GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS BNABP4 8585 bp ds-DNA PLN 25-MAY-1991
DEFINITION Brassica napus Bp4A and Bp4C genes expressed in early microspore
development
ACCESSION X52874
KEYWORDS .
SOURCE Brassica napus DNA.
ORGANISM Brassica napus
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Dilleniidae; Capparales; Brassicaceae.
REFERENCE 1 (bases 1 to 8585)
AUTHORS Fabijanski,S.F.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Albani,D., Robert,L.S., Donaldson,P.A., Altosaar,I., Arnison,P.G.
and Fabijanski,S.F.
TITLE Characterization of a pollen-specific gene family from Brassica
napus which is activated during early microspore development
JOURNAL Plant Mol. Biol. 15, 605-622 (1990)
STANDARD full staff_review
COMMENT *source: strain=westar; library=charon 4A; clone=Bp4;
From EMBL entry BNBP4; dated 17-JAN-1991.
FEATURES Location/Qualifiers
mat_peptide join(308..368,1134..1261)
/product="Bp4A product"
/gene="Bp4A"
mat_peptide join(6369..6429,7196..7353)
/product="Bp4C product"
/gene="Bp4C"
mRNA join(236..368,1134..1427)
/gene="Bp4A"
/evidence=EXPERIMENTAL
mRNA join(6297..6429,7196..7489)
/gene="Bp4C"
/evidence=EXPERIMENTAL
CDS join(308..368,1134..1264)
/product="Bp4A product"
/gene="Bp4A"
/codon_start=308
CDS join(6369..6429,7196..7356)
/product="Bp4C product"
/gene="Bp4C"
/codon_start=6369
TATA_signal 84..91
exon 236..368
/number=1
prim_transcript 236..1427
/gene="Bp4A"
exon 236..368
intron 369..1133
/number=1
exon 1134..1427
/number=2
exon 1134..1427
polyA_signal 1402..1407
TATA_signal 3052..5059
/note="tata signal for Bp4B gene"
misc_feature 3133..4109
/pseudo
/note="nonfunctional Bp4B gene"
TATA_signal 6266..6273
exon 6297..6429
/number=1
prim_transcript 6297..7489
/gene="Bp4C"
exon 6297..6429
intron 6430..7195
/number=1
exon 7196..7489
/number=2
exon 7196..7489
polyA_signal 7464..7469
BASE COUNT 3148 a 1238 c 1188 g 3011 t
ORIGIN
1 gaattctaaa aatagcaata actttttgag aacatcagat ttatgtacac gcataggaca
61 catacctttt tatttactta aaggaaaatg aacgagtcta aatcttccac atgttatatg
121 agcaaaacat gaatttttct aaattagatt cgtttaaatc agaacatatt aatgtgagtt
181 tcttaaatta gatttttaat atctatatat acgtaagaat acttcttatg ttttaaaata
241 aaaaatagaa tacttcatct ctttcctaaa tttttaagcc aatatcaatc catttctata
301 atctaagatg aagaaatccc ttcaactctc tttttcgttc ttaattatct ccatcattct
361 ctcacatggt ttgtattttc atcttaatat attgcatata gtaattccat aataaattga
421 ttatactaaa atttgacttt taaaatattg tcaaccccca tataataaat tttatttact
481 atataaaaca tagcattaaa ttatctcttt gtgtaaaatt cataactttg cagaaggcta
541 gaaaatatag atagtatagt cagaaatgtt tgcgttaaaa ttgaaaggat caaccatgga
601 gtatttaaat gttttttata ctttatgcca tttataattt ttttaatgta tgggtttata
661 tatgatgaag aactattatg ataaaataat attaaataat ttcattttta tcatctattt
721 atgaacattt tgtcctgcac atacaaatga tttaaccaac atttttaata atatggatga
781 actatagctc ttacgtaaat ttatttgata tttttaatta aatttatata tttttatagg
841 taatttgtta tgcttttcca atacatacag tagttgttat taaaatatca aaatttaata
901 cgtaatgttt attaatatgc acacaattct taaaaccata tttttaaaaa ataatgtgtg
961 accaaacgat atgctcattt ttttatttac tagcaaaata tatttctttt cttacttata
1021 acgtttaaaa agaaatgtta ttaaacattt tttgctgata aataaatttt atatttcata
1081 aaatctaaat atatttttta acaattaaaa tttgaaattt ttatatctta caggaatgat
1141 ggcagatgcg cagaaaaaga attgtcctca taaaattcca ataaaaggaa gttattgtgc
1201 tccaactata tgtttggata tgtgtaagaa gcaacatgga actgttggta gttgtgcgga
1261 ataaaaatga ttttgtaact gcgcttgtaa gtaagggttc tcactaagtg ttatgaatct
1321 agtaatgtcc aaccaaagtt ttatattatt tcttttaaca ataagtctaa atgtttgtct
1381 cagatttgtg gatctattta taataaataa taatatgaat gttaaataaa tacaaatgtg
1441 taaaacaaga gtggactatt aataaaatat atgattacat tattgttaga agtaaccaat
1501 attacgtgta aaatcaaaat cttaagacaa gttaaaaaga ttgagatgaa atcacaacca
1561 atatttaaat gtgagataat caactaacat gtaattttgt acacattgta aaaaaaaaaa
1621 agcaagagtt cattatcaaa caagaaagtg ttagaaagag caacagattc attgcaaggg
1681 cagtctaggt tgaattggct tgacataggg aaaattgaaa gcactgtttc tgaacatgac
1741 aacgcttggt caggaagaac aatctcacaa ccagagtttt gggtagattt ctccaatgtc
1801 attatcaggt acgagttatg agacttcatc cacatctcag tcccagttcc cttctcagga
1861 agtttccttg aggaaggagg ttattacaga aagctaagtt acatgagcct gacatatcat
1921 gcaagggcag tcccaacaag aaaatgttag aaagagcaac atatcatgca aggacagtcc
1981 aggtttgaat tggcttgaca gatggtttgc agacatgcca tctgaaggtc ctacaaactc
2041 atcagacaac gaaggaaaat tgatagcatt gtttctgaac atgacaaaac tctagtcagg
2101 aagaagaatc tcacaaccaa agttttgggt agagctcctc caatgtcatc atcagctacg
2161 agttcaaaga cttcatccac atcttagtcc ccattctctt ctcatgaagt ttcctttagg
2221 aaagaggtta ttacagaaag ccaagttaca tgagcctaac acaatctatc aaagctaaga
2281 agagacgatt aaccattatt cttcatcttt ttgtcccaaa atcactgttt gaaaagaaac
2341 agtccatgtc ttacaacgaa gatgtgaatg taaagcgttg tgcatgttcg gatccatctt
2401 gcaaccagca gagttatcta tatccacatg ctcaagtaac aaggaaaaat atgtgggcaa
2461 agaaggtaaa gtgaacatcg tttcattaat tcattaaagc atttcaacac cttgatggtt
2521 ctaaatacac acaaaacact gatttataga tacataagca acttctgtgt atgttctttt
2581 acatacaagt tggtatcaag gctgttgcaa tgttgttgtt tgaccacttt tattatttaa
2641 tagttaactt ttgatgcttc taagataata tgttcctcct aactcttgtc aacatgaaag
2701 accaattaaa ggttttgatt aaatacatac taatttttta atataatctt ataagttatg
2761 ttacgtgtgg gacatccacc taataaacta taaatttaaa taataatgtt tgaaaaggat
2821 tttattgaca ttcctttaaa taaattcata attttaaaaa tagcgataac tttttgaaaa
2881 catcagaatt atgtacacgc agaggacaca taccttttta tttacttata ggaaaatgaa
2941 cgagtctaaa gcttccacat gttatatgag caaaacatgg atttttctaa attagattcg
3001 tttaaatcag aacatattaa tgtaagtttc ttaaattaga tttttaatat gtatatatat
3061 gtaaaaatac ttcttttttt tttttttgtc atcagcatta cagatttcta aataagttac
3121 ttcttatgtt ttaacaaata gaatacttca tctctttcct aaattttaag tcaatatcaa
3181 tccatttcta taatccgaag atgaagaaat cccttcatct ctagaaaata gggtcagaaa
3241 gttttgcgtt aaaattgaaa ggatcatccc tgaagtattt atttgttttt tttatgcttt
3301 agtccattaa tattttttta atgtatgggt ttatatatga ttaagaactt ccatgataaa
3361 ataatattaa atagttttat ttcttatcat ctatttatga acgtttgttc ctgcacacac
3421 aaatgattta accaacattt ttcataatat ggataaacta tagttcttat gtaaatttat
3481 gtgatatttt aattagattt atatatttat aggtaatcta ttatgctttt ccaatacata
3541 cagtagttgt tcttaaaaca tcaaattttt atatgtaatg tttattaata tgcacacaat
3601 tcttaaaaca atattttcac aacataaaaa aataatgttt gaccaaacca tatgctcatt
3661 ttctttattt accggcaaaa accatttctc cattttttta cttataacgc ttaagataaa
3721 aaaattatta aacagttttt gttgataaat aagttttata tttcagaaaa tgtattatat
3781 tttcaaacaa ttaaaatttg ggtttttata tctaacataa atgatggcag aagcacagaa
3841 aaataattgt cttcataaaa ttccaataaa aggaagctag tgcattccaa ataaatgttt
3901 ggctatgtgt aagaagcaac atggaactct tggtagttgt ccggaaaaag aaatttgtag
3961 ttgtgcttgt aagtaagggt tctcactaag tgttatgaat ctaataatgt ccaaccaaag
4021 ttgtatataa ttttttaaca ataaatgtct aaatgtttgt ctcagatttg tggatctatt
4081 tataataaat aataatatga atgttaaata aatacaaatg tgtaaaaaaa gattggacta
4141 ttaataaaat aaatgatcac attattatta gatgtaacca atattgtgta taagatcgta
4201 aaagcttaag acgagttaaa aagatagaga tgaaatcaca tccaatatct aaatgtgaga
4261 taatcaacta acatataatt ttgtatatat tgtaagataa aataaaaata aaaattaaaa
4321 agcaagagtt gattatcaaa caaagaagta ttagaaagag caacagatca tgcaagaaga
4381 gtccatgttt gaattttctt gacagatggg ttgcagacaa gtcatgggaa ggtcatacaa
4441 actcatcaga caacgcaaag aaaattgata gcacagtttc tgaacatgac aaagctctgg
4501 tcatgaagaa caatctcaca agcagagttt tgggtagacc tcctccaatg tcatcatcag
4561 ctacgagctc tgagacttca tccacatctc agtcctcagt ttcttcccag gaagtttcct
4621 tgaggaagga ggttattaca gaaatccaag ttacatgagc ctggcaaaat ctatcaaagc
4681 taagcagaga tgatcacggt attcttcatc gtcttcttcc aaaacctggt ttgagaaaaa
4741 acaatccatg tcttacaaca gagatgtaaa tgtaaagtgt tgtgtttgtt cggatcaatc
4801 ttgcaaccag tggagtgatc tatatcgacc ggttcaagta acaaggagaa ctatgtggga
4861 aagaggctaa attaaacatc gtttcatcaa agattgttgc aatgttgttg tttggccact
4921 ttgattattt aataattaac ttcggatgct tctgagacaa tctgttcctc ccattttttg
4981 tcaatatgaa acgaagagca atgcttcatc tttagacatg aaaagccatt taaatgacca
5041 aataacatag tttataccaa agcttcctta taaattttac ccgttctaaa aattgctctt
5101 actatcaaaa tctaaaactg aattaaattc aattatctta ctgttacaca gttttcacta
5161 atcactattt taatgtataa actataaaaa taaattaaat acttactaaa tttttagatt
5221 taatccataa attatattac agttcagaga tttcatccac atttcagtcc ccagtccctt
5281 actcattaaa ttttcctgag gaaggaggtt atacagaaag tcaacttaca tgagccttac
5341 tcaatctatc aaagctaaga agagatgtca ggttcttctt catctttctg ttccacatca
5401 ccatttaagt agaaacagta tatgtcttac aacggtgatg tgaatgtaaa gggttgtgct
5461 ggttcggagt agactgatct atatccacca gtgcaaggaa catggaggca tatgtgggct
5521 aaaaaaaaca tcattaactg aatctttaaa gcactttcaa catcttgttg gttccatgaa
5581 atacataaat ggattatagg ttattgaagc cattgttgta tatgtttctt acttataaat
5641 tagtttgaag acagcaatgt tgttgtttgg ccactttgat tatttaataa ttaacttctg
5701 atctttctga gacaatatgt tcctcttatt tcttgtcaat atgaaaccaa gagcaaagtt
5761 tcatccttag acatgaaagg cttattaaat gaccaaataa catagtttag acgaaagctt
5821 cctaataaaa tttattctca ctatctaaat ctaaaactga attcaattca tctatctgat
5881 tattatatag ttttcatttt ttgttatttt attgaatgag taaaaaattt aattaaatac
5941 ttactatttt tttcatataa tcttataaat tatgttacgt gtgggacatc cacctaataa
6001 cctattaatt taaatagtaa tatttgaaaa atattttatt gacattgttt taaataaatt
6061 cataattcta aaaatagcaa taactttttg aaaacatcag atttatgtac acgcatagga
6121 cacatacctt tttatttact taaaggaaaa tgaacgagtc taaagcttcc acatgttatc
6181 tgagcaaaac atggattttt ctaaattaga ttcgtttaaa tcagaacata ttaatgtgag
6241 tttcttaaat tagattttta atatgtatat atacgtaaga atacttctta tgtttaaaaa
6301 aaaaaataga atactttatc tctttcctaa atttttaagc caatatcaat ccatttctat
6361 aatccaagat gaagaaatcc cttcaactct cgtttacgtt cttaattatc tccatcattc
6421 tctcacaagg tttgtattta catcttaata tattgcatat agtaattcca taataaattg
6481 attatactaa actttgactt ttaaaatatt gtaaaccccc catataataa attttattta
6541 ctatataaaa catagcatta aattatctct ttgtgtaaaa ttcataactt tgcagaaggg
6601 aagaaaatat agaaagtatg gtcagaaatg tttgcgttaa tattgaaaga atcaaccctg
6661 aagtatttaa ctgtttttta tactttatgc catttataat ttttttaatg tatgggttta
6721 tatatgatga agagctatta tgataaaata atattaaata gtttcatttt tatcatctat
6781 ttacgaacat tttgtcttgc acatacaaat gatttaaccg acatttttca taatatggat
6841 gaactatagt tcttacgtaa atttatttga tatttttaac taattttata tatttttatg
6901 cttttccaat acatacagta gttgttctta aaatatcaaa attttatacg taatgtttat
6961 taatatgcac acaattctta aaaccatatt tttcacaaaa taaaaaataa agtgtgacca
7021 aacgatatgc tcattttttt ttatatactg gcaaaatata tttctttttt tttttactta
7081 taacgtttaa aatgaaatgt tattaaacat tttttgctga taaataaatt ttctatttca
7141 taaaatctat atatattttc taacaattaa aatttgaaat tttaatatct tacaggaatg
7201 atggcagatg cgcagaaaaa gaattgtcct cgtaaaattc caataaaagg aagctattgt
7261 gctccaacta tatgtttgga taagtgtaag aagcaacatg gaactgttgg tagttgtgcg
7321 gaagaaaaag gattttgtaa ctgcgcttgt aagtaagagt tctcactaag tgtaatgaat
7381 ctagtaatgt ccaaccaaag ttttatatta tttcttttaa caataagtct aaatgtttgt
7441 ctcagatttg tggatctatt tataataaat attaatatga atgttaaata aatacaattg
7501 tataaaacaa gagtggacta ttaataaaat atatgatcac agtattgtta gaagtaacca
7561 atattacgtg taaaatcaaa agcttaagac tagttaaaaa tatagagatg aaatcacaac
7621 caatatttaa atgttatata atcaactaac atgtaatttt gtacacattg taaaaaaaaa
7681 aaaaaaaaaa aaaagcaaga gttgattaac aaacaagaaa gtgttagaaa gagcaacaga
7741 tcatgcaaga gcagtctagg tttgaattgg cttgacagat gtgttgcaga catgccatga
7801 ggaagtctta caaactcatc agacaacaca cagaaaattg atagcattgt ttctgaacat
7861 gacaaagctc tggtcatgaa gaaaaatttc acagccaaag tttttggtag accttctcca
7921 gctacgagtt ttgagacttc atccacatct cagtctccat ttccattctc atgaagtttt
7981 ctttagggaa gaggttatta cagaaagcca agttacatga acctaacaca atctatcaaa
8041 ggtaagaaga gacgatcaac cagtattctt catcttcttg ttccgaaatc actgtttgaa
8101 aagaaacagt caatgtctta caacgaagat gtgaatgtaa agtgttgtgc atgttcggat
8161 ccatcttgca cccagtggaa tgatctatat ctacatgctc aagtaacaag gagaaatatg
8221 tgggcaaaga agctaaagta aacattgttt cattaaatct ttaaagcatt tcaacacctt
8281 gagagttcta aaacacacac aaaataccga tttatagata tataagcaac ttctatgtat
8341 gttcttttac atacgagtta gtatgaagac tgctgcaatg ttgttgtttg accattttta
8401 ttatttaata gttaacttct gatgcttcta aaataatatg ttcttcccaa ctcttgtcaa
8461 tatgaaacca agagcaaagt tttaatttta gacatgaaac gcctattaaa tgaccaaata
8521 acatagttta gacgaaaact tcctaataaa atttattctc actatctaaa tctaaaactg
8581 aattc
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