GenBank-Updates@genbank.bio.net (05/26/91)
LOCUS PHVDLEC2 1661 bp ds-DNA PLN 26-MAY-1991
DEFINITION Phaseolus vulgaris dlec2 gene for phytohemagglutinin (PHA-L)
(leucoagglutinating phytohemagglutinin)
ACCESSION X02409
KEYWORDS glycoprotein; hemagglutinin; lectin; phytohemagglutinin;
signal peptide.
SOURCE Phaseolus vulgaris DNA.
ORGANISM Phaseolus vulgaris
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Rosidae; Rosales; Fabaceaea.
REFERENCE 1 (bases 1 to 1661)
AUTHORS Hoffman,L.M. and Donaldson,D.D.
TITLE Characterization of two Phaseolus vulgaris phytohemagglutinin genes
closely linked on the chromosome
JOURNAL EMBO J. 4, 883-889 (1985)
STANDARD full automatic
COMMENT EPD; 14007; Pv[dlec2] PHA-L. SWISS-PROT; P05087; PHAL$PHAVU.
Data kindly reviewed (06-MAR-1986) by D.D. Donaldson
From EMBL entry PVDLEC2; dated 06-JUL-1989.
FEATURES Location/Qualifiers
misc_feature 296..304
/note="RY repeat (alternating pyrimidines and purines)"
repeat_region 305..317
/note="imp. direct repeat 1"
misc_feature 340..348
/note="RY repeat"
repeat_region 349..361
/note="imp. direct repeat 1"
misc_feature 392..408
/note="RY repeat"
repeat_region 393..408
/note="imp. direct repeat 2"
repeat_region 409..421
/note="imp. direct repeat 1"
repeat_unit 469..485
/note="imp. inverted repeat A"
promoter 483..488
/note="pot. TATA-box"
repeat_unit 488..504
/note="imp. inverted repeat A'"
repeat_region 512..527
/note="imp. direct repeat 2"
misc_feature 513..517
/note="capping region"
misc_feature 520..530
/note="RY repeat"
CDS 528..587
/note="signal peptide (aa -20 to -1)"
/codon_start=528
CDS 588..1343
/note="leucoagglutinating phytohemagglutinin (aa 1-252)"
/codon_start=588
misc_feature 621..629
/note="glycosylation site"
misc_feature 765..773
/note="glycosylation site"
repeat_unit 1067..1074
/note="imp. inverted repeat B"
misc_feature 1083..1088
/note="pot. transposable element insertion site"
repeat_unit 1093..1100
/note="imp. inverted repeat B'"
misc_feature 1433..1438
/note="pot. polyadenylation signal"
misc_feature 1438..1443
/note="pot. polyadenylation signal"
misc_feature 1442..1447
/note="pot. polyadenylation signal"
BASE COUNT 472 a 406 c 306 g 477 t
ORIGIN
1 ttttgttaat aaaatgaaag ttaatattta tggatttatt tgaacatatt tacaagtttt
61 tgttaataaa tttatttaca gaagtgttaa atttgaaaat gtgttaaatg agagttaata
121 tttatggata taatattact ttatttaaaa ataaaatgaa tttatattaa aatatgattt
181 ggtaaaagat attataaaga ggaacttgga tgagaaagac gacgatgggc aaaagttgca
241 tggcacaccg cttttgttag taacatctgc actgtggcgc ctttttcaca ctcaccatgc
301 atgctgccac gtcagctttc acactatgac accactggcc atacacgttg ccacctcagc
361 tttctcctct tctcactatg acacgactgg ccatgcatgc atgcatgctg ccacctcagc
421 tcccgcctct tcacccgtgt ctttctctca tgtcgcagtg ctgcgtgcca acctcttctc
481 tctataaata gagagaagag aatgatggtt aatgcatgaa tgcatacatg gcttcctcca
541 agttcttcac tgtcctcttc cttgtgcttc tcacccacgc aaactcaagc aacgatatct
601 acttcaactt ccaaaggttc aacgaaacca accttatcct ccaacgcgat gcctccgtct
661 catcctccgg ccagttacga ctaaccaatc ttaatggcaa cggagaaccc agggtgggct
721 ctctgggccg cgccttctac tccgccccca tccaaatctg ggacaacacc accggcaccg
781 tggccagctt cgccacctcc ttcacattca atatacaggt tcccaacaat gcaggacccg
841 ccgatggact tgcctttgct ctcgtccccg tgggctctca gcccaaagac aaagggggtt
901 ttctaggtct tttcgacggc agcaacagca atttccatac tgtggctgtg gagttcgaca
961 ccctctacaa caaggactgg gaccccacag agcgtcatat tggcatcgac gtgaactcca
1021 tcaggtctat caaaacgacg cggtgggatt ttgtgaacgg agaaaacgcc gaggttctga
1081 tcacctatga ctcctccacg aatctcttgg tggcttctct ggtttaccct tctcagaaaa
1141 cgagcttcat cgtctctgac acagtggacc tgaagagcgt tcttcccgag tgggtgagcg
1201 ttgggttctc tgccacaact gggattaata aagggaacgt tgaaacgaac gacgtcctct
1261 cttggtcttt tgcttccaag ctctccgatg gcaccacatc tgaaggtttg aatctcgcca
1321 acttggtcct caacaaaatc ctctagactc caaactccac cttccctgtg acactaaaac
1381 actgctttca ctcagtgttc tttcctgcta ataatgtttc tctgtcacag aaaataaaat
1441 aaataaaatg ggagctcata tattattata ctattaaaag gaagctgtca aatgtttgtg
1501 ttttactcca aactttgact gttaaagaaa agttttccaa aatagagttt acacgcgtta
1561 ttagtacaca atgtaatttt ttaaaaagtt ttatagtaat gaaaaggatt attccaagtt
1621 aaagtataac acagattaaa gtattaacca ttagaaaaaa a
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