GenBank-Updates@genbank.bio.net (05/26/91)
LOCUS MZEPEP 6781 bp ds-DNA PLN 26-MAY-1991
DEFINITION Z.mays gene for phosphoenolpyruvate carboxylase (EC 4.1.1.31)
ACCESSION X15642
KEYWORDS carboxylase; phosphoenolpyruvate carboxylase.
SOURCE Zea mays DNA.
ORGANISM Zea mays
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
Commelinidae; Cyperales; Poaceae.
REFERENCE 1 (bases 1 to 6781)
AUTHORS Matsuoka,M. and Minami,E.
TITLE Complete structure of the gene for phosphoenolpyruvate carboxylase
from maize
JOURNAL Eur. J. Biochem. 181, 593-598 (1989)
STANDARD full automatic
REFERENCE 2 (bases 1 to 6781)
AUTHORS Matsuoka,M. and Minami,E.
JOURNAL Unpublished (1990)
STANDARD full automatic
COMMENT *source: strain=cv. Golden Cross Bantam;
Data kindly reviewed (20-AUG-1990) by Matsuoka M.
From EMBL entry ZMPEP; dated 02-OCT-1990.
FEATURES Location/Qualifiers
precursor_RNA 1213..>1212
/note="primary transcript"
mRNA 1213..1476
/note="exon 1"
CDS 1297..1476
/note="P-pyruvate carboxylase (AA 1 - 60)"
/codon_start=1297
intron 1477..1586
/note="intron I"
mRNA 1587..1981
/note="exon 2"
CDS 1587..1981
/note="P-pyruvate carboxylase (AA 61 - 192) (1981 is 2nd
base in codon)"
/codon_start=1587
intron 1982..2091
/note="intron II"
mRNA 2092..2176
/note="exon 3"
CDS 2092..2176
/note="P-pyruvate carboxylase (AA 193 - 220) (2092 is 3rd
base in codon)"
/codon_start=2092
intron 2177..3073
/note="intron III"
mRNA 3074..3296
/note="exon 4"
CDS 3074..3296
/note="P-pyruvate carboxylase (AA 221 - 294) (3296 is 1st
base in codon)"
/codon_start=3074
intron 3297..3745
/note="intron IV"
mRNA 3746..3849
/note="exon 5"
CDS 3746..3849
/note="P-pyruvate carboxylase (AA 295 - 329) (3746 is 2nd
base in codon)"
/codon_start=3746
intron 3850..3974
/note="intron V"
mRNA 3975..4065
/note="exon 6"
CDS 3975..4065
/note="P-pyruvate carboxylase (AA 330 - 359) (4065 is 1st
base in codon)"
/codon_start=3975
intron 4066..4202
/note="intron VI"
mRNA 4203..4357
/note="exon 7"
CDS 4203..4357
/note="P-pyruvate carboxylase (AA 360 - 411) (4203 is 2nd
base in codon)"
/codon_start=4203
intron 4358..4453
/note="intron VII"
mRNA 4454..5452
/note="exon 8"
CDS 4454..5452
/note="P-pyruvate carboxylase (AA 412 - 744)"
/codon_start=4454
intron 5453..5549
/note="intron VIII"
mRNA 5550..5936
/note="exon 9"
CDS 5550..5936
/note="P-pyruvate carboxylase (AA 745 - 873)"
/codon_start=5550
intron 5937..6024
/note="intron IX"
mRNA 6025..>6024
/note="exon 10"
CDS 6025..6315
/note="P-pyruvate carboxylase (AA 874 - 970)"
/codon_start=6025
polyA_site 6588..6588
/note="polyA site"
BASE COUNT 1687 a 1851 c 1551 g 1692 t
ORIGIN
1 tctagagatg taatggtgtt aggacacgtg gttagctact aatataaatg taaggtcaaa
61 attcgatggt ttattttcta ttttcactta cctagcatta tctcatttct aattgtgtga
121 taacaaatgc attagaccat aattctgtaa atacgtacat ttaagcacac agtctatatt
181 ttaaaattct tctttttgtg tggatatccc aacccaaatc cacctctctc ctcaatccgt
241 gtatcttcac cgctgccaag tgccaacaac acatcgcatc gtgcaaatct ttgttggttt
301 gtgcacggtc ggcgccaatg gaggagacac ctgtacggtg cccttggtag aacaacatcc
361 ttatccctat atgtatggtc ccttcgtaga tggcaccctt atccctacaa tagccatgta
421 ttgcatacca agaattaaat atactttttc ttgaaccaca ataatttatt atagcggcac
481 ttcttgttct ggttgaacac ttatttggaa caataaaatc ccgagttcct aaccacaggt
541 tcactttttt tccttatcct cctaggaaac taaattttaa attcataaat ttaattgaaa
601 tgttaatgaa aacaaaaaaa ttatctacaa agacgactct tagccacagc cgcctcactg
661 caccctcaac cacatcctgc aaacagacac cctcgccaca tccctccaga ttcttccctc
721 cgatgcagcc tacttgctaa cagacgccct ctccacatcc tgcaaagcat tcctccaaat
781 tcttgcgatc ccccgaatcc agcattaact gctaagggac gccctctcca catcctgcta
841 cccaattagc caacggaata acacaagaag gcaggtgagc agtgacaaag cacgtcaaca
901 gcaccgagcc aagccaaaag gagcaaggag gagcaagccc aagccgcagc cgcagctctc
961 caggtcccct tgcgattgcc gccagcagta gcagacaccc ctctccacat cccctccggc
1021 cgctaacagc agcaagccaa gccaaaaaga agcctcagcc acagccggtt ccgttgcggt
1081 taccgccgat cacatgccca aggccgcgcc tttccaaacg ccgagggccg cccgttcccg
1141 tgcacagcca cacacacacc cgcccgccaa cgactcccca tccctatttg aacccacccg
1201 cgcactgcat tgatcaccaa tcgcatcgca gcagcacgag cagcacgccg tgccgctcca
1261 accgtctcgc ttccctgctt agcttcccgc cgcgccatgg cgtcgaccaa ggcacccggc
1321 cctggcgaga agcaccactc catcgacgcg cagctccgtc agctggtccc aggcaaggtc
1381 tccgaggacg acaagctcat cgagtacgat gcgctgctcg tcgaccgctt cctcaacatc
1441 ctccaggacc tccacgggcc cagccttcgc gaatttgtaa ctaaccaccg ccgccgccca
1501 tttcttcttc gaccggttgc cgcctgcgcg cggcactgct cgtgtcgtgt gcttagtctt
1561 agtgcttact actgtaatgc atgcaggtcc aggagtgcta cgaggtgtcg gccgactatg
1621 agggcaaagg agacacgacg aagctgggcg agctcggcgc caagctcacg gggctggccc
1681 ccgccgacgc catcctcgtg gcgagctcca tcctgcacat gctcaacctc gccaacctgg
1741 ccgaggaggt gcagatcgcg caccgccgcc gcaacagcaa gctcaagaaa ggtgggttcg
1801 ccgacgaggg ctccgccacc accgagtccg acatcgagga gacgctcaag cgcctcgtgt
1861 ccgaggtcgg caagtccccc gaggaggtgt tcgaggcgct caagaaccag accgtcgacc
1921 tcgtcttcac cgcgcatccc acgcagtccg cccgccgctc gctcctgcag aaaaacgcca
1981 ggtatatatt tctcaatggc ttgatcgata tgctactcac gttatatacc cttaagtctt
2041 aaccattatt attatttttg ataaataaaa atgtcggtct tgtcgctgca ggatccggaa
2101 ttgtctgacc cagctgaatg ccaaggacat cactgacgac gacaagcagg agctcgatga
2161 ggctctgcag agagaggtac gtacatatta catttcacac cagggaatgc aagaacttta
2221 tcaagagaca ttcattcttt gatagagata gaatagaaca catgcacagt acacgtggac
2281 tcatgagctt gcaagacatc gagcacgaca cgtgtaagtt agtgcgccag agaaatcttc
2341 aatttatatg tcaagtcagg tcaggttctc ccattaaaac acatataaat aaatattcat
2401 tattatcaag ctaaggtaat aaacaaccaa acttttccac tatttaaact gtctttgcaa
2461 actccaaagt agaaactaac ctaatcagga aagaactaga ctgcacattt atgttttaac
2521 aatgcaatga gagaactgct acatgtataa cagaattatt tatatgaggc cgacttgact
2581 taagattcaa tgttgaagac cacttgatga aaactacact gaattattta tatgctattc
2641 tccagctgtg ctcaaagcat tttcctttta cttaaaaaag atcattttgt acaaagatct
2701 cttactcata tagagccatt tgagtagaac ttcggtacca cagatgcatt aatggtttag
2761 ttgtaatcaa gttgttgtac tcatcattat attttcctaa caagtagggc atccagtttc
2821 tccttgatga ggaatcaaac ctagatagcc ttaactccac accctcaatt agctaggcta
2881 tgctcaagtt cctagtgtta caaatttcag acgagtcata atgtcatcac tgagcactcg
2941 gtaaagagcg tctctctcat ggtgcatata tatgatgcag accacctgag aagtttactg
3001 cttcaagcca ccaaagtggt atttttgttg tttgggttgt ttagttctaa ttccttttct
3061 tgggtgttca cagatccaag cagccttcag aaccgatgaa atcaggaggg cacaacccac
3121 cccccaggac gaaatgcgct atgggatgag ctacatccat gagactgtat ggaagggcgt
3181 gcctaagttc ttgcgccgtg tggatacagc cctgaagaat atcggcatca atgagcgcct
3241 tccctacaat gtttctctca ttcggttctc ttcttggatg ggtggtgacc gcgatggtac
3301 atttctgcct acccttttca ataaagtggc aggagctctc tgtctttcag cttgagagaa
3361 accttcctgc tttactctga ctgcaataga tgttcagaaa aactagtcta tcatttcgag
3421 ctctcaggag ctagaatttt aaaattgaaa ttatttagta cacctcacta ataaaaattt
3481 atcatccata catgctagca caacatataa gcataattta atcaaatctt tatattgcaa
3541 cctggaacct aacttgttga attttttata tcacagaatt atacgtgtag tattatttta
3601 tatatcaaag agtgcttata ttatatcagt acttgtcctg tcaatattca aggctaacgt
3661 ttttcttttc tcgccagaaa attatatata cagaattata tgttttttct aagcctgtat
3721 atctttgcaa tctatcgcta tataggaaat ccaagagtta ccccggaggt gacaagagat
3781 gtatgcttgc tggccagaat gatggctgca aacttgtaca tcgatcagat tgaagagctg
3841 atgtttgagg tactgtacat ccatactgca gatttgtttg attgaatgct ctatgatttt
3901 ttgcttgccc tgttttttgc tgtctccggt ccataccaga actctcatgc atgcatcgtc
3961 tgatatatct gtagctctct atgtggcgct gcaacgatga gcttcgtgtt cgtgccgaag
4021 agctccacag ttcgtctggt tccaaagtta ccaagtatta cataggtaac cacaaacaga
4081 agcatttatg tttgcttaat ttttgcctgc cgtacaggct tttgcaaaag tctccactag
4141 tgttttcaaa ttaatttgag ggctcttttg gcatcttttc tgaagtgtat ttgctggcgc
4201 agaattctgg aagcaaattc ctccaaacga gccctaccgg gtgatactag gccatgtaag
4261 ggacaagctg tacaacacac gcgagcgtgc tcgccatctg ctggcttctg gagtttctga
4321 aatttcagcg gaatcgtcat ttaccagtat cgaagaggta aatatcgtca tgtatatatt
4381 atatatattc atagtatgac atcagcactg caactaacaa aaaaaaaatc actactgtcg
4441 tgcatgcatg cagttccttg agccacttga gctgtgctac aaatcactgt gtgactgcgg
4501 cgacaaggcc atcgcggacg ggagcctcct ggacctcctg cgccaggttt tcacgttcgg
4561 gctctccctg gtgaagctgg acatccggca ggagtcggag cggcacaccg acgtgatcga
4621 cgccatcacc acgcacctcg gcatcgggtc gtaccgcgag tggtccgagg acaagcggca
4681 ggagtggctg ctgtcggagc tgcgaggcaa gcgcccgctg ctgcccccgg accttcccca
4741 gaccgaggag atcgccgacg tcatcggcgc gttccacgtc ctcgcggagc tcccgcccga
4801 cagcttcggc ccctacatca tctccatggc gacggccccc tcggacgtgc tcgccgtgga
4861 gctcctgcag cgcgagtgcg gcgtgcggcc agccgtgccc gtggtgccgc tgttcgaaag
4921 gctggccagc ctgcagtcgg cgcccgcgtc cgtggagcgc ctcttctcgg tggactggta
4981 catggaccgg atcaagggca agcagcaggt catggtcggc tactccgact ccggcaagga
5041 cgccggccgc ctgtccgcgg cgtggcagct gtacagggcg caggaggaga tggcgcaggt
5101 ggccaagcgc tacggcgtca agctcacctt gttccacggc cgcggaggca ccgtgggcag
5161 gggtggcggg cccacgcacc ttgccatcct gtcccagccg ccggacacca tcaacgggtc
5221 catccgtgtg acggtgcagg gcgaggtcat cgagttctgc ttcggggagg agcacctgtg
5281 cttccagact ctgcagcgct tcacggccgc cacgctggag cacggcatgc acccgccggt
5341 ctctcccaag cccgagtggc gcaagctcat ggacgagatg gcggtcgtgg ccacggagga
5401 gtaccgctcc gtcgtcgtca aggaggcgcg cttcgtcgag tacttcagat cggtatgctg
5461 ccattgccca ttgctttgtg acgatcgaat tcatccatgt cgatcgttct tttcattcat
5521 tcgagcgttt gtgcgtcact cactatcagg ctacaccgga gaccgagtac gggaggatga
5581 acatcggcag ccggccagcc aagaggaggc ccggcggcgg catcacgacc ctgcgcgcca
5641 tcccctggat cttctcgtgg acccagacca ggttccacct ccccgtgtgg ctgggagtcg
5701 gcgccgcatt caagttcgcc atcgacaagg acgtcaggaa cttccaggtc ctcaaagaga
5761 tgtacaacga gtggccattc ttcagggtca ccctggacct gctggagatg gttttcgcca
5821 agggagaccc cggcattgcc ggcttgtatg acgagctgct tgtggcagaa gaactcaagc
5881 cctttgggaa gcagctcagg gacaaatacg tggagacaca gcagcttctc ctccaggtac
5941 caaaaccagc actgcactgt acgatatgaa taaaagtctg ttgtctggct cctgatcgat
6001 gactgactac tccattttgt gcagatcgct gggcacaagg atattcttga aggcgatcca
6061 ttcctgaagc agggactggt gctgcgcaac ccctacatca ccaccctgaa cgtgttccag
6121 gcctacacgc tgaagcggat aagggacccc aacttcaagg tgacgcccca gccgccgctg
6181 tccaaggagt tcgccgacga gaacaagccc gccggactgg tcaagctgaa cccggcgagc
6241 gagtacccgc ccggcctgga agacacgctc atcctcacca tgaagggcat cgccgccggc
6301 atgcagaaca ctggctaggc ggcttctctt cactcacctg cagagtgcac cgcaataatc
6361 agcttccgga tggtggcgtt ttgtcagttt tggatggaaa tgccgaactg gcagcgtctg
6421 ttttccctat gcatatgtaa tttcctgcct ctttatattc actcttgttg tcaagtccaa
6481 gtggaaaatc ttggcatatt atacatattg taataataaa catcgtacaa tctgcatgct
6541 gttttgtaat aattaattaa tatcccagcc cattggatgg acttgtttac catggtgtta
6601 cttcagccac cctctcttag ttgtgctaaa cattttctga ttggtatttt ttttattaga
6661 gtaacctagt gcatttactt aagagagatg atatctagtg gcactagtga ttagtttgca
6721 agattgagaa cttgttactc gctcctagag gttaacacta gcaagtgatt ggagcttagg
6781 g
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