GenBank-Updates@genbank.bio.net (05/26/91)
LOCUS PHVDLEC1 1395 bp ds-DNA PLN 26-MAY-1991
DEFINITION Phaseolus vulgaris dlec1 gene for phytohemagglutinin (PHA-E)
(erythroagglutinating phytohemagglutinin)
ACCESSION X02408
KEYWORDS glycoprotein; hemagglutinin; lectin; phytohemagglutinin;
signal peptide.
SOURCE Phaseolus vulgaris DNA.
ORGANISM Phaseolus vulgaris
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Rosidae; Rosales; Fabaceaea.
REFERENCE 1 (bases 1 to 1395)
AUTHORS Hoffman,L.M. and Donaldson,D.D.
TITLE Characterization of two Phaseolus vulgaris phytohemagglutinin genes
closely linked on the chromosome
JOURNAL EMBO J. 4, 883-889 (1985)
STANDARD full automatic
COMMENT EPD; 14006; Pv[dlec1] PHA-E. SWISS-PROT; P05088; PHAE$PHAVU.
Data kindly reviewed (06-MAR-1986) by D.D. Donaldson
From EMBL entry PVDLEC1; dated 06-JUL-1989.
FEATURES Location/Qualifiers
repeat_unit 10..29
/note="imp. inverted repeat A"
misc_feature 12..30
/note="RY repeat (alternating pyrimidines and purines)"
repeat_region 12..26
/note="direct repeat 1"
repeat_unit 74..93
/note="imp. inverted repeat A'"
misc_feature 74..91
/note="RY repeat"
repeat_region 76..90
/note="direct repeat 1"
promoter 269..273
/note="pot. 'CCAAT' element"
promoter 332..337
/note="pot. TATA box"
misc_feature 360..364
/note="cap region"
misc_feature 367..377
/note="RY repeat"
CDS 375..437
/note="signal peptide (aa -21 to -1)"
/codon_start=375
CDS 438..1199
/note="erythroagglutinating phytohemagglutinin (aa 1-254)"
/codon_start=438
misc_feature 471..479
/note="glycosylation site"
misc_feature 615..623
/note="glycosylation site"
misc_feature 675..683
/note="glycosylation site"
repeat_unit 923..930
/note="inverted repeat B"
misc_feature 939..944
/note="pot. transposable element insertion site"
repeat_unit 949..956
/note="inverted repeat B'"
misc_feature 1294..1299
/note="polyadenylation signal"
misc_feature 1298..1304
/note="polyadenylation signal"
BASE COUNT 366 a 394 c 243 g 392 t
ORIGIN
1 actggccctg ctgcatgcat gcatgcacgc tgccaccaca gctttctcct cttttcacta
61 tgacacgact ggccatgcat gcatgcatgc ggccacctca gctccctcct cttcacccgt
121 gttttccaat gctacgtgcc aaccgcttct ctctataaat atctctttaa atttaaacta
181 attatttcat attttttcaa tgtatttgat gacgtgtatg cattgccatc gttgcttaat
241 tgttatttct atattcttat tactccctca aataatatta taaaagaaaa aagaatccat
301 aaagtagaga gagagagtgc agttgttgtt gtataaatag agaagagagt gatggttaat
361 gcatgaatgc atacatggct tcctccaact tactctccct agccctcttc cttgtgcttc
421 tcacccacgc aaactcagcc agccaaacct ccttcagctt ccaaaggttc aacgaaacca
481 accttatcct ccaacgcgat gccaccgtct catccaaagg ccagttacga ctaaccaatg
541 ttaatgacaa cggagaaccc acgttgagct ctctgggccg tgccttctac tccgccccca
601 tccaaatctg ggacaacacc accggcgccg tggccgcttc gccaacctcc ttcacattca
661 atatcgacgt tcccaacaat tcaggacccg ccgatggcct tgcctttgtt ctcctccccg
721 tgggctctca gcccaaagac aaaggcggtc ttctaggtct gttcaacaac tacaaatacg
781 acagcaatgc ccatactgtg gctgtggagt tcgacaccct ctacaacgtt cactgggacc
841 ccaaaccgcg tcatattggc atcgacgtga actccatcaa gtctatcaaa acgacgacgt
901 gggattttgt caaaggagaa aacgcggagg ttctgatcac ctatgactcc tccacgaagc
961 tcttggtggc ttctctggtt tacccttctc tgaaaacaag cttcatcgtc tctgacacag
1021 tggacctgaa gagcgttctt cccgagtggg tgatcgttgg gttcactgcc accactggga
1081 ttactaaagg gaacgttgaa acgaacgaca tcctctcttg gtcttttgct tccaagctct
1141 ccgatggcac cacatctgaa gctttgaatc ttgccaactt cgccctcaac caaatcctct
1201 agactcaatc tccaccttcc ctgtgacact aaatcactgc ttacactcag tgttctttcc
1261 tgctaataat gtttctcttt gtctgtcaca gaaaataaaa taaaataggg tgatgatagc
1321 ttacactcag tgttctttgc tctacttcct taattcctta atgaaaatat aataaaaaat
1381 aataattaaa atatt
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