GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS MUSDHFRV 671 bp ss-mRNA ORG 25-MAY-1991
DEFINITION Mouse mRNA for dihydrofolate reductase variant
ACCESSION X56066
KEYWORDS DHFR gene; dihydrofolate reductase.
SOURCE Mitochondrion Mus musculus RNA.
ORGANISM Mitochondrion Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 671)
AUTHORS Simonsen,C.C.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS McIvor,R.S. and Simonsen,C.C.
TITLE Isolation and characterization of a variant dihydrofolate reductase
cDNA from methotrexate-resistant murine L5178Y cells
JOURNAL Nucleic Acids Res. 18, 7025-7032 (1990)
STANDARD full staff_review
COMMENT *source: cell type=lymphoma;
Data kindly reviewed (15-JAN-1991) by Simonsen C.C.
From EMBL entry MMDHFRV; dated 16-JAN-1991.
FEATURES Location/Qualifiers
mutation replace(:117..118,:117..118)
/phenotype="wildtype"
/product="dihydrofolate reductase"
/gene="DHFR"
/citation=[2]
5'UTR 7..22
mat_peptide 23..583
/note="variant"
/product="dihydrofolate reductase"
/gene="DHFR"
CDS 23..586
/note="variant"
/product="dihydrofolate reductase"
/gene="DHFR"
/codon_start=23
3'UTR 584..671
BASE COUNT 203 a 145 c 154 g 169 t
ORIGIN
1 aagctttatc cccgctgcca tcatggttcg accattgaac tgcatcgtcg ccgtgtccca
61 agatatgggg attggcaaga acggagacct accctggcct ccgctcagga acgagtggaa
121 gtacttccaa agaatgacca caacctcttc agtggaaggt aaacagaatc tggtgattat
181 gggtaggaaa acctggttct ccattcctga gaagaatcga cctttaaagg acagaattaa
241 tatagttctc agtagagaac tcaaagaacc accacgagga gctcattttc ttgccaaaag
301 tttggatgat gccttaagac ttattgaaca accggaattg gcaagtaaag tagacatggt
361 ttggatagtc ggaggcagtt ctgtttacca ggaagccatg aatcaaccag gccacctcag
421 actctttgtg acaaggatca tgcaggaatt tgaaagtgac acgtttttcc cagaaattga
481 tttggggaaa tataaacttc tcccagaata cccaggcgtc ctctctgagg tccaggagga
541 aaaaggcatc aagtataagt ttgaagtcta cgagaagaaa gactaacagg aagatgcttt
601 caagttctct gctcccctcc taaagctatg catttttata agaccatggg acttttgctg
661 gctttagatc t
//