GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS STYTUFA 1281 bp ds-DNA BCT 27-MAY-1991
DEFINITION S. typhimurium tufA gene for elongation factor Tu
ACCESSION X55116
KEYWORDS elongation factor; elongation factor tu; translocation; tufA gene.
SOURCE Salmonella typhimurium DNA.
ORGANISM Salmonella typhimurium
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1281)
AUTHORS Tuohy,T.M. and Atkins,J.F.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Tuohy,T.M., Thompson,S.A., Gesteland,R.F., Hughes,D. and
Atkins,J.F.
TITLE The role of EF-Tu and other translation components in determining
translocation step size
JOURNAL Biochim. Biophys. Acta 1050, 274-278 (1990)
STANDARD full staff_review
COMMENT *source: strain=LT2 trpE91 (CSHSQB 31:203-214) **map: map position=
71 mins. See <J01690> and <J01691> for related E.coli sequences.
Sequence derived by PCR from chromosomal DNA.
From EMBL entry STTUFA; dated 31-DEC-1990.
FEATURES Location/Qualifiers
RBS 59..62
mat_peptide 72..1253
/product="elongation factor Tu"
/gene="tufA"
CDS 72..1256
/product="elongation factor Tu"
/gene="tufA"
/codon_start=72
BASE COUNT 314 a 328 c 353 g 286 t
ORIGIN
1 gccgcagggt taaaaaccaa aatcccgtgc tctctcctga aggggagagc gctatagtaa
61 ggaatatagc cgtgtctaaa gaaaaatttg aacgtacaaa accgcacgtt aacgttggta
121 ctatcggcca cgttgatcac ggtaaaacaa cgctgaccgc tgccattact accgtactgg
181 ctaaaaccta cggcggtgcc gctcgcgcat tcgaccagat cgataacgcg ccggaagaaa
241 aagcgcgtgg tatcaccatc aacacttctc acgttgaata cgataccccg acccgccact
301 acgcacacgt agactgcccg gggcacgctg actatgttaa aaacatgatc accggtgctg
361 ctcagatgga cggcgcgatc ctggttgttg ctgcgactga cggcccgatg ccgcagaccc
421 gtgagcacat cctgctgggt cgtcaggtag gcgttccgta catcatcgtg ttcctgaaca
481 aatgcgacat ggttgatgac gaagagctgc tggaactggt tgagatggaa gttcgcgaac
541 tgctgtctca gtacgacttc ccgggcgacg acactccgat cgttcgtggt tctgctctga
601 aagcgctgga aggcgacgca gagtgggaag cgaaaatcat cgaactggct ggcttcctgg
661 attcttatat tccggaacca gagcgtgcga ttgacaagcc gttcctgctg ccgatcgaag
721 acgtattctc catctccggt cgtggtaccg ttgttaccgg tcgtgtagag cgcggtatca
781 tcaaagtggg cgaagaagtt gaaatcgttg gtatcaaaga gactcagaag tctacctgta
841 ctggcgttga aatgttccgc aaactgctgg acgaaggccg tgccggtgag aacgtaggtg
901 ttctgctgcg tggtatcaaa cgtgaagaaa tcgaacgtgg tcaggtactg gctaagccgg
961 gcaccatcaa gccgcacacc aagttcgaat ctgaagtgta cattctgtcc aaagatgaag
1021 gcggccgtca tactccgttc ttcaaaggct accgtccgca gttctacttc cgtactactg
1081 acgtgactgg taccatcgaa ctgccggaag gcgtagagat ggtaatgccg ggcgacaaca
1141 tcaaaatggt tgttaccctg atccacccga tcgcgatgga cgacggtctg cgtttcgcaa
1201 tccgtgaagg cggccgtacc gttggcgcgg gcgttgtagc aaaagttctg ggctaattac
1261 tcgttaatta gttttgaatt g
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