GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS STAIS431M 1223 bp ds-DNA BCT 27-MAY-1991
DEFINITION S. aureus IS431mec gene associated with methicillin resistance
ACCESSION X53818
KEYWORDS insertion sequence; methicillin resistance; putative transposase.
SOURCE Staphylococcus aureus DNA.
ORGANISM Staphylococcus aureus
Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
Micrococcaceae.
REFERENCE 1 (bases 186 to 899)
AUTHORS Barberis-Maino,L., Berger-Baechi,B., Weber,H., Beck,W.D. and
Kayser,F.H.
TITLE IS431, a staphylococcal insertion sequence-like element related to
IS26 from Proteus vulgaris
JOURNAL Gene 59, 107-113 (1987)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 1223)
AUTHORS Barberis-Maino,L.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 3 (bases 1 to 1223)
AUTHORS Barberis-Maino,L., Ryffel,C., Kayser,F.H. and Berger-Baechi,B.
TITLE Complete nucleotide sequence of IS431mec in Staphylococcus aureus
JOURNAL Nucleic Acids Res. 18, 5548-5548 (1990)
STANDARD full automatic
COMMENT *source: strain=NCTC8325 mec; isolate=BB270. **map:mec specific DNA
or mec determinant. See <X52594> (SAHVR) and <M18438> (TT431MEC)
[2] for overlapping sequences.
From EMBL entry SAIS431M; dated 12-NOV-1990.
FEATURES Location/Qualifiers
old_sequence 188..188
/note="t was c in [2]"
/citation=[1]
old_sequence 214..216
/note="acg was ag [2]"
/citation=[1]
repeat_unit 215..231
/note="inverted repeat (IR-L) A"
old_sequence 241..241
/note="a was r in [2]"
/citation=[1]
old_sequence 245..247
/note="taa was ta in [2]"
/citation=[1]
CDS 272..943
/note="putative transposase (AA 1 - 224)"
/codon_start=272
old_sequence 303..303
/note="a was r in [2]"
/citation=[1]
old_sequence 318..320
/note="tag was tg in [2]"
/citation=[1]
repeat_unit 990..1006
/note="inverted repeat (IR-R) A'"
BASE COUNT 431 a 201 c 188 g 403 t
ORIGIN
1 tctagattct agcatattta agtgtttctc tttatctaat gctttgtcat atcctttaac
61 gattgaacca ctaaagattt ctcctactgc tcctgaacca taactaaata gacatacttc
121 tcttctggtt ggaatgtgtg gttctgtaat aacgaaatta aacttaagta taatgatcct
181 gtataaatgt taccaacatc tctattccat aatacggttc tgttgcaaag ttgaatttat
241 agtataattt taacaaaaag gagtcttctg tatgaactat ttcagatata aacaatttaa
301 caaggatgtt atcactgtag ccgttggcta ctatctaaga tatacattga gttatcgtga
361 tatatctgaa atattaaggg aacgtggtgt aaacgttcat cattcaacgg tctaccgttg
421 ggttcaagaa tatgccccaa ttttgtatca aatttggaag aaaaagcata aaaaagctta
481 ttacaaatgg cgtattgatg agacgtacat caaaataaaa ggaaaatgga gctatttata
541 tcgtgccatt gatgcagagg gacatacatt agatatttgg ttgcgtaagc aacgagataa
601 tcattcagca tatgcgttta tcaaacgtct cattaaacaa tttggtaaac ctcaaaaggt
661 aattacagat caggcacctt caacgaaggt agcaatggct aaagtaatta aagcttttaa
721 acttaaacct gactgtcatt gtacatcgaa atatctgaat aacctcattg agcaagatca
781 ccgtcatatt aaagtaagaa agacaaggta tcaaagtatc aatacagcaa agaatacttt
841 aaaaggtatt gaatgtattt acgctctata taaaaagaac cgcaggtctc ttcagatcta
901 cggattttcg ccatgccacg aaattagcat catgctagca agttaagcga acactgacat
961 gataaattag tggttagcta tattttttta ctttgcaaca gaaccgaaaa taatctcttc
1021 aatttatttt tatatgaatc ctgtgactca agattgtaat atctaaagat ttcagttcat
1081 catagacaat gttcttttca acatttttta tagcaaattg attaaataaa ttctctaatt
1141 tctcccgttt gatttcacta ccatagatta tattatcatt gatatagtca atgaataatg
1201 tacaaattat cactcataac agt
//