GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS PSEALKST 2118 bp ds-DNA BCT 27-MAY-1991
DEFINITION P.oleovorans alkS (partial) and alkT genes encoding AlkS regulatory
protein (partial) and rubredoxin reductase
ACCESSION X52935
KEYWORDS NAD dependent flavoprotein oxidoreductase; alkS gene; alkT gene;
positive regulatory protein; regulatory protein;
rubredoxin reductase.
SOURCE Pseudomonas oleovorans DNA.
ORGANISM Pseudomonas oleovorans
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 1 to 2118)
AUTHORS Terpstra,P.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2118)
AUTHORS Eggink,G., Engel,H., Vriend,G., Terpstra,P. and Witholt,B.
TITLE Rubredoxin reductase of Pseudomonas oleovorans. Structural
relationship to other flavoprotein oxidoreductases based on one NAD
and two FAD fingerprints.
JOURNAL J. Mol. Biol. 212, 135-142 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17051; ALKS$PSEOL. SWISS-PROT; P17052; ALKT$PSEOL.
*source: strain=TF4-1L (+ OCT Plasmid);
From EMBL entry POALKST; dated 19-JUN-1990.
FEATURES Location/Qualifiers
CDS <1..672
/note="AlkS regulatory protein (224 AA)"
/codon_start=1
RBS 712..717
/note="ribosome binding site"
CDS 725..1879
/note="rubredoxin reductase (AA 1-385)"
/codon_start=725
BASE COUNT 594 a 424 c 562 g 538 t
ORIGIN
1 atctatcagc gcttagtctg tcaaggcata acgggcataa ataatttaaa aactcttgaa
61 gatcataaga ttttctccgg acagcactca gcccccctaa aagcacgcct gctgcttgtt
121 caatcactag tgctttcccg agatcggaac tttcatagtg ccgcgcacag agcgttattg
181 gctattcagc aagcccgtaa aattaacgcg ggccagctgg aagtccgtgg attattgtgt
241 ttggccggag cgcaggcagg tgccggtgat ttaaaaaagg ctcagcttaa cattgtttat
301 gcagtggaga tagcaaaaca gcttcaatgc tttcaaacag ttcttgatga agtatgttta
361 attgagcgaa taataccggc ttcatgtgaa gccttcacag cagttaattt agatcaagcg
421 attggggctt ttagtcttcc gcgaatagtt gagattggaa agtccgcaga gaataaagct
481 gacgctttat tgacacggaa gcagattgct gtcttgaggc ttgtaaaaga ggggtgctca
541 aacaaacaaa tagcaacaaa tatgcatgtc accgaagatg ctataaagtg gcatatgagg
601 aaaatatttg ccaccttgaa tgtagtgaat cgcacgcaag caacaattga agctgagcgt
661 caaggaatta tctaaaataa tcggcattaa gtgatatagt gaaaagtata ccggagagag
721 aattatggca atcgttgttg ttggcgctgg tacagctgga gtaaatgctg cgttctggct
781 tcgtcaatat ggttataaag gggaaattag gatttttagc agggagtctg tggcgcctta
841 tcagcggcct cctctatcca aggcttttct gacaagtgag attgcagaat ccgcagtgcc
901 attaaagcca gaaggttttt atacgaataa caatattacc atttcgttaa atacaccgat
961 tgtatcaatc gacgtggggc gtaagatagt ttcttctaaa gatggaaaag aatacgcgta
1021 tgaaaaattg attcttgcaa cacctgctag cgcacgtagg ttaacctgcg aggggtctga
1081 actgtctggg gtctgctatt tacgcagtat ggaagacgcc aaaaatttac gtaggaaact
1141 tgtggagagt gcgtctgttg ttgtgttggg cggcggagta atcgggcttg aagtcgcctc
1201 agctgcggtg ggcttaggga agagggtcac agtgatagaa gccaccccgc gtgtaatggc
1261 gcgcgtggtt acgccggcag cagcaaactt agtcagagcc cgcctggagg ctgaaggaat
1321 tgagttcaag ctgaatgcga aattaacgtc tataaagggc aggaatggcc atgttgaaca
1381 atgcgtactt gaaagtggag aagaaattca ggcggatctg attgtagttg gaatcggtgc
1441 tatcccagag ctagagctgg caactgaggc ggcccttgaa gtgagtaatg gtgttgtggt
1501 cgatgatcag atgtgtacat cggatacaag tatatatgca atcggcgact gcgcaatggc
1561 tagaaatcct ttttggggaa cgatggtacg tttagagaca attcataatg cggttacaca
1621 cgctcaaatt gtcgcaagta gcatctgtgg cacatcaaca ccagcaccaa ccccaccacg
1681 gttctggtct gatcttaaag ggatggcgct gcaaggactt ggtgctctaa aggactacga
1741 taaactcgtt gttgcaatta ataacgaaac tcttgaacta gaagtccttg cgtacaagca
1801 ggagcgactg attgcaactg agacaataaa tttgcctaaa cgtcaaggtg cgcttgcagg
1861 gagtataaaa ttacctgatt agcaatgatg ctcagccact cgaaccaacg gtcgcgatag
1921 ggacggcagt tacctgccgc cccccgcact ccgtacgtgc ggaactaccg cgtaaaatgt
1981 ggcccaggct gttatgtggc gcttgggcgg ggaagtattg ccatatttgg tgatgaccgt
2041 tttctacgcc acataaatcg gtggtggcta tggtgggatt tcccttgctg aaatgggaga
2101 tccgatcatg ttcgagct
//