GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS STRPLY 1650 bp ds-DNA BCT 27-MAY-1991
DEFINITION S.pneumoniae ply gene for pneumolysin
ACCESSION X52474
KEYWORDS hemolysin; ply gene; pneumolysin; sulfhydryl-activated toxin.
SOURCE Streptococcus pneumoniae DNA.
ORGANISM Streptococcus pneumoniae
Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
Streptococcaceae.
REFERENCE 1 (bases 1 to 1650)
AUTHORS Walker,J.A., Allen,R.L., Falmagne,P., Johnson,M.K. and
Boulnois,G.J.
TITLE Molecular cloning, characterisation and complete nucleotide
sequence of the gene for pneumolysin, the sulphydryl-activated
toxin of streptococcus pneumoniae.
JOURNAL Infect. Immun. 55, 1184-1189 (1987)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1650)
AUTHORS Mitchell,T.J.
JOURNAL Unpublished (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P11990; PNEU$STRPN.
*source: strain=NCTC 7466, serotype II;
Data kindly reviewed (20-JUL-1990) by Mitchell T.J.
From EMBL entry SPPLY; dated 25-SEP-1990.
FEATURES Location/Qualifiers
promoter 15..20
/note="promoter"
promoter 38..43
/note="promoter"
RBS 97..101
/note="ribosome binding site"
CDS 208..1620
/note="pneumolysin (AA 1-471)"
/codon_start=208
BASE COUNT 499 a 290 c 401 g 460 t
ORIGIN
1 ttacaagacc aaccttgatt gacttagata aggtatttat gttggataat acggttattc
61 cgacttctta tctagccaga cggcgacgca atgtctcaga agaattgtac gaggaaattt
121 tggatcactt agtccaacca cggctgattt cgctgaacaa gtctgagttt atgcaactca
181 atccaggaac ttattaggag gtagaagatg gcaaataaag cagtaaatga ctttatacta
241 gctatgaatt acgataaaaa gaaactcttg acccatcagg gagaaagtat tgaaaatcgt
301 ttcatcaaag agggtaatca gctacccgat gagtttgttg ttatcgaaag aaagaagcgg
361 agcttgtcga caaatacaag tgatatttct gtaacagcta ccaacgacag tcgcctctat
421 cctggagcac ttctcgtagt ggatgagacc ttgttagaga ataatcccac tcttcttgcg
481 gttgatcgtg ctccgatgac ttatagtatt gatttgcctg gtttggcaag tagcgatagc
541 tttctccaag tggaagaccc cagcaattca agtgttcgcg gagcggtaaa cgatttgttg
601 gctaagtggc atcaagatta tggtcaggtc aataatgtcc cagctagaat gcagtatgaa
661 aaaataacgg ctcacagcat ggaacaactc aaggtcaagt ttggttctga ctttgaaaag
721 acagggaatt ctcttgatat tgattttaac tctgtccatt caggtgaaaa gcagattcag
781 attgttaatt ttaagcagat ttattataca gtcagcgtag acgctgttaa aaatccagga
841 gatgtgtttc aagatactgt aacggtagag gatttaaaac agagaggaat ttctgcagag
901 cgtcctttgg tctatatttc gagtgttgct tatgggcgcc aagtctatct caagttggaa
961 accacgagta agagtgatga agtagaggct gcttttgaag ctttgataaa aggagtcaag
1021 gtagctcctc agacagagtg gaagcagatt ttggacaata cagaagtgaa ggcggttatt
1081 ttagggggcg acccaagttc gggtgcccga gttgtaacag gcaaggtgga tatggtagag
1141 gacttgattc aagaaggcag tcgctttaca gcagatcatc caggcttgcc gatttcctat
1201 acaacttctt ttttacgtga caatgtagtt gcgacctttc aaaacagtac agactatgtt
1261 gagactaagg ttacagctta cagaaacgga gatttactgc tggatcatag tggtgcctat
1321 gttgcccaat attatattac ttgggatgaa ttatcctatg atcatcaagg taaggaagtc
1381 ttgactccta aggcttggga cagaaatggg caggatttga cggctcactt taccactagt
1441 attcctttaa aagggaatgt tcgtaatctc tctgtcaaaa ttagagagtg taccgggctt
1501 gcctgggaat ggtggcgtac ggtttatgaa aaaaccgatt tgccactagt gcgtaagcgg
1561 acgatttcta tttggggaac aactctctat cctcaggtag aggataaggt agaaaatgac
1621 taggagagga gaatgcttgc gacaaaaaga
//