GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS STYCARAB 1886 bp ds-DNA BCT 27-MAY-1991
DEFINITION Salmonella carA gene and carB gene (partial) for
carbamoyl-phosphate synthetase (EC 6.3.5.5)
ACCESSION X13200
KEYWORDS carA gene; carB gene; carbamoyl-phosphate synthetase.
SOURCE Salmonella typhimurium DNA.
ORGANISM Salmonella typhimurium
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1886)
AUTHORS Kilstrup,M., Lu,C.-D., Abdelal,A. and Neuhard,J.
TITLE Nucleotide sequence of the carA gene and regulation of the carAB
operon in Salmonella typhimurium
JOURNAL Eur. J. Biochem. 176, 421-429 (1988)
STANDARD full automatic
COMMENT SWISS-PROT; P14845; CARA$SALTY. SWISS-PROT; P14846; CARB$SALTY.
*source: strain=LT2;
From EMBL entry STCARAB; dated 30-AUG-1990.
FEATURES Location/Qualifiers
repeat_region 232..249
/note="direct repeat"
repeat_region 264..283
/note="direct repeat"
repeat_region 379..396
/note="direct repeat"
repeat_region complement(531..548)
/note="direct repeat"
promoter 560..565
/note="pot. -35 region"
promoter 584..589
/note="pot. -10 region"
precursor_RNA 596..596
/note="pot. transcription start site"
promoter 630..635
/note="pot. alt. -35 region"
misc_feature 649..664
/note="arginine repressor binding site"
promoter 654..659
/note="pot. alt. -10 region"
precursor_RNA 666..666
/note="pot. alt. transcription start site"
misc_feature 668..685
/note="arginine repressor binding site"
RBS 686..690
/note="pot. ribosome binding site"
CDS 696..1841
/note="carbamoyl-phosphate synthetase (AA 1 - 382)"
/codon_start=696
misc_feature 1500..1502
/note="active site Cys residue"
misc_feature 1752..1754
/note="active site His residue"
RBS 1848..1851
/note="pot. ribosome binding site"
CDS 1863..>1886
/note="carB gene product (AA 1 - 8)"
/codon_start=1863
BASE COUNT 463 a 437 c 458 g 528 t
ORIGIN
1 agcgccttac ctatgcaaaa ggcacctcga ccagactgat aagactgata tccatcggag
61 ggggagtgct gttcctcggg ggggcgtttt atctcttttg cttcaatagg ttggcttttt
121 tatgctgtag tgaggctggt tgtggtccaa tcgtatgtgc ctgtcttaaa gaaaaagaag
181 ctggatcttt atatcgcgat tgtaattctt attacattgt cttaatttgc tgttttttgt
241 tgattttatg atgcatttga tttgtttttt atatttttaa tatattgatt tttaaaattt
301 ttttgtcgtt aactttcttg attttgaatt tttatgcatt taatggcatt gatggctatt
361 aagtgagttt ttatcgttgt tttttgttgt tttaatgtca aatttgacca tttggtccac
421 tttttatcat gccagccagt tttttgcgaa ctcaaggaag cgcaagcgtt ttctatcgta
481 acttagttga tctttttgcc tcttaaacag aataacttcc ttataatgtg caaaataaca
541 taaaaaacac cctctttatg ttgactttta tccggcttaa cttcagaatg ccgccgtttg
601 ccagaaatcc acgggtaagc aaatttgcat tgcttcatac tgactgaatg aattaatatg
661 caaataaagt gagtgaatat tctctggagg gtgttttgat taagtcagcg ctattggttc
721 tggaagacgg aacccatttt cacggtcggg ccattggggc aacgggttcg gcggttgggg
781 aagtcgtttt caatacttca atgaccggtt atcaagaaat cgtcactgat ccttcctatt
841 cccgccaaat cgtcactctt acttatcccc atattggtaa tgtcggcacc aataaagccg
901 atgaagaatc ctctcaggtt catgcgcaag gtttagtgat tcgcgatctg ccgttgattg
961 ccagcaactt ccgtaacacc gaagacctct cttcttacct caaacggcat aacattgtgg
1021 cgattgctga tatcgatacc cgcaagctga cccgactgct gcgtgagaaa ggcgcacaga
1081 acggctgcat cattgcaggc gatagcccgg atgcgaagct ggcgctggaa aaagcgaaag
1141 catttcctgg acttaacggt atggatctgg cgaaagaagt gaccacggcg gaaacttacc
1201 gctggacgca gggtagctgg acgctgaaag acggtttacc ggaagcgaaa tctgaggatg
1261 acctgccgtt ccatgtggtg gcctatgatt tcggcgcgaa gcgcaacatc ctgcgtatgc
1321 tggtggatcg cggctgccgc ctgacggtgg tgccagcgca aacctctgct gaagaggtgc
1381 tgaaaatgaa tccggacggc atcttcctgt caaacggccc tggcgacccg gctccgtgcg
1441 actacgcgat tactgctatc cagaaattcc tcgaaaccga cattccgcta tttggcatct
1501 gcctcggtca tcagctactg gcgctggcga gcggtgcgaa gaccgttaag atgaagttcg
1561 gccatcacgg cggcaaccat ccggtaaaag atatggacag aaacgtggta atgatcaccg
1621 cgcagaacca cggctttgcg gtggatgaag actcgctgcc cgccaacctg cgcgtgaccc
1681 acaagtcgct gttcgacggc accctgcaag ggattcaccg taccgacaaa ccggcgttca
1741 gcttccaggg ccacccggag gcgagccccg gcccgcacga tgcggcgccg ctgttcgacc
1801 actttatcga gcttattgag caataccgtc agtccgcgaa ataatcagga gtaataagag
1861 ccatgccaaa acgtacagat ataaaa
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