GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS SOYRPB1B2 2822 bp ds-DNA PLN 25-MAY-1991
DEFINITION Soybean gene B2 for the largest subunit of RNA polymerase II RPB1
(EC 2.7.7.6)
ACCESSION X52493
KEYWORDS RNA polymerase; RNA polymerase II.
SOURCE Glycine max DNA.
ORGANISM Glycine max
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Rosidae; Rosales; Fabaceaea.
REFERENCE 1 (bases 1 to 2822)
AUTHORS Dietrich,M.A.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Dietrich,M.A., Prenger,J. and Guilfoyle,T.J.
TITLE Analysis of the genes encoding the largest subunit of RNA
polymerase II in Arabidopsis and soybean
JOURNAL Plant Mol. Biol. 15, 207-223 (1990)
STANDARD full staff_review
COMMENT *source: strain=var.Wayne; library=lambda gt10 cDNA; *source:
clone=Bprime clone; Includes PCR amplified genomic DNA. RPB1 is
encoded by a multigene family in soybean
From EMBL entry GMRPB1B2; dated 21-DEC-1990.
FEATURES Location/Qualifiers
mat_peptide join(12..1258,1522..1593,1762..1815,1917..2016)
/EC_number="2.7.7.6"
/note="largest subunit of RNA polymerase II"
/product="DNA-directed RNA polymerase"
/gene="RpII205 or gene B prime"
mRNA join(<1..1258,1522..1593,1762..1815,1917..>2019)
/gene="RpII205 or gene B prime"
exon <1..1258
/number=1
exon <1..1258
/note="reading frame 3"
CDS join(12..1258,1522..1593,1762..1815,1917..2019)
/EC_number="2.7.7.6"
/note="largest subunit of RNA polymerase II"
/product="DNA-directed RNA polymerase"
/gene="RpII205 or gene B prime"
/codon_start=12
intron 1259..1521
/number=1
exon 1522..1593
/number=2
exon 1522..1593
exon 1522..1593
exon 1522..1593
intron 1594..1761
/number=2
exon 1762..1815
/number=3
exon 1762..1815
exon 1762..1815
exon 1762..1815
intron 1816..1916
/number=3
exon 1917..>2019
/number=4
exon 1917..>2019
intron 2021..2441
/note="differentially spliced intron in 3'untranslated
region"
BASE COUNT 741 a 660 c 500 g 921 t
ORIGIN
1 ctctagctgt gatgtgccat gaagatgttg atgcgaccag gaccacaagc aaccacttga
61 ttgaagtcat tgaagttctt ggtattgagg cagttcggcg agctcttctg gatgaattgc
121 gtgttgtcat ttcttttgat ggttcttatg tcaattacag acatttggct attctttgtg
181 ataccatgac ttatcgaggg catttgatgg caattacacg tcatggtatc aaccgaaatg
241 atactgggcc aatgatgaga tgctcatttg aagaaacagt tgatattctg cttgatgccg
301 cagtatatgc tgagactgat tacttgaggg gagtcactga aaacataatg ttaggtcaac
361 ttgcccccat tggcactggc gaatgtgcct tgtatcttaa tgatgaaatg ctgaaaaatg
421 ccattgagct ccaattgcct agttatatgg atggtcttga ttttggtatg actcctgccc
481 gttccccaat atctggaact ccatatcatg aaggcttgat gtctcccagt tatttgttga
541 gcccaaattt acggctctct ccaactagcg atgcacagtt ttcaccttat gttggtggga
601 tggcattttc tcctacttca tctcctggtt acagcccatc atccccaggc tatagtccct
661 catcacccgg gtacagtccc acttcacccg gatacagccc cacgtcaccc gggtacagtc
721 ccacgtcacc tgggtacagt cccacctctc cgacttatag tcctagctct ccgggatata
781 gtcctacaag tccagcttat tcgcctacca gtccatccta ctctcccaca tcacccagct
841 acagtcctac atcacccagc tacagcccta catcacccag ctacagcccc acctctccca
901 gctacagccc cacttcacca gcttacagcc ccacttcacc agcttacagc cccacatctc
961 cttcctatag tccgacttct ccttcttata gtccaacttc accttcctac agccctacat
1021 caccctctta cagtccaaca tctccatcat atagtcctac atcacctgct tacagcccca
1081 catcgcctgg atacagccca acctcaccct catatagccc aacctcgcca tcgtatagcc
1141 ctacttcccc tagttacaat ccacagtcag caaagtatag tccttcgtta gcatattctc
1201 caagcagtcc gagattgtct ccatcaagtc catatagtcc cacatcaccg aactataggt
1261 gagtaattct ctggattttt taatatttat tttcggtact gaatgtgata tacagaacct
1321 tcaaacactg ggctatgcaa aacaatgaat ctcttctttc cctttatttt taagtcaaga
1381 acttgggttt ttggaggaac ctcaaccttt tgcaatagga gttattttac tttgtacatt
1441 ccatatttgc tcaagtcctg cgtttttcgt ttctcaattg catatttact cctagcatat
1501 gtctaatttg ttattttcca gcccaacgtc gccatcatat tccccaacat ctccatctta
1561 ttctccatca agcccaacat acagtcccag caggtaaatt aacttcattt actgtcctgc
1621 ctatcctttg aacagtttat ctcatgtctg tgacaagaat agtagtagaa ttgtagactt
1681 ttttattctc ttttcttttt atttgttaaa atttaagagt ttttaatatg tgacttattc
1741 atttttttcc cttttatcta gcccttataa ttctggtgtg agcccagact atagcccaag
1801 ttcgccccaa tttaggtata tgcatatata cacctcattc ttttaacata attgtataga
1861 tggaaggtat attgctgagc acggtctgtg ttatttgtct gtttgatcat ttgcagtccc
1921 agtacgggtt actcaccaag tcaacctggt tactcaccat cttcaaccag ccagtacact
1981 ccacaaacta gtgacaagga tgatggaggc actcgctgag gtaaactaac agctctgctg
2041 aagcgcaaat ttatgactaa aagtttttat cttattaggt ggagcattta tgatctaaat
2101 agttcagaaa ttcttcactg gagaaatgtt gactgaaaaa aattcctgat tctcctccat
2161 cttctcctat tggttcaaaa ttgtgaaatg agggtctatt tggccaaagt tctggtagtg
2221 gccggaaact atcagatttc tagaaatccc catattttaa tgaaaatttg ctgttaattt
2281 tggagggggg gaaatatgat ttagattttg gactagaaaa cttgggatgg ctgtaaacaa
2341 gtggtatgca tttggcattt tcctaatgta ttgagcatgt gaaaaagttc tcagtttgaa
2401 tttttaatag aaaagtacct aagtccggtg aaaatgttca ggacaacttg aggctggaca
2461 gaggacttat gctatgctgg gatcagacat tgagctgttc gagggctatt agtttgcatt
2521 gtattaatca tctgtatcat ttgagtctct gttgacaatg tcttcggaac agtttgtcga
2581 agtatgaatt caaattctgc tttgtacttg aacctttttt gaggcatgtg ttgtgattgg
2641 tcatcctctt aaatacattt attcaacatg tgcatgtaaa gttttgagga tgatattgat
2701 cactataaaa atatacattt gtaatatgtt acatcggttc gaatgttgaa cataaacgat
2761 atccggacct acttataata taaggggtat aatctggttc atcttaaatt tgttctttaa
2821 at
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