GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS STYLEUA 497 bp ds-DNA BCT 27-MAY-1991 DEFINITION Salmonella typhimurium leu operon control region and fragment of leuA cistron coding for alpha-isopropyl-malate synthetase ACCESSION X00059 KEYWORDS attenuator; synthetase. SOURCE Salmonella typhimurium DNA. ORGANISM Salmonella typhimurium Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 1 to 497) AUTHORS Gemmill,R.M., Jones,J.W., Haughn,G.W. and Calvo,J.M. TITLE Transcription initiation sites of the leucine operons of Salmonella typhimurium and Escherichia coli JOURNAL J. Mol. Biol. 170, 39-59 (1983) STANDARD full automatic COMMENT Data kindly reviewed (23-JUL-1985) by J.M. Calvo From EMBL entry STLEUA; dated 06-JUL-1989. FEATURES Location/Qualifiers promoter 172..201 /note="RNA polymerase binding site signal" mRNA 208..>497 /note="messenger RNA" misc_feature 208..369 /note="leader sequence" attenuator 339..364 /note="attenuation signal" RBS 397..400 /note="ribosome binding site" CDS 408..>497 /note="leuA gene alpha-isopropyl-malate-synthetase" /codon_start=408 BASE COUNT 152 a 123 c 107 g 115 t ORIGIN 1 ggccattccc ctaaccaatt tgcaaacaaa aatgcctaaa tatttcaaac aatgaaaagt 61 atgaatgtcc actcgttctt ttatccttta ttggacgcgc agcccagcac aattagctaa 121 agtacgtatc cggatatcgt caacaaaatg caatggcgac agaaaataga gttgacatta 181 aacggcatat ccagtaccac taaaagcata acgcattcgc tggagctgaa ttaatgtcac 241 atatcgttcg tttcactggg ctactactac tcaacgcatt tattgtgcgc ggtagaccgg 301 tgggcggcat tcaacattaa gtcagctcga agtcaaacaa aacccgcgcc gttgcgcggg 361 tttttttatg cctgacgcaa ggcgcccctg gagacaagga ccactacatg agccagcaag 421 tcattatttt tgatacgacc ttacgcgacg gcgaacaagc gttacaggcc agcctgagcg 481 cgaaagagaa gctgcag //