GenBank-Updates@genbank.bio.net (05/26/91)
LOCUS TOMNIA 5309 bp ds-DNA PLN 26-MAY-1991
DEFINITION Lycopersicon esculentum nia gene for nitrate reductase (EC 1.6.6.1)
ACCESSION X14060
KEYWORDS nia gene; nitrate reductase.
SOURCE Lycopersicon esculentum DNA.
ORGANISM Lycopersicon esculentum
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Asteridae; Solanales; Solanaceae.
REFERENCE 1 (bases 1 to 5309)
AUTHORS Rouze,P.
JOURNAL Unpublished (1989)
STANDARD full automatic
REFERENCE 2 (bases 1 to 5309)
AUTHORS Daniel-Vedele,F., Dorbe,M.F., Caboche,M. and Rouze,P.
TITLE Cloning and analysis of the tomato nitrate reductase-encoding gene:
protein domain structure and amino acid homologies in higher plants
JOURNAL Gene 85, 371-380 (1989)
STANDARD full automatic
COMMENT SWISS-PROT; P17570; NIA$LYCES.
*source: strain=Manapal; tissue=leaf;
From EMBL entry LENIA; dated 03-MAY-1990.
FEATURES Location/Qualifiers
promoter 365..368
/note="put. CAAT-box"
promoter 438..445
/note="put. TATA-box"
mRNA 471..1595
/note="exon 1"
CDS 569..1595
/note="nitrate reductase (AA 1-342) (1595 is 1st base in
codon)"
/codon_start=569
intron 1596..1669
/note="intron I"
mRNA 1670..1810
/note="exon 2"
CDS 1670..1810
/note="nitrate reductase (AA 343-389) (1670 is 2nd base in
codon) (1810 is 1st base in codon)"
/codon_start=1670
intron 1811..2657
/note="intron II"
mRNA 2658..2890
/note="exon 3"
CDS 2658..2890
/note="nitrate reductase (AA 390-467) (2658 is 2nd base in
codon)"
/codon_start=2658
intron 2891..3326
/note="intron III"
mRNA 3327..>3326
/note="exon 4"
CDS 3327..4658
/note="nitrate reductase (AA 468-911)"
/codon_start=3327
misc_feature 4703..4708
/note="pot. polyA signal"
BASE COUNT 1675 a 905 c 1004 g 1725 t
ORIGIN
1 tacgatgaaa aatacacctt aaaatgttag tcgaagtttt tgtaatttga ctctgaaaat
61 agaaaccacg acacttaata gtgaacggag agagtaattg atataatttc tattttagag
121 tcaaactata aaatttaacc aacatcttgt aatttagtag tatttttcat atataatttt
181 tgaatatcta aaatttttaa ctttgaaagt taacgtaaca tgacaaataa aatcaataaa
241 tgaggaaaat aattttcatc aaatttaaaa ttgtttttaa tatttgaacg ataaaattgt
301 aaatcatata gacgaatttt tattattttt gctgatgatg tcacaaactt ttgtaatcaa
361 aattcaattt tggtgtcgat tttttgggtc ctacttatgt acagtaaata tggggttgag
421 atagttcgta acggttctat ataaacacaa ctctccgcac tcacaatatt cgttccaaaa
481 ccatacaaag aaccatcaaa atctaattag aaatctcttt tttcttcttc caaattccct
541 cattcataat tttattttaa aaaaatcaat ggcggcatct gtggaaaaca gacagtatac
601 tcaccttgaa ccgggtttat caggcgtagg ccgtactttc aagcctaggc ctgattcccc
661 ggttcgtggt tgcaacttcc ctccttcatc caaccatgaa ctccctttcc aaaaaaaaca
721 aaatccccca atttaccttg attattcgtc tagtgaagac gaggatgacg atgacgaaaa
781 aaatgaatac gttcaaatga tcaaaaaagg taaaactgaa ttagaaccat caattcatga
841 tactagagat gaaggtaccg ctgataattg gatcgaacga aacttttcct taatacgtct
901 caccggtaaa cacccattca actccgaacc acctttatct cgccttatgc atcacgggtt
961 catcactccc gtaccacttc attacgtccg taaccacggc ccagtcccca aagcatcctg
1021 gtctgactgg actgtggaag ttacagggct ggtaaaacga ccaatgaaat tcacaatgga
1081 tcaattagtt aacgaattcc cttcacgtga attccctgtc acacttgtgt gcgcaggcaa
1141 tcgtcgtaaa gagcagaata tggtgaagca gacaattggt ttcaattggg gtgctgctgc
1201 cgtttcaacc accgtatggc gcggagtacc tctccgcgcc ctgttgaaac ggtgcggtgt
1261 tcagagtaag aaaaaaggcg cgcttaatgt ctgtttcgaa ggttccgatg ttttgcctgg
1321 aggtggtggt tcaaagtacg gaacgagtat aaagaaggaa ttcgccatgg atccatctcg
1381 tgatattatt gtagcttaca tgcaaaacgg agaaatgttg tcaccggatc atggttttcc
1441 ggtaaggatg attatccccg gattcatcgg tggaagaatg gtgaaatggt taaagaggat
1501 tgtggtcact acacaagaat cggaaagcta ttatcattac aaggacaata gagtcctccc
1561 tccacacgtt gacgcggaac ttgccaacgc ggaaggtacg atatgaataa taattagcat
1621 ctattttaga tcaaagttat ttattatact caagttgtca tttgtttagc ttggtggtac
1681 aaaccagagt acatcatcaa tgagctcaac ataaactctg tcattacaac tccgtgccat
1741 gaagaaattt tgcccatcaa tgcgtggact actcagagac cttacacgtt gagaggctat
1801 gcttattctg gttagttatt tttctctctg atttgctgaa ttttcttagt actgtcgaat
1861 ttcatcaact gtttgtttta attatttcgt acctcttgtt ttcagctaat ttcaacctca
1921 ccctcttatc tcatattatc atacaaattt taagatttca tgaaaaaagt atattagtac
1981 gaatattttg tatgttgttc gaacttttta gaaatatcaa taaatatata tcgtatttgt
2041 cgaaaatact gtatttttaa agagtctgca atttaggata gtaaaggaaa aataggggtg
2101 agtggaggtg gatgcatgaa ctttttgatg gtgtcatcaa cttgtttggt tatgtaaaca
2161 gaatagcaag aaaaagacaa tatagttgga tttatttaat ggtttacaga aaaacctggg
2221 gagcttttcc tagttctgaa gagtcggtct aaaaataata tataggtaac aaaatttaat
2281 tgcctacaaa gacagaatct tttttgactg ctttagttcc tgcatcttag gctgcctcaa
2341 caacattata ataagttaaa ttattattat tatataatac atcattgtgt ttgtattcaa
2401 tttgaaaact ttcaagtggt cactttatat agatctagtc aatattattt taatttaagc
2461 ctaggtataa ggacttttat ctttttctaa tcaacgtgcc taacttgttg caattttgat
2521 tcaaatacaa gtttgaccct tcaagtgggg aaagtgttac attttttaat tgaatagtca
2581 gttacatgtt taaattaagc tattagacac gattacagtt gaatttatga attgacgaga
2641 tggtgattgt gtaataggtg gaggtaaaaa ggtaactcga gtggaagtga ctttggatgg
2701 aggagagaca tggagtgtgt gtacacttga tcacccagag aagccaacaa agtatggcaa
2761 gtactggtgt tggtgctttt ggtcactcga ggttgaggtg cttgacttgc ttagtgctaa
2821 agaaattgct gtacgagcta ccgatgagac cctcaacact caacccgaga agcttatttg
2881 gaacgtcatg gtaaattcac atttaacttt ttacaacttc tttaaaatta atggcgtaaa
2941 gtcaaatata tacatgtaac tagtccttga tgaaaagtaa ttttcagtcc ttttcaactt
3001 ttcttttatt tatgattact ttaccatatg cagattgata gagcatgtga tttcacataa
3061 aaacaaattt ttttcattgg tttaagtttc agattctttt tttctgatct atgtggtcca
3121 tggatgatta atttaaacca cagaaagtca tgaaaattcc tattatgcgt aagcagcagc
3181 gtgatcacat gaatatgtca cgtgctaggc ctcttattat aatttaagtt gatcgtgtta
3241 ctgatggagg acggttacta gtaagtaact ggtaatgttg ttatacattt gtctaatttt
3301 atggtgatgg atgttgtaat gttcagggaa tgatgaacaa ttgttggttt cgagtgaaga
3361 tgaatgtgtg caaacctcac aagggagaga ttggtatagt gtttgagcat ccgactcaac
3421 ctggaaatca atcgggtgga tggatggcaa aggagagaca cttggagata tcagcagtgg
3481 ctcctccaac actaaagaag agtatatcaa ctcctttcat gaacacagct tcgaagatgt
3541 attccatgtc cgaggtgagg aaacacaact cttcagactc tgcttggatc atagtccatg
3601 gacatatcta cgatgcctca cgtttcttga aagaccatcc cggtggtgtt gacagcattc
3661 tgatcaatgc tggaactgat tgtactgagg aatttgatgc aattcattct gataaggcta
3721 agaagctatt ggaggacttt aggattggtg aactcataac tactggttac acgtctgatt
3781 cgtctccaaa cagttctgtc catggatcct cttcgatcag tagcttctta gcacctatta
3841 aggagcttgt tcaaacacca acaaggagtg tagctctcat cccaagggaa aaaatccctt
3901 gcaaactcgt cgacaagcaa tccatctccc atgatgttag gaaattcaaa tttgcattac
3961 cctctgagga tcaagtctta gggttacctg ttggcaaaca catattcctc tgtgccacag
4021 ttgatgacaa actctgtatg cgtgcctaca cgcctactag cacagtcgat gaagtagggt
4081 tcttcgagtt ggttgtcaag atctacttca aaggtgttca ccctaaattc cctaatggag
4141 gtcaaatgtc acaacatctt gattctctcc caataggtgc attccttgac gttaaaggtc
4201 cattaggtca cattgaatac caaggtaagg gtaatttctt agtccatggt aaacaaaagt
4261 ttgccaagaa gttagctatg atagcgggtg gaacaggtat aactccagta tatcaagtaa
4321 tgcaatcaat attgaaagat cctgaagacg atacagagat gtatgtggtg tatgcaaaca
4381 gaacggagga tgatattttg ctcaaagacg aacttgatgc atgggcagag caagttccaa
4441 atagggttaa agtatggtat gtcgttcaag aatccattac acaaggatgg aagtatagta
4501 caggattcgt tacagaatcg attcttagag aacatatacc tgaaccatct catacaacat
4561 tggcattagc atgtggacca cctccaatga tacaatttgc tattaatcca aacttggaga
4621 aaatgggata tgacattaag gaggaactat tggtgttcta aattggatgg tgatgatgat
4681 agatgatata tctctttgga ggaataaatt cttttgtatt ttcagttgta catattgtgt
4741 ttttgtttat catcaaaatg tactacttct tgtagttctt acattttaat tttctactca
4801 actttaggta taaaatgttg tactgtgtac tagtatgtgt tatgtcaaag ttaagattgt
4861 attcatcatg gaattacagt actttgttgt cacaagtctt gttgtatata tttttaccaa
4921 attaatgtgt atatatatat agtaaattga aacatttgtg tgtgtgttta tttattttcc
4981 tctcaactct aaaactacaa ttttttttgc tcttcattca atattaatgt tgtaattagc
5041 actccattac aagcctaaag agtaaaggat agtggatcat ctactctaat atggaataag
5101 aaaatcgaca ttggtatatc gttgaaaata taattaaata ataaaaatat gtttttttca
5161 aaagtttaag tctttagatc acatggtcac acaattcaat atgatatcag aactgacaga
5221 agttttgaaa tcgaattatc agtttagtcg agtaatctta ttttgcatat tttatatata
5281 cctatagtgt taaacttcac gaaaagaca
//