GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS SMAPROBAG 2926 bp ds-DNA BCT 27-MAY-1991
DEFINITION S.marcescens proBA genes for gamma-glutamyl kinase (EC 2.7.2.11)
and gamma-glutamyl-phosphate reductase (EC 1.2.1.41)
ACCESSION X53086
KEYWORDS gamma-glutamyl kinase; gamma-glutamyl-phosphate reductase;
glutamate 5-kinase; operon; proBA genes; proBA operon.
SOURCE Serratia marcescens DNA.
ORGANISM Serratia marcescens
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2926)
AUTHORS Omori,K.
JOURNAL Unpublished (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17856; PROB$SERMA. SWISS-PROT; P17857; PROA$SERMA.
*source: strain=Sr41; isolate=8000 (wild type strain) *source:
clone=pWP102.
Data kindly reviewed (14-AUG-1990) by Omori K.
From EMBL entry SMPROBAG; dated 02-OCT-1990.
FEATURES Location/Qualifiers
CDS <1..62
/note="ORF 15kDa protein (20 AA) (1 is 2nd base in codon)"
/codon_start=1
repeat_unit 95..105
/note="inverted repeat A"
promoter 108..113
/note="put. -35 region"
repeat_unit complement(110..120)
/note="inverted repeat A'"
promoter 131..136
/note="put. -10 region"
precursor_RNA 142..2598
/note="proBA primary transcript"
misc_feature 142..144
/note="transcription start site"
RBS 173..176
/note="ribosome binding site"
CDS 182..1282
/note="gamma-glutamyl kinase (AA 1 - 367)"
/codon_start=182
RBS 1285..1288
/note="ribosome binding site"
CDS 1295..2545
/note="gamma-glutamyl phosphate reductase (AA 1 - 417)"
/codon_start=1295
terminator 2573..2598
/note="potential transcription terminator"
BASE COUNT 575 a 911 c 937 g 503 t
ORIGIN
1 ccatggaact ggggctggaa cccgccgacg atttcaaaga aaaactgatc aaactctccg
61 cctgaccccg tttgaccacg tattgtgctg atttcgcgtt gtgcctgttg gcataacgcg
121 tctctcctgt tacaaagacc tttgcactct tcttctcatc attacacacg gcagaaatac
181 tatgaacggc agccagacat tggttgtgaa attgggcacc agcgtgttga ccggcggatc
241 gctgcgtctg aaccgcgccc acatcgtcga attggtgcgc cagtgcgcgc agcaacacgc
301 cgcgggccac cgcattgtca tcgtcacctc cggcgccatc gccgccgggc gcgaacacct
361 gggctacccc gagctgcccg ccaccatcgc ttccaagcag ctgctggcag cggtggggca
421 gagccggctg atccagctgt gggaacagct gttctccatt tacggtatcc acgtcgggca
481 gatgctgctg acgcgcgccg atctggaaga ccgcgagcgc ttcctcaacg cgcgcgatac
541 catgacggcg ctgctggaca accgcatcgt gccggtaatc aatgagaacg acgccgtcgc
601 caccgccgaa atcaaggtgg gcgacaacga taacctgtcg gcactggcgg ccattctggc
661 cggggccgac aaactgctgc tgctgaccga tcagcagggc ctctacaccg ccgatccgcg
721 caacaatccg caggccgaac tgatccgtga agtgcacggc atcgacgacg cgctgcgcgc
781 catcgccggc gacagcgtct ccggtctggg caccggcggc atgggcacca agctgcaggc
841 ggccgacgtg gcctgccgcg ccggcatcga cgtggtgatc gccgcgggca gcaagccggg
901 cgtggtggcg gacgtgatcg aaggcaaacc ggtgggcacc cgtttccacg cgttggaaac
961 cccgctggaa aaccgcaagc gctggatctt tggcgcgcca ccggccggtg agatcaccgt
1021 cgacgacggc gcggtggaag cgatgatggc gcgcggcagc tcgctgttgc caaagggcat
1081 ccgcgaagtg aagggcgact tctcgcgcgg cgaagtgatc cgtatccgca acctgacggg
1141 gcgcgatctg gcgcacggcg tcagccgcta caacagcgac gcgatgcgca tgatcgccgg
1201 gcaccactcg caggaaatca gcgagatcct cggttatgaa tacggtccgg tggcagtgca
1261 ccgcgacgat atgattgtca gttaaggagt gagcatgctg gagcagatgg ggaaggccgc
1321 caagcaggcc tcctggcagc tggcggtgct gagcacggcg aagaagaatc aggtgctgtc
1381 ggtgatggcc gacaggctgg aagccaacag tgaagcgatc ctgctggcga atgaacaaga
1441 catggcgcag gcgcgcgcca ccggcatgag cgaagcgctg ctcgatcgcc tgttgctgac
1501 cccggcgcgg ctggcggcga tcgccaacga cgtgcgccag gtgtgccgcc tgaacgatcc
1561 ggtcggccac gtgctggacg gcaacctgct ggacagcggg ctgaagctgg agcgccggcg
1621 ggtgccgctc ggcgtgatcg gcgtgattta cgaggcgcgg ccgaacgtca ccatcgacgt
1681 cgccagcctg tgcctgaaaa ccggcaacgc agtgatcctg cgcggcggta aagagacgca
1741 caacaccaac caggcgacgg tgaaagtgat ccagcaggcg ttggaacagt gtggcctgcc
1801 ggcggcggcg gtgcaggcga tcgacagccc ggatcgcgcg ctggtgaacg aactgctgcg
1861 cctggatcgc tacgtcgaca tgctgatccc gcgcggcggc gccggcctac acaagctgtg
1921 ccgcgagcaa tcgaccatcc cggtgatcac cggcggcatc ggcgtgtgcc atacctatgt
1981 ggatgccgac gttgatttcg acaaggcgct gacggtgatt gaaaacgcca agatccagcg
2041 cccgagcgcc tgtaactcgc tggagacgct gctggtgaac cgcagcatcg ccgccgagtt
2101 cctgccggcg ctgagcgcca aaatggcggc ggcgggcgtg acgctgcacg cggcggaaaa
2161 cgcgctgccg ctgctgcagg gcggcccggc gacggtggtg ccggtgaacg cggaggatta
2221 cgacgacgaa tggctgtcgc tggatctgaa cgtgctgctg gtggacgata tcgatcaggc
2281 catcgatcac atccgcaccc acggcactaa ccattccgac gcgatcctca cccgttcgct
2341 cagcagcgcc gagcacttcg tgcgcgcggt ggactcctcg gcggtgtacg tcaacgccag
2401 cacccgcttc accgacggcg gccagttcgg cttgggcgct gaagtggcgg tgagcaccca
2461 gaaactgcac gcccgcggcc cgatgggcct ggacgcgctg accacctaca agtggattgg
2521 ctacggcgac gatctggtgc gtagctaagg ccgcattacg gtcataaaga ggtcagggcg
2581 caccatgctg cgccctgatt ttttttgcag cgtagctgca gtgcggctgt ttgcaaaaca
2641 accaaacgcc ccggcggcta ttgactcatt tcgcgcgcag gtctaccttg ttcaacgcac
2701 ttccggcttg ccccgcaggc ccggctcgat gccgatatag ctcagttggt agagcagcgc
2761 attcgtaatg cgaaggtcgt aggttcgact cctattatcg gcaccatttc aagtttcact
2821 gccttcctta gtaaaaatac gcagcactgg cgtctgcaac gcttgcggtt gatatttctg
2881 cttatcgcta tgtggcaata tgctgcaagg tataatacaa acgcgt
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