GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS TTHMDH 3205 bp ds-DNA BCT 27-MAY-1991
DEFINITION Thermus aquaticus sucD and mdh genes for succinyl-CoA synthetase
alpha-subunit and malate dehydrogenase (EC 1.1.1.37)
ACCESSION X56033
KEYWORDS malate dehydrogenase; mdh gene; sucD gene; succinyl-CoA synthetase.
SOURCE Thermus aquaticus DNA.
ORGANISM Thermus aquaticus
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE 1 (bases 1 to 3205)
AUTHORS Nicholls,D.J.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 3205)
AUTHORS Nicholls,D.J., Sundaram,T.K., Atkinson,T. and Minton,N.P.
TITLE Cloning and nucleotide sequences of the mdh and sucD genes from
Thermus aquaticus B
JOURNAL FEMS Microbiol. Lett. 70, 7-14 (1990)
STANDARD full automatic
COMMENT *source: strain=B
Data kindly reviewed (21-NOV-1990) by Nicaolls D.J.
From EMBL entry TAMDH; dated 28-NOV-1990.
FEATURES Location/Qualifiers
CDS <1..87
/note="ORF, pot. sucC gene"
/codon_start=1
CDS 87..950
/note="sucD product, succinyl-CoA synthetase"
/codon_start=87
CDS 968..1948
/note="mdh product, malate dehydrogenase"
/codon_start=968
terminator 1966..1994
/note="transcription terminator"
BASE COUNT 569 a 1096 c 1103 g 437 t
ORIGIN
1 aagcttttgg aggggaagcc catctacatg taccccacgt ccattgaggc ggccaaggcc
61 atcgtggcca tggtgggagg tgcggcgtga tcctggtgaa ccgcgagacc cgcgtcctgg
121 tccagggcat caccggccgg gaggggcagt tccacaccaa gcagatgctg gactacggca
181 ccaagatcgt cgccggggtc accccgggca aagggggaac ggaggtccta ggggtccccg
241 tctacgacac ggtgaaggag gcggtggccc accacgaggt ggacgcctcc atcatcttcg
301 tgcccgcccc ggccgcggcg gacgccgccc tggaagcggc ccacgccggg atccccctca
361 tcgtcctcat caccgagggc atccccaccc tggacatggt gcgggcggtg gaggagatca
421 aggccctggg aagccgcctc atcgggggga actgccccgg gatcatcagc gccgaggaga
481 ccaagatcgg gatcatgccc ggccacgtct tcaagcgggg ccgggtgggg atcatcagcc
541 gctccggcac cctcacctac gaggccgcag ccgccctttc ccaggcgggg ctcggcacca
601 ccaccacggt ggggatcggg ggcgaccccg tcatcggcac caccttcaag gacctcctcc
661 ccctcttcaa cgaggacccg gagacggagg ccgtggtcct catcggggag atcggcggct
721 ccgacgagga ggaggcggcg gcttgggtga aggaccacat gaagaagccg gtggtgggct
781 tcatcggagg ccgctccgcc cccaagggca agcgcatggg ccacgccggg gccatcatca
841 tgggcaacgt gggcaccccg gagtccaagc tccgggcctt cgccgaggcg ggcatccccg
901 tggccgacac catcgacgag atcgtggagc tggtcaagaa ggccctgggc taaaaaggag
961 gtggacggtg aaggcacccg tacgcgtggc ggttaccgga gccgcgggac agatcggcta
1021 cagcctcctc ttccgcatcg ccgcggggga gatgctgggc aaggaccagc cggtgatcct
1081 ccagcttttg gagatccccc aggccatgaa ggccctggag ggcgtggtca tggagctgga
1141 ggactgcgcc ttccccctgc ttgcgggcct cgaggccacc gacgacccca aggtggcctt
1201 caaggacgcc gactacgccc tcctggtggg ggcggccccc cgcaaggcgg ggatggagcg
1261 ccgggacctt ttgcaggtaa acggcaagat cttcaccgag cagggccggg ccctggccga
1321 ggtggccaag aaggacgtca aggtgctggt ggtgggcaac cccgccaaca ccaacgccct
1381 catcgcctac aagaacgccc ccggcctcaa cccccggaac ttcaccgcca tgacccggct
1441 ggaccacaac cgggccaagg cccagctcgc caagaagacc gggacgggcg tggaccgcat
1501 ccgccgcatg acggtgtggg gcaaccactc ctccaccatg ttccccgacc tcttccacgc
1561 cgaggtggac ggcaggcccg ccctggagct cgtggacatg gagtggtacg agaaggtctt
1621 catccccacc gtggcccagc ggggggcggc catcatccag gcccgggggg ccagcagcgc
1681 cgccagcgcc gcgaacgccg ccatagagca catccgcgac tgggccctgg gcaccccgga
1741 gggggactgg gtttccatgg ccgtcccctc ccaaggggag tacggcatcc ccgagggcat
1801 cgtctactcc ttcccggtga cggccaagga cggggcgtac cgggtcgtgg agggcctgga
1861 gatcaacgag ttcgcccgca agcgcatgga gatcacggcc caggaacttc tggacgagat
1921 ggagcaggtg aaggccctgg gcctcatctg agaggctggc ctcagagccc ccaccgcgcc
1981 ctgcggtggg ggctttacac caccccatgc tggcttgcgc cagcatgggg gccccggcaa
2041 aagctccctg gggagcttcc ccgggcccgt cgggcgaagg caaccggaag ggtattagcc
2101 tttgggccag gcctcgaggg cccaagggta gaccacccag gccgccgtct cctcggcgta
2161 gaagtcgggg cggtccggca cccggttccg ccccggcttg aagtggaggg tggccaccac
2221 aggcaccccg cccgcccggc gcacccgggc cttcacggca aaggccgtgc gccccgagtc
2281 ccaaacgtcg tccaccacca ggacccgctt gccgaaaagg aggacatcgg ggggaaactg
2341 caggaatacc ggctcgggca aagcctcctc cccctcgtag aacatgacgg cggcggtgag
2401 gatgttccgc gcgccgaggg cctgggccag aagggcggtg gggatgaggc ctccccgcgc
2461 cacccccagg acgaggtcaa aaggctcccc ggaaagggcc tccgccaggc ggcggaccag
2521 ccggaggagg tcctcccagg aaaggtggag gcgctccatc aggccttgtc cagctcaaag
2581 gcctggtgga cggccctaag cgccgcctcg gcgtactcgg cggggatgat cacggagatg
2641 cgcacctcgc tggtggcgat catctcaatg ttggccccgg tggaggccac cgcctggaac
2701 atcttggcgg ggatctccgg agtggaggcg aggcccacgc ccacgatgga gaccttggcg
2761 atgtccggcc tcaggatggc ctcgcccccg atctcggcga ggacgggctc caaagcctcc
2821 agggcctcct gggcgaagtc cttcttcacc gtgaaggcca tctgctgcct cgaggggtcg
2881 tggccgggac cccctggatg atcatgtcca cggcgatgcc ccgctcggcc aaagcctgga
2941 agaccttggc cgcgatgccc ggctggtccg ggatgccgat aagcccgatc tgggcgtggt
3001 ccaggtccaa ggccacgccc gtcaccgcct tgtccatctc catggcgacc tccttcacca
3061 gggtaccggg gttgtaggag aagctagagc gcacgtggag gaccaccccg tagcgcttgg
3121 cgtagtagac cgcccggggg tggaggaccc tggcccccaa agcggccatc tccagcatct
3181 ggtcgtagcc gatgacggaa agctt
//