GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS STMGYLAR 1675 bp ds-DNA BCT 27-MAY-1991
DEFINITION Streptomyces coelicolor gylR gene and gylABX operon promoter region
ACCESSION X14188
KEYWORDS activator protein; glycerol kinase; glycerol operon; gylA gene;
gylABX operon; gylR gene; kinase.
SOURCE Streptomyces coelicolor DNA.
ORGANISM Streptomyces coelicolor
Prokaryota; Bacteria; Firmicutes; Streptomycetaceae.
REFERENCE 1 (bases 1 to 1675)
AUTHORS Smith,C.P. and Chater,K.F.
TITLE Structure and regulation of controlling sequences for the
Streptomyces coelicolor glycerol operon
JOURNAL J. Mol. Biol. 204, 569-580 (1988)
STANDARD full automatic
COMMENT .
From EMBL entry SCGYLAR; dated 20-SEP-1990.
FEATURES Location/Qualifiers
precursor_RNA 273..1195
/note="gylR primary transcript"
RBS 318..322
/note="pot. ribosome binding site"
CDS 333..1094
/note="GylR protein"
/codon_start=333
repeat_unit 463..468
/note="inverted repeat A"
repeat_unit 472..477
/note="inverted repeat B"
repeat_unit 479..484
/note="inverted repeat A'"
RBS 481..486
/note="pot. ribosome binding site"
repeat_unit 482..487
/note="inverted repeat B'"
repeat_unit 1139..1150
/note="inverted repeat C"
repeat_unit 1150..1155
/note="inverted repeat D"
repeat_unit 1154..1165
/note="inverted repeat C'"
repeat_unit 1164..1169
/note="inverted repeat D'"
repeat_unit 1171..1176
/note="inverted repeat E"
repeat_unit 1180..1185
/note="inverted repeat E'"
precursor_RNA 1287..>1675
/note="gylA primary transcript"
misc_feature 1337..1337
/note="alt. gylA transcriptional start site"
misc_feature 1340..1340
/note="alt. gylA transcriptional start site"
RBS 1421..1429
/note="pot. ribosome binding site"
CDS 1436..1675
/note="GlyA protein"
/codon_start=1436
BASE COUNT 264 a 574 c 613 g 224 t
ORIGIN
1 ggggagaacg cacgccgcca gggtagccgg gaccgggggc ctatggtcag ggcgtccacg
61 agacggacga ggatggcggg gcgaataccc gccgaacgga cgagcctccc cggtccggaa
121 cgtcccactc cggcggtggc ccgcgtccgg cgcacgtgtg gggcgcgcac gcctcgtacg
181 gaacgttcgc ggtcaccggg aggtggccga agtgcaacag ttgcccgccg accattggcg
241 ggaggtcggc atggaccggt agtgttcggc attgccgaac gacgtcagcg acgccgaggt
301 cggcagtcga aggggacgga ggcagtacgt cgatggcacg gaacatccag tcgctcgagc
361 gggcggccgc catgctgcgc ctgctcgcgg gcggcgagcg gcggctcggc ctgtcggaca
421 tcgcctcgtc gctgggtctg gccaaaggca ccgcgcacgg catcctgcgc accctccagc
481 aggaggggtt cgtggagcag gacgacgcct ccgggcgcta ccagctgggc gccgagctgc
541 tgcgcctggg cacgacctac ctggacgtgc acgaactgcg ggcccgcgcc ctggtgtgga
601 ccgacgacct ggcccgctcc agcggtgaga gcgtccacct cggcgtcctg caccagcagg
661 gcgtcctgat cgtgcaccac gtcttccgcc ccgacgacag ccggcaggtc ctggagatcg
721 gggccatgca gccgctgcac tccacggcgc tgggcaaggt gctgtcggcg tacgacccgg
781 tcgcgcacag cgaggcactg gaggccgacc gcaaggcgtt caccgaccgg accgtctgcg
841 aaccggacag cttcgagcac gtcctggaca tcacgcgcgc gcgggggtac gccgccgacg
901 tcgaggagac ctgggagggc atcgcctcca tcgccgcccc catccacgac cgcaggcgga
961 tgccggtcgg cgcggtcggc atcaccgggg ccgtggaacg gctgtgccgg gagggcgagc
1021 tgcggcccga gctggtcgcg gcggtgcggg actgcgcccg cgcggtgtcg cgggacctgg
1081 gcgccgggcg gttctagggc gcgggcgggc cgacacgggc cgtacgggaa gacacagggg
1141 ccgggacacc gaaggtgttc cggccttcga cgttcccgag gaacgggccg ggcgggagat
1201 cgatagccca cgacgatcag taacgatcgt gctttcgata acacagctct tgacgcgcgc
1261 gtgacgtcga acgagactcg cgtccatcgg tcggcattgt cgaacaccta ccggcaatac
1321 gcgttagagt gtccacagtg ccaagggccg ccatcgctct cacaacgagg gcgccggaac
1381 gcccggaggg acccggggtt cggctttccc tggacgaagg acaaaggagt cgcgggtgtc
1441 cagctccgac atcttcatcg gcgagaccat cggtaccgcc ctgctcatcc tgctcggcgg
1501 cggcgtgtgt gccgccgtca cgctcaaggc ctccaaggcc cgcaacgccg gctggctcgc
1561 catcgccttc gggtggggct tcgccgtcat gacggcggcg tacatatcgg gtccgctctc
1621 cggcgcccac ctcaacccgg ccgtgaccgt cggcatcgcg atcaaggacg gcgac
//