GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS STAMECAPB 2456 bp ds-DNA BCT 27-MAY-1991
DEFINITION S. aureus mecA gene for PBP2' (penicillin binding protein 2')
ACCESSION X52593
KEYWORDS mecA gene; methicillin resistance; penicillin-binding protein;
penicillin-binding protein 2.
SOURCE Staphylococcus aureus DNA.
ORGANISM Staphylococcus aureus
Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
Micrococcaceae.
REFERENCE 1 (bases 1 to 2456)
AUTHORS Ryffel,C.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2456)
AUTHORS Ryffel,C., Tesch,W., Birch-Machin,I., Reynolds,P.E.,
Barberis-Maino,L., Kayser,F.H. and Berger-Baechi,B.
TITLE Sequence comparison of mecA genes isolated from
methicillin-resistant Staphylococcus aureus and staphylococcus
epidermidis
JOURNAL Gene 94, 137-138 (1990)
STANDARD full automatic
COMMENT *source: strain=NCTC8325, isolate=BB270; library=EMBL-3; See also
<X52592>,<X52594> and <Y00688>. Data kindly reviewed (23-JUL-1990)
by C. Ryffel
From EMBL entry SAMECAPB; dated 21-JAN-1991.
FEATURES Location/Qualifiers
promoter 80..85
/note="-35 region"
promoter 101..105
/note="-10 region"
RBS 130..134
/note="ribosome binding site"
precursor_RNA 141..2153
/note="primary transcript"
CDS 141..2144
/note="PBP2' (AA 1-668)"
/codon_start=141
BASE COUNT 1001 a 344 c 396 g 715 t
ORIGIN
1 atgaactgat tatacttaac attaaaaaag atgataacac cttctacacc tccatatcac
61 aaaaaattat aacattattt tgacataaat actacatttg taatatacta caaatgtagt
121 cttatataag gaggatattg atgaaaaaga taaaaattgt tccacttatt ttaatagttg
181 tagttgtcgg gtttggtata tatttttatg cttcaaaaga taaagaaatt aataatacta
241 ttgatgcaat tgaagataaa aatttcaaac aagtttataa agatagcagt tatatttcta
301 aaagcgataa tggtgaagta gaaatgactg aacgtccgat aaaaatatat aatagtttag
361 gcgttaaaga tataaacatt caggatcgta aaataaaaaa agtatctaaa aataaaaaac
421 gagtagatgc tcaatataaa attaaaacaa actacggtaa cattgatcgc aacgttcaat
481 ttaattttgt taaagaagat ggtatgtgga agttagattg ggatcatagc gtcattattc
541 caggaatgca gaaagaccaa agcatacata ttgaaaattt aaaatcagaa cgtggtaaaa
601 ttttagaccg aaacaatgtg gaattggcca atacaggaac acatatgaga ttaggcatcg
661 ttccaaagaa tgtatctaaa aaagattata aagcaatcgc taaagaacta agtatttctg
721 aagactatat caacaacaaa tggatcaaaa ttgggtacaa gatgatacct tcgttccact
781 ttaaaaccgt taaaaaaatg gatgaatatt taagtgattt cgcaaaaaaa tttcatctta
841 caactaatga aacagaaagt cgtaactatc ctctagaaaa agcgacttca catctattag
901 gttatgttgg tcccattaac tctgaagaat taaaacaaaa agaatataaa ggctataaag
961 atgatgcagt tattggtaaa aagggactcg aaaaacttta cgataaaaag ctccaacatg
1021 aagatggcta tcgtgtcaca atcgttgacg ataatagcaa tacaatcgca catacattaa
1081 tagagaaaaa gaaaaaagat ggcaaagata ttcaactaac tattgatgct aaagttcaaa
1141 agagtattta taacaacatg aaaaatgatt atggctcagg tactgctatc caccctcaaa
1201 caggtgaatt attagcactt gtaagcacac cttcatatga cgtctatcca tttatgtatg
1261 gcatgagtaa cgaagaatat aataaattaa ccgaagataa aaaagaacct ctgctcaaca
1321 agttccagat tacaacttca ccaggttcaa ctcaaaaaat attaacagca atgattgggt
1381 taaataacaa aacattagac gataaaacaa gttataaaat cgatggtaaa ggttggcaaa
1441 aagataaatc ttggggtggt tacaacgtta caagatatga agtggtaaat ggtaatatcg
1501 acttaaaaca agcaatagaa tcatcagata acattttctt tgctagagta gcactcgaat
1561 taggcagtaa gaaatttgaa aaaggcatga aaaaactagg tgttggtgaa gatataccaa
1621 gtgattatcc attttataat gctcaaattt caaacaaaaa tttagataat gaaatattat
1681 tagctgattc aggttacgga caaggtgaaa tactgattaa cccagtacag atcctttcaa
1741 tctatagcgc attagaaaat aatggcaata ttaacgcacc tcacttatta aaagacacga
1801 aaaacaaagt ttggaagaaa aatattattt ccaaagaaaa tatcaatcta ttaaatgatg
1861 gtatgcaaca agtcgtaaat aaaacacata aagaagatat ttatagatct tatgcaaact
1921 taattggcaa atccggtact gcagaactca aaatgaaaca aggagaaagt ggcagacaaa
1981 ttgggtggtt tatatcatat gataaagata atccaaacat gatgatggct attaatgtta
2041 aagatgtaca agataaagga atggctagct acaatgccaa aatctcaggt aaagtgtatg
2101 atgagctata tgagaacggt aataaaaaat acgatataga tgaataacaa aacagtgaag
2161 caatccgtaa cgatggttgc ttcactgttt tattatgaat tattaataag tgctgttact
2221 tctcccttaa atacaatttc ttcattttca ttgtatgttg aaagtgacac tgtaacgagt
2281 ccattttctt tttttatgga tttcttattt gtaatttcag cgataacgta caatgtatta
2341 cctggtatac agtttaataa atttaacgtt attcatttgt gttcctgcta caacttcttc
2401 tccgtattta ccttcttcta cccataattt aaatgatatt gaaagtgtat gcatgc
//