GenBank-Updates@genbank.bio.net (05/26/91)
LOCUS PHVAPHASE 4764 bp ds-DNA PLN 26-MAY-1991
DEFINITION Phaseolus vulgaris gene for alpha-phaseolin
ACCESSION X52626
KEYWORDS alpha-phaseolin; glycoprotein; phaseolin; seed storage protein.
SOURCE Phaseolus vulgaris DNA.
ORGANISM Phaseolus vulgaris
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Rosidae; Rosales; Fabaceaea.
REFERENCE 1 (bases 1 to 4764)
AUTHORS Anthony,J.L.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1987 to 4764)
AUTHORS Anthony,J.L., Vonder,H.R.A. and Hall,T.C.
TITLE Nucleotide sequence of an alpha-phaseolin gene from Phaseolus
vulgaris
JOURNAL Nucleic Acids Res. 18, 3396-3396 (1990)
STANDARD full automatic
COMMENT *source: strain=Sanilac; tissue=leaf; clone=9C;
From EMBL entry PVAPHASE; dated 18-JUN-1990.
FEATURES Location/Qualifiers
promoter 2196..2207
/note="put. CAAt box"
promoter 2232..2246
/note="put. TATA box"
precursor_RNA 2275..>2274
/note="primary transcript"
mRNA 2275..2664
/note="exon 1"
CDS 2352..2664
/note="precursor polypeptide (AA -22 to 82) (2664 is 1st
base in codon)"
/codon_start=2352
CDS 2352..2417
/note="signal peptide (AA -22 to -1)"
/codon_start=2352
CDS 2418..2664
/note="alpha-phaseolin (AA 1-82)"
/codon_start=2418
intron 2665..2736
/note="intron I"
mRNA 2737..2927
/note="exon 2"
CDS 2737..2927
/note="alpha-phaseolin (AA 83-146) (2737 is 2nd base in
codon)"
/codon_start=2737
intron 2928..3015
/note="intron II"
mRNA 3016..3096
/note="exon 3"
CDS 3016..3096
/note="alpha-phaseolin (AA 147-173)"
/codon_start=3016
intron 3097..3220
/note="intron III"
mRNA 3221..3451
/note="exon 4"
CDS 3221..3451
/note="alpha-phaseolin (AA 174-250)"
/codon_start=3221
intron 3452..3579
/note="intron IV"
mRNA 3580..3838
/note="exon 5"
CDS 3580..3838
/note="alpha-phaseolin (AA 251-336) (3838 is 1st base in
codon)"
/codon_start=3580
intron 3839..3941
/note="intron V"
mRNA 3942..>3941
/note="exon 6"
CDS 3942..4156
/note="alpha-phaseolin (AA 337-408) (3942 is 2nd base in
codon)"
/codon_start=3942
misc_feature 4084..4137
/note="repeat region"
BASE COUNT 1648 a 897 c 782 g 1437 t
ORIGIN
1 ttaacccact ttctccccac tttctcccca cctcatgccc ctccccaacc aagatgaaca
61 ctcacttgac atttccatga aaagattccc ttggtctgtg caggaagtgt aatgtcgatc
121 accacatatt cctactgaaa tcaccacata ccgccactct ataacttgag tgaattttat
181 ggttagttca cactagccta ctatcatggc cctataaaga cctcatttaa cccactttct
241 ccccacctca tgccctccaa aaccaacacg gacactcact tgacatttca gaaaaaattt
301 ccctgggtat gtgcaggaag tctaatgtgg gtcaccatat attcctacta tggtcaccat
361 atattgtcac cctttaagtt caatgaattt tttgcttact caacaatgcc ctacactctt
421 ggcctataaa aaccttatgt aactcacttt ctctctacat catgccccac ccaaccaaaa
481 tagcttcaga aaatgtattt tttttgaaga aaaaaaaaac taaaaaagca tacatagtta
541 cacaaactaa gtaaatgcaa atatatattt ttaacaatat attcaaaaaa aaaaacaagt
601 catgctccat aaaaaactaa atagtaaact cagataacta taaccaaatc acatttaaac
661 acagacttta actcatacaa gggtcaacac atgacaaata acacagatca cccaatacaa
721 agcacaatat gaatgtttca cttaataaag aaatgaatag aaaatgtatt acctttggtc
781 accaatccct aaccccacgg tgagagagaa ggtggaggaa aaaccttgag aagaagatgg
841 aagaggacga cacagacaag aacatgaaga aaaatgaaga agaataaaga agaatgcgca
901 cacacttgca acatcccatg gagaattaaa gggaacaaaa acggaacgaa caaaaaatga
961 atggtaagaa cacaggaaaa aggaaatagg gacgtgaaga agaaaagtga agaagtgccc
1021 caatttctta gctctaattt cttcacattc tgaccctaaa ataaaatgtt aaatctattc
1081 aacacgtaac ccacaactgc aataaaataa ttttgattgt cacataggcg attatatggt
1141 gaacaagtgc aggaccgatt gttcaaccat cattttttgt ataaataatg atgaagatct
1201 cattacaata gcattatatt catattaaag aataggaaaa aattcatata tacttagcac
1261 tatattataa taatataata atatttttaa ttaaaaaata aattaaatat gattaaaata
1321 ttattttatt gatttgtgaa atagattttt tttattgatt gtggtgtgaa aaccatcacc
1381 gtcctaaaga agaaagaata taagagtggt aagctgagtc gactagtctt ttaacgaggt
1441 gttggacact ttggtaagta cgtcttttgg atatacatgg attctgtgat cttgaacagc
1501 tggttgataa agaaaaggaa gtgaaggtga taacggagga gagatccgct atacacaagt
1561 gataagaaca aattactgac atcaaaactt ggaaaattgc aaacgaatgc cattcaaaac
1621 ccacatggga cataattctt acatacaatt acattcattt aacatcttaa aaaaaaatca
1681 tttaacatct taaaaaaatt attatattgt tttattataa taggatattt tgttctatta
1741 aaattttatg gaatatatgt aattcttaag aataatgtta agagtaaatt tttcaaaatt
1801 tatttgacaa aaaaaatggt aaaacatata ctattaagtt gatttttcgt aaaatagaga
1861 caaatgcaac cgcacccttc ttcaatcaca caaatttggt tcaggttgtc atggcactct
1921 gtagtcgttt ggttcatgca tgggtcttac gcaagaaaaa gacaaagaaa aaagccaaaa
1981 cagagagatc gccgcgtcca tgtatgtcta aatgccatgc acagcaacac gtgcttaaca
2041 tgcactttaa atggctcacc atctcaaccc acacacaaac acaatgcctt tttcttcatc
2101 atcaccacaa ccacctgtat atattcattc tcttccgcca cctcaatttc ttcacttcaa
2161 cacacgtgaa cctgcatatg cgtgtcatcc catgcccaaa tctccatgca tgttccaacc
2221 accttctctc ttatataata cctataaata cccctaatat cactcacttc tttcatcatc
2281 catccatcca gagtactact actctactac tataataccc caacccaact catattcaat
2341 actactctac tatgatgaga gcaagggttc cactcctgtt gctgggaatt cttttcctgg
2401 catcactttc tgcctcattt gccacttcac tccgggagga ggaagagagc caagataacc
2461 ccttctactt caactctgac aactcctgga acactctatt caaaaaccaa tatggtcaca
2521 ttcgtgtcct ccagaggttc gaccaacaat ccaaacgact tcagaatctt gaagactacc
2581 gtcttgtgga gttcaggtcc aaacccgaaa ccctccttct tcctcagcag gctgatgctg
2641 agttactcct agttgtccgt agtggtaagt aattgctact ggtatcactt gtttcttctt
2701 gcagaaataa tggtaatgag ttttttataa tttcagggag cgccatactc gtcttggtga
2761 aacctgatga tcgcagagag tacttcttcc ttactagcga taacccgata ttctctgatc
2821 accagaaaat ccctgcagga accattttct atttggttaa ccctgatccc aaagaggatc
2881 tcagaataat ccaactcgcc atgcccgtta acaaccctca gattcatgta ctgccttttg
2941 taatactgaa ctaatttttt gttattttaa cttgcaattt ctctccaaat gtgatgataa
3001 atgtttgtcc tgcaggactt tttcctatct agcacagaag cccaacaatc ctacttgcaa
3061 gagttcagca agcatattct agaggcctcc ttcaatgtaa gaaagaaaac agcatctaac
3121 tacatatttg cgttgccatt tagctagtac tttgtctaaa tgtcacactt gttgaatttg
3181 ttgaatgata tcattatata tgtttgcatg atttttatag agcaaattcg aggagatcaa
3241 cagggttctg tttgcagagg agggacagca agagggagtg attgtgaaca ttgattctga
3301 acagattgag gaactgagca aacatgcaaa atctagttca aggaaatccc tttccaaaca
3361 agataacaca attggaaacg aatttggaaa cctgactgag aggaccgata actccttgaa
3421 tgtgttaatc agttctatgg agatgaaaga ggtaaataca aagaaaaacc atatagacaa
3481 actcagcaat tgagttctat tattcactgt cgtcttggtt agaaaatctt agtattgaga
3541 ctataattaa ataatggttt tttttgttaa caaatttagg gagctctttt tgtgccacac
3601 tactattcta aggccattgt tatactagtg gttaatgaag gagaagcaca tgttgaactt
3661 gttggcccaa aaggaaataa ggaaaccttg gaatatgaga gctacagagc tgagctttct
3721 aaagacgatg tatttgtaat cccagcagca tatccagttg ccatcaaggc tacctccaac
3781 gtgaatttca ctggtttcgg tatcaatgct aataacaaca ataggaacct ccttgcaggt
3841 atatatattt attatatatg accatgaatt tgaatatagg gttgttgatg ggatttttta
3901 tttataattg gtaatgcgtg attgtgattg aaaatatgaa ggtaagacgg acaatgtcat
3961 aagcagcatc ggtagagctc tggacggtaa agacgtgttg gggcttacgt tctctgggtc
4021 tggtgaagaa gttatgaagc tgatcaacaa gcagagtgga tcgtactttg tggatggaca
4081 ccatcaccaa caggaacagc aaaagggaag tcaccaacag gaacagcaaa agggaagaaa
4141 gggtgcattt gtgtactgaa taagtatgaa ctaaaatgca tgtatggtgt aagagctcat
4201 ggagagcatg gaaatatgta tccgaccatg taacactata ataactgagc tccatctcac
4261 ttcttctatg aataaacaaa ggatgttatg atatattaac actatatgca ccttcactag
4321 taatacatta atatttaata ctttttattt taacttttta gtttaaaata ttattatatt
4381 attaactttt tagtttaaaa tatttatatt attataaaga gaaataaaca aaggatgtta
4441 tgatatatta acactatatg taccttacat agtaatatat taatatttaa tactttttat
4501 tttaactttt taatttaaaa tattattata aatgacgctt gtgttttatg tgttggcatg
4561 cttgtatttt atgtgttgac tttctgtgtg aaggtaatgt gatatggtga gctggtggta
4621 acaattgtgt tttatgtgtt ggctttctgt gaagctaatt tgatatggtt agctgatgtg
4681 aacaaaatat taaaggaagc taatttgata tggttagccg atagtaacaa aatatcaaaa
4741 taaatttctt cttactttaa taaa
//