GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS STYTUFB 1308 bp ds-DNA BCT 27-MAY-1991
DEFINITION S. typhimurium tufB gene for elongation factor Tu
ACCESSION X55117
KEYWORDS elongation factor; elongation factor tu; translocation; tufb gene.
SOURCE Salmonella typhimurium DNA.
ORGANISM Salmonella typhimurium
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1308)
AUTHORS Tuohy,T.M. and Atkins,J.F.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Tuohy,T.M., Thompson,S.A., Gesteland,R.F., Hughes,D. and
Atkins,J.F.
TITLE The role of EF-Tu and other translation components in determining
translocation step size
JOURNAL Biochim. Biophys. Acta 1050, 274-278 (1990)
STANDARD full staff_review
COMMENT *source: strain=LT2 trpE91 (CSHSQB 31:203-214) **map: map position=
88 mins. See Gene 12:33-39 for related E.coli sequences. Sequence
derived by PCR from chromosomal DNA.
From EMBL entry STTUFB; dated 31-DEC-1990.
FEATURES Location/Qualifiers
misc_feature 33..37
/note="RNA polymerase binding site"
misc_feature 52..58
/note="potential RNA polymerase binding site"
RBS 78..83
mat_peptide 91..1272
/product="elongation factor Tu"
/gene="tufB"
CDS 91..1275
/product="elongation factor Tu"
/gene="tufB"
/codon_start=91
BASE COUNT 315 a 338 c 358 g 297 t
ORIGIN
1 tcttctgtta ttagcattca acaagtcggg cgtgttgcct ggttgatgtg gtgatatcac
61 cgatttatcc gtgtcttaga gggacaatcg atgtctaaag aaaagtttga acgtacaaaa
121 ccgcacgtta acgtcggtac tatcggccac gttgaccatg gtaaaacaac gctgaccgct
181 gccattacta ccgtactggc taaaacctac ggcggtgccg ctcgcgcatt cgaccagatc
241 gataacgcgc cggaagaaaa agcgcgtggt atcaccatca acacttctca cgttgaatac
301 gataccccga cccgccacta cgcacacgta gactgcccgg ggcacgccga ctatgttaaa
361 aacatgatca ccggtgctgc tcagatggac ggcgcgatcc tggttgttgc tgcgactgac
421 ggtccgatgc cgcagacccg tgagcacatc ctgctgggtc gtcaggtagg cgtcccgtac
481 atcatcgtgt tcctgaacaa atgcgacatg gttgatgacg aagagctgct ggaactggtt
541 gaaatggaag ttcgtgaact tctgtctcag tacgacttcc cgggcgacga cacgccgatc
601 gttcgtggtt ctgctctgaa agcgctggaa ggcgacgcag agtgggaagc gaaaatcatc
661 gaactggctg gcttcctgga ttcttacatc ccggaaccag agcgtgcgat tgacaagccg
721 ttcctgctgc cgatcgaaga cgtattctcc atctccggtc gtggtaccgt tgttaccggt
781 cgtgtagaac gcggtatcat caaagtgggc gaagaagttg aaatcgttgg tatcaaagag
841 actcagaagt ctacctgtac tggcgttgaa atgttccgca aactgctgga cgaaggccgt
901 gccggtgaga acgtaggtgt tctgctgcgt ggtatcaaac gtgaagaaat cgaacgtggt
961 caggtactgg ctaagccggg caccatcaag ccgcacacca agttcgaatc tgaagtgtac
1021 attctgtcca aagatgaagg cggccgtcat actccgttct tcaaaggcta ccgtccgcag
1081 ttctacttcc gtactactga cgtgactggc accatcgaac tgccggaagg cgtagagatg
1141 gtaatgccgg gcgacaacat caaaatggtt gttaccctga tccacccgat cgcgatggac
1201 gacggtctgc gtttcgcaat ccgtgaaggc ggccgtaccg ttggcgcggg cgttgttgct
1261 aaagttctcg gctaatcgct gataacattt gacgcaatgc gcgaataa
//