagcttaa agaaactacc gtttcctgtt aagaagatag ctaaagactt taaactaact
421 gttcttaaag gtgatattga ttaccacaaa gaaagaccag tcggctataa gataacaccc
481 gaagaatacg cctatattaa aaacgatatt cagattattg cggaacgtct gttaattcag
541 tttaagcaag gtttagaccg gatgacagca ggcagtgaca gtctaaaagg tttcaaggat
601 attataacca ctaagaaatt caaaaaggtg tttcctacat tgagtcttgg actcgataag
661 gaagtgagat acgcctatag aggtggtttt acatggttaa atgataggtt caaagaaaaa
721 gaaatcggag aaggcatggt cttcgatgtt aatagtctat atcctgcaca gatgtatagc
781 cgtctccttc catatggtga acctatagta ttcgagggta aatacgtttg ggacgaagat
841 tacccactac acatacagca tatcagatgt gagttcgaat tgaaagaggg ctatataccc
901 actatacaga taaaaagaag taggttttat aaaggtaatg agtacctaaa aagtagcggc
961 ggggagatag ccgacctctg gttgtcaaat gtagacctag aattaatgaa agaacactac
1021 gatttatata acgttgaata tatcagcggc ttaaaattta aagtaactac aggtttgttt
1081 aaagatttta tagataaatg gacgtacatc aagacgacat cagaaggagc gatcaagcaa
1141 ctagcaaaac tgatgttaaa cagtctatac ggtaaattcg ctagtaaccc tgatgttaca
1201 gggaaagtcc cttatttaaa agagaatggg gcgctaggtt tcagacttgg agaagaggaa
1261 acaaaagacc ctgtttatac acctatgggc gttttcatca ctgcatgggc tagatacacg
1321 acaattacag cggcacaggc ttgttatgat cggataatat actgtgatac tgacagcata
1381 catttaacgg gtacagagat acctgatgta ataaaagata tagttgaccc taagaaattg
1441 ggatactggg cacatgaaag tacattcaaa agagctaaat atctgagaca gaagacctat
1501 atacaagaca tctatatgaa agaagtagat ggtaagttag tagaaggtag tccagatgat
1561 tacactgata taaaatttag tgttaaatgt gcgggaatga ctgacaagat taagaaagag
1621 gttacgtttg agaatttcaa agtcggattc agtcggaaaa tgaagcctaa gcctgtgcaa
1681 gtgccgggcg gggtggttct ggttgatgac acattcacaa tcaaataa
//GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS P29TS298 1728 bp ds-DNA PHG 25-MAY-1991
DEFINITION Bacteriophage phi29 temperature sensitive mutant TS2(98) DNA
polymerase gene
ACCESSION X53370
KEYWORDS DNA polymerase; polymerase.
SOURCE Bacteriophage phi-29 DNA.
ORGANISM Bacteriophage phi-29
Viridae; ds-DNA nonenveloped viruses; Podoviridae.
REFERENCE 1 (bases 1 to 1728)
AUTHORS Bernad,A.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1728)
AUTHORS Blasco,M.A., Blanco,L., Pares,E., Salas,M. and Bernad,A.
TITLE Structural and functional analysis of temperature-sensitive mutants
of the phi 29 DNA polymerase
JOURNAL Nucleic Acids Res. 18, 4763-4770 (1990)
STANDARD full automatic
COMMENT *source: strain=TS2(98); clone=pABts2(98); **map: map position=
2872-1145; See <V01155> for wild type sequence.
Data kindly reviewed (22-OCT-1990) by Bernad A.
Data kindly reviewed (22-OCT-1990) by Bernad A.
From EMBL entry POTS298; dated 19-NOV-1990.
FEATURES Location/Qualifiers
precursor_RNA <1..>1728
/note="transcript"
CDS 1..1725
/note="DNA polymerase (AA 1-575)"
/codon_start=1
mutation 526..527
/note="CG is GC in <V01155>"
mutation 780..780
/note="G is A in <V01155>"
mutation 1475..1475
/note="A is G in <V01155>"
BASE COUNT 612 a 262 c 395 g 459 t
ORIGIN
1 atgaagcata tgccgagaaa gatgtatagt tgtgactttg agacaactac taaagtggaa
61 gactgtaggg tatgggcgta tggttatatg aatatagaag atcacagtga gtacaaaata
121 ggtaatagcc tggatgagtt tatggcgtgg gtgttgaagg tacaagctga tctatatttc
181 cataacctca aatttgacgg agcttttatc attaactggt tggaacgtaa tggttttaag
241 tggtcggctg acggattgcc aaacacatat aatacgatca tatctcgcat gggacaatgg
301 tacatgattg atatatgttt aggctacaaa gggaaacgta agatacatac agtgatatat
361 gacagcttaa agaaactacc gtttcctgtt aagaagatag ctaaagactt taaactaact
421 gttcttaaag gtgatattga ttaccacaaa gaaagaccag tcggctataa gataacaccc
481 gaagaatacg cctatattaa aaacgatatt cagattattg cggaacgtct gttaattcag
541 tttaagcaag gtttagaccg gatgacagca ggcagtgaca gtctaaaagg tttcaaggat
601 attataacca ctaagaaatt caaaaaggtg tttcctacat tgagtcttgg actcgataag
661 gaagtgagat acgcctatag aggtggtttt acatggttaa atgataggtt caaagaaaaa
721 gaaatcggag aaggcatggt cttcgatgtt aatagtctat atcctgcaca gatgtatagc
781 cgtctccttc catatggtga acctatagta ttcgagggta aatacgtttg ggacgaagat
841 tacccactac acatacagca tatcagatgt gagttcgaat tgaaagaggg ctatataccc
901 actatacaga taaaaagaag taggttttat aaaggtaatg agtacctaaa aagtagcggc
961 ggggagatag ccgacctctg gttgtcaaat gtagacctag aattaatgaa agaacactac
1021 gatttatata acgttgaata tatcagcggc ttaaaattta aagcaactac aggtttgttt
1081 aaagatttta tagataaatg gacgtacatc aagacgacat cagaaggagc gatcaagcaa
1141 ctagcaaaac tgatgttaaa cagtctatac ggtaaattcg ctagtaaccc tgatgttaca
1201 gggaaagtcc cttatttaaa agagaatggg gcgctaggtt tcagacttgg agaagaggaa
1261 acaaaagacc ctgtttatac acctatgggc gttttcatca ctgcatgggc tagatacacg
1321 acaattacag cggcacaggc ttgttatgat cggataatat actgtgatac tgacagcata
1381 catttaacgg gtacagagat acctgatgta ataaaagata tagttgaccc taagaaattg
1441 ggatactggg cacatgaaag tacattcaaa agagttaaat atctgagaca gaagacctat
1501 atacaagaca tctatatgaa agaagtagat ggtaagttag tagaaggtag tccagatgat
1561 tacactgata taaaatttag tgttaaatgt gcgggaatga ctgacaagat taagaaagag
1621 gttacgtttg agaatttcaa agtcggattc agtcggaaaa tgaagcctaa gcctgtgcaa
1681 gtgccgggcg gggtggttct ggttgatgac acattcacaa tcaaataa
//