GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS STYLEUA 497 bp ds-DNA BCT 27-MAY-1991
DEFINITION Salmonella typhimurium leu operon control region and fragment of
leuA cistron coding for alpha-isopropyl-malate synthetase
ACCESSION X00059
KEYWORDS attenuator; synthetase.
SOURCE Salmonella typhimurium DNA.
ORGANISM Salmonella typhimurium
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 497)
AUTHORS Gemmill,R.M., Jones,J.W., Haughn,G.W. and Calvo,J.M.
TITLE Transcription initiation sites of the leucine operons of Salmonella
typhimurium and Escherichia coli
JOURNAL J. Mol. Biol. 170, 39-59 (1983)
STANDARD full automatic
COMMENT Data kindly reviewed (23-JUL-1985) by J.M. Calvo
From EMBL entry STLEUA; dated 06-JUL-1989.
FEATURES Location/Qualifiers
promoter 172..201
/note="RNA polymerase binding site signal"
mRNA 208..>497
/note="messenger RNA"
misc_feature 208..369
/note="leader sequence"
attenuator 339..364
/note="attenuation signal"
RBS 397..400
/note="ribosome binding site"
CDS 408..>497
/note="leuA gene alpha-isopropyl-malate-synthetase"
/codon_start=408
BASE COUNT 152 a 123 c 107 g 115 t
ORIGIN
1 ggccattccc ctaaccaatt tgcaaacaaa aatgcctaaa tatttcaaac aatgaaaagt
61 atgaatgtcc actcgttctt ttatccttta ttggacgcgc agcccagcac aattagctaa
121 agtacgtatc cggatatcgt caacaaaatg caatggcgac agaaaataga gttgacatta
181 aacggcatat ccagtaccac taaaagcata acgcattcgc tggagctgaa ttaatgtcac
241 atatcgttcg tttcactggg ctactactac tcaacgcatt tattgtgcgc ggtagaccgg
301 tgggcggcat tcaacattaa gtcagctcga agtcaaacaa aacccgcgcc gttgcgcggg
361 tttttttatg cctgacgcaa ggcgcccctg gagacaagga ccactacatg agccagcaag
421 tcattatttt tgatacgacc ttacgcgacg gcgaacaagc gttacaggcc agcctgagcg
481 cgaaagagaa gctgcag
//