GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS CRECR16S 3218 bp ds-DNA ORG 25-MAY-1991
DEFINITION C. reinhardii chloroplast gene for 16S ribosomal RNA
ACCESSION X03269 J01395
KEYWORDS 16S ribosomal RNA; direct repeat; inverted repeat; ribosomal DNA;
unidentified reading frame.
SOURCE Chloroplast Chlamydomonas reinhardtii DNA.
ORGANISM Chloroplast Chlamydomonas reinhardtii
Eukaryota; Plantae; Thallobionta; Chlorophycota; Chlorophyceae;
Volvocales; Chlamydomonadaceae.
REFERENCE 1 (bases 1233 to 3218)
AUTHORS Dron,M., Rahire,M. and Rochaix,J.D.
TITLE Sequence of the chloroplast 16s rrna gene and its surrounding
regions of chlamydomonas reinhardii
JOURNAL Nucleic Acids Res. 10, 7609-7620 (1982)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1232)
AUTHORS Schneider,M., Darlix,J.-L., Erickson,J. and Rochaix,J.-D.
TITLE Sequence organization of repetitive elements in the flanking
regions of the chloroplast ribosomal unit of Chlamydomonas
reinhardii
JOURNAL Nucleic Acids Res. 13, 8531-8541 (1985)
STANDARD full automatic
REFERENCE 3 (sites)
AUTHORS Harris,E.H., Burkhart,B.D., Gillham,N.W. and Boynton,J.E.
TITLE Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA
genes of Chlamydomonas reinhatdtii: correlation of genetic and
physical maps of the chloroplast genome
JOURNAL Genetics 123, 281-292 (1989)
STANDARD full staff_review
COMMENT SWISS-PROT; P05722; YCS2$CHLRE. SWISS-PROT; P05723; YCS1$CHLRE.
SWISS-PROT; P05724; YCS3$CHLRE.
From EMBL entry CHCR16S; dated 11-JAN-1990.
FEATURES Location/Qualifiers
repeat_region 30..43
/note="direct repeat 1"
repeat_unit 32..54
/note="inverted repeat a"
CDS complement(115..465)
/note="pot. unidentified reading frame 2"
/codon_start=465
CDS 152..484
/note="pot. unidentified reading frame 1"
/codon_start=152
repeat_unit 198..210
/note="inverted repeat a'"
CDS 519..890
/note="unidentified reading frame 3"
/codon_start=519
misc_feature 810..1279
/note="pot. stemloop structure"
repeat_unit 810..827
/note="inverted repeat b"
repeat_unit 843..861
/note="inverted repeat c"
repeat_unit 905..935
/note="inverted repeat d"
repeat_unit 930..968
/note="inverted repeat e"
repeat_unit 1026..1043
/note="inverted repeat f"
repeat_unit 1037..1056
/note="inverted repeat c'"
repeat_unit 1070..1081
/note="inverted repeat g"
repeat_unit 1083..1106
/note="inverted repeat f'"
repeat_unit 1085..1105
/note="inverted repeat h"
repeat_unit 1148..1164
/note="inverted repeat i"
repeat_unit 1165..1175
/note="inverted repeat g'"
repeat_unit 1174..1194
/note="inverted repeat h'"
repeat_unit 1215..1245
/note="inverted repeat d'"
repeat_unit 1247..1263
/note="inverted repeat i'"
repeat_unit 1260..1279
/note="inverted repeat b'"
repeat_region 1329..1346
/note="direct repeat 2"
promoter 1345..1350
/note="put. -35 region"
repeat_region 1350..1368
/note="direct repeat 2"
repeat_region 1358..1371
/note="direct repeat 1"
promoter 1368..1373
/note="put. -10 region"
misc_feature 1378..1379
/note="16S rRNA transcription initiation region"
rRNA 1455..1947
/note="16S ribosomal RNA"
mutation 1468..1468
/note="t is g in mutant sr-u-sm3"
mutation 1929..1929
/note="a is c in mutant sr-u-2-60"
mutation 2311..2311
/note="c is t in mutant sr-u-2-23"
mutation 2313..2313
/note="a is c in mutant sr-u-sm5"
mutation 2314..2314
/note="a is g in mutant sr-u-sm-3a"
mutation 2588..2588
/note="a is g in mutant spr-u-1-6-2"
mutation 2588..2588
/note="a is g in mutant spr-u-1-H-4"
mutation 2588..2588
/note="a is c in mutant spr-u-sp-23"
mutation 2590..2590
/note="g is a in mutant spr-u-1-27-3"
mutation 2810..2810
/note="a is g in mutant nr-u-2-1"
mutation 2811..2811
/note="c is t in mutant kr-u-24-2"
BASE COUNT 981 a 658 c 745 g 816 t 18 others
ORIGIN
1 ggatcctaac aaaatgttgg ctgtctggta taaaaaagta aaagaaacta acaattttag
61 ttgtttatat gatgatattg atcgagcatc gtgagatact ccatcagagg atcatctact
121 atcgtttaac catgaacgag ctttgcgaga tatgatatgc ttcaaacaac aaaaaattac
181 tagcttattt gtttcgctta ttttactttt aacaacgatt ttaatttgtt ggttttgtgt
241 tttaagtcct aaggtaaacg gtaccaatag tccaattgaa gtaaatgtat ataatccaat
301 tccattggct gtaatgagag gaggtattcc tctaccaggt atgcctccag ttcctaccgc
361 aacaccttca ttacctcgct cggggtttac gtcgtcagct aaaaaaatta aagaatcgcg
421 taaacaaaaa agcactgcac tacaagcggt taaagaccaa tacattttac gcgtggcgcg
481 attatgattg tacaaagtga cccaaagggt ttaattttgt gcagggttta gaatataccc
541 tcgaacccga gcaagctaaa aaatgcaaaa ctcatcaact tgttgactta gcgaaaatcg
601 atttaaatag ctggtcacaa gaaaatgtaa gacctaaaac cttgttaggt cgaacgtcaa
661 aaaaacgttt aacaagtcaa ttttggtacg agtttactaa aaaacaaaaa ctgaaaaacg
721 ctctttatta ttttgtcatt tatgacatca cagacaattc tatactgccg gtagagacac
781 taaaccaaaa aaaccctcta tcaacctcct tccccttccc cttcgggcgt ccccttcgtg
841 ggataaattt tagtggcagt tgcctgcact gcctccttcg agtgttaaaa tagacgacag
901 tgcagttgcc tgccaactgc ctatatttat atactgcgat aaactttagt cccgaagggg
961 tttacatatc cgaaggagga agcaggcagt ggcggtacca cgccactggc gtcctaatat
1021 aaatattggg caagtaaact tagaataaaa tttatttgct gcgttagcag gtttacatac
1081 tcctaagttt acttgcccga aggggaagga ggacgtcccc tacgggaata taaatattag
1141 tggcagtggt acaataaata aattgtatgt aaaccccttc gggcaactaa agtttatcgc
1201 agtattaaca tcctagtata taaatatcgg cagttggcag gcaacaaatt tatttattgt
1261 cccgtaaggg gaaggggaaa acaattatta ttttactgcg gagcagcttg ttattagaaa
1321 tttttattaa aaaaaaaata aaaatttgac aaaaaaaaat aaaaaagtta aattaaaaac
1381 actgggaatg ttctaacaat cataaaaaaa tcaaaagggt ttaaaatccc gacaaaattt
1441 aaactttaaa gagtatccat ggagagtttg atcctggctc aggacgaacg ctggcggcat
1501 gcttaacaca tgcaagtcga acgagcaaag caatttgtgt agtggcgaac gggtgcgtaa
1561 cgcgtaagaa cctacctatc ggagggggat aacattggga aactgttgct aataccccat
1621 acagctgagg agtgaaaggt gaaaaaccgc cngatagagg ggcttgcgtc tgattagcta
1681 gttggtgggg gtaacggcct cccaaggcca cgagcagtag ctggtctgag aggatgatca
1741 gccacactgg gactgagaca cggcccagac tcctacggga ggcagcagtg aggaattttt
1801 cgcaatgggc gcaagcgacg gagcaatgcc gcgtgcagga agaaggcctg tgggtcgtaa
1861 actgcttttc tcagagaaga agttctgacg gtatctgagg aataagcacc ggctaactct
1921 gtgccagcag ccgcggtaat acagagggtg caagcgttgt ccgcaatgat tgggcgtaaa
1981 gcgtctgtag gtggctcgta aagtctaatg tcaaatacca gggctcaacc ttggaccggc
2041 attggagtac tcacgagctt gagtacggta ggggcagagg gaattccatg tggagcggtg
2101 aaatgcgtag agatatggag gaacaccagt ggcgaaggcg ctctgctggg ccgaaactga
2161 cactgagaga cgaaagctgg gggagcgaat aggattagat accctagtag tcccagccgt
2221 aaactatgga gactaagtgc tgccgcaagc agtgctgtag ctaacgcgtt aagtctcccg
2281 cctggggagt atgctcgcaa gagtgaaact caaaggaatt gacgggaccg cacaagcggt
2341 ggattatgtg gattaattcg atacaacgcg aagaacctta ccagggtttg acatgtcaag
2401 aancctctca gaaatgggag ggtgccctaa cggacttgaa cacaggtggt gcatggctgt
2461 cgtcagctcg tgctgtgaag tgtatagtta agtctcataa cgagcgcaac cctcgtcttt
2521 agttgccatt nnnnnnnntg gttctctaaa gagactgcca gtgtnaagct ggaggaaggt
2581 gaggatgacg tcaagtcagc atgcccctta catcctgggc ttcacacgta atacaatggt
2641 tgggacaatc agaagcganc tcgtgagnnn nagctagcgg ctctgttaaa cccaacctca
2701 gttcggattg taggctgcaa ctcgcctaca tgaagccgga atcgctagta atcgccagtc
2761 agctatatgg cggtgaatac gttcccgggt cttgtacaca ccgcccgtca caccatggaa
2821 gctggttctg ctccaagtcg ttaccctaac cttcgggagg ggggcnngcc taaagcaggg
2881 ctagtgacta gggtgaagtc gtaacaaggt agggctactg gaaggtggcc ctggctcacc
2941 tccttcattt ttaaccctta tgggtatata ataagagttt tagctataaa actcaactaa
3001 aggtaacgtg gttgaattcc cacgttacct tttaaacctc attataaaaa tttataatat
3061 ataaatatat taatttaata attaattacc ctccttcgct aacccctaac gggcaataaa
3121 taaatttgtc cccttcccct tacggcaata aataaatttg ttgcctgcca actgcctcct
3181 tcgggattta aaacctatat ttatataact ccgaagcg
//