GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS PSENOSDFY 3300 bp ds-DNA BCT 27-MAY-1991
DEFINITION Pseudomonas stutzeri nosDFY genes involved in copper processing
ACCESSION X53676
KEYWORDS ATP-binding protein; nitrous-oxide reductase; nosD gene; nosF gene;
nosY gene; nosZ gene; transmembrane protein.
SOURCE Pseudomonas stutzeri DNA.
ORGANISM Pseudomonas stutzeri
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 1 to 3300)
AUTHORS Zumft,W.G.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 3300)
AUTHORS Zumft,W.G., Viebrock-Sambale,A. and Braun,C.
TITLE Nitrous oxide reductase from denitrifying Pseudomonas stutzeri.
Genes for copper-processing and properties of the deduced products,
including a new member of the family of ATP/GTP-binding proteins
JOURNAL Eur. J. Biochem. 192, 591-599 (1990)
STANDARD full automatic
COMMENT *source: strain=ZoBell ATCC 14405 see M22628 for nosZ gene
Data kindly reviewed (10-DEC-1990) by Zumft W.
From EMBL entry PSNOSDFY; dated 12-DEC-1990.
FEATURES Location/Qualifiers
CDS <1..64
/note="nosZ product, nitrous-oxide reductase (1 is 3rd
base in codon)"
/codon_start=1
CDS 189..1496
/note="nosD product precursor"
/codon_start=189
CDS 189..269
/note="signal peptide"
/codon_start=189
CDS 270..1496
/note="mature nosD product, periplasmic protein"
/codon_start=270
CDS 1496..2419
/note="nosF product, ATP-binding protein, peripheral
location"
/codon_start=1496
CDS 2419..3246
/note="nosY product precursor"
/codon_start=2419
CDS 2419..2520
/note="signal peptide"
/codon_start=2419
CDS 2521..3246
/note="mature nosY product, transmembrane protein"
/codon_start=2521
CDS 3278..>3300
/note="ORF 4 (3300 is 2nd base in codon)"
/codon_start=3278
BASE COUNT 605 a 1011 c 1030 g 654 t
ORIGIN
1 ctgcagctgg ttctgccatg cgctgcacat ggaaatggtc ggccgcatga tggtcgagcc
61 ggcctaagct gttcgagcca tcgccaataa gccggtgata gttcagcgac tgcgcgagga
121 gcctcggtcc tgccgaggtt gttcgcgcag tgtttacccg cctggaagct gaagaaggtc
181 aaaacgcagt gttcaaagct caggctactt tctcgcggta ctcagcggca gtttcgctgc
241 tgcttttatt cagtggtgct gcccaggcgg caccgcaatc gattaccacc ttgccgctgc
301 agcccgatgg tgagaaccgc tggcgcttgc ctgccggtga gtatcaaggc cagttcacca
361 tcgagcagcc catgcagctg cggtgcgaac cgggagccgt catccagtcg caggggcagg
421 gcagcagcct gctcatcagc gcgcccgacg tcctcgtcga aggctgcacg ctgtacgaat
481 ggggctccga cctcaccgcc atggattcgg cagtattcat cctgcctgct gcggaacgtg
541 cgcagatcag caataaccgt atgcgcggcc cggggttcgg tgtgttcgtc gatggcacca
601 gagacgtgca ggtgataggc aatgaaatcg atggcgacgc cggcgtccgt tcgcaggatc
661 gcggcaacgg tattcatcta ttcgcagtca gtggcgcacg cgtgctgcac aatcacgtac
721 gcaatgcgcg cgatggcatc tacatcgata cctccaacgg caaccacctc gaaggcaacg
781 tgatcgagga tgttcgttac ggcgtgcatt acatgttcgc caacgaaaac agcctcatcg
841 acaacgtaac ccgtcgtacg cgcacgggtt atgcgttgat gcagagccgc aagctgacgg
901 tgaccggcaa ccgttccgag caggatcaga actacggcat cctgatgaac tacatcacct
961 attcgaccat taccggcaac ttcgtcagcg acgtgcagcg tggtgatacg ggtggcgaca
1021 gcatgatcag cggtggtgag ggcaaagcgc tgttcatcta caactcgctg ttcaacacca
1081 tcgaaaacaa tcacttcgaa aagagctcgc tgggcatcca cctgaccgcc ggttccgaag
1141 ataaccgcat ctccggtaac gccttcgttg gcaaccaaca gcaggtcaag tacgtcgcca
1201 gccgcaccca ggagtggtcg gtggatggcc gcggcaacta ctggagtgat tacctgggct
1261 gggatcgcaa caacgacggt ctcggcgata tcgcctacga accgaacgac aacgtcgatc
1321 gcctgctctg gctctacccg caggtgcgct tgctgatgaa cagcccgagc atcgaggtgc
1381 tgcgttgggt gcagcgggcg ttcccggtga tcaagtcacc aggcgtgcag gacagccatc
1441 cattgatgaa gctgcccact gaaaaactcc ttaccgaaaa gcaggaacca acgtcatgaa
1501 cgccgtcgag atccagggcg taagccagcg ttacggcagc atgaccgtgc tgcacgattt
1561 gaatctgaac ctcggtgaag gtgaggtgct ggggttgttc ggccataacg gcgcgggcaa
1621 gaccaccagc atgaagctga ttcttggcct gctctcaccg agcgaaggcc aggtcaaggt
1681 actcggccgc gcgccgaacg atccgcaagt gcgccgtcag ctcggctatc taccggaaaa
1741 cgtgaccttc tatccgcaac tgagcggccg cgaaaccctg cgccacttcg ctcgcctgaa
1801 gggcgcggcg ttgacccagg tggatgagct gctcgagcag gtcggcctcg cccatgccgc
1861 ggatcgccgg gtgaagacct attccaaggg catgcgccag cgcctcggtc tggcccaggc
1921 actgctcggc gagccgcgct tgctgctgct cgatgagccg accgtggggc tcgatccgat
1981 cgccacccag gatctctatc tgctgattga tcgcctgcgt cagcgcggca ccagcatcat
2041 tctttgctcc cacgtgctac cgggcgtcga ggcgcacatc aaccgggctg caattctcgc
2101 caagggctgc ctgcaggcag tgggcagcct ctcgcaattg cgtgccgagg cagggctgcc
2161 ggtgcgcatt cgcgccagtg gtatcagcga gcgggacagc tggttgcagc gctggaccga
2221 tgcaggccac agcgcacgcg ggctcagcga gtcgagcatc gaggtcgtgg ccgtcaacgg
2281 ccacaagctg gtgttgctgc gccagctgct gggtgaggga gagccggagg acatcgagat
2341 ccatcagcca tcgctggaag acctctaccg ctattacatg gagcgtgccg gcgacgtgcg
2401 cgctcaggag ggccgtctat gaaccaggtc tggaacatcg cccgcaagga actcagcgac
2461 ggcctgcgta accgctggtt gctcgccatc agcctgctct tcgcggtgct cgcggtgggc
2521 atcgcctggc tcggcgctgc cgcgtccggc caattgggct tcacttcgat tccggcaacc
2581 atcgccagcc tggcgagcct cgccaccttc ctgatgccgc tgatcgcgtt gctgctggcc
2641 tatgacgcca tcgtcggcga agacgagggc ggcacgctga tgctgctgct gacgtatccg
2701 ctggggcggg ggcagatcct gctgggcaag ttcgtcggtc acgggctgat ccttgccctg
2761 gcggtgctga tcggcttcgg ttgtgcggcg ctggccatcg cgctgctggt cgagggtgtc
2821 gagctcggca tgctgttctg ggcattcggt cgcttcatga tctcctcgac gctgcttggc
2881 tgggtgtttc tcgccttcgc ctacgtgctc agcggcaagg tcaacgagaa gtccagcgcg
2941 gcgggtctgg cgctcggcgt gtggtttctc ttcgtgctgg tctttgacct ggtactgctg
3001 gcgctgctgg tgctcagtga aggcaagttc aatcctgaac tgctgccctg gttgctgctg
3061 ctcaatccca ccgacatcta ccgcctgatc aacctgtccg gcttcgaagg cagtggtagc
3121 gccatgggcg tgttgtcgct gggtgccgat ctgccggtgc ccgccgcagt gctctggctg
3181 tgcctgctgg cctggatcgg ggtgtcgctg ctgctggcgt atgcgatctt ccgccggcgc
3241 ttgacctgac tttcatgaat cgaaaaggaa atcctctatg aacgcactgc atcgcatcgg
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