GenBank-Updates@genbank.bio.net (05/26/91)
LOCUS PEAGPA2 3696 bp ds-DNA PLN 26-MAY-1991
DEFINITION Pea gpa1 gene for subunit A of chloroplast
glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13)
ACCESSION X52148
KEYWORDS dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase;
photosynthesis.
SOURCE Pisum sativum DNA.
ORGANISM Pisum sativum
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Rosidae; Rosales; Fabaceaea.
REFERENCE 1 (bases 1 to 3696)
AUTHORS Cerff,R.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Liaud,M.-F., Zhang,D.X. and Cerff,R.
TITLE Differential intron loss and endosymbiotic transfer of chloroplast
glyceraldehyde-3-phosphate dehydrogenase genes to the nucleus
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87, 8918-8922 (1990)
STANDARD full staff_review
COMMENT *source: strain=rosakrone; developmental stage=2 week seedlings;
*source: tissue=green photosynthetic; library=EMBL4; *source:
clone=EMBL4-Gpa1; See <X15190> for GAPA mRNA sequence.
From EMBL entry PSGPA1; dated 06-DEC-1990.
FEATURES Location/Qualifiers
precursor_RNA <3663..3662
/note="primary transcript"
mRNA <1583..1582
/note="exon 1"
promoter 1442..1448
/note="put. TATA box"
CDS 1541..1582
/note="GAPA precursor polypeptide"
/codon_start=1541
CDS 1541..1582
/note="transit peptide (AA -68 to -55)"
/codon_start=1541
intron 1583..1676
/note="intron I"
mRNA 1677..1793
/note="exon 2"
CDS 1677..1793
/note="GAPA precursor polypeptide"
/codon_start=1677
CDS 1677..1793
/note="transit peptide (AA -54 to -16)"
/codon_start=1677
intron 1794..2008
/note="intron II"
mRNA 2009..2319
/note="exon 3"
CDS 2009..2053
/note="transit peptide (AA -15 to -1)"
/codon_start=2009
CDS 2054..2319
/note="GAPA mature protein (AA 1 to 89) (2319 is 2nd base
in codon)"
/codon_start=2054
intron 2320..2630
/note="intron III"
mRNA 2631..2875
/note="exon 4"
CDS 2631..2875
/note="GAPA mature protein (AA 90 to 171) (2631 is 3rd
base in codon) (2875 is 1st base in codon)"
/codon_start=2631
intron 2876..2959
/note="intron IV"
mRNA 2960..3662
/note="exon 5"
CDS 2960..3459
/note="GAPA mature protein (AA 172 to 337) (2960 is 2nd
base in codon)"
/codon_start=2960
misc_feature 3641..3646
/note="polyA signal"
polyA_site 3662..3662
/note="polyA site"
BASE COUNT 1177 a 632 c 645 g 1242 t
ORIGIN
1 gtcgactcag attcaaaccc tgtcttgaat ttttgtttca ttgttttttc cgatttgttt
61 ttgaaaattc attatccaat tccatggggc tcatttcctg gtttgaaatt cgaacttaaa
121 attttgaaag gaacacattc gcatgttcta aacattcctc gtcaaccaat cttatttttt
181 ttaagttaag tacttttaaa atagagaatg tcatagtatt tattagcaat ataatgatat
241 aatcaaatct cacacataaa tatttgtgaa aaagtataat aataaaattt ggttgatatt
301 tgtgaaatat cgtaagaatt attataacga aatagtatag taataaagtt ttattgattg
361 tatttgtaat atttatgaaa atatcgtaaa aaattaaagt aaaaaatgtt tgtggttctt
421 ttaaatatct caaattttat ttttttatcc tcaaaatatt tcttttaaat aatgttcttt
481 ctaaaatttt ccatccacat ttttagtccc taatactaaa actgtcgtta attcagtcgc
541 taagtaataa atcatcgtta aaatcgtcgt taattcagtc attaaattat aagaatcgtc
601 gctaagttgt aggagagatt aaaagtgtgg atgaaaattt ttaagatgat cattctttga
661 aagaaatatt tcaagggact aaaaacgaaa tttgagatat ttaatcctaa gaattattat
721 aaataatagt aaaattgtat tgattgtatt tgttaaactt ttttgcatat catttaattc
781 ataatataaa attccttgca taaaactatg aagggagagt aagattgaga tacttactat
841 ggagtaagca tatccaaacc tatcatacac atgtgtgtta ggatgtatgg gtgtgattat
901 taacgagctt aagaaaaaaa acaaaaagcg caaattggat tggtggcttg tgagcaaact
961 tgaaacccaa cacttgaaac ccaacttgga accaaatgaa gagtagccat aacattgttc
1021 tctttgtcca gtagtatctc tcctgtcaca tacatttctt tgtagtttcc ttttctttta
1081 agacttcaat gactaggcac ttgttagata aaataatttt acagttatct aatataaaaa
1141 aaatccatca tcttaccata tagaaaaata ttatttaaaa aaaaaatata gaattttaga
1201 atgtcacata tatgaagatt ttggcccaac catcatttta caatagaaaa aaaaaatgag
1261 tgagtgagag tgagatatca tgatgattga agttactaga aacacaaaag caaagcatga
1321 attacagcca ttcatgaatg atattgctat ccaaatctaa tccttacttg tttgggctca
1381 tgagtcacaa ccacacatcc acgttgccac atcttatttc cttactatac ttcatactac
1441 ataataatac taacatttaa ctaagacttt gcttctctgc aaaacttgta tactacaaac
1501 ttcaccttca aaatattcag tgttccttgt tggctcaatc atggcttcgg ctactttctc
1561 tgtagccaaa ccagctatta aggtccagta taactctagc tttactataa ataaggttca
1621 tgcagaagcc atatttatat ttacactagt ttttcttttt ctttgctttg ttttaggcaa
1681 atgggaaagg cttctctgaa ttctctggtc tccgcaactc ttcaagacat cttccctttt
1741 ctagaaaatc ttcagatgat tttcattctc ttgttacctt ccaaaccaat gcagtaagtt
1801 ccttatctcc attgaatgac taatactttt gttattgtaa aaatgaagaa cttgatcata
1861 gtatgtgtga gaataaaccc tagggtcccg agagagtgat cctgagagtg agagtgtgat
1921 tctctcaaga tctcaaattt gaattctgct aagtgacatg ttttacaatt ttgcatctat
1981 ctatcaatct atatatgtat atttgaaggt tggaagtagt ggaggacaca agaaaagtct
2041 tgtagtggaa gcaaaacaac tgaaggtagc cataaatgga tttggaagaa ttggaaggaa
2101 cttcttgaga tgttggcatg gtcgcaagga ctcgcctctt gatgtcattg caatcaatga
2161 caccggaggt gtaaagcaag cttctcacct tctcaagtat gattccacac ttggaatctt
2221 tgatgctgat gttaagcctg ttggtactga tggcatctca gttgatggaa aggttatcaa
2281 agttgtctcc gaccgcaacc ctgccaacct tccttggaag taagctattc tctcattatt
2341 gatttatgaa catgttataa gttatttcaa taagctttgt ctcataaatg atcatgttac
2401 tagataactt taaatatatc aatctaaata gacccttaag tagataagaa cttctccaat
2461 gctcatggag aaaattgtct aaagaatata cattagtgat tgatcaataa ggacatttaa
2521 atcatgtatg tatgaatcat acatcatcat tgtgtatcga ttgttggtta ccgatttatt
2581 aagcagcgac acacaggcta taaagattgc tatatattga tattttgcag ggagttgggg
2641 atagacttgg tgattgaagg aactggagtg tttgtggaca gagaaggtgc agggaggcac
2701 attacagcag gggctaagaa ggttctcatc actgcccctg gaaaaggaga catccctact
2761 tatgtggttg gtgtcaatgc tgatgcttac acccacgccg acgacattat cagcaatgct
2821 tcttgcacca ctaactgcct tgctcccttt gtcaaggtcc ttgatcagaa attcggtaat
2881 gtaccttttc accacatttt ttaacaagga tatgtacttt tttattgttt ataacatttg
2941 aaaatgtatt gggcaatagg tatcatcaag ggtaccatga ctactactca ctcctacacc
3001 ggtgaccaac ggcttcttga cgcgagccac cgtgacctaa ggcgtgcgag agcagcagcc
3061 ctcaacatag tcccaacatc aacaggagca gctaaagcag tggcccttgt cctcccaaca
3121 ctcaaaggca agctcaacgg tattgcgctt cgtgtgccaa caccaaacgt ttcggtggtg
3181 gacctcgtcg ttcaagtctc aaagaagaca tttgctgaag aagtgaatga ggcttttaga
3241 gagagtgcag ccaaggagct gactggtatt ctctcggtct gtgacgagcc actcgtgtct
3301 gtagatttta ggtgcaccga tgtgtcgtct accgttgatt cgtcattgac aatggtcatg
3361 ggcgatgacc tggttaaggt gattgcttgg tatgataatg agtggggtta ctcacaaagg
3421 gttgttgatt tggctgacat tgttgccaat aactggaagt aagagccaaa taaggtttgt
3481 taattatatc tatatctata tatatatata tatatatata tatatatata tatgtacgta
3541 ccatgtgcca ttgtaatttt gtacacttct tgcttatttt tttctttcta aacttgtatt
3601 gagaacagaa ccatcaattt tctcttgctc ctcattgtgc aataaattgt tttaccttgc
3661 tgaaatatac atgttgatac taagcttcca aatcta
//