GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS STMAPHF 819 bp ds-DNA BCT 27-MAY-1991
DEFINITION Streptomyces griseus aphE gene for streptomycin phosphotransferase
ACCESSION X53527
KEYWORDS aminoglycoside phosphotransferase; aphE gene; phosphotransferase;
streptomycin resistance.
SOURCE Streptomyces griseus DNA.
ORGANISM Streptomyces griseus
Prokaryota; Bacteria; Firmicutes; Streptomycetaceae.
REFERENCE 1 (bases 1 to 819)
AUTHORS Trower,M.K.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 819)
AUTHORS Trower,M.K. and Clark,K.G.
TITLE PCR cloning of a streptomycin phosphotransferase (aphE) gene from
Streptomyces griseus ATCC 12475.
JOURNAL Nucleic Acids Res. 18, 4615-4615 (1990)
STANDARD full automatic
COMMENT *source: strain=ATCC 12475;
Data kindly reviewed (06-SEP-1990) by Trower M.K.
From EMBL entry SGAPHE; dated 01-OCT-1990.
FEATURES Location/Qualifiers
CDS 1..816
/note="phosphotransferase (AA 1-272)"
/codon_start=1
BASE COUNT 85 a 300 c 310 g 124 t
ORIGIN
1 atgagtgatc accccgggcc gggggccgtc acgccggagc tgttcggcgt gggcggcgac
61 tggctggccg tcaccgcggg cgaatcgggc gcctccgtct ttcgcgccgc ggacgccacc
121 cggtacgcca agtgcgtgcc cgccgcggac gcggccggtc ttgaggcgga acgcgaccgg
181 atcgcctggc tgagcgggca gggcgtaccg ggcccccgcg tcctcgactg gtacgccggt
241 gacgcgggcg cctgcctggt cacccgtgcc gtccccggcg tacccgctga tcgggtgggc
301 gccgatgacc ttcgcactgc ctggggggcc gtcgcggacg cggtccgtcg gctgcacgag
361 gtgcccgtgg cctcgtgtcc gttccgccgg gggctggact ccgtggtcga cgccgcccgt
421 gacgtggtgg cccgtggcgc ggtgcatccg gagttcctgc cggtggagca gcggctcgtt
481 cccccggcgg agctgctggc ccggctcacc ggggagctcg cccgtcggcg cgatcaggag
541 gccgccgaca cggtcgtctg ccacggtgat ctctgcctgc ccaacatcgt cctccatccg
601 gagaccctgg aggtgtcggg cttcatcgac ctgggacggc tcggggcggc cgaccgccac
661 gccgacctgg cgctgctgct ggccaacgcg cgcgagacct gggtggacga ggagcgggcg
721 cggttcgccg acgcggcgtt cgccgagcgt tacggcatcg ccccggaccc ggaacggctg
781 cgcttctacc tccatctcga tccgctcacc tggggctag
//