GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS BLYBDG 6260 bp ds-DNA PLN 18-MAY-1991 DEFINITION Barley DNA for (1-3,1-4)-beta-D-glucanase (EC 3.2.1.73) ACCESSION X52572 KEYWORDS (1-3,1-4)-beta-D-glucanase; glucanase. SOURCE Hordeum vulgare DNA. ORGANISM Hordeum vulgare Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida; Commelinidae; Cyperales; Poaceae. REFERENCE 1 (bases 1 to 6260) AUTHORS Litts,J.C. JOURNAL Unpublished (1990) STANDARD full automatic REFERENCE 2 (bases 1 to 6260) AUTHORS Litts,J.C., Simmons,C.R., Karrer,E.E., Huang,N. and Rodriguez,R.L. TITLE The isolation and characterization of a barley 1,3-1,4-beta- glucanase gene JOURNAL Eur. J. Biochem. 194, 831-838 (1990) STANDARD full automatic COMMENT *source: strain=Klages; clone=lambda HV29; From EMBL entry HVBDG; dated 21-FEB-1991. FEATURES Location/Qualifiers promoter 1854..1860 /note="put. TATA box" precursor_RNA 1886..>1885 /note="primary transcript" mRNA 1886..2049 /note="exon 1" CDS 1977..2049 /note="(1-3,1-4)-beta-D-glucanase (AA 1-24) (2049 is 1st base in codon)" /codon_start=1977 intron 2050..4539 /note="intron I" mRNA 4540..>4539 /note="exon 2" CDS 4540..5468 /note="(1-3,1-4)-beta-D-glucanase (AA 25-334) (4540 is 2nd base in codon)" /codon_start=4540 BASE COUNT 1667 a 1550 c 1414 g 1629 t ORIGIN 1 ggatccggta ttctcaacca tatcggataa gtacaatttt tggtttgtat cagataaata 61 ggtttggact ttcaggtttt ggtaagcatg ggagtctgta atcctggtgg taagctgctg 121 atcctccctt tttctgaaag aaaaaaaaaa ggttactaac cctttgaatg aatcaggaca 181 aactaagcct ctcaagtgga aattcggaac caatgatgtg ccagaaacta tcttggctac 241 atgcacggta cgaattggca gcatcatcat aactacaact tgatcaatat acaactcact 301 acaaaacaca tgtctcaaga agaccatttt agcatatagg accaattcag tacatgtgcc 361 aaaagagggc agcccggctt gcatctgtca aaaagttggg ctgctaagcc accaatagga 421 aatttctcat caaccagccg tatatgcatg tgtgttaacc gaaaccaata tgcccaagca 481 gaccacctgc atgctagcta atcctagaca cgaccggctc ttaaatacaa ttggtccggt 541 cgaataattc gttcaccgaa atgagtcccg agtcatcgta cagtttttat attcgtatta 601 actgtaatct acacatttgg tgcacctaat attatttcgt cattagcact tatactacaa 661 gctagagagc agttgcatga ccggccggac tctgctgata gcaacaacat actgtgtgta 721 aaagaactat caacggccgg atctggatat cccacctgat caataggatt gggagggaga 781 gacgtacgta cttagtttgt tgtcggggtt attacaacac gtatggtgcc acgagggaaa 841 accatgcatg cgttgcacca aaagctcaca tgtatggtgc gcttagacaa gccgtggtgt 901 tgtgttccta tggcaattcc taataaaggt cttctttgct cttttcaatt gcaaggccga 961 tgaactcaat accttctctt gtgagacaac aagtccaaaa gttaagtggg tctggtcttg 1021 tttgacaaag tataaagaca tgcttaacta aagtggtttg agtaattcta tgattctttt 1081 gacaaaaatc ttaattaatt aggtacacca aaaacacaaa tgttcttatg ataaacaaaa 1141 ttgttggttt cttgcaatat aataacaaaa caatggcatg taaaaatacg agcatgtact 1201 acatatatac cggtatgaga cggtaaaagg tagagagacc aaaggcttct gatgtactcc 1261 aaatctcttt aaattgaccc cgaatatcca ttactaaaat ataatttcat ttgtatacat 1321 gtcacttcac gataagaaac aacaaagccg acaaaaccgt tgcgcccggt tctttcctac 1381 gtaagtcatg tcaacttcaa agataaaaaa aaaatcattg ccaacaagtc tccgttgtgt 1441 caattcttct tacgtaagtt atgtcggatt ccaccgaaca cggtccttgc gtgaaaaaac 1501 cgccgccgaa tgtcgttgag taagacgtga acatacccga cgccgcgcga cccatcattg 1561 acctagaaac ttcactttca tggtacatca tgggtggagt ccaaaattca aactattttt 1621 tcaaaagttg tttggtacca ctatgaatga atgaattatc cccttcccct acctgcaaca 1681 acaacctggt gtaccggata acctctgccc accaccagac acacactgtg agaaggcggt 1741 gacgcatgca aaccagccta ggtagtcaat cgcaacaggc taaataaatg tcgctggagg 1801 cgttgggcct gcgctcccga gtggattgga ccgaactatg tctcctcgga tcctatataa 1861 ggggcctgca ccccgttgtg gcctcaccag aaaagaaaca acaagagctt tacagagagc 1921 cttggcatca cccacccaca ccctcaccct ccaacgcagc tagagagaga aagagaatgg 1981 caggccaagg cgttgcctcc atgttggctc tggcattgct cctcggagcc ttcgcctcca 2041 tcccacaaag tgattcccct tccttccctc cctctctctc tcttgaagtg attgggtgca 2101 gttttttttt tcatgcaggg agtttctttg aagatagtaa tacgtacgtt gatcttagct 2161 ttcattaaga gaagcattag ggagctagct aggtagccgg cggccatggt gtacccatgc 2221 tcacataaac ctctctccca cagacgtcac atagcatacc ttcctctgtt taggtgcatg 2281 tcgctttcgt tctgcgtttt attcttcccg cgccatcagt cgcatggtaa agcatgaacg 2341 gccggcgccc gccggtctca ttatctacct cgatgcgtcc atcaatgcat gggagcacag 2401 taacttttca aaaaaaaaat cgaaaactaa acacggtaaa ttttgtaaaa aaaatcgttt 2461 tcactcgcta aaagaatcag gagtcttggg ttttgctgcg gagtatacgt atcaaaatat 2521 atgcaccgca cgctccaaga taaaagtgca cgtcagcacg tgtgatcggc cttcattgtg 2581 tttcttctat ggaagaaaac gttgcaagaa gtaggtattc cgcaggaaaa ttaatatctt 2641 aagaacaaca aaaatgatac tttctagata aaacattgaa agaggaagta agcagtgaaa 2701 acgatgcttg tagatagttt caactgtttt aaccataaat taatctagtt gatcgtacgc 2761 aaaagcttgc aaatgaagtc tcgaaatgtt tggtgaccat gcatgtcagt tagctagctg 2821 catcttttta caaagtcaga aggtttaact aacgacctac aagtagctag ctaggccagt 2881 gtctctttct ccagtgtcat gcatgctaat cacctagaaa gtttttcttc ttcttcctcc 2941 tcttccctgc atgcaaagct gtgcatgcat gtctcgacag tgtcgcccta gcactgtaga 3001 cacgagccct accacttaat tggtggtttg ttccctgatt aattcggtgc aactactgct 3061 gaaccacccg tcgctaacca tctttttctt cttcctttgg tccgcatctt gacgtttttt 3121 cagcgatgga cgatgccgta ccggccagtc cagtggtcct actcgtttca gctagccact 3181 gttccattct tttaagctta acgaagacga ttacaccagc tgctagctag ccagtaacta 3241 taactcaatt gcagcctttg tgtagtcatc atatcgactt tgaaagtgca accggtggat 3301 ccatggtaca tgacgccatc gatcactagg cactaactaa ccatgcataa cccaggtgtt 3361 ggttctgctg gcctttccaa gtttggtctg cctgaaaaga attgtgatat aataggattg 3421 taggttagtg tagtggtact agccagtagt tggcacttga tcggccgggc agcaagttaa 3481 gaagaggatt aagttgcgtg tacttatatg gagtactttg tcatgcacgt gagctagacc 3541 gtttattgga gcttagctgg gagcagccgg agggcatgca tgcacgccat ggcgatccac 3601 atcgatcgta tgtggactat ccaacggccg tgctgcggac gttggaccag aaactttggt 3661 ttgtcctagc atgtatgtat gcatgaggtc tctacacccc cctgtatttc taggcttcct 3721 gccaattgcc atctacgtgt ggtcgacctc cattctgccc ctacgatatt cctggcccgt 3781 tttgtttaat aaatccagct agctagtgcc gtgcaagtac tacacaattt atgccatacc 3841 attgattggc gatggcactc ttaagtgtac acgtactgta aaaatacatg tattctttct 3901 agcataattg atgtaccaac tatcaagtgt aacactttaa tagagtagat tggtgtagag 3961 ttagttggta aaccaagcaa atcagggagg gtaaaagatt cactgcactg gaagctagtc 4021 aaagttcatt ccttccttta aggttatcta gttccttttt ctccttgctc gtggtagagt 4081 agctagtagc tagtgacaag tcggtcaagg cgccggccgt gaaaatagca atgttcctcg 4141 gccgtgtgcg tgcatctgac accaactcgt gactgtaaca aaaacaatat ataagtgctg 4201 catcagccac caaagtctag agagaaagag ataaaaaaaa tgcgcaaagc tagaggctta 4261 cacgcatgca tccatgcatg cgtacaagat ttcagttaaa cgtccttttc ggggagttag 4321 tattatccct cgccaacagg tcaaaaggct ctgtgtgcat gtgtgttcat gcatcgccgc 4381 cattcttgct tattggtttc ttttttatat tccatcacat gccatcatgg gagaattttt 4441 taattttcta ctatggcaat ggaacagtgc tactactcta cctggtgtaa ataattgatt 4501 ttgtgaaggt taactaaccg aggttatatt acattgcagg cgtggagtcc atcggggtgt 4561 gctacggcat gagcgccaac aatctgccgg cggcgagcac cgtggtcaac atgttcaagt 4621 ccaacgggat caactccatg cggctgtacg ctcccgacca ggcggcgctg caggcggtcg 4681 gcggcacggg cgtgaacgtt gttgtgggcg cgcccaacga cgtgctctcc aacctcgccg 4741 ccagtcccgc agcggctgca tcgtgggtga ggagcaacat ccaggcgtac cccaaggtct 4801 ccttccggta cgtctgcgtg ggcaacgagg tcgccggcgg cgccacccag aaccttgtcc 4861 ccgccatgaa gaacgtgcag ggcgcgctgg cctccgccgg gctgggccac atcaaggtga 4921 ccacgtcggt gtcgcaggcc atcctgggcg tgtacagccc gccgtccgcc gggtccttca 4981 ccggagaggc ggacgcgttc atgggccccg tggtgcagtt ccttgcccgc accggcgcgc 5041 cgctcatggc caacatctac ccgtacctgg cctgggccta caacccgagc gccatggaca 5101 tgagctacgc gctcttcacc gcgtccggca ccgtggtcca ggacggctcc tacgggtacc 5161 agaacctgtt cgacaccacc gtggacgcct tctacacggc catggccaag cacggcggct 5221 ccaacgtgaa gctggtggtg tccgagagcg ggtggccgtc agccggcggc acggcggcga 5281 ccccggccaa cgccaggatc tacaaccagt acctcatcaa ccacgtcggg cgcggcaccc 5341 cccgccaccc gggcgccatc gagacctacg tcttctccat gttcaacgag aaccagaagg 5401 acaacggcgt ggagcagaac tgggggctct tctaccccaa catgcagcac gtctacccca 5461 tcagcttctg atgaggtagc agctacctag tgcccgtatg tccgtacgta cgcgcgcgcg 5521 tataagagcg tgtacgccgt acgtatgcgc acattatgta ttgtacaggg cttgggttgg 5581 gaacttggga tgcgaccgct gaggcagctc agatgcgtac gcgagtagta gtggcttgct 5641 atactagtgt accagtacgt atgattttcg atggaaggga agcatatgca aacgctcccc 5701 cttcctcgat tgatcatgca cttgatacgt acacgcatgt gtgcgtacct aggaactata 5761 ttgtagggtt caaaatttcg tcaaaacttg acgaaatttg tccaaaataa taaagtatga 5821 aatatatcgg cgaagaccga atattcctat atataatttt tccatgtgtc tataagccct 5881 gaccacacta acatataact ccaagatgtg caagtcggaa gaaacccaat taatcgcgtc 5941 ctacttaaga attcaaatga gaaaaatcac atggatgaca tgggcatcgt acatgagctc 6001 gtaaatggac atcagccgga cacgtgtcct cagaaaggga tgatgtagac gatcctgtcc 6061 ataccatttt ttcccctata ttgatctcaa atcgacctat atacttggat tattttgtca 6121 actaattcgg ctgcgcctta tccacgcgcg acgcagtagg tatcatacca tccgtgtgtt 6181 tttgtggata cgcatatccc gcggagacct acggatctcc tgcgtcaaca actctctgat 6241 ccgttgacct gcaggtcgac //
GenBank-Updates@genbank.bio.net (05/26/91)
LOCUS BLYGLUEND 4643 bp ds-DNA PLN 26-MAY-1991 DEFINITION Barley DNA for (1,3;1,4)-beta-glucanase.(EC number 3.2.1.73) ACCESSION X56260 KEYWORDS endohydrolase; glucanase; lichenase. SOURCE Hordeum vulgare DNA. ORGANISM Hordeum vulgare Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida; Commelinidae; Cyperales; Poaceae. REFERENCE 1 (bases 1 to 4643) AUTHORS Fincher,G.B. JOURNAL Unpublished (1990) STANDARD full automatic REFERENCE 2 (sites) AUTHORS Slakeski,N., Baulcombe,D.C., Devos,K.M., Ahluwalia,B., Doan,D.N. and Fincher,G.B. TITLE Structure and tissue-specific regulation of genes encoding barley (1,3; 1,4)-beta-glucan endohydrolase JOURNAL Mol. Gen. Genet. 224, 437-449 (1990) STANDARD full staff_review COMMENT *source: strain=NK 1558; developmental stage=7 day old seedlings; *source: library=barley genomic;clone=lambda 14; **map: chromosome= long arm of chromosome 1; From EMBL entry HVGLUEND; dated 16-JAN-1991. FEATURES Location/Qualifiers mRNA join(474..619,3133..>4064) sig_peptide join(546..619,3133..3143) /note="putative" /product="(1,3;1,4)-beta- glucanase" sig_peptide join(3020..3077,3133..3143) /note="putative alternative" /product="(1,3;1,4)- beta-glucanase" mRNA join(2826..3077,3133..4064) /note="putative alternative" /evidence=EXPERIMENTAL CDS join(546..619,3133..4064) /EC_number="3.2.1.73" /note="syn. (1,3;1,4)-beta-glucanase" /product="lichenase" /codon_start=546 CDS join(2836..3077,3133..4064) /EC_number="3.2.1.73" /note="putative alternative" /product="lichenase" /codon_start=2836 exon 474..619 /number=1 exon 474..619 prim_transcript 474..>4643 intron 620..3132 /number=1 exon 2826..3077 /number=1 exon 2826..3077 /note="exon 1a" prim_transcript 2836..>4643 /note="putative alternative" intron 3078..3132 /number=1 /note="intron 1a" exon 3133..4064 /number=2 exon 3133..>4064 /number=2 exon 3133..>4064 exon 3133..4064 exon 3133..4064 exon 3133..4064 /note="putative alternative" mat_peptide 3144..4061 /EC_number="3.2.1.73" /note="syn. (1,3;1,4)-beta-glucanase" /product="lichenase" /evidence=EXPERIMENTAL polyA_signal 4399..4404 BASE COUNT 1166 a 1190 c 1110 g 1177 t ORIGIN 1 cgttgtgtca attcttctta cgtaagttat gtcggattcc accgaacacg gtccttgcgt 61 gaaaaaaccg ccgccgaatg tcgttgagta agacgtgaac atatccgacg cggcgcgacc 121 catcattgac ctagaaactt cactttcatg gtacatcatg ggtggagtcc aaaattcaaa 181 ctattttttc aaaagttgtt tggtaccact atgaatgaat gaattatccc cttcccctac 241 ctgcaacaac aacctggtgt accggataac ctctgcccac caccagacac acactgtgag 301 aaggcggtga cgcatgcaaa ccggcctagg tagtcaatcg caacaggcta aataaatgtc 361 gctggaggcg ttgggcctgc gctcccgagt ggattggacc gaactatgtc tcctcggatc 421 ctatataagg ggcctgcacc ccgttgtggc ctcaccagaa aagaaacaac aagagctttt 481 acagagagcc ttggcatcac ccacccacac cctcaccctc caacgcagct agagagagaa 541 agagaatggc aggccaaggc gttgcctcca tgttggctct ggcattgctc ctcggagcct 601 tcgcctccat cccacaaagt gattcccctt ccttccctcc ctctctctct cttgaagtga 661 ttgggtgcag tttttttttt tcatgcaggg agtttctttg aagatagtaa tacgtacgtt 721 gatcttagct ttcattaaga gaagcattag ggagctagct aggtagccgg cggccatggt 781 gtacccatgc tcacataaac ctctgtggga cgtcacatag cataccttcc tctgtttagg 841 tgcatgtcgc tttcgttctg cgttttattc ttcccgcgcc atcagtcgca tggtaaagca 901 tgaacggccg gcgcccgccg gtctcattat ttacctcgat gcgtccatca atgcatggga 961 gcacagtaac ttttcaaaaa aaaaattcga aaactaaaca cggtaaattt tgtaaaaaaa 1021 atcgttttca ctcgctaaaa gaatcaggag tcttgggttt tgctgcggag tatacgtatc 1081 aaaatatatg caccgcacgc tccaagataa aagtgcacgt cagcacgtgt gatcggcctt 1141 cattgtgttt cttctatgga agaaaacgtt gcaagaagta ggtattccgc aggaaaatta 1201 atatcttaag aacaacaaaa atgatatttt ctagataaaa cattgaaaga ggaagtaagc 1261 agtgaaaacg atgcttgtag atagtttcaa ctgttttaac cataaattaa tctagttgat 1321 cgtacgcaaa agcatcaagc aaaagcttgc aaatgaagtc tcgaaatgtt tggtgaccat 1381 gcatgtcagt tagctagctg catcttttta caaagtcaga aggtttaact aacgacctac 1441 aagtagctag ctaggccagt gtctctttct ccagtgtcat gcatgctaat cacctagaaa 1501 gtttttcttc ttcttcctcc tcttccctgc atgcaaagct gtgcatgcat gtctcgacag 1561 tgtcgcccta gcactgtaga cacgagccct accacttaat tggtggtttg ttccctgatt 1621 aattcggtgc aactactgct gaaccacccg tcgctaacca tctttttctt cttcctttgg 1681 tccgcatctt gacgtttttt cagcgatgga cgatgccgta ccggccagtc cagtggtcct 1741 actcgtttca gctagccact gttccattct tttaagctta acgaagacga ttacaccagc 1801 tgctagctag ccagtaacta taactcaact gcagcctttg tgtagtcatc atatcgactt 1861 tgaaagtgca accggtggat ccatggtaca tgacgccatc gatcaccagg cactaactaa 1921 ccatgcataa cccaggtgtt ggttctgctg gcctttccaa gtttggtctg cctgaaaaga 1981 attgtgatat aataggattg taggttagtg tagtggtact agctagtagt tggcacttga 2041 tcggccgggc agcaagttaa gaagaggatt aagttgcgtg tacttatatg gagtactttg 2101 tcatgcacgt gagctagacc gtttattgga gcttagctgg gagcagccgg agggcatgca 2161 tgcatgcacg ccatggcgat ccacatcgat cgtatgtgga ctatccaacg gccgtgctgc 2221 ggacgttgga ccagaaactt tggtttgtcc tagcatgtat gtatgcatga ggtctctaca 2281 cccccctgta tttctaggct tcctgccaat tgccatctac gtgtggtcga cctccattct 2341 gccctacgat attcctggcc cgtttagttt aataaatcca gctagctagt gccgtgcaag 2401 tactacacaa tttatgccat accattgctt ggcgatggca ctcttaagtg tacacgtact 2461 gtaaaaatac atgtattctt tctagcataa ttgatgtacc aactatcaag tgtaacactt 2521 taatagagta gattggtgta gagttagttg gtaaaccaag caaatcaggg agggtaaaag 2581 attcactgca ctggaagcta gacaaagttc attccttcct ttaaggttat ctagttcctt 2641 tttctccttg ctcgtggtag agtagctagt agctagtgac aagtcggtca aggcgccggc 2701 cgtgaaaata gcaatgttcc tcggccgtgt gcgtgcatct gacaccaact cgtgactgta 2761 aaaaaaacaa tatttaagtg gtgcatcagc caccaaagta tatagagaga aagagataaa 2821 aaaatgtgca aaactagtta gctagaggct tacacgcatg catccatgca tgcgtacaag 2881 aatttcagtt aaacgtcctt ttcggggagt tagtattatc cctcgccaac aggtcaaaag 2941 gctctgtgtg catgtgtgtt catgcatcgc cgccattctt gcttattggt ttctttttta 3001 tattccatca catgccatca tgggagaatt ttttaatttt ctactatggc aatggaacag 3061 tgctactact ctacctggtg caaataattg attttgtgaa ggttaactaa ccgaggttat 3121 attacattgc aggcgtggag tccatcgggg tgtgctacgg catgagcgcc aacaatctgc 3181 cggcggcgag caccgtggtc aacatgttca agtccaacgg gatcaactcc atgcggctgt 3241 acgctcccga ccaggcggcg ctgcaggcgg tcggcggcac gggcgtgaac gttgttgtgg 3301 gcgcgcccaa cgacgtgctc tccaacctcg ccgccagtcc cgcagcggct gcatcgtggg 3361 tgaggagcaa catccaggcg taccccaagg tctccttccg gtatgtctgc gtgggcaacg 3421 aggtcgccgg cggcgccacc cagaaccttg tccccgccat gaagaacgtg cagggcgcgc 3481 tggcctccgc cgggctgggc cacatcaagg tgaccacgtc ggtgtcgcag gccatcctgg 3541 gggtgtacag cccgccgtcc gccgggtcct tcaccggaga ggcggacgcg ttcatgggcc 3601 ccgtggtgca gttccttgcc cgcaccggcg cgccgctcat ggccaacatc tacccgtacc 3661 tggcctgggc ctacaacccg agcgccatgg acatgagcta cgcgctcttc accgcctccg 3721 gcaccgtggt ccaggacggc tcctacgggt accagaacct gttcgacacc accgtggacg 3781 ccttctacac ggccatggcc aagcacggcg gctccaacgt gaagctggtg gtgtccgaga 3841 gcgggtggcc gtcagccggc ggcacggcgg cgaccccggc caacgccagg atctacaacc 3901 agtacctcat caaccacgtc gggcgcggca ccccccgcca cccgggcgcc atcgagacct 3961 acgtcttctc catgttcaac gagaaccaga aggacaacgg cgtggagcag aactgggggc 4021 tcttctaccc caacatgcag cacgtctacc ccatcagctt ctgatgaggt agcagctacc 4081 tagtgcccgt atgtccgtac gtacgcgcgc gcgtataaga gcgtgtacgc cgtacgtatg 4141 cgcacattat gtattgtaca gggcttgggt tgggaacttg ggatgcgacc gctgaggcag 4201 ctcagatgcg tacgcgagta gtggcttgct atactagtgt accagtacgt atgattttcg 4261 atggaaggga agcatatgca aacgctcccc cttcctcgat tgatcatgca cttgatacgt 4321 acacgcatgt gtgcgtacct aggaactata ttgtagggtt caaaatttcg tcaaaacttg 4381 acgaaatttg tccaaaataa taaagtatga aatatatcgg cgaagaccga atattcctat 4441 atataatttt tccatgtgtc tataagccct gaccacacta acatataact ccaagatgtg 4501 caagtcggaa gaaacccaat taatcgcgtc ctacttaaga ttcaaatgag aaaaatcaca 4561 tggatgacat gggcatcgta catgagctcg taaatggaca ccagccggac acgtgtcctc 4621 agaaagggat gatgtagacg atc //