GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS RHMNIFPRE 2076 bp ds-DNA BCT 27-MAY-1991
DEFINITION Rhizobium leguminosarum fixX (partial), nifA, and nifB (partial)
genes
ACCESSION X17073
KEYWORDS fixX gene; nifA gene; nifA regulatory protein; nifB gene;
regulatory protein.
SOURCE Rhizobium leguminosarum DNA.
ORGANISM Rhizobium leguminosarum
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Rhizobiaceae.
REFERENCE 1 (bases 1 to 2076)
AUTHORS Roelvink,P.W.
JOURNAL Unpublished (1989)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2076)
AUTHORS Roelvink,P.W., Hontelez,J.G., van,K.A. and van,d.B.R.C.
TITLE Nucleotide sequence of the regulatory nifA gene of Rhizobium
leguminosarum PRE: transcriptional control sites and expression in
Escherichia coli
JOURNAL Mol. Microbiol. 3, 1441-1447 (1989)
STANDARD full automatic
COMMENT *source: strain=PRE;
Data kindly reviewed (19-JUL-1990) by Roelvink P.W.
From EMBL entry RLNIFPRE; dated 25-SEP-1990.
FEATURES Location/Qualifiers
CDS <1..32
/note="fixX protein (10 AA) (1 is 2nd base in codon)"
/codon_start=1
promoter 133..155
/note="promoter"
RBS 225..229
/note="ribosome binding site"
CDS 231..1787
/note="nifA regulatory protein (AA 1-519)"
/codon_start=231
terminator 1820..1856
/note="nifA transcription terminator"
RBS 1976..1980
/note="ribosome binding site"
CDS 1987..>2076
/note="nifB protein (AA 1-30)"
/codon_start=1987
BASE COUNT 503 a 554 c 574 g 445 t
ORIGIN
1 cccggggagg gttcggggtc ctcaaattcg gatgaggacg tccctacctc cggcgggata
61 gcagcgatcg atccagctgc attagcggcg gcgattgaat gtgcatcttc taacctttcg
121 aaaggctaat ttttgcgccg tcgatagcac gtatttctaa cctaggaata tgcgttagca
181 tatttcgcgt accagggtaa aaagcagtgc accctccctt gtttgtaggc atgattaaac
241 cagaggcgcg gctccatatt ctctacgaca tctccaaaga gcttatctct tcttttcctc
301 tagacaactt gctgaaggct gccatgaacg ccctcgtcga gcatctgcga ttgcgcgacg
361 gcggaatcgt gattcacggc tcgggaggag agccttggat aaacgtacgg gctcccattg
421 gggacgacgt tcgctcacgt tctctgacga ttgaacaggc ggacgcaata gatcgtgtca
481 tcgctagcgg tgagaagcac tttgggaaaa attctgtcgt tctccccgtt aaagtaaacc
541 ggaaagcaat cggcgcattg tggattgatt tcgcgcagaa aagcggagat caggacgaaa
601 cccttctggc aatgattgcc gtcctgatcg gcttaacctg ccagcgcgat cgcgaattgt
661 gcagcgatgg cggcgcggtc gccgaggaac aacaagcagg acagattccc aaaatcaaac
721 ccaagcctca tcccacccaa ctcgacaaaa tcgactggat cgttggggag agccccgcga
781 tcaagagggt attagctacc accaatatcg tggcgcgacg aacttccgcg gtgctcttga
841 gaggagagag cggcactggc aaggaatgct ttgctagagc aatacacgca ttatcgatac
901 ggaaaagcaa ggcgtttatt aagttgaatt gcgctgcgct gtcggaaacc gttctggaat
961 ccgaattgtt tggccatgag aagggcgctt tcactggcgc tctccttcaa cgagctggac
1021 gtttcgaact ggccaatggc ggaacgctgt tgcttgatga aattggcgat gtatcaccac
1081 aattccaggc gaagttattg cgcgtgttac aggaaggcga attcgaacgt ctcggcggaa
1141 cgaagacatt gaaagtagac gttcgagtta tatgcgccac caacaaaaac cttgaagtgg
1201 ccgtccttcg aggggagttc agagccgacc tctattaccg gatcaatgtg gtgccgatca
1261 ttttgccgcc acttcggcag cgggacggag acatttcgct tctagcacaa gtgttcctcg
1321 agcaattcaa caaggcaaat gatcgaaatt gcgacttcgc cccgtcggcc atagacattt
1381 tgtcgaaatg cgccttcccc ggcaatgttc gcgagctgga caactgcgtt caaaggaccg
1441 ccactctcgc cagttcaaat accatcactt catcggattt tgcctgtcag caagaccagt
1501 gttcttcggc gctcctctgg aaagacgccc gcgacggcac tggcaacggc ccggtgcata
1561 gtctcaaccc gcgagataca atgttaggcg gactcggggc caacgtaggt actcccagcg
1621 gtgccgcagc cacaatcgag cgagcgggtc tcactgagcg tgatcggctg atcaatgcaa
1681 tggtgaaggc tggctgggta caggccaaag cggctcgtat cctgggtaaa acgccgcggc
1741 aggtcggcta tgcgctacgc cggcatcgta tcgatgtgaa gaaggagtga cagcaatcgc
1801 caagaactcc gtaagcgtgg cccaacgact tcgggcgcga tctattttca tttctacaag
1861 acatctccgg cagcaagcgg ggaaacgagc ggtaaggcga cataagcgct gaaacaataa
1921 tatctcatcg accggaatct ctcttgcttt tggagctgta acctttcctc aacatggaac
1981 agtgcgatgt gggaaccgga aataaaggtc gggacgacca gcagtgcccc ctccgaccgg
2041 gcgccgatgg ctcccgctat gcccggtggc gccgca
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