GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS RHMA9INS1 1195 bp ds-DNA BCT 27-MAY-1991
DEFINITION Rhizobium japonicum repeated DNA sequence RS(alpha) 9 insertion
sequence-like 1.6 kb upstream of nif DK operon
ACCESSION X02581
KEYWORDS direct repeat; insertion sequence; inverted repeat;
unidentified reading frame.
SOURCE Rhizobium japonicum DNA.
ORGANISM Rhizobium japonicum
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Rhizobiaceae.
REFERENCE 1 (bases 1 to 1195)
AUTHORS Kaluza,K., Hahn,M. and Hennecke,H.
TITLE Insertion sequence-like repeated sequences clustered around the nif
region of the Rhizobium japonicum genome
JOURNAL J. Bacteriol. 162, 535-542 (1985)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Goettfert,M.
JOURNAL Unpublished (1990)
STANDARD full staff_review
COMMENT Data kindly reviewed (17-APR-1986) by K. Kaluza
From EMBL entry RJA9INS1; dated 26-NOV-1990.
FEATURES Location/Qualifiers
repeat_region 16..20
/note="direct repeat 1"
repeat_region 23..27
/note="direct repeat 1"
repeat_unit 40..48
/note="inverted repeat"
repeat_region 40..43
/note="direct repeat 2, put. target site"
insertion_seq 44..1163
/note="put. insertion sequence RS alpha 9"
CDS 87..1151
/note="pot. unidentified reading frame I"
/codon_start=87
RBS 438..443
/note="pot. rRNA binding site"
old_sequence 544..548
/note="tac was ac in [1]"
/citation=[1]
old_sequence 580..584
/note="cgc was cc in [1]"
/citation=[1]
misc_feature 673..673
/note="single bp exchange in variant RS alpha"
misc_feature 831..831
/note="single bp exchange in variant RS alpha"
misc_feature 936..936
/note="single bp exchange in variant RS alpha"
misc_feature 1109..1109
/note="single bp exchange in variant RS alpha"
misc_feature 1126..1128
/note="GCC (Arg) deleted in RS (alpha) 7"
misc_feature 1132..1134
/note="GTC (Arg) deleted in RS (alpha) 10"
repeat_unit 1159..1167
/note="imp. inverted repeat"
repeat_region 1164..1167
/note="direct repeat 2; put. target site"
BASE COUNT 254 a 365 c 347 g 229 t
ORIGIN
1 ggccgcctta ggcgacccgt ctcccgtttt cctcgctcgc tagtacccag cttttcttgg
61 aacgtgagtc gtgattcaag gtcgggatga tacccgaagc aagagaagtc cacctttcga
121 ggaaagatcg caaggtgctt gaggcggtct gtcgctcacc ggtgacgttg cagcgcgatt
181 tgaagcgggc gcggatagtt ctgttggcgg cggatgggcg cagcacccgg tcgatcgcca
241 aggaagttgg ggtccagccg cggattgtca gcctttggcg gcatcgctat gccgaccatg
301 gccttgaagg gctgcaagac aagccgcggc ctggcaagca gccgatctat acgaagacga
361 ccgacaagcg gattctgaag ctgctggata agccgccacc gcaagggttt gcgcgctgga
421 ccggccccct gctggccgag gcgctgggcg atgtcgatgt ccaatatgtc tggcggttcc
481 tgcgcagcca caagattgac ctggtggctc gcaagtcctg gtgcgagagc aacgacccga
541 actttacggc caaagccgcc gatgttgtcg gcctctatgt cgcgccgccg gcgaaggcca
601 ttgtgctgtg cgtggacgag aagccctcga tccaggcttt ggagcgagcg cagggttatc
661 tgaagttgcc cagtggccgc gccttaaccg gccaaagcca cgattacaag cggcatggca
721 ccacaacatt gtttgcggcg ctcgaagtcg ccaccggaaa gatcatcgcg acccattcaa
781 aacgccggcg ccgcgtcgag tttctcgatt tcatgaacag cgtcaccgcg gcttttccga
841 accgcaagct tcacgtcatc ctcgacaacc tcaacaccca taaaaagaac gaggactggc
901 tcaaggccca ccccaacgtg caatttcatt tcacgccgac aagtgcgcca tggctcaatc
961 aggtcgaagt atggttttcc atcttgcagg ggcagtcgct cagcggcacc tccttcacga
1021 gcctcaagca gcttcaggaa cacatcgatg cctacgtcaa cgcatacaac gacagagccg
1081 agcccttcgt ctggaccaag aaaaaggtcc gtcaacgccg tttcaaaggc cgtcgccgta
1141 tcactcagct ctgattccgg ggactagccc cgtgttcgaa gagagctggc cccgg
//