GenBank-Updates@genbank.bio.net (05/26/91)
LOCUS RIC4CL 5225 bp ds-DNA PLN 26-MAY-1991
DEFINITION Rice 4-CL gene for 4-coumarate-CoA ligase (EC 6.2.1.12)
ACCESSION X52623
KEYWORDS 4-coumarate-CoA ligase; ligase.
SOURCE Oryza sativa DNA.
ORGANISM Oryza sativa
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
Commelinidae; Cyperales; Poaceae.
REFERENCE 1 (bases 1 to 5225)
AUTHORS Dube,S.K.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 5225)
AUTHORS Zhao,Y., Kung,S.D. and Dube,S.K.
TITLE Nucleotide sequence of rice 4-coumarate:CoA ligase gene, 4-CL.1
JOURNAL Nucleic Acids Res. 18, 6144-6144 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17814; 4CL$ORYSA.
*source: strain=Japonica; library=EMBL-3; clone=4-CL;
Data kindly reviewed (10-AUG-1990) by Dube S.
From EMBL entry OS4CL; dated 26-OCT-1990.
FEATURES Location/Qualifiers
mRNA <1594..1593
/note="exon 1"
CDS 541..1593
/note="4-coumarate-CoA ligase (AA 1-351)"
/codon_start=541
intron 1594..2531
/note="intron I"
mRNA 2532..2730
/note="exon 2"
CDS 2532..2730
/note="4-coumarate-CoA ligase (AA 352-417) (2730 is 1st
base in codon)"
/codon_start=2532
intron 2731..4211
/note="intron II"
mRNA 4212..4425
/note="exon 3"
CDS 4212..4425
/note="4-coumarate-CoA ligase (AA 418-489) (4212 is 2nd
base in codon) (4425 is 2nd base in codon)"
/codon_start=4212
intron 4426..4574
/note="intron III"
mRNA 4575..4668
/note="exon 4"
CDS 4575..4668
/note="4-coumarate-CoA ligase (AA 490-520) (4575 is 3rd
base in codon)"
/codon_start=4575
intron 4669..4785
/note="intron IV"
mRNA 4786..>4785
/note="exon 5"
CDS 4786..4914
/note="4-coumarate-CoA ligase (AA 521-563)"
/codon_start=4786
misc_feature 5022..5027
/note="polyA signal"
BASE COUNT 1448 a 1206 c 1126 g 1445 t
ORIGIN
1 ccagaacttc atgatgaaaa gtttaaaact gttcttttcc aagctataat caagattttc
61 gtaaatatag gttatctttt ctaagttcac atgtcacacg cgtttgacag tgtcgtgtta
121 ttcttcacca aaatctacca tacaaaatct ctgtacatgt aaaagtaaaa catagtatga
181 tgtgcagagc ataaataata tcacatcact ctttttcaac ttttttttaa aaaaaatcca
241 gatcaattaa ttcatctaag atagaagtca aacacatacg gagataatct aggaccatat
301 tgcatatact taactaacaa tttgtacatg ttacatctac taatctccat tccatgtttg
361 acttccaatc gaacagctaa gcttattatg ccatattatt tcatttagcc ccttggaatt
421 cctccatttt acaaatacat ccctcaccaa tctcatttct atacatatca tcatcgtttc
481 ctccaaactc agaagcccaa aacacacgaa gcaaagctgc ctcgtctgtg tcactgacag
541 atggggtcca tggagcagca gcagccggag tcggcggcgc cggcgacgga ggcgtcgccg
601 gagatcatct tccggtcgaa gctgcaagac atcgccatca ccaacaccct cccgctccac
661 cgctactgct tcgagcggct cccggaggtg gcggcgcgcc catgcctcat cgacggagcc
721 accggcggcg tgctcaccta cgccgacgtc gaccgcctgt cccgccgcct cgccgccgcg
781 ctccgccgcg cgccgctggg gctccggcgg ggcggcgtcg tgatgagcct gctgcggaac
841 tcgccggagt tcgtgctgtc cttcttcgcc gcgtcgcgcg tcggcgccgc ggtgaccacg
901 gcgaacccaa tgtccacccc gcacgagatc gagagccagc tcgccgccgc cggcgccacc
961 gtcgtcatca ccgagtccat ggccgccgac aagctcccct cccactccca cggcgccctc
1021 accgtcgtcc tcatcgacga gcgccgcgac ggctgcctcc acttctggga cgacctcatg
1081 tcggaggacg aagcgtcgcc gctcgccggc gacgaggacg acgagaaggt gttcgacccg
1141 gacgacgtgg tggcgctgcc gtactcgtcg gggacgacgg gtctccccaa gggggtgatg
1201 ctaacgcacc ggagcctgag cacgagcgtg gcgcagcagg tcgacggcga gaacccaaac
1261 atcgggctcc acgccggcga cgtgatcctg tgcgcgctgc ccatgttcca catctactcg
1321 ctcaacacca tcatgatgtg cggcctccgc gtcggcgccg ccatcgtcgt catgcgccgc
1381 ttcgacctcg ccgccatgat ggacctcgtc gagcgccacc gcgtcaccat cgcgccgctc
1441 gtgccgccca tcgtcgtggc cgtggccaag agcgaggcgg cggcggcgcg agacctctcg
1501 tcggtgcgga tggtgctctc cggcgcggcg cccatgggca aggacatcga ggacgcgttc
1561 atggccaagc tccccggcgc cgtgctcgga caggtacaat acaagaaaaa tattttcctt
1621 tttttggttg aattggttgt cattttttgt gcgccgttgg atcatccgat gggcggctcg
1681 gattccatgt tgactttggc gtctgacggt caacggtaat ctgagccctc catttggctg
1741 gtgtacagat ccaatggcgg gaaatcttaa cgtgtgaagg actcaagaag cccttctttt
1801 cacgttttta tttaggtttt gatacaatat atacgcaata ggtgttgttt tttaaaaaaa
1861 ataaaacaaa atggacatta cattattggc tgtgtttaga tccaaaattt agatccaaac
1921 ttcaatcctt ttccatcaca tcaacctgtc atacacacac aacttttcag tcacatcatc
1981 tccaatttca accaaaatcc aaactttgga tccaactaaa cacaaccatt gttatatata
2041 tataaactta taaaaaggac atgaaaacaa ttaatgaaac taaggacaga tttcatttca
2101 ctgacatgct tagaaagtat agttgccaat atatgttatc tttttaatgc gtgtgtgtgt
2161 gtgtgtaaag tggagctcca ctcccacaca caaacatgat ggcatgacta ttaataagct
2221 aagttggctc aagtcaacat ctgaggccaa taatatcttc tagctagttt ttcttttaaa
2281 aaaaatccta gctaactgat atgatataaa ttagcttgcc atttactcaa aagaaaagaa
2341 gtatcttatt ccccaatgca ataacatggt agtattattt taaatcccac atactagcac
2401 taaattcacc tttggtttgt tctatatgac atagatatca aattagtcct acaaaaatgt
2461 tatattctaa gcatgtccag ctaattaagc aaatgtggtg atgatctttg ctgcccacta
2521 tgtgcattca ggggtatgga atgactgagg cagggccagt gctgtcaatg tgcctagcat
2581 tcgccaagga gcccttcaag gtgaagtccg gtgcgtgcgg gacggtggtg cgcaacgccg
2641 agctgaagat catcgacccc gacaccggga agtcgcttgg ccggaacctg cgcggcgaga
2701 tctgcatcag ggggcaacag atcatgaaag gtacagaaga tccctgaaca tcttggcatg
2761 ttcaactgtt gacttctcag ttatgttttg cccctgcaat agagaatgta cagaagatcc
2821 ctgaacatct tggcatgttc aactgttgac ttctcagtta tgttttgccc ctgcaataga
2881 gaatgtacta cactaccact ggcccagatc tctagatcaa atctgacttg gaaaacatat
2941 gataatcacc agtggataac cagctacaca aaaaattata ctacagcatt gactggattt
3001 ctttttgact attattaaac ctgaattatg acaagtagtt gtctggagat gatgtgcaat
3061 aaaacaaggt caaaaatatg gatggccctc tctttctaat ggaagtaaaa gaaagtgttg
3121 ctttggagaa atgataatac atgttgtagt gttgcttttt tatagggtca tgtgcaagaa
3181 gtccacagta ggtaggatat gtaatcctaa ttgagtagat gctccagcta gcgaattatg
3241 ttgatgcaac tttgatgtca agatctcaac tagcgtgcac tggcatgtca catatccgat
3301 gccgttggtt caaatcattc ctagcaattc catcgcgcta ttaatgttcg cctcccatct
3361 ttcgccctat aactttataa gccatagctg aaattaaaat tttgaaaatt aattttggag
3421 ttaattttgt tttttatgta gtttctttcc tagtattggt ttttcaattg ctaatggcat
3481 aaatataaaa gtttcactca taaattaaaa cgggttatca gctctaggcc aaacggtagc
3541 gctgttgcca aacgatatca atggaattat gcgagttctt aaggccgtat tcacaattat
3601 aggtcttttt tttttaggac acaattatag gtctaacaga gtaaggatgt tgctgttcgt
3661 cattgtatag agcggaaggt ctgaaaggct gaaacaatca aacaattgtt gtttaatttc
3721 cacactacta atatatgcgt ccaagacctg ggtaaagttt agaaaatgag agatgtcaaa
3781 tttgtcactt cagttgcatc actgcaaatg tactataaat atatatcagt agctaatagt
3841 aatttttcaa gttgcagttt aaatgcaaat atcccagccc aagtgactcg cattcaaaaa
3901 agaaacagtg acagtgacaa cgaaaggtcc ttggaataat atcagaactg aaattttata
3961 gcctcatcgt ctcgcttatc tcataaggga catggctcat tagtgattta aaactagttt
4021 gtgggtatat gtgttcttag tgactaaaac aaaatgctaa aaaataaatt acgatgaaaa
4081 aaaccataaa attaaatttt aaattttaaa tttttcgctt atgcttatga gtgatcacct
4141 gtatctaacc acctattcgt tgtactaatt tccatgaaaa ttaacatgct aaaaaaaccg
4201 ttatttttca ggttacctga acaacccaga ggccacgaag aacaccattg atgcagaagg
4261 gtggctgcac accggcgaca tcggctacgt cgacgacgac gacgagatct tcatcgtgga
4321 taggctcaag gagatcatca aatacagggg cttccaggta gcccctgcag agctggaggc
4381 ccttctcaac acccatccaa gcattgctga tgctgctgtt gttgggtatg tcttaactag
4441 ctcctgtctg aaaatgtagt aaggtttaat tgcttgatag ataatgttgc agagttttga
4501 catggatttg aatgaatcag tcttgaaatg gcgttttcat gatatgattg gccacttgca
4561 ggaaacaaat tgagcctgaa attcggagaa ataccagtcg ccttcgtggc gaaaaccgaa
4621 gggagtgagc tgagtgaaga tgatgtcaaa caattcgtag caaaggaggt aaattccttt
4681 accatggtta ttcataggac aaaaagagaa cactttacca tggctgagta ttgttttttt
4741 ttttactttt ccttcattaa attattcatt tatatgtggt tttaggtgat ctactacaag
4801 aagatccggg aggtcttctt cgttgacaaa atccccaagg cgccgtcagg gaagatcctg
4861 agaaaggaac tgaggaagca gctgcaacat cttcaacaag aagctttgac gaattaggcc
4921 agctttgtac aggtttagat gtttagtgac ataaaagtgt caccatcaca ttatcatttc
4981 aatgcacttc catgttttgg ttttcttagt agtaactgca agataaattt gtggtgaaac
5041 tacacttcaa tggacttgtg ccaagtcaaa cgacatcgga tcgaaatgga tggtgggggt
5101 aactagtgta ttcaaactct tgactacttc ctctcttcca gtatgcttca gagttgcttg
5161 gctgttttcc tgatatctct tgttatgcta aattgatgac tggtggcatt gtaccattgg
5221 ctgca
//