GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS RCAHUPLS 4175 bp ds-DNA BCT 27-MAY-1991
DEFINITION Rhodobacter capsulatus ORFX and hupL and hupS genes for hydrogenase
large and small subunits
ACCESSION X13520
KEYWORDS hupL gene; hupS gene; hydrogenase; hydrogenase large subunit;
hydrogenase small subunit; unidentified reading frame.
SOURCE Rhodobacter capsulatus DNA.
ORGANISM Rhodobacter capsulatus
Prokaryota; Bacteria; Gracilicutes; Anoxyphotobacteria;
Purple nonsulfur bacteria.
REFERENCE 1 (bases 490 to 3509)
AUTHORS Leclerc,M., Colbeau,A., Cauvin,B. and Vignais,P.M.
TITLE Cloning and sequencing of the genes encoding the large and the
small subunits of the H-2 uptake hydrogenase (hup) of Rhodobacter
capsulatus
JOURNAL Mol. Gen. Genet. 214, 97-107 (1988)
STANDARD full automatic
REFERENCE 2 (bases 3325 to 3405)
AUTHORS Leclerc,M., Colbeau,A., Cauvin,B. and Vignais,P.M.
TITLE Cloning and sequencing of the genes encoding the large and the
small subunits of the H(2) uptake hydrogenase (hup) of Rhodobacter
capsulatus
JOURNAL Mol. Gen. Genet. 215, 368-368 (1988)
STANDARD full automatic
REFERENCE 3 (bases 1 to 4175; 3364 to 4175)
AUTHORS Richaud,P.
JOURNAL Unpublished (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P15283; HUPS$RHOCA. SWISS-PROT; P15284; HUPL$RHOCA.
SWISS-PROT; P16145; YHUP$RHOCA.
*source: strain=B10; library=pBR322; clone=pAC70;
From EMBL entry RCHUPLS; dated 07-SEP-1990.
FEATURES Location/Qualifiers
RBS 480..484
/note="put. ribosome binding site"
CDS 490..1563
/note="put. hydrogenase small subunit preprotein (AA -45
to 313)"
/codon_start=490
CDS 490..624
/note="put. signal peptide (AA -45 to -1)"
/codon_start=490
CDS 625..1563
/note="hydrogenase small subunit (AA 1 - 313)"
/codon_start=625
RBS 1556..1561
/note="put. ribosome binding site"
CDS 1570..3360
/note="hydrogenase large subunit (AA 1 - 617)"
/codon_start=1570
CDS 3373..4158
/note="ORF X"
/codon_start=3373
BASE COUNT 847 a 1316 c 1283 g 729 t
ORIGIN
1 ctgtttcaac ttctggcggc aggcgagcgc cccgaccgtg tcgcccatgc gctgcacgcc
61 agccttgcgc aggcttttgc cgccgaagcg cggcggctga tcgaagccgg acaggccgag
121 gcggtggcgc tgacgggcgg ctgttttcag aattccagac ttgcaaccat gacgcgaaat
181 ttccttgccg atcaaggcat tctgacgcag ggccgcatcc cggccaacga tgggggcctt
241 gccctgggtc aggcccttgt cgcggcggca aaattggaaa gcaactgacc gaagtggaaa
301 tggaatcggt caaaatactt tcggtttttt tacgtgacaa aatcacacac cattgaaaaa
361 caaggaaaaa tttttctgcg acaatttgcc gtttctcatt ggcggaaacc ggcccaacca
421 tgagaattcc actctaccca tgggcgcgcc gaggaggtca cggcgcgtca caaggcaagg
481 gagggacaat tgtcggacat cgaaactttc tatgacgtga tgcggcgtca ggggatcacc
541 cggcgcagct tcatgaagtt ctgttcgctc accgcagcag ctttggggct tgggccctcc
601 ttcgtgccga aaatcgccga agccatggaa acgaagccgc gcaccccggt cgtctgggtg
661 catgggcttg aatgcacctg ctgttcggaa agcttcatcc gctcggcgca tccgctggcc
721 aaggatgtcg tgctctcgat gatctcgctc gattacgacg acacgctgat ggccgccgcc
781 ggtcacgccg ccgaagcggc cttcgaggaa accatcgcca aatacaaggg caactacatc
841 ctggccgtcg agggcaaccc gccgctcaac gaagacggga tgttctgcat caccggcggc
901 aagccctttg tcgagaagct gcgccacgcc gccgagggcg ccaaggcgat catcagctgg
961 ggggcctgtg cgtcttatgg ctgcgtgcag gccgcggcgc cgaaccccac ccaggcgacg
1021 ccggtgcaca aggtgatcac cgacaagccg atcatcaagg tcccgggctg cccgccgatc
1081 gccgaggtga tgaccggcgt catcacctac atgctgacct tcgaccggat gccggaactg
1141 gaccgtcagg gccgcccggc gatgttctac agccagcgca tccacgacaa atgctaccgc
1201 cgcccgcatt tcgacgccgg gcaattcgtc gaacactggg acgacgaaaa cgcgcgcaag
1261 ggctattgcc tctacaagat gggctgcaag ggcccgacca cctacaacgc ctgttcgacc
1321 gtgccgctgg aacggcggcg tcacttcccg atccagtccg gtcacggctg catcggctgt
1381 tccgaggacg ggttctggga tcagggcagc ttctatgacc ggctgaccac gatcaagcaa
1441 ttcggcatcg aggccacggc cgaccagatc ggctggaccg ccaccggcct tgtgggcgcc
1501 gccgtcgccg cccatgccgc ggtctccgtg ctcaaacgcg cgcagaaaaa gaacgaggag
1561 gcgtaagcca tgacgaccca aacgccgaac ggcttcaccc tcgacaacgc cggcaagcgc
1621 atcgtcgtcg atcccgtcac ccggatcgaa ggccacatgc gctgcgaagt gaacgtcaac
1681 gatcagggca tcatcaccaa cgccgtctcg accgggacga tgtggcgcgg ccttgaagtg
1741 atcctgaagg gccgcgatcc gcgcgacgcc tgggccttca ccgaacggat ctgcggtgtc
1801 tgcaccggca cccatgcgct gacctcggtc cgcgcggtcg aaagcgcgct ggggatcacc
1861 atccccgaca atgcgaattc gatccgcaac atgatgcagc tgaacctgca gatccacgac
1921 catatcgtgc atttctacca cctgcatgcg ctggattggg tgaacccggt caatgcgctg
1981 cgcgccgatc cgaaggcgac ctcggaactg cagcagatgg tttcgcccag ccatccgctg
2041 tcgtcccccg gctatttccg cgacgtgcag aaccggctga agaaattcgt cgaatccggg
2101 cagctggggc tgttcaagaa cggctactgg gacaatccgg cctacaagct gccgcccgaa
2161 gccgatctga tggcgacgac gcattatctg gaagcgctgg atctgcaaaa ggaagtggtc
2221 aaggtccaca cgatcttcgg cggcaagaac ccgcatccga actggcttgt gggcggtgtg
2281 ccctgtccga tcaacgtcga tggcgtgggc gcggtcggtg cgatcaacat ggagcggctg
2341 aacctcgtct cctcgatcat cgaccgctgc accgaattca cccgcaacgt ctatctgccc
2401 gacctcaagg ccatcggcgg cttctacaag gaatggctct atggcggcgg gctgtcgggg
2461 caatcggtgc tgtcctatgg cgacatcccg gaaaacccga atgatttcag cgccggtcag
2521 ctgcatctgc cgcgcggggc gatcatcaac ggcaacctga acgaggtgca tgacgtcgac
2581 acgaccgacc ccgaacaggt gcaggaattc gtcgaccatt cctggtatga ttacggcgag
2641 ccgggcatgg ggctgcatcc ctgggacggc cggaccgagc cgaaattcga gctgggcccg
2701 aacctgaaag gcacccgcac caacatcgag aacatcgacg aaggcgcgaa atattcctgg
2761 atcaaggcgc cgcgctggcg cggcaatgcg atggaggtgg ggccgctggc cgctacatcg
2821 tcggttacgc gcaagggcca cgaggacatc aagaaccagg tcgagggcct gctgcgcgac
2881 atgaacctgc cggtttcggc gctgttctcg acgctgggcc ggactgcggc gcgggctctg
2941 gaggcggaat actgctgccg tctgcaaaag cacttcttcg acaagctggt caccaacatc
3001 aagaacggcg acagctcgac cgcgaatgtc gagaaatggg atccctcgac ctggccgaag
3061 gaggccaagg gcgtgggcat gaccgaggcg ccgcgcggcg cgctgggcca ttgggtcaag
3121 atcaaggacg gccgcatcga gaactatcaa tgcgtcgtgc cgacgacctg gaacggctcg
3181 ccccgcgaca gcaagggcaa tatcggcgcc ttcgaggcct cgctgctgaa cacgaagatg
3241 gaacgccccg aagagccggt cgagatcctg cgcacgctgc acagcttcga tccgtgcctg
3301 gcctgttcga cgcatgtgat gtcggccgaa ggcgcccccc tgaccaccgt caaggtccgg
3361 tagggggatg ccatgaaggg agtttccgac gaaaggatca atgcccccgt ccgtggcccg
3421 gatgaaatct tcgaggcctc gcgactgacc ggcgacgcca cccgcgagga cctagaaagc
3481 atccggcggc gtacctccgt ctatgtctac gaggcgccgg tccgggtctg gcactgggtc
3541 aacgcgctgg cgatcaccat cctggtggtc accggctatt tcatcgcctc gccgctgccc
3601 tcgatgcaga tcggcgaggc caccgaccag ttcgtgatgg gctacatccg cttcgcgcat
3661 ttcgccgccg gggtggtgat gtcggtggcc tttttcggcc gcatctactg ggccttcgtg
3721 ggcaaccgcc acgcctggca gatgttctac atcccgatct tcaacaagcg ctactggaag
3781 gaattcgtct tcgagctgcg ctggtatttc ttccttgaag aagagccgaa gaaatacatc
3841 ggccacaacc cgctggcgca tgcggcgatg ttcaccttca tcaccttggg catcaccttc
3901 atgatgatca ccggctgggc gctttatgcc gaaggcgcgg ggcagggggg cgtgaccgac
3961 agcctgttcg gctgggtgct gggctatgtg caaaacagcc agcggctgca cacgttgcac
4021 catctgggca tgtgggcgat cgtgatcttc gcgatcatcc atatctacgc cgcggtgcgc
4081 gaagacgtga tgagccgcca gtcgatggtc tcgacgatga tctcggggca ccggaccttc
4141 aaggacgacc ggatcgagtg actggaaaga agctt
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