GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS STASPCG 1241 bp ds-DNA BCT 27-MAY-1991
DEFINITION S. aureus Tn554 spc gene for sp adenyltransferase AAD(9) (sp =
spectinomycin)
ACCESSION X02588
KEYWORDS adenyltransferase; spectinomycin resistance; terminator;
transferase; transposon.
SOURCE Staphylococcus aureus DNA.
ORGANISM Staphylococcus aureus
Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
Micrococcaceae.
REFERENCE 1 (bases 1 to 1241)
AUTHORS Murphy,E.
TITLE Nucleotide sequence of a spectinomycin adenyltransferase AAD(9)
determinant from Staphylococcus aureus and its relationship to
AAD(3'')(9)
JOURNAL Mol. Gen. Genet. 200, 33-39 (1985)
STANDARD full automatic
COMMENT SWISS-PROT; P04827; S3AD$STAAU.
Data kindly reviewed (02-APR-1986) by E. Murphy
From EMBL entry SASPCG; dated 06-JUL-1989.
FEATURES Location/Qualifiers
CDS <1..177
/note="3' end of tnpC"
/codon_start=1
promoter 143..148
/note="put. -35 region"
promoter 166..171
/note="put. -10 region"
precursor_RNA 187..1229
/note="spc transcript"
RBS 320..325
/note="put. rRNA binding site"
CDS 331..1110
/note="put. AAD(9) determinant (aa 1-260)"
/codon_start=331
misc_signal 1189..1235
/note="pot. transcription terminator"
repeat_unit 1189..1206
/note="inverted repeat"
repeat_unit 1216..1235
/note="inverted repeat"
misc_feature complement(1239..1241)
/note="ermA stop codon"
BASE COUNT 457 a 174 c 242 g 368 t
ORIGIN
1 gaatcccttc gtgagcgtca aataacagca aatgtagtct caaaacccaa gaaaagttct
61 cgttcggagg aaatccttat taaaacctta aaaagaagag taatggaatt agaaaaagaa
121 aataaaaaat tacagaacca aattcaaaaa ttatatggag atctgtataa taaagaataa
181 ttattaatct gtagacaaat tgtgaaagga tgtacttaaa cgctaacggt cagctttatt
241 gaacagtaat ttaagtatat gtccaatcta gggtaagtaa attgagtatc aatataaact
301 ttatatgaac ataatcaacg aggtgaaatc atgagcaatt tgattaacgg aaaaatacca
361 aatcaagcga ttcaaacatt aaaaatcgta aaagatttat ttggaagttc aatagttgga
421 gtatatctat ttggttcagc agtaaatggt ggtttacgca ttaacagcga tgtagatgtt
481 ctagtcgtcg tgaatcatag tttacctcaa ttaactcgaa aaaaactaac agaaagacta
541 atgactatat caggaaagat tggaaatacg gattctgtta gaccacttga agttacggtt
601 ataaatagga gtgaagttgt cccttggcaa tatcctccaa aaagagaatt tatatacggt
661 gagtggctca ggggtgaatt tgagaatgga caaattcagg aaccaagcta tgatcctgat
721 ttggctattg ttttagcaca agcaagaaag aatagtattt ctctatttgg tcctgattct
781 tcaagtatac ttgtctccgt acctttgaca gatattcgaa gagcaattaa ggattctttg
841 ccagaactaa ttgaggggat aaaaggtgat gagcgtaatg taattttaac cctagctcga
901 atgtggcaaa cagtgactac tggtgaaatt acctcgaaag atgtcgctgc agaatgggct
961 atacctcttt tacctaaaga gcatgtaact ttactggata tagctagaaa aggctatcgg
1021 ggagagtgtg atgataagtg ggaaggacta tattcaaagg tgaaagcact cgttaagtat
1081 atgaaaaatt ctatagaaac ttctctcaat taggctaatt ttattgcaat aacaggtgct
1141 tacttttaaa actactgatt tattgataaa tattgaacaa tttttgggaa gaataaagcg
1201 tcctcttgtg aaattagaga acgctttatt actttaattt a
//