GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS TOBNTARG 1076 bp ds-DNA ORG 25-MAY-1991
DEFINITION Tobacco chloroplast genes for tRNA-Arg (ACG) and tRNA-Asn (GUU)
ACCESSION X02441 V00164
KEYWORDS direct repeat; inverted repeat; transfer RNA; transfer RNA-Arg;
transfer RNA-Asn; unidentified reading frame.
SOURCE Chloroplast Nicotiana tabacum DNA.
ORGANISM Chloroplast Nicotiana tabacum
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Asteridae; Solanales; Solanaceae.
REFERENCE 1 (sites)
AUTHORS Kato,A., Shimada,H., Kusuda,M. and Sugiura,M.
TITLE The nucleotide sequences of two tRNA-Asn genes from tobacco
chloroplasts
JOURNAL Nucleic Acids Res. 9, 5601-5607 (1981)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 1076)
AUTHORS Kato,A., Takaiwa,F., Shinozaki,K. and Sugiura,M.
TITLE Location and nucleotide sequence of the genes for tobacco
chloroplast tRNA-Arg(ACG) and tRNA-Leu(UAG)
JOURNAL Curr. Genet. 9, 405-409 (1985)
STANDARD full automatic
COMMENT Data kindly reviewed (15-NOV-1985) by M. Sugiura
From EMBL entry CHNTARG; dated 06-JUL-1989.
FEATURES Location/Qualifiers
promoter 16..21
/note="pot. -35 like region"
promoter 41..47
/note="pot. -10 like region"
RBS 138..141
/note="pot. ribosome binding site"
CDS 147..272
/note="unidentified reading frame ORF 42"
/codon_start=147
tRNA 150..223
/note="tRNA-Arg (ACG)"
repeat_region 219..226
/note="imp. direct repeat"
repeat_region 230..237
/note="imp. direct repeat"
misc_feature complement(250..251)
/note="tRNA-Arg-like sequence"
RBS 263..266
/note="pot. ribosome binding site"
CDS 272..385
/note="unidentified reading frame ORF 38"
/codon_start=272
misc_signal 389..405
/note="pot. regulation signal, stem loop structure"
repeat_unit 389..393
/note="inverted repeat A"
repeat_unit 401..405
/note="inverted repeat A'"
misc_feature 728..743
/note="pot. stem loop structure"
repeat_unit 728..734
/note="inverted repeat B"
repeat_unit 737..743
/note="inverted repeat B'"
misc_feature 751..769
/note="pot. stem loop structure"
repeat_unit 751..756
/note="inverted repeat C"
repeat_unit 764..769
/note="inverted repeat C'"
CDS complement(773..997)
/note="unidentified reading frame ORF 75"
/codon_start=997
tRNA complement(805..876)
/note="tRNA-Asn (GUU)"
RBS complement(1008..1012)
/note="pot. ribosome binding site"
promoter complement(1028..1034)
/note="pot. -10 like region"
promoter complement(1049..1051)
/note="pot. -35 like region"
BASE COUNT 284 a 261 c 237 g 294 t
ORIGIN
1 tcccttctct cccacttcac acctcggaac gcacccttct tatagagata aacgcgcctt
61 cacatcttct taacccgaaa tggctgggga gaggaaaggt tccttttttt gagggtactc
121 ccgggaacag atccagtgga gacggggtgg ggcctgtagc tcagaggatt agagcacgtg
181 gctacgaacc acggtgtcgg gggttcgaat ccctcctcgc ccacaaccgg cccaaaaggg
241 aagtaccttt ccctctgggg gtaggaaaat catgatcggg atagcgaacc aaaagctatg
301 gaacttgggt gtgggtcttt tgtcgaaatg gaatggcttt tctttttctc tttttattta
361 tcgtgaatgg gggaatcatt acacatagta tgcccggtca gcatattttt ttgttttacg
421 ccccgtaact cttcctcagc caggcttggg cagaatagca gagcaagtat tagtagcata
481 acaaaaaagc cttcctcgtc attaatatct ttgctcgcgg caattgtgac ctctcgggag
541 aatcgatgac tgcatctttg atgcagtgct agtatatctg agacttctta attggctagt
601 tgtaaatagc cccagggcta tggaacaaag gattatctcg gacctagacc gaggtattga
661 tggtgatttt ctaatctcgc agaacagaat gtgatacgat gagatagaat gcaatagaaa
721 caaagacagg gaacgggtta cctactctta acgggcaaag cgagcccctt tattctgaat
781 tctttaattc agaatcaatc aaatctcccc aagtaggatt cgaacctacg accaatcggt
841 taacagccga ccgctctacc actgagctac tgaggaacaa caggagattc gatctcatag
901 agttcaattc ccgttcccaa cccatgacca atatgagctc gaagcttcct tcgtaactcc
961 cggaacttct tcgtagtggc tcccttacat gcctcatttc agagggaacc tcaaagtggc
1021 tctatttcat tatattccat ccatatccca attccattca tttaatatcc ctttgg
//