GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS PASTOXA 4308 bp ds-DNA BCT 27-MAY-1991
DEFINITION Pasteurella multocida toxA gene for dermonecrotic toxin
ACCESSION X52478
KEYWORDS dermonecrotic toxin; toxA gene.
SOURCE Pasteurella multocida DNA.
ORGANISM Pasteurella multocida
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Pasteurellaceae.
REFERENCE 1 (bases 1 to 4308)
AUTHORS Smits,M.A.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 4308)
AUTHORS Buys,W.E., Smith,H.E., Kamps,A.M., Kamp,E.M. and Smits,M.A.
TITLE Sequence of the dermonecrotic toxin of pasteurella multocida ssp.
multocida.
JOURNAL Nucleic Acids Res. 18, 2815-2816 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17452; TOXA$PASMU.
*source: strain=ssp. multocida, CVI47459; *source: library=lambda
GEM11; clone=5 and 8;
From EMBL entry PMTOXA; dated 26-JUL-1990.
FEATURES Location/Qualifiers
promoter 48..54
/note="pot. -35 region"
promoter 68..74
/note="pot. -10 region"
RBS 185..194
/note="ribosome binding site"
CDS 199..4053
/note="dermonecrotic toxin (AA 1-1285)"
/codon_start=199
terminator 4072..4100
/note="pot. stem loop structure"
misc_feature 4103..4107
/note="pot. transcription termination site"
BASE COUNT 1484 a 705 c 783 g 1336 t
ORIGIN
1 ttagacgata tcgataatat cataaataat atttaaaaat acgccccttg acctagaggg
61 gcttttttat tacatcaaaa aaataaaccc caaacactgc gaatgtttgg ggttttattt
121 ataaccaaaa tacattaata tgtttattaa gtaagcatta tcttacttta ggaataaact
181 aacatagagg ttatggatat gaaaacaaaa cattttttta actcagattt tactgtaaaa
241 ggaaaaagtg ccgatgaaat ttttagaaga ttgtgtactg atcatcctga caagcaatta
301 aacaatgtaa aatggaaaga agtttttatt aatcgttttg gtcagatgat gctagatact
361 cctaatccga gaaagattgt agaaaaaatt attaatgaag ggcttgaaaa acaaggcctg
421 aaaaatatag atcctgaaac tacatatttc aacatttttt catcttctga cagctccgat
481 gggaacgttt ttcattataa ctctttatca gaatcctatc gagttactga tgcctgccta
541 atgaatattt ttgtggagcg ttattttgat gattgggact tgctaaatag cttagccagt
601 aatggaatat attcagtagg aaaagaagga gcttattatc ctgatcatga ttatggtcca
661 gaatataacc ctgtttgggg accaaacgaa caaatttacc attctagagt gattgcagat
721 atcctttatg ctcgctccgt atgggatgaa tttaaaaaat acttcatgga gtattggcaa
781 aaatatgctc agctttatac cgaaatgtta tctgatacat ttcttgcaat ggctattcag
841 caatatacac gacaaacgct tactgatgaa ggctttctta tggtttgtaa cacatattat
901 ggcaataagg aagaagttca aataactcta ctagatatct atggataccc ttccactgat
961 ataatttgta tagagcaaaa agggcttcct actcctaaag tgatacttta cattcctgga
1021 ggaacacaac catttgttga atttcttaat acagatgatc tgaaacaatg gattgcatgg
1081 catttaaaag ataacaaaca tatggtcgca ttccgcaaac atttctcgct aaaacaacgt
1141 caggaaggag aaacgtttac aggtatagat aaagcacttc aatatattgc agaagagtcc
1201 cctgaatggc ctgccaataa atacatcctt tataatccga cacatttaga aacagaaaat
1261 ttatttaaca tcatgatgaa gcgaacagaa cagcggatgc ttgaagatag tgatgtacag
1321 attagatcaa attcagaagc tacccgtgac tatgctcttt cattactcga aacctttatt
1381 tcacagttat ctgcaataga tatgttagta ccagcagtag gtatcccaat taattttgcc
1441 ctatcagcta cagcattagg acttagctcg gatattgtag ttaatggaga ttcatatgaa
1501 aagagaaaat atggaattgg gtccttagtg caatctgcat tattcacagg aattaatctt
1561 attccagtta tttcggaaac cgcagaaatt ttatcttctt tctctagaac agaagaagat
1621 attccagctt ttttcactga agaacaagct ttagctcaac gctttgaaat agtagaagaa
1681 gaattacatt ctatctcacc tgatgatcct cctcgagaaa ttactgacga aaatttacat
1741 aaaattcgtc tggtacgtct taacaatgaa aatcaacctt tagttgtgtt acgaagatta
1801 ggaggaaata aatttatcag aatcgagcct ataacattcc aggaaataaa aggttcttta
1861 gtaagtgaag ttataaatcc agtgactaat aaaacgtact acgtaagcaa tgctaaacta
1921 ttagggggct ctccttatag tcctttccgt attggattag aaggtgtttg gacaccagag
1981 gtattaaaag caagagcttc cgttattgga aagcctattg gagaatcata taaaagaata
2041 ttagccaaac tacaaagaat acataacagt aatatcttag atgagcgaca aggtttaatg
2101 catgaactca tggagcttat tgatctttat gaagaatcgc aaccttcttc agagcgtttg
2161 aatgcttttc gtgaactgcg tactcaatta gaaaaagcgc tttatcttcc tgaaatggaa
2221 gcattaaaaa aacaaatact acagattcct aacaaaggtt ctggtgccgc tcgattttta
2281 cttcgtacag ccatgaatga aatggctgga aaaaccagtg aaagcacggc tgatttaata
2341 cgctttgcct tgcaagatac agtaatttca gcgccttttc gcggatatgc tggtgcgatt
2401 ccagaggcaa tagactttcc tgtaaaatat gtaatagaag acatatctgt atttgataaa
2461 atacagacaa attactggga acttcctgct tatgaaagct ggaacgaagg aagtaatagc
2521 gcattactgc ctggtttgtt acgtgaatcg caaagcaagg ggatgttaag taagtgtcgt
2581 atcatagaaa atagccttta tattggacat agctatgaag aaatgtttta cagcatttct
2641 ccatattcaa accaggttgg agggccttat gaattatatc ctttcacttt tttcagtatg
2701 cttcaagaag tacaaggtga tttaggattt gagcaggcct ttgccacacg taactatttc
2761 aatactcttg tttctgatcg actatcctta atggaaaata cgatgttact tacagaaagt
2821 tttgattata caccttggga tgctatttat ggagatatta attatgatga acaatttgct
2881 gcaatgtcta ttaatgaacg catagaaaaa tgtatgaata cctatagagg tgtggcattc
2941 caaaactctt caaaaagtat tgactttttc ctaaataatc taaccacatt cattgataat
3001 ggactaaccg aaattgctat atctgattta ccgtatgata ttgtgcaaca agaaatctct
3061 caattcttac aaggaagtaa tgaatggaaa acacttgatg ccatgttatt taacttagat
3121 aaaggagata ttaatggtgc tttcagaaag cttctgcaat cagcaaaaga taataatata
3181 aaatttagag ctatagggca ttcagataat tctgttccgc catttaataa cccttataag
3241 tctttatatt ataaaggaaa tataatagct gaagcaattg aaaaactaga tcgagaaggt
3301 caaaaatttg ttgtatttgc tgatagttct ctgctcaaca gcacgcctgg gacaggtcgt
3361 cctatgccag gactagttca atatttaaaa ataccagcaa ctgtagtaga tagcgatggt
3421 gcatggcaat ttcttccaga tgtagcttca agcagagttc ctattgaagt tacagagtta
3481 gaaaattggc aagtcttaac tcctccacaa ggtaagattc ttggattaaa gcaatttaag
3541 ttaacggcag gttttccaac agaacaaagt cgcttacctc ttttagagaa ttcggtttct
3601 gaagatttaa gggaagaatt aatgcaaaag attgatgcaa taaaaaatga tgtgaaaatg
3661 aatagtttag tgtgtatgga agctggctct tgtgattcag taagccctaa ggtagctgcc
3721 cgtcttaaag atatggggtt agaagctggg atgggtgctt ctattacctg gtggagacgt
3781 gaaggcggga tggaattttc acatcagatg catactactg cttcctttaa atttgctggt
3841 aaagagtttg ccgtggatgc ttcacattta caatttgtac acgaccaatt agatacaact
3901 atcctgatac tacctgtaga tgattgggct ttagaaatag ctcaaagaaa tcgggctatt
3961 aatccttttg tggaatatgt tagtaaaaca ggaaacatgt tagcactctt catgcctcct
4021 cttttcacaa agcctcgctt aacaagagca ctataactaa ttaaaaactg tattaaagcc
4081 ttatattata aggctttaat tttctttcaa gaattattaa gtagaagaat caaaatcaat
4141 gagatagata aaatcaaatg ttattaccaa tacaactttc ttaagtatac tttttgaatt
4201 ttttgcgtta ataaatttat aataccctta actcaataaa agaagttatt gagaagttta
4261 aatcttgtga gcaagatgaa gatataattt cagcaatcga tcgagctc
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