GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS BLYBDG 6260 bp ds-DNA PLN 18-MAY-1991
DEFINITION Barley DNA for (1-3,1-4)-beta-D-glucanase (EC 3.2.1.73)
ACCESSION X52572
KEYWORDS (1-3,1-4)-beta-D-glucanase; glucanase.
SOURCE Hordeum vulgare DNA.
ORGANISM Hordeum vulgare
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
Commelinidae; Cyperales; Poaceae.
REFERENCE 1 (bases 1 to 6260)
AUTHORS Litts,J.C.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 6260)
AUTHORS Litts,J.C., Simmons,C.R., Karrer,E.E., Huang,N. and Rodriguez,R.L.
TITLE The isolation and characterization of a barley 1,3-1,4-beta-
glucanase gene
JOURNAL Eur. J. Biochem. 194, 831-838 (1990)
STANDARD full automatic
COMMENT *source: strain=Klages; clone=lambda HV29;
From EMBL entry HVBDG; dated 21-FEB-1991.
FEATURES Location/Qualifiers
promoter 1854..1860
/note="put. TATA box"
precursor_RNA 1886..>1885
/note="primary transcript"
mRNA 1886..2049
/note="exon 1"
CDS 1977..2049
/note="(1-3,1-4)-beta-D-glucanase (AA 1-24) (2049 is 1st
base in codon)"
/codon_start=1977
intron 2050..4539
/note="intron I"
mRNA 4540..>4539
/note="exon 2"
CDS 4540..5468
/note="(1-3,1-4)-beta-D-glucanase (AA 25-334) (4540 is 2nd
base in codon)"
/codon_start=4540
BASE COUNT 1667 a 1550 c 1414 g 1629 t
ORIGIN
1 ggatccggta ttctcaacca tatcggataa gtacaatttt tggtttgtat cagataaata
61 ggtttggact ttcaggtttt ggtaagcatg ggagtctgta atcctggtgg taagctgctg
121 atcctccctt tttctgaaag aaaaaaaaaa ggttactaac cctttgaatg aatcaggaca
181 aactaagcct ctcaagtgga aattcggaac caatgatgtg ccagaaacta tcttggctac
241 atgcacggta cgaattggca gcatcatcat aactacaact tgatcaatat acaactcact
301 acaaaacaca tgtctcaaga agaccatttt agcatatagg accaattcag tacatgtgcc
361 aaaagagggc agcccggctt gcatctgtca aaaagttggg ctgctaagcc accaatagga
421 aatttctcat caaccagccg tatatgcatg tgtgttaacc gaaaccaata tgcccaagca
481 gaccacctgc atgctagcta atcctagaca cgaccggctc ttaaatacaa ttggtccggt
541 cgaataattc gttcaccgaa atgagtcccg agtcatcgta cagtttttat attcgtatta
601 actgtaatct acacatttgg tgcacctaat attatttcgt cattagcact tatactacaa
661 gctagagagc agttgcatga ccggccggac tctgctgata gcaacaacat actgtgtgta
721 aaagaactat caacggccgg atctggatat cccacctgat caataggatt gggagggaga
781 gacgtacgta cttagtttgt tgtcggggtt attacaacac gtatggtgcc acgagggaaa
841 accatgcatg cgttgcacca aaagctcaca tgtatggtgc gcttagacaa gccgtggtgt
901 tgtgttccta tggcaattcc taataaaggt cttctttgct cttttcaatt gcaaggccga
961 tgaactcaat accttctctt gtgagacaac aagtccaaaa gttaagtggg tctggtcttg
1021 tttgacaaag tataaagaca tgcttaacta aagtggtttg agtaattcta tgattctttt
1081 gacaaaaatc ttaattaatt aggtacacca aaaacacaaa tgttcttatg ataaacaaaa
1141 ttgttggttt cttgcaatat aataacaaaa caatggcatg taaaaatacg agcatgtact
1201 acatatatac cggtatgaga cggtaaaagg tagagagacc aaaggcttct gatgtactcc
1261 aaatctcttt aaattgaccc cgaatatcca ttactaaaat ataatttcat ttgtatacat
1321 gtcacttcac gataagaaac aacaaagccg acaaaaccgt tgcgcccggt tctttcctac
1381 gtaagtcatg tcaacttcaa agataaaaaa aaaatcattg ccaacaagtc tccgttgtgt
1441 caattcttct tacgtaagtt atgtcggatt ccaccgaaca cggtccttgc gtgaaaaaac
1501 cgccgccgaa tgtcgttgag taagacgtga acatacccga cgccgcgcga cccatcattg
1561 acctagaaac ttcactttca tggtacatca tgggtggagt ccaaaattca aactattttt
1621 tcaaaagttg tttggtacca ctatgaatga atgaattatc cccttcccct acctgcaaca
1681 acaacctggt gtaccggata acctctgccc accaccagac acacactgtg agaaggcggt
1741 gacgcatgca aaccagccta ggtagtcaat cgcaacaggc taaataaatg tcgctggagg
1801 cgttgggcct gcgctcccga gtggattgga ccgaactatg tctcctcgga tcctatataa
1861 ggggcctgca ccccgttgtg gcctcaccag aaaagaaaca acaagagctt tacagagagc
1921 cttggcatca cccacccaca ccctcaccct ccaacgcagc tagagagaga aagagaatgg
1981 caggccaagg cgttgcctcc atgttggctc tggcattgct cctcggagcc ttcgcctcca
2041 tcccacaaag tgattcccct tccttccctc cctctctctc tcttgaagtg attgggtgca
2101 gttttttttt tcatgcaggg agtttctttg aagatagtaa tacgtacgtt gatcttagct
2161 ttcattaaga gaagcattag ggagctagct aggtagccgg cggccatggt gtacccatgc
2221 tcacataaac ctctctccca cagacgtcac atagcatacc ttcctctgtt taggtgcatg
2281 tcgctttcgt tctgcgtttt attcttcccg cgccatcagt cgcatggtaa agcatgaacg
2341 gccggcgccc gccggtctca ttatctacct cgatgcgtcc atcaatgcat gggagcacag
2401 taacttttca aaaaaaaaat cgaaaactaa acacggtaaa ttttgtaaaa aaaatcgttt
2461 tcactcgcta aaagaatcag gagtcttggg ttttgctgcg gagtatacgt atcaaaatat
2521 atgcaccgca cgctccaaga taaaagtgca cgtcagcacg tgtgatcggc cttcattgtg
2581 tttcttctat ggaagaaaac gttgcaagaa gtaggtattc cgcaggaaaa ttaatatctt
2641 aagaacaaca aaaatgatac tttctagata aaacattgaa agaggaagta agcagtgaaa
2701 acgatgcttg tagatagttt caactgtttt aaccataaat taatctagtt gatcgtacgc
2761 aaaagcttgc aaatgaagtc tcgaaatgtt tggtgaccat gcatgtcagt tagctagctg
2821 catcttttta caaagtcaga aggtttaact aacgacctac aagtagctag ctaggccagt
2881 gtctctttct ccagtgtcat gcatgctaat cacctagaaa gtttttcttc ttcttcctcc
2941 tcttccctgc atgcaaagct gtgcatgcat gtctcgacag tgtcgcccta gcactgtaga
3001 cacgagccct accacttaat tggtggtttg ttccctgatt aattcggtgc aactactgct
3061 gaaccacccg tcgctaacca tctttttctt cttcctttgg tccgcatctt gacgtttttt
3121 cagcgatgga cgatgccgta ccggccagtc cagtggtcct actcgtttca gctagccact
3181 gttccattct tttaagctta acgaagacga ttacaccagc tgctagctag ccagtaacta
3241 taactcaatt gcagcctttg tgtagtcatc atatcgactt tgaaagtgca accggtggat
3301 ccatggtaca tgacgccatc gatcactagg cactaactaa ccatgcataa cccaggtgtt
3361 ggttctgctg gcctttccaa gtttggtctg cctgaaaaga attgtgatat aataggattg
3421 taggttagtg tagtggtact agccagtagt tggcacttga tcggccgggc agcaagttaa
3481 gaagaggatt aagttgcgtg tacttatatg gagtactttg tcatgcacgt gagctagacc
3541 gtttattgga gcttagctgg gagcagccgg agggcatgca tgcacgccat ggcgatccac
3601 atcgatcgta tgtggactat ccaacggccg tgctgcggac gttggaccag aaactttggt
3661 ttgtcctagc atgtatgtat gcatgaggtc tctacacccc cctgtatttc taggcttcct
3721 gccaattgcc atctacgtgt ggtcgacctc cattctgccc ctacgatatt cctggcccgt
3781 tttgtttaat aaatccagct agctagtgcc gtgcaagtac tacacaattt atgccatacc
3841 attgattggc gatggcactc ttaagtgtac acgtactgta aaaatacatg tattctttct
3901 agcataattg atgtaccaac tatcaagtgt aacactttaa tagagtagat tggtgtagag
3961 ttagttggta aaccaagcaa atcagggagg gtaaaagatt cactgcactg gaagctagtc
4021 aaagttcatt ccttccttta aggttatcta gttccttttt ctccttgctc gtggtagagt
4081 agctagtagc tagtgacaag tcggtcaagg cgccggccgt gaaaatagca atgttcctcg
4141 gccgtgtgcg tgcatctgac accaactcgt gactgtaaca aaaacaatat ataagtgctg
4201 catcagccac caaagtctag agagaaagag ataaaaaaaa tgcgcaaagc tagaggctta
4261 cacgcatgca tccatgcatg cgtacaagat ttcagttaaa cgtccttttc ggggagttag
4321 tattatccct cgccaacagg tcaaaaggct ctgtgtgcat gtgtgttcat gcatcgccgc
4381 cattcttgct tattggtttc ttttttatat tccatcacat gccatcatgg gagaattttt
4441 taattttcta ctatggcaat ggaacagtgc tactactcta cctggtgtaa ataattgatt
4501 ttgtgaaggt taactaaccg aggttatatt acattgcagg cgtggagtcc atcggggtgt
4561 gctacggcat gagcgccaac aatctgccgg cggcgagcac cgtggtcaac atgttcaagt
4621 ccaacgggat caactccatg cggctgtacg ctcccgacca ggcggcgctg caggcggtcg
4681 gcggcacggg cgtgaacgtt gttgtgggcg cgcccaacga cgtgctctcc aacctcgccg
4741 ccagtcccgc agcggctgca tcgtgggtga ggagcaacat ccaggcgtac cccaaggtct
4801 ccttccggta cgtctgcgtg ggcaacgagg tcgccggcgg cgccacccag aaccttgtcc
4861 ccgccatgaa gaacgtgcag ggcgcgctgg cctccgccgg gctgggccac atcaaggtga
4921 ccacgtcggt gtcgcaggcc atcctgggcg tgtacagccc gccgtccgcc gggtccttca
4981 ccggagaggc ggacgcgttc atgggccccg tggtgcagtt ccttgcccgc accggcgcgc
5041 cgctcatggc caacatctac ccgtacctgg cctgggccta caacccgagc gccatggaca
5101 tgagctacgc gctcttcacc gcgtccggca ccgtggtcca ggacggctcc tacgggtacc
5161 agaacctgtt cgacaccacc gtggacgcct tctacacggc catggccaag cacggcggct
5221 ccaacgtgaa gctggtggtg tccgagagcg ggtggccgtc agccggcggc acggcggcga
5281 ccccggccaa cgccaggatc tacaaccagt acctcatcaa ccacgtcggg cgcggcaccc
5341 cccgccaccc gggcgccatc gagacctacg tcttctccat gttcaacgag aaccagaagg
5401 acaacggcgt ggagcagaac tgggggctct tctaccccaa catgcagcac gtctacccca
5461 tcagcttctg atgaggtagc agctacctag tgcccgtatg tccgtacgta cgcgcgcgcg
5521 tataagagcg tgtacgccgt acgtatgcgc acattatgta ttgtacaggg cttgggttgg
5581 gaacttggga tgcgaccgct gaggcagctc agatgcgtac gcgagtagta gtggcttgct
5641 atactagtgt accagtacgt atgattttcg atggaaggga agcatatgca aacgctcccc
5701 cttcctcgat tgatcatgca cttgatacgt acacgcatgt gtgcgtacct aggaactata
5761 ttgtagggtt caaaatttcg tcaaaacttg acgaaatttg tccaaaataa taaagtatga
5821 aatatatcgg cgaagaccga atattcctat atataatttt tccatgtgtc tataagccct
5881 gaccacacta acatataact ccaagatgtg caagtcggaa gaaacccaat taatcgcgtc
5941 ctacttaaga attcaaatga gaaaaatcac atggatgaca tgggcatcgt acatgagctc
6001 gtaaatggac atcagccgga cacgtgtcct cagaaaggga tgatgtagac gatcctgtcc
6061 ataccatttt ttcccctata ttgatctcaa atcgacctat atacttggat tattttgtca
6121 actaattcgg ctgcgcctta tccacgcgcg acgcagtagg tatcatacca tccgtgtgtt
6181 tttgtggata cgcatatccc gcggagacct acggatctcc tgcgtcaaca actctctgat
6241 ccgttgacct gcaggtcgac
//GenBank-Updates@genbank.bio.net (05/26/91)
LOCUS BLYGLUEND 4643 bp ds-DNA PLN 26-MAY-1991
DEFINITION Barley DNA for (1,3;1,4)-beta-glucanase.(EC number 3.2.1.73)
ACCESSION X56260
KEYWORDS endohydrolase; glucanase; lichenase.
SOURCE Hordeum vulgare DNA.
ORGANISM Hordeum vulgare
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
Commelinidae; Cyperales; Poaceae.
REFERENCE 1 (bases 1 to 4643)
AUTHORS Fincher,G.B.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Slakeski,N., Baulcombe,D.C., Devos,K.M., Ahluwalia,B., Doan,D.N.
and Fincher,G.B.
TITLE Structure and tissue-specific regulation of genes encoding barley
(1,3; 1,4)-beta-glucan endohydrolase
JOURNAL Mol. Gen. Genet. 224, 437-449 (1990)
STANDARD full staff_review
COMMENT *source: strain=NK 1558; developmental stage=7 day old seedlings;
*source: library=barley genomic;clone=lambda 14; **map: chromosome=
long arm of chromosome 1;
From EMBL entry HVGLUEND; dated 16-JAN-1991.
FEATURES Location/Qualifiers
mRNA join(474..619,3133..>4064)
sig_peptide join(546..619,3133..3143)
/note="putative"
/product="(1,3;1,4)-beta- glucanase"
sig_peptide join(3020..3077,3133..3143)
/note="putative alternative"
/product="(1,3;1,4)- beta-glucanase"
mRNA join(2826..3077,3133..4064)
/note="putative alternative"
/evidence=EXPERIMENTAL
CDS join(546..619,3133..4064)
/EC_number="3.2.1.73"
/note="syn. (1,3;1,4)-beta-glucanase"
/product="lichenase"
/codon_start=546
CDS join(2836..3077,3133..4064)
/EC_number="3.2.1.73"
/note="putative alternative"
/product="lichenase"
/codon_start=2836
exon 474..619
/number=1
exon 474..619
prim_transcript 474..>4643
intron 620..3132
/number=1
exon 2826..3077
/number=1
exon 2826..3077
/note="exon 1a"
prim_transcript 2836..>4643
/note="putative alternative"
intron 3078..3132
/number=1
/note="intron 1a"
exon 3133..4064
/number=2
exon 3133..>4064
/number=2
exon 3133..>4064
exon 3133..4064
exon 3133..4064
exon 3133..4064
/note="putative alternative"
mat_peptide 3144..4061
/EC_number="3.2.1.73"
/note="syn. (1,3;1,4)-beta-glucanase"
/product="lichenase"
/evidence=EXPERIMENTAL
polyA_signal 4399..4404
BASE COUNT 1166 a 1190 c 1110 g 1177 t
ORIGIN
1 cgttgtgtca attcttctta cgtaagttat gtcggattcc accgaacacg gtccttgcgt
61 gaaaaaaccg ccgccgaatg tcgttgagta agacgtgaac atatccgacg cggcgcgacc
121 catcattgac ctagaaactt cactttcatg gtacatcatg ggtggagtcc aaaattcaaa
181 ctattttttc aaaagttgtt tggtaccact atgaatgaat gaattatccc cttcccctac
241 ctgcaacaac aacctggtgt accggataac ctctgcccac caccagacac acactgtgag
301 aaggcggtga cgcatgcaaa ccggcctagg tagtcaatcg caacaggcta aataaatgtc
361 gctggaggcg ttgggcctgc gctcccgagt ggattggacc gaactatgtc tcctcggatc
421 ctatataagg ggcctgcacc ccgttgtggc ctcaccagaa aagaaacaac aagagctttt
481 acagagagcc ttggcatcac ccacccacac cctcaccctc caacgcagct agagagagaa
541 agagaatggc aggccaaggc gttgcctcca tgttggctct ggcattgctc ctcggagcct
601 tcgcctccat cccacaaagt gattcccctt ccttccctcc ctctctctct cttgaagtga
661 ttgggtgcag tttttttttt tcatgcaggg agtttctttg aagatagtaa tacgtacgtt
721 gatcttagct ttcattaaga gaagcattag ggagctagct aggtagccgg cggccatggt
781 gtacccatgc tcacataaac ctctgtggga cgtcacatag cataccttcc tctgtttagg
841 tgcatgtcgc tttcgttctg cgttttattc ttcccgcgcc atcagtcgca tggtaaagca
901 tgaacggccg gcgcccgccg gtctcattat ttacctcgat gcgtccatca atgcatggga
961 gcacagtaac ttttcaaaaa aaaaattcga aaactaaaca cggtaaattt tgtaaaaaaa
1021 atcgttttca ctcgctaaaa gaatcaggag tcttgggttt tgctgcggag tatacgtatc
1081 aaaatatatg caccgcacgc tccaagataa aagtgcacgt cagcacgtgt gatcggcctt
1141 cattgtgttt cttctatgga agaaaacgtt gcaagaagta ggtattccgc aggaaaatta
1201 atatcttaag aacaacaaaa atgatatttt ctagataaaa cattgaaaga ggaagtaagc
1261 agtgaaaacg atgcttgtag atagtttcaa ctgttttaac cataaattaa tctagttgat
1321 cgtacgcaaa agcatcaagc aaaagcttgc aaatgaagtc tcgaaatgtt tggtgaccat
1381 gcatgtcagt tagctagctg catcttttta caaagtcaga aggtttaact aacgacctac
1441 aagtagctag ctaggccagt gtctctttct ccagtgtcat gcatgctaat cacctagaaa
1501 gtttttcttc ttcttcctcc tcttccctgc atgcaaagct gtgcatgcat gtctcgacag
1561 tgtcgcccta gcactgtaga cacgagccct accacttaat tggtggtttg ttccctgatt
1621 aattcggtgc aactactgct gaaccacccg tcgctaacca tctttttctt cttcctttgg
1681 tccgcatctt gacgtttttt cagcgatgga cgatgccgta ccggccagtc cagtggtcct
1741 actcgtttca gctagccact gttccattct tttaagctta acgaagacga ttacaccagc
1801 tgctagctag ccagtaacta taactcaact gcagcctttg tgtagtcatc atatcgactt
1861 tgaaagtgca accggtggat ccatggtaca tgacgccatc gatcaccagg cactaactaa
1921 ccatgcataa cccaggtgtt ggttctgctg gcctttccaa gtttggtctg cctgaaaaga
1981 attgtgatat aataggattg taggttagtg tagtggtact agctagtagt tggcacttga
2041 tcggccgggc agcaagttaa gaagaggatt aagttgcgtg tacttatatg gagtactttg
2101 tcatgcacgt gagctagacc gtttattgga gcttagctgg gagcagccgg agggcatgca
2161 tgcatgcacg ccatggcgat ccacatcgat cgtatgtgga ctatccaacg gccgtgctgc
2221 ggacgttgga ccagaaactt tggtttgtcc tagcatgtat gtatgcatga ggtctctaca
2281 cccccctgta tttctaggct tcctgccaat tgccatctac gtgtggtcga cctccattct
2341 gccctacgat attcctggcc cgtttagttt aataaatcca gctagctagt gccgtgcaag
2401 tactacacaa tttatgccat accattgctt ggcgatggca ctcttaagtg tacacgtact
2461 gtaaaaatac atgtattctt tctagcataa ttgatgtacc aactatcaag tgtaacactt
2521 taatagagta gattggtgta gagttagttg gtaaaccaag caaatcaggg agggtaaaag
2581 attcactgca ctggaagcta gacaaagttc attccttcct ttaaggttat ctagttcctt
2641 tttctccttg ctcgtggtag agtagctagt agctagtgac aagtcggtca aggcgccggc
2701 cgtgaaaata gcaatgttcc tcggccgtgt gcgtgcatct gacaccaact cgtgactgta
2761 aaaaaaacaa tatttaagtg gtgcatcagc caccaaagta tatagagaga aagagataaa
2821 aaaatgtgca aaactagtta gctagaggct tacacgcatg catccatgca tgcgtacaag
2881 aatttcagtt aaacgtcctt ttcggggagt tagtattatc cctcgccaac aggtcaaaag
2941 gctctgtgtg catgtgtgtt catgcatcgc cgccattctt gcttattggt ttctttttta
3001 tattccatca catgccatca tgggagaatt ttttaatttt ctactatggc aatggaacag
3061 tgctactact ctacctggtg caaataattg attttgtgaa ggttaactaa ccgaggttat
3121 attacattgc aggcgtggag tccatcgggg tgtgctacgg catgagcgcc aacaatctgc
3181 cggcggcgag caccgtggtc aacatgttca agtccaacgg gatcaactcc atgcggctgt
3241 acgctcccga ccaggcggcg ctgcaggcgg tcggcggcac gggcgtgaac gttgttgtgg
3301 gcgcgcccaa cgacgtgctc tccaacctcg ccgccagtcc cgcagcggct gcatcgtggg
3361 tgaggagcaa catccaggcg taccccaagg tctccttccg gtatgtctgc gtgggcaacg
3421 aggtcgccgg cggcgccacc cagaaccttg tccccgccat gaagaacgtg cagggcgcgc
3481 tggcctccgc cgggctgggc cacatcaagg tgaccacgtc ggtgtcgcag gccatcctgg
3541 gggtgtacag cccgccgtcc gccgggtcct tcaccggaga ggcggacgcg ttcatgggcc
3601 ccgtggtgca gttccttgcc cgcaccggcg cgccgctcat ggccaacatc tacccgtacc
3661 tggcctgggc ctacaacccg agcgccatgg acatgagcta cgcgctcttc accgcctccg
3721 gcaccgtggt ccaggacggc tcctacgggt accagaacct gttcgacacc accgtggacg
3781 ccttctacac ggccatggcc aagcacggcg gctccaacgt gaagctggtg gtgtccgaga
3841 gcgggtggcc gtcagccggc ggcacggcgg cgaccccggc caacgccagg atctacaacc
3901 agtacctcat caaccacgtc gggcgcggca ccccccgcca cccgggcgcc atcgagacct
3961 acgtcttctc catgttcaac gagaaccaga aggacaacgg cgtggagcag aactgggggc
4021 tcttctaccc caacatgcag cacgtctacc ccatcagctt ctgatgaggt agcagctacc
4081 tagtgcccgt atgtccgtac gtacgcgcgc gcgtataaga gcgtgtacgc cgtacgtatg
4141 cgcacattat gtattgtaca gggcttgggt tgggaacttg ggatgcgacc gctgaggcag
4201 ctcagatgcg tacgcgagta gtggcttgct atactagtgt accagtacgt atgattttcg
4261 atggaaggga agcatatgca aacgctcccc cttcctcgat tgatcatgca cttgatacgt
4321 acacgcatgt gtgcgtacct aggaactata ttgtagggtt caaaatttcg tcaaaacttg
4381 acgaaatttg tccaaaataa taaagtatga aatatatcgg cgaagaccga atattcctat
4441 atataatttt tccatgtgtc tataagccct gaccacacta acatataact ccaagatgtg
4501 caagtcggaa gaaacccaat taatcgcgtc ctacttaaga ttcaaatgag aaaaatcaca
4561 tggatgacat gggcatcgta catgagctcg taaatggaca ccagccggac acgtgtcctc
4621 agaaagggat gatgtagacg atc
//