GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS SERERYA 9769 bp ds-DNA BCT 27-MAY-1991
DEFINITION S. erythraea eryA gene for 6-deoxyerythronolide B synthase
ACCESSION X56107
KEYWORDS eryA gene; erythromycin.
SOURCE Saccharopolyspora erythraea DNA.
ORGANISM Saccharopolyspora erythraea
Prokaryota; Bacteria; Firmicutes; Nocardioform actinomycetes.
REFERENCE 1 (bases 1 to 9769)
AUTHORS Leadlay,P.F.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Cortes,J., Haydock,S.F., Roberts,G.A., Bevitt,D.J. and Leadlay,P.F.
TITLE An unusually large multifunctional polypeptide in the
erythromycin-producing polyketide synthase of Saccharopolyspora
erythraea
JOURNAL Nature 348, 176-178 (1990)
STANDARD full staff_review
COMMENT *source: strain=NRRL 2338 (sub-species).
From EMBL entry SEERYA; dated 19-NOV-1990.
FEATURES Location/Qualifiers
mRNA <57..>9593
mat_peptide 57..9590
/product="6-deoxyerythronolide B synthase"
/gene="eryA"
CDS 57..9593
/product="6-deoxyerythronolide B synthase"
/gene="eryA"
/codon_start=57
misc_feature 190..204
/note="beta-ketoacyl-ACP-synthase active site"
misc_feature 1246..1263
/note="acyltransferase active site"
misc_feature 1246..1262
/note="beta-ketoacyl-ACP-synthase active site"
misc_feature 1428..1442
/note="ACP active site"
misc_feature 1658..1671
/note="beta-ketoacyl-ACP-synthase active site"
misc_feature 2111..2125
/note="acyltransferase active site"
misc_feature 2683..2700
/note="beta-ketoacyl-ACP-synthase active site"
misc_feature 2857..2869
/note="ACP active site"
misc_feature 3029..3043
/note="acyltransferase active site"
BASE COUNT 1224 a 3467 c 3741 g 1337 t
ORIGIN
1 acgacgagct gttctcgatg ctcgaccagc ggttcggcgg gggagaggac ctgtagatga
61 gcggtgacaa cggcatgacc gaggaaaagc tccggcgcta cctcaagcgc accgtcaccg
121 agctcgactc ggtgaccgcg cgcctgcgtg aagtcgagca ccgggccggt gagccgatcg
181 cgatcgtcgg catggcgtgc cggttccccg gcgacgtgga ctcgccggag tcgttctggg
241 agttcgtgtc cggcggcggg gacgccatcg cggaggcccc cgccgaccgc ggctgggagc
301 cggaccccga cgcgcggctg ggcgggatgc tcgcggccgc gggcgacttc gacgcgggct
361 tcttcgggat ctcgccgcgc gaggcgctgg cgatggaccc gcagcagcgg atcatgctgg
421 agatctcgtg ggaggcgctg gagcgcgccg gccacgatcc ggtgtccctg cgcggcagcg
481 cgaccggggt gttcaccggt gtcggcaccg tggactacgg cccgcgaccc gacgaggccc
541 cggacgaggt cctgggctac gtcggcaccg gcaccgcctc cagcgtcgcc tccggccggg
601 tcgcctactg cctgggcctg gaaggcccgg cgatgaccgt cgacaccgcc tgttcctccg
661 ggctcaccgc cctgcacctg gcgatggagt cgctgcgccg ggacgagtgc ggcctggcgc
721 tggccggcgg cgtgacggtg atgagcagtc ccggggcgtt caccgagttc cgcagccagg
781 gcgggctcgc cgccgacggc cgctgcaagc cgttctcgaa ggccgccgac gggttcggcc
841 tggccgaggg tgccggggtc ctggtgctgc aacggctgtc ggccgcgcgg cgggagggca
901 gaccggtgct ggccgtgctg gcgggctcgg cggtcaacca ggacggcgcc agcaacgggc
961 tgaccgcgcc gagcggaccc gcgcagcagc gggtcatccg ccgggcgctg gagaacgccg
1021 gtgtccgggc gggcgacgtc gactacgtgg aggcccacgg caccggcacc aggctgggcg
1081 accccatcga ggtgcacgcg ctgctctcga cctacggcgc ggaacgcgac ccggacgatc
1141 cactgtggat cggttcggtc aagtccaaca tcggccacac ccaggccgcc gccggcgtcg
1201 ccggggtgat gaaggcggtg ctggcgctgc ggcacggcga gatgccgcgc acgctgcact
1261 tcgacgagcc ctcgccgcag atcgagtggg acctgggcgc ggtgtcggtg gtgtcgcagg
1321 cgcggtcgtg gcccgccggc gagaggcccc gcagggcggg cgtctcctcg ttcggcatca
1381 gcggcaccaa cgcgcacgtc atcgtcgaag aggcgcccga ggccgacgag cccgagccgg
1441 cacccgactc gggtccggtc ccgctggtgt tgtccggccg cgacgagcag gcgatgcggg
1501 cgcaggcggg acggctggca gaccacctcg ccgcgagccg cggaactcgt tgcgcgacac
1561 cggtttcacg ctggccaccc gccgcagcgc cgtgggagca ggtggtggtc ggcgaccgcg
1621 acgaggccct cgccgggctg cgcgcggtgg ccgaccgccg catcgccgac cggacggcca
1681 ccgggcaggg cccgaactcg ccgcgccgcg tcgcgatggt gttccccggc cagggcgcgc
1741 agtggcaggg gatggcccgc gacctgctgc gggagtcgca ggtattcgcc gactcgatcc
1801 gcgactgcga gcgggcgctg gccccgcacg tcgactggtc gctgaccgac ctgctcagcg
1861 gcgcgcgacc gctggaccgg gtcgacgtcg tccagcccgc gctcttcgcc gtcatggtgt
1921 cgctggcggc gctgtggcgc tcccacggcg tcgagcccgc cgcggtcgtc ggccactcgc
1981 agggcgagat cgccgccgcg cacgtcgccg gcgcgctcac cctggaggac gccgccaagc
2041 tcgtcgcggt ccggagccgg gtcctgcgcc ggctcggcgg ccagggcggc atggcgtcgt
2101 tcgggctggg caccgagcag gcggccgaac ggatcgggcg cttcgcgggc gcgctctcca
2161 tcgcctcggt caacggcccc cggtcggtcg tcgtcgtcgc gggggagagc gggccgctgg
2221 acgagctgat cgccgagtgc gaggccgaag cgcataaggc gcgccgcatc cccgtcgact
2281 acgcctccca ctcaccgcag gtggagtcgc tgcgcgagga gctgctgacc gagctggcgg
2341 gcatctcccc ggtgtcggcg gacgtggcgc tctactcgac cacgaccggg cagcccatcg
2401 acaccgccac gatggacacc gcctactggt acgcgaacct gcgcgagcag gtccgcttcc
2461 aggacgcgac gcggcagctc gccgaggcgg ggttcgacgc gttcgtcgag gtcagcccgc
2521 atccggtgct gaccgtcggc atcgaggcca cgctggactc cgcgctcccg gccgacgccg
2581 gcgcctgcgt cgtgggcacc ctgcggcggg accgcggcgg cctggccgac ttccacaccg
2641 cgctcggcga ggcgtacgcg cagggcgtgg aggtcgactg gagccccgcc ttcgccgacg
2701 cgcggccggt cgagctgccc gtctacccgt tccagcgcga gctgcccgtc tacccgttcc
2761 agcggcagcg gtactggctg cccatcccca ccggcgggcg cgcacgggac gaggacgacg
2821 actggcgcta ccaggtcgta tggcgggaag ccgagtggga gagcgcttcg ctggccggac
2881 gcgtgctgct ggtgaccgga ccgggcgtgc cgtccgagtt gtcggacgcc atccgaagtg
2941 gactggagca gagcggtgcg acggtcctga cctgcgacgt ggaatcccgt tcgaccatcg
3001 gcaccgcact ggaggccgcc gacaccgacg ctctgtccac tgtgggtgtc gctgctgtcc
3061 cgcacggcga ggccgtcgat ccgtcgctgg acgcgctcgc cctggtccag gccctcggag
3121 cggccggggt cgaagcaccg ctgtgggtgc tgacccgcaa cgccgtgcag gtggccgacg
3181 gcgaactggt cgatccggcg caggccatgg tgggcggtct cggccgcgtg gtcggcatcg
3241 agcagccggg gcgctggggc ggtctggtgg acctggtcga cgccgatgcc gcgtcgatcc
3301 ggtcgctggc cgcggtgctg gcggacccgc gcggcgagga gcaggtcgcg atccgggcgg
3361 acgggatcaa ggtggcgagg ctcgtgcccg cccccgcccg cgcccgcacg cacccgctgg
3421 agcccctcgc cggcaccgtg ctggtcaccg gagggatcgg cgcgcacctc gcccgctggc
3481 tggcccgctc gggcgccgag cacctggtgc tgctgggcag gcgcggtgcc gacgcacccg
3541 gcgcgtccga gctgagggag gagctgaccg cgctcggcac gggcgtgacc atcgccgcct
3601 gcgacgtcgc cgaccgggcg cggctcgaag cggtgctcgc ggcggaggcg gcggccgagg
3661 gacgcacggt cagcgccgtg atgcacgcgg cgggggtttc cacgtccacg cccctcgacg
3721 acctcaccga agccgagttc accgagatcg ccgacgtgaa ggtgcgcggc accgtcaacc
3781 tggacgagct ctgcccggac ctcgacgcgt tcgtgttgtt ctcctccaac gcgggcgtgt
3841 ggggcagtcc ggggctcgcc tcctacgcgg cggccaacgc cttcctcgac ggcttcgcgc
3901 gcgcggcgcg gagcgagggc gcgccggtga cgtccatcgc ctgggggctc tgggccgggc
3961 agaacatggc cggggacgag ggcggcgagt acctgcgcag ccagggcctg cgggccatgg
4021 acccggatcg ggccgtcgag gaactgcaca tcaccctcga ccacggtcag acgtccgtgt
4081 cggtcgtgga catggatcgc aggcggttcg tcgagctgtt caccgcggcc cggcaccggc
4141 cgctgttcga cgagatcgcc ggtgcccggg cggaagcccg gcagagcgag gagggcccgg
4201 cgctcgccca gcggctcgcg gcgctgctgt gcgacggccg agagcgcgag cacctcgccc
4261 acctgatccg cgccgaggtc gccgcggtgc tcggccacgg cgacgacgcg gcgatcgacc
4321 gcgaccgcgc cttccgcgac ctcggcttcg actccatgac cgccgtcgac ctgcggaacc
4381 ggctcgccgc ggtgaccggg gtgcgggaag ccgcgacggt ggtcttcgac cacccgacca
4441 tcacccggct cgccgaccac tacctggagc ggctcgtcgg cgcagcagag gcggagcaag
4501 ccccggcgct cgtgcgcgag gtgccgccga aggatgccga cgacccgatc gcgatcgtcg
4561 gcatggcctg ccgcttcccc ggcggcgtgc acaaccccgg tgagctgtgg gagttcatcg
4621 tcggcggcgg agacgccgtg acggagatgc ccaccgaccg cggctgggac ctcgacgcgc
4681 tgttcgaccc cgacccgcag cgccacggaa ccagctactc gcgacacggc gcgttcctcg
4741 acggggccgc cgacttcgac gcggcgttct tcgggatctc gccgcgcgag gcgctggcga
4801 tggacccgca gcagcgccag gtcctggaaa cgacgtggga gctgttcgag aacgccggca
4861 tcgacccgca ctccgtgcgg ggcagcgaca ccggcgtctt cctcggcgcc gcgtaccagg
4921 gctacggcca ggacgcggtg gtgcccgagg acagcgaggg ctacctgctc accggcaact
4981 cctccgccgt ggtgtccggc cgggtcgcct acgtgctggg gctggaaggg cccgcggtca
5041 cggtggacac ggcgtgttcg tcgtcgttgg tggccttgca ttcggcgtgt gggtcgttgc
5101 gtgacggtga ctgcggtctt gcggtggccg gtggtgtgtc ggtgatggcg ggcccggagg
5161 tgttcaccga gttctcccgc cagggcggct tggccgtgga cgggcgctgc aaggcgttct
5221 ccgcggaggc cgacggcttc ggtttgcccg agggcgtcgc ggtggtccag ctccagcggt
5281 tgtccgacgg cccggccgag ggcggtcgcc aggtgctcgg cgtggtcgcg ggctcggcga
5341 tcaaccagga cggcgcgacg aacggtctcg cggcgccgag cggcgtcgcc cagcagcgcg
5401 tgatccgcaa ggcgtgggcg cgtgcgggga tcacgggcgc ggatgtggcc gtggtggagg
5461 cgcatgggac cggtacgcgg ctgggcgatc cggtggaggc gtcggcgttg ctggctactt
5521 acggcaagtc gcgcgggtcg tcgggcccgg tgctgctggg ttcggtgaag tcgaacatcg
5581 gtcacgcgca ggcggccgcg ggtgtcgcgg gcgtgatcaa ggtggtcctg gggttgaacc
5641 gcggcctggt gccgccgatg ctctgccgcg gcgagcggtc gccgctgatc gaatggtcct
5701 cgggtggtgt ggaacttgcc gaggccgtga gcccgtggcc tccggccgcg gacggggtgc
5761 gccgggccgg tgtgtcggcg ttcggggtga gcgggacgaa cgcgcacgtg atcatcgccg
5821 agcccccgga gcccgagccg ctgccggaac ccggaccggt gggcgtgctg gccgctgcga
5881 actcggtgcc cgtactgctg tcggccagga ccgagaccgc gttggcagcg caggcgcggc
5941 tcctggagtc cgcagtggac gactcggttc cgttgacggc attggcttcc gcgctggcca
6001 ccggacgcgc ccacctgccg cgtcgtgcgg cgttgctggc aggcgaccac gaacagctcc
6061 gcgggcagtt gcgagcggtc gccgagggcg ttgcggctcc cggtgccacc accggaaccg
6121 cctccgccgg cggcgtggtt ttcgtcttcc caggtcaggg tgctcagtgg gagggcatgg
6181 cccggggctt gctctcggtc cccgtcttcg ccgagtcgat cgccgagtgc gatgcggtgt
6241 tgtcggaggt ggccgggttc tcggcctccg aagtgctgga gcagcgtccg gacgcgccgt
6301 cgctggagcg ggtcgacgtc gtacagccgg tgttgttctc cgtgatggtg tcgctggcgc
6361 ggctgtgggg cgcttgcgga gtcagcccct cggccgtcat cggccattcg cagggcgaga
6421 tcgccgccgc ggtggtggcc ggggtgttgt cgctggagga cggcgtgcgc gtcgtggccc
6481 tgcgcgcgaa ggcgttgcgt gcgctggcgg gcaagggcgg catggtctcg ttggcggctc
6541 ccggtgaacg cgcccgcgcg ctgatcgcac cgtgggagga ccggatctcc gtcgcggcgg
6601 tcaactcccc gtcctcggtc gtggtctccg gcgatccgga ggcgctggcc gaactcgtcg
6661 cacgttgcga ggacgagggc gtgcgcgcca agacgctccc ggtggactac gcctcgcact
6721 cccgccacgt cgaggagatc cgcgagacga tcctcgccga cctcgacggc atctccgcgc
6781 ggcgtgccgc catcccgctc tactccacgc tgcacggcga acggcgcgac atgggtccgc
6841 ggtactggta cgacaacctg cgctcccagg tgcgcttcga cgaggcggtc tcggcgcagt
6901 cgcccgacgg tcacgccacc ttcgtcgaga tgagcccgca cccggtgctc accgcggcgg
6961 tgcaggagat cgccgcggac gccgtggcca tcgggtcgct gcaccgcgac accgcggagg
7021 agcacctgat cgccgagctc gcccgggcgc acgtgcacgg cgtggccgtg gactggcgga
7081 acgtcttccc ggcggcacct ccggtggcgc tgcccaacta cccgttcgag ccccagcggt
7141 actggctcgc gccggaggtg tccgaccagc tcgccgacag ccgctaccgc gtcgactggc
7201 gaccgctggc caccacgccg gtggacctgg aaggcggctt cctggtccac gggtccgcac
7261 cggagtcgct gaccagcgca gtcgagaagg ccggaggcgt cgtgccggtc gcctcggccg
7321 accgcgaagc gctcgcggcg gccctgcggg aggtgccggg cgaggtcgcc ggcgtgctct
7381 cggtccacac cggcgccgcc aacgccctcg ccctgcacca gtcgctgggt gaggccggcg
7441 tgcgggcccc gctctggctg gtcaccagcc gagcggtcgc gctcggggag tccgagccgg
7501 tcgatcccga gcaggcgatg gtgtggggtc tcgggcgcgt catgggcctg gagaccccgg
7561 aacggtgggg cggtctggtg gacctgcccg ccgaacccgc gccgggggac ggcgaggcgt
7621 tcgtcgcctg cctcggcgcg gacggccacg aggaccaggt cgcgatccgt gaccacgccc
7681 gctacggccg ccgcctcgtc cgcgccccgc tgggcacccg cgagtcgagc tgggagccgg
7741 cgggcacggc gctggtcacc ggcggcaccg gtgcgctcgg cggccacgtc gcccgccacc
7801 tcgccaggtg cggggtggag gacctggtgc tggtcagcag gcgcggcgtc gacgctcccg
7861 ccgcggccga gctggaagcc gaactggtcg ccctcgggcc gaagacgacc atcaccgcct
7921 gcgacgtggc cgaccgcgag cagctctcca agctgctgga agaactgcgc gggcagggac
7981 gtccggtgcg gaccgtcgtg cacaccgccg gggtgcccga atcgaggccg ctgcacgaga
8041 tcggcgagct ggagtcggtc tgcgcggcga aggtgaccgg ggcccggctg ctcgacgagc
8101 tgtgcccgga cgccgagacc ttcgtcctgt tctcgtccgg agcgggggtg tggggcagtg
8161 cgaacctcgg cgcctactcc gcggccaacg cctacctcga cgcgctggcc caccgcgccg
8221 tgcggaaggc cgtgcgccgc acgtccgtcg cgtggggcgc ctgggcgggc gagggcatgg
8281 ccaccggcga cctcgagggg ctcacccggc gcggcctgcg cccgatggcg cccgaccgcg
8341 cgatccgcgc gctgcaccag gcgctggaca acggcgacac gtgcgtttcg atcgccgacg
8401 tcgactggga ggccttcgcg gtcggcttca ccgccgcccg gccgcgtccg ctgctggacg
8461 agctcgtcac gccggcggtg ggggccgtcc ccgcggtgca ggcggccccg gcgcgggaga
8521 tgacgtcgca ggagttgctg gagttcacgc actcgcacgt cgcggcgatc ctcgggcatt
8581 ccagcccgga cgcggtcggg caggaccagc cgttcaccga gctcggcttc gactcgctga
8641 ccgcggtcgg gctgcgcaac cagctccagc aggccaccgg gctcgcgctg cccgcgaccc
8701 tggtgttcga gcaccccacg gtccgcaggt tggccgacca cataggacag cagctcgaca
8761 gcgggactcc cgcccgggaa gcgagcagcg ctcttcgcga cggctaccgg caggcgggcg
8821 tgtcgggcag ggtccggtcc tacctcgacc tgctggcggg gctgtcggac ttccgcgagc
8881 acttcgacgg ctccgacggg ttctccctcg atctcgtgga catggccgac ggtcccggag
8941 aggtcacggt gatctgctgc gcgggaacgg cggcgatctc cggtccgcac gagttcaccc
9001 ggctcgccgg ggcgctgcgc ggaatcgctc cggttcgggc cgtgccccag cccggctacg
9061 aggagggcga acctctgccg tcgtcgatgg cggcggtggc ggcggtgcag gccgatgcgg
9121 tcatcaggac acagggggac aagccgttcg tggtggccgg tcactccgcg ggggcactga
9181 tggcctacgc gctggcgacc gaactgctcg atcgcgggca cccgccacgc ggtgtcgtcc
9241 tgatcgacgt ctacccgccc ggtcaccagg acgcgatgaa cgcctggctg gaggagctga
9301 ccgccacgct gttcgaccgc gagacggtgc ggatggacga caccaggctc accgccctgg
9361 gcgcctacga ccgcctcacc ggtcagtggc gaccccggga aaccgggctg ccgacgctgc
9421 tggtcagcgc cggcgagccg atgggtccgt ggcccgacga cagctggaag ccgacgtggc
9481 ccttcgagca cgacaccgtc gccgtccccg gcgaccactt cacgatggtg caggaacacg
9541 ccgacgcgat cgcgcggcac atcgacgcct ggctgggcgg agggaattca tgaccacgac
9601 cgatcgcgcc gggctgggca ggcagctcca gatgatccgc ggcctgcact ggggttacgg
9661 cagcaacggc gacccttacc cgatgctgct gtgcggacac gacgacgacc cgcagcgccg
9721 gtaccgctcg atgcgcgagt ccggtgtgcg gcgcagcccg accgagacg
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