GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS SP2SP02L 3099 bp ds-DNA PHG 25-MAY-1991
DEFINITION Bacillus subtilis phage SP02 gene L for DNA polymerase
ACCESSION X01458
KEYWORDS overlapping genes; polymerase; unidentified reading frame.
SOURCE Bacteriophage SPO2 DNA.
ORGANISM Bacteriophage SPO2
Viridae; ds-DNA nonenveloped viruses; Siphoviridae.
REFERENCE 1 (bases 1 to 3040)
AUTHORS Raden,B. and Rutberg,L.
TITLE Nucleotide sequence of the temperate Bacillus subtilis
bacteriophage SP02 DNA polymerase gene L
JOURNAL J. Virol. 52, 9-15 (1984)
STANDARD full automatic
REFERENCE 2 (bases 1 to 3099)
AUTHORS Raden,B.
JOURNAL Unpublished (1985)
STANDARD full automatic
COMMENT SWISS-PROT; P06225; DPOL$BPSP2.
Data kindly reviewed (11-SEP-1985) by Raden B.
From EMBL entry STSP02L; dated 06-JUL-1989.
FEATURES Location/Qualifiers
misc_signal 27..48
/note="pot. transcriptional stop signal"
repeat_unit 27..32
/note="inverted repeat A"
repeat_unit 43..48
/note="inverted repeat A'"
promoter 137..142
/note="pot. -35 region"
promoter 159..164
/note="pot. -10 region"
RBS 346..351
/note="pot. ribosome binding site (ORF1)"
CDS 356..613
/note="ORF1 (aa 1-86)"
/codon_start=356
RBS 597..605
/note="pot. ribosome binding site (polymerase)"
CDS 613..2556
/note="SP02 DNA polymerase (aa 1-648)"
/codon_start=613
RBS 2564..2568
/note="pot. ribosoma binding site (ORF3)"
CDS 2577..2675
/note="ORF3"
/codon_start=2577
RBS 2662..2667
/note="pot. ribosome binding site (ORF4)"
CDS 2678..3064
/note="ORF4"
/codon_start=2678
BASE COUNT 976 a 557 c 779 g 787 t
ORIGIN
1 aagcttttta gattaaaaga actatgaggg gagacgagag tttccccttt ttaatagatt
61 gggagtgaaa acatgtttaa tccatcagac tggtatatca cacctcaaga atacgaacgg
121 gcagctaaaa acggtattga caaaatacgc cttgaaagaa gaatacgttt ctcaggatgg
181 gataaagaaa gggccataac aacaccagta aaaaaacgca agccaaagtc aaaatggggc
241 tgatgttgca gtatcaaacg gaattcaccg aaacttattt tttgcccgtg tgaataggct
301 gggctgggat gaagaaaagg ccgcgacaac cccggcatct gacgtgaggg agaacatgaa
361 aaaagtacaa aagcattcgt ctcgtaataa acactatcgt cattcccctg agttgatagc
421 tcttgctgag tcgaacggca ttaaatacct gacctatatt aaccgggtca ataactgcgg
481 aatggaccct tacgaagctg cagttacacc ccttagatca cgtcaagagg ttgcccaact
541 cggccgtaaa gctttccaag aaaaatacgg ggatgtaaat gggctatttt ttcaaaaaag
601 gcgggcttct aattgaaaac tttatcaatt gatatagaaa cattctcaag cgttgatctt
661 ttaaaagcag gcgtttacgc gtacacagaa gcccctgact ttgaaatttt gttgtttgct
721 tatgctttcg atgatgatcc tgtaaaaata attgatctgg ctcaggggga tacgctaccg
781 catgaggtgc ttgtggcgct gacaagttcg aaggtaatca agacggcata taacgctaac
841 tttgaaagaa cgtgtattgc aaagcatttt aatttaatgc tgcttccggc gcagtggagg
901 tgtacagctg tccacgctac tactttaggg cttcctggga atcttgacgg agtggctaaa
961 gcgttaaagc tatcagcgca gaaggataaa gcaggtaaag cccttattcg ttacttttca
1021 gtaccgtgta aaccaaccaa ggcgaatggg caaagggtta ggaatctacc cgagcacgac
1081 ccggagaaat gggagaaatt caaagtctat tgtatccagg atgttgaagt agaacgtgca
1141 atcaaaaacc gcatttccaa gtttgaacct ttggagtctg aacataaatt atgggcgtta
1201 gatcaggaaa taaatgatag gggtgttcgg atagatgtgg acctagtcaa gcatgcaata
1261 gcctgtgatg aacaatatca ggccggctta atagcagagg ctaagaagtt gaccggtctg
1321 cccaatccca acagtacggc gcaattaaaa aaatggcttg aagagaaggg gcttactatt
1381 tccagtctag ctaaagacaa aatagaggaa cttattgaaa acacaaacga tgaaactgtt
1441 cacagggttt tgagattaag gcaagaaatg gcgaaaacct cagttaaaaa gtatctggca
1501 atggagaaag ctctttgccc tgataatcgt gttcgcggct tacttcaatt ttacggtgcc
1561 agccgtactg gcagatgggc cggacgtttg gttcaagttc agaatttgcc acaaaacaag
1621 atagaggacc tggacacagc caggaactta ttgaagggcg gacactatga agcgatcgaa
1681 ttgctttatg gccaagttcc ttttgtactg tctcagcttg ttagaacagc ttttatcccg
1741 tcagaaggta atgagtttta tgtctctgac ttctcggcca tcgaggcccg tgtaattgct
1801 tggcttgcag gagaagaatg gcgtttggaa gtgttcaaca ctcacgggaa gatttacgag
1861 gcttcggcag cgcaaatgtt caaggttccg gttgagtcaa tcactaaagg cagcccatta
1921 agacaaaaag gtaaagttgc cgagttagct ttgggctacc agggcggaaa aggtgcgctt
1981 attcagatgg gtgccctaaa catgggctta gcagagggtg aacttcctga gcttgtaaaa
2041 gcttggcgga cagcaaacaa aaagatagtt aagttttggt atgacgtaga agccgcagca
2101 attaaagcag ttaaagagag aaagccagtt aagcttcagc acggcctaac cttcttatac
2161 gaatctggca ttttgtttgt acagctaccg tcaggcaggc gcctggcata tgcgaagcca
2221 aaacttgaat tggatgaacg ctttggaaaa gaagcactta cttatgaggg gaaacttgag
2281 tctggtaagt gggggcggtt gaacacatac ggcggcaagc ttgttgagaa tatcgttcag
2341 gcaactgcac gggattgtct ggctattaca cttatgcgtt tagacaatgc tggctataaa
2401 actgtcatgc acgttcatga tgaagctgtc ctggatgttc cccgagggaa aaatgaattg
2461 gataaagtag aggccattat gggagagcct atttcttggg ctaagggtct acccttgacc
2521 gcggacggct ttgttacaga ttactataaa aaagattaat agtaggggct gagattatga
2581 gttttataag aagtctaata gccgtgttcg ggcttttatt ctcagtgagg aaaaaggaag
2641 tcgaatatta cgaatggata gaaagggatg gtaagtgatg gctattatta aaaattattc
2701 acttggggat tttggctgca ttgggaccga aaccgagttt attttgatca tcaaaaataa
2761 aatttatggg ccttggggta atgagcgctt tgagtccgtt ttaaatactt tagaaggcgt
2821 acttgagaag cctcgaaaat caaaaagatc gggggcgcaa atgtttatga gtgatgataa
2881 ggaactggct cgattaaaaa gaaaggtcga tagactcatt gaaaaatgcg atagaaaagg
2941 gcatgagttt aatgattttg aaatcgggac cattaggaga atagggcacg ccaaaagcat
3001 ggatgatctc aactatttgg taggaatttc aatacaaatg atttttgatg aacacaagat
3061 cagataagga ggacaggagt gacacaagaa gaattgcat
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