GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS STRAMILOC 6911 bp ds-DNA BCT 27-MAY-1991
DEFINITION Streptococcus pneumoniae ami locus conferring aminopterin
resistance
ACCESSION X17337 K00102 V01353
KEYWORDS ami locus; amiA gene; amiB gene; amiC gene; amiD gene; amiE gene;
amiF gene; aminopterin resistance; drug resistance.
SOURCE Streptococcus pneumoniae DNA.
ORGANISM Streptococcus pneumoniae
Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
Streptococcaceae.
REFERENCE 1 (sites)
AUTHORS Claverys,J.P., Mejean,V., Gasc,A.M., Galibert,F. and Sicard,A.M.
TITLE Base specificity of mismatch repair in Streptococcus pneumoniae
JOURNAL Nucleic Acids Res. 9, 2267-2280 (1981)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Claverys,J.P., Mejean,V., Gasc,A.M. and Sicard,A.M.
TITLE Mismatch repair in Streptococcus pneumoniae: relationship between
base mismatches and transformation efficiencies
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80, 5956-5960 (1983)
STANDARD full staff_review
REFERENCE 3 (bases 1 to 6911)
AUTHORS Claverys,J.P.
JOURNAL Unpublished (1989)
STANDARD full automatic
REFERENCE 4 (bases 1 to 6911)
AUTHORS Alloing,G., Trombe,M.C. and Claverys,J.P.
TITLE The ami locus of the Gram-positive bacterium Streptococcus
pneumoniae is similar to binding protein-dependent transport
operons of Gram-negative bacteria.
JOURNAL Mol. Microbiol. 4, 633-644 (1990)
STANDARD full automatic
COMMENT *source: strain=R800;
Data kindly reviewed (14-SEP-1990) by Claverys J.P.
From EMBL entry SPAMILOC; dated 24-SEP-1990.
FEATURES Location/Qualifiers
promoter 210..215
/note="-10 region"
CDS 256..1734
/note="AmiA protein (AA 1-493)"
/codon_start=256
CDS 1746..2234
/note="AmiB protein (AA 1-163)"
/codon_start=1746
CDS 2304..3797
/note="AmiC protein (AA 1-498)"
/codon_start=2304
CDS 3800..4723
/note="AmiD protein (AA 1-308)"
/codon_start=3800
CDS 4735..5799
/note="AmiE protein (AA 1-355)"
/codon_start=4735
CDS 5813..6736
/note="AmiF protein (AA 1-308)"
/codon_start=5813
BASE COUNT 2053 a 1298 c 1427 g 2133 t
ORIGIN
1 gaattccaaa tcacattgtt ccgtccgtat ttcattttac tgtatgtctt cctaaactcc
61 aaagtttctg cgtcaaattt cctattttca ctgtagtctt tgacacttcg aacgacaatt
121 tgaatcatat attcaaaaaa aaatgaaaaa tttgtgccaa caagcctaaa tattctgaaa
181 atttgtttga tttttaatgg ataatgtgat ataatggttg tcaatctata attcacataa
241 acagaggagt agaaaatgaa aaaaaatcgt gtatttgcta cagcaggtct tgttttatta
301 gcagcaggtg tacttgcagc atgcagttct tcaaaatcat ctgattcatc agcccctaaa
361 gcttatggct atgtttatac agcagaccca gaaaccttgg actacctgat ttcacgtaaa
421 aatagtacaa cagtagtgac ttcaaatggg attgatggtt tattcactaa cgataattac
481 ggtaatcttg ctcctgcagt tgcagaggat tgggaagtct ctaaggatgg tttgacctac
541 acttataaga ttcgtaaagg ggttaaatgg tttacctctg atggagaaga atatgcagag
601 gtgacggcta aagatttcgt gaacggttta aaacacgcag cagataaaaa atcagaagct
661 atgtatttag ctgaaaattc ggttaaaggc ttggcagatt atctatcagg aacttcaaca
721 gatttttcaa cagttggtgt caaggcggtt gatgattata cgttacaata cactttgaac
781 cagcctgaac cgttctggaa ctctaagttg acctattcta ttttctggcc tctgaatgaa
841 gaattcgaaa catcaaaagg aagcgatttt gctaaaccaa cagatccgac atccttgctt
901 tataatggtc cattcttgtt gaaagggttg actgcaaaat cttctgtaga gtttgtaaaa
961 aatgagcaat attgggataa agaaaatgtc cacctagata ctatcaatct agcttactat
1021 gatggatcag atcaggagtc gctagagcgt aacttcacta gtggagctta tagttatgcc
1081 cgtctttacc ctaccagctc caactattct aaggttgcag aagaatacaa ggacaatatc
1141 tattacacac aatcaggctc tgggattgct ggtctgggtg tgaatattga tcgccaaagt
1201 tacaactata cttctaaaac tacagattca gagaaagtag ctactaagaa ggcattgctt
1261 aacaaagatt tccgtcaagc cttgaatttt gctcttgatc gctcagctta ctcagctcaa
1321 atcaatggta aagatggagc agctttagca gttcgtaatt tatttgtaaa accagacttt
1381 gtttcagctg gtgagaagac ctttggtgat ttagtcgctg ctcaacttcc tgcttatggt
1441 gatgagtgga aaggtgtgaa tttagctgat gggcaggatg gtttattcaa tgctgacaag
1501 gccaaggcag agtttcgcaa agctaagaaa gctttagaag cagacggcgt tcagtttcct
1561 attcatctgg acgttccagt agaccaagca tcaaaaaact acatatctcg tattcagtcc
1621 tttaaacaat ctgtagaaac agttcttggt gttgaaaatg tcgttgttga tattcaacaa
1681 atgacaagtg atgaattcct taatattact tactatgact gccaatgctt catctgagga
1741 ttgggatgta tcaggaggag ttatcatggg ggccagacta tcaagaccca tctacttacc
1801 tggatatttt aaaaacaact agcagtgaaa ctacaaaaac atatttagga tttgataatc
1861 caaatagccc ttcagtagtt caagttggtt tgaaagaata cgataaatta gttgatgaag
1921 ctgccaaaga gacaagcgac ttcaatgtcc gttatgaaaa atatgcagcg gctcaagcat
1981 ggttgacaga tagttcactc tttattcctg ctatggcttc ttctggtgca gcaccagtgc
2041 tttcacgaat tgttccattt actggagctt ctgcgcaaac aggctctaag gggtcagatg
2101 tttacttcaa atatttgaaa ttacaagata aagcggtgac taaggaagag tatgaaaaag
2161 ctcgtgaaaa atggttgaaa gaaaaagctg aatcaaatga gaaagctcaa aaagaattgg
2221 caagtcatgt gaagtaaata atttctaata caactttctc tccatgagga gaaggttctt
2281 ttgggatttt taaaggaaat aatatgaaaa aatatatttt tatgcgtgtt ttgcggtcat
2341 tggtttcgat tttcttagta acgactttga cctacacgat tatctatacc ttggttcctc
2401 gaaaattgat tttcaagcag gatcctaact ataataaaat tgcgacaacg gctgataaac
2461 gtgataacta tgaaaatact gtgtttgagc gtatgggcta cattgagtat tacgatacta
2521 aagagttgca agaaaaggca agtagcatgg attcttctgt aacagtagaa gcaaatgcga
2581 ccaataaagc tatttatgaa aagtacatca atcaattagg tcatggttgg actttgggag
2641 aatttactga aagtggtcaa ttctatgcta ctcgtgaaat tccaattttt gaacgtgttt
2701 ttcacttcta tgctaacttg attgacattg accatacaaa taaaatccaa gaccctgaaa
2761 atccagactt gaaacgctac cttcgttttg aaaatgatcc agctatcgga tggtcattgg
2821 tcggttcagg aactaaacat aaatatctct tgtactttaa cagtcagttc ccatttgttc
2881 atcaaaactt tgtgaacttg aatttaggtg actcttaccc aacctatgct aatacaccag
2941 ttcttcaggt tattactcaa ggtcaaggac aaaccaaaac tgcccaagtt cagttcccaa
3001 caggtaagaa aacgtcttct gtaaatattt actcaagaac ctacaagtca cctagtcagg
3061 ctgactctcg tgaagtagct agctatggga aagatgatcc ttatacagcg actgaaagta
3121 attaccaata tccatctatg attgtcagct ctgctattac tggtttgatt ggtttggttc
3181 ttgcctatgc tcttgccgtg ccacttggtt cagccatggc tcgtttcaag aacacttgga
3241 ttgatagcct ctcaacaggg gctttgacct tcttgcttgc tcttccaacg attgccttgg
3301 tttacatcgt tcgattgatt ggatcatcta ttgcccttcc agattcattc cctatcttgg
3361 gagctggaga ttggcgttct tacgttttac cagcagtcat ccttggtttg ttgggtgctc
3421 ctggtacagc catttggatt cgtcgttaca tgattgactt gcaatctcaa gactttgttc
3481 gtttcgctcg tgcaaaaggt ttgtctgaaa aagaaatttc aaacaaacac atctttaaaa
3541 atgccatggt tccgctggtt tcaggaattc ctgctgccat tattggggtt atcggtggtg
3601 caacccttac tgaaacagtc ttcgccttcc caggtatggg taaaatgttg attgactctg
3661 taaaagcatc taataactct atggtcgttg gtcttgtctt catctttaca tgtatttcta
3721 tcttctcacg tcttttggga gatatttgga tgactattat tgacccacgt attaaattga
3781 ctgagaaagg aggcaaataa tgtctacaat cgataaagaa aaatttcagt ttgtaaaacg
3841 tgacgatttt gcctctgaaa ctattgatgc gccagcatat tcttactgga aatcagtgtt
3901 taaacaattt atgaagaaaa aatcaactgt agtcatgttg ggaatcttgg tagccatcat
3961 tttgataagt ttcatctacc caatgttttc taagtttgat ttcaatgatg tcagcaaggt
4021 aaacgacttt agtgttcgtt atatcaagcc aaatgcggag cattggttcg gtactgacag
4081 taacggtaaa tcgctctttg acggtgtctg gttcggagct cgtaactcca tcctcatttc
4141 tgtgattgcg acagtgatta acttggttat cggtgttttt gtcggtggta tttggggtat
4201 ttcaaaatca gttgaccgtg tcatgatgga agtttacaac gtcatctcaa acatcccacc
4261 tcttttgatt gttattgtct tgacttactc aatcggagct ggattctgga atctgatttt
4321 tgccatgagc gtaacaacat ggattggtat tgccttcatg atccgtgtgc aaatcttgcg
4381 ctatcgtgac ttggaataca acttggcgtc acgtactttg ggaacaccaa ccttgaagat
4441 tgttgccaaa aatatcatgc ctcaattggt atctgttatt gtgacaacca tgactcaaat
4501 gcttccaagc tttatctcat acgaagcctt cttgtctttc ttcggtcttg gattaccgat
4561 tacagtgcca agtttgggtc gtttgatttc ggattattca caaaacgtaa caaccaatgc
4621 ttacttgttc tggattccat tgacaaccct tgtcttggta tccttgtccc ttttcgtagt
4681 tggtcaaaac ttagcggatg ctagtgatcc acgtacacat agataggagt agaaatgaca
4741 aaagaaaaaa atgtaatttt gactgctcgc gatattgtcg tggaatttga cgttcgtgac
4801 aaagtattga cagccattcg cggcgtttcc cttgaactag tcgaaggaga agtattagcc
4861 ttggtaggtg agtcaggatc aggtaaatct gttttgacaa agaccttcag aggtatgctc
4921 gaagaaaatg gtcgtattgc cgaaggtagt attgactacc gtggtcagga cttgacagct
4981 ttatcttctc acaaggattg ggaacaaatt cgtggtgcta agattgcgac tatcttccaa
5041 gacccaatga ctagtttgga ccccattaaa acaattggta gtcagattac agaagttatt
5101 gtaaaacacc aaggaaaaac agctaaagaa gcgaaagaat tggccattga ctacatgaat
5161 aaggttggca ttccagacgc agatagacgt tttaatgaat acccattcca atattctgga
5221 ggaatgcgtc aacgtatcgt tattgcgatt gcccttgcct gccgacctga tgtcttgatc
5281 tgtgatgagc caacaactgc cttggatgta actattcaag ctcagattat tgatttgcta
5341 aaatctttac aaaacgagta tcatttcaca acaatcttta ttacccacga ccttggtgtg
5401 gtggcaagta ttgcggataa ggtagcggtt atgtatgcag gagaaatcgt tgagtatgga
5461 acggttgagg aagtcttcta tgaccctcgc catccatata catggagtct cttgtctagc
5521 ttgcctcagc ttgctgatga taaaggggat ctttactcaa tcccaggaac acctccgtca
5581 ctttatactg acctgaaagg ggatgctttt gccttgcgtt ctgactacgc aatgcagatt
5641 gacttcgaac aaaaagctcc tcaattctca gtatcagaga cacattgggc taaaacttgg
5701 cttcttcatg aggatgctcc aaaagtagaa aaaccagctg tgattgcaaa tctccatgat
5761 aagatccgtg aaaaaatggg atttgcccat ctggctgact aggaggaagg aaatgtctga
5821 aaaattagta gaaatcaaag atttagaaat ttccttcggt gaaggaagta agaagtttgt
5881 cgcggttaaa aatgctaact tctttatcaa caagggagaa actttctcgc ttgtaggtga
5941 gtccggtagt gggaaaacaa ctattggtcg tgctatcatc ggtctaaatg atacaagtaa
6001 tggagatatc atttttgatg gtcaaaagat taatggtaag aaatcgcgtg aacaagctgc
6061 ggaattgatt cgtcgaatcc agatgatttt ccaagaccct gccgcaagtt tgaatgaacg
6121 tgcgactgtt gattatatta tttctgaagg tctttacaat caccgtttat ttaaggatga
6181 agaagaacgt aaagagaaag ttcaaagtat tatccgtgaa gtaggtcttc ttgctgagca
6241 cttgactcgt taccctcatg aattctcagg cggtcaacgt caacgtatcg gtattgcccg
6301 tgccttggtc atgcaaccag actttgttat tgcagatgag ccaatttcag ccttggacgt
6361 ttctgtacgt gcccaagtct tgaacttgct caaaaaattc caaaaagagc tcggcttgac
6421 ctatctcttc atcgcccatg acttgtcggt tgttcgcttt atttcagatc gtatcgcagt
6481 tatttacaag ggtgttattg tagaggttgc agaaacagaa gaattgttta acaatccaat
6541 tcacccatat actcaagcct tgctttcagc ggtaccaatc ccagatccaa tcttggaacg
6601 taagaaggtc ttgaaggttt acgagggaag tcaacacgac tatgagactg ataagccatc
6661 tatggtagaa atccgtccag gtcactatgt ttgggcgaac caagccgaat tagcacgtta
6721 tcaaaaagga ctaaactaat aatggtttta taatttccat gtcaattttt atgggaatta
6781 ggctctttgt caactgtagt gggttgaagt cagctaagct cgagaaagga caaatttcgt
6841 cctttctttt ttgatgttca aagcgataaa aatccgtttt ttgaagtttt caaagttccg
6901 aaaaccaaag g
//