GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS TFEMOBLS 2797 bp ds-DNA BCT 27-MAY-1991
DEFINITION Thiobacillus ferrooxidans plasmid pTF1 mobilization genes mobL and
mobS
ACCESSION X52699
KEYWORDS mobL gene; mobS gene; mobilization protein; plasmid.
SOURCE Thiobacillus ferrooxidans DNA.
ORGANISM Thiobacillus ferrooxidans
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Colorless sulfur bacteria.
REFERENCE 1 (bases 1 to 2797)
AUTHORS Lau,P.C.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2797)
AUTHORS Drolet,M., Zanga,P. and Lau,P.C.
TITLE The mobilization and origin of transfer regions of a Thiobacillus
ferrooxidans plasmid: relatedness to plasmids RSF1010 and pSC101
JOURNAL Mol. Microbiol. 4, 1381-1391 (1990)
STANDARD full automatic
COMMENT *source: strain=ATCC33020 **map: map position=3.867 to 6.657 +
0.006 units
From EMBL entry TFMOBLS; dated 02-NOV-1990.
FEATURES Location/Qualifiers
CDS complement(304..615)
/note="ORF7"
/codon_start=615
CDS complement(621..914)
/note="mobS protein (ORF6)"
/codon_start=914
misc_feature 1032..1032
/note="relaxation nick site"
misc_feature 1039..1062
/note="hairpin at oriT"
CDS 1112..2245
/note="mobL protein (ORF2)"
/codon_start=1112
CDS complement(1130..1576)
/note="ORF5"
/codon_start=1576
CDS 1563..1787
/note="ORF3"
/codon_start=1563
CDS 1869..2192
/note="ORF4"
/codon_start=1869
CDS complement(2382..>2797)
/note="ORF11 (2797 is 3rd base in codon)"
/codon_start=2797
BASE COUNT 611 a 749 c 865 g 572 t
ORIGIN
1 agatccaggc tctggaggcc aggtttaccg aactgaccgg gaccatcgag gagcaacggc
61 aggagatcga gcgacggcgg caagaggcca cggaagcccg ggagaccgcc gccgagttgc
121 gcgggcaggt cgaggcattg caacgggtga tctcggaatg gcaaccgcgg aaaccttccg
181 gggccgggag aggcgagaaa taatcacatc accccggggg gatacaccac tctggccgag
241 agaatcgctc tgagggcaga tttggggccc tggcggggtc gtttctgagg gtgaatgcgt
301 ttaaatttct atgccgaggc ttcgctcatg gctgatgtag atggactggc cgatgtcatg
361 ccaatgctgc ctcttttttt ccaggctgat gcccagatcg agtccgtctg cgaactctgc
421 ggcctcttca tccgtctgga gcatgagcat taccaatccc atgatttctt ttctcagttt
481 ggccgacagc tcattcaaga cgatggtctg gacctctttg gaagagaact tggtattgat
541 ggtcgatcct acgagcacat ctaaaccagc cagaacactc agcaactgtt ccctcacttc
601 ctgcacgttt tccatcacta tttttcctct ggtatgggca attgaaagag ggcgcggtca
661 tcggcccgcg tcaggtattt atccagtcga tccataaatc gttgtctggt agattcatcg
721 cgttccatca tgtccagcac caatgcccct accaggatct tccggcgcgt atcatccgag
781 cgttggccct tgagcaagga ttgcagtttg cgggcctcga ccatatcccg tttggccttg
841 agttgcttga cctgtttagc ggcctcctgt gcgcgttttt cagcggcggc tattttttcc
901 tcgaaagacg ccatgagtac ccctgtctgt tgatgtttat ggggtgattc tagcgtaagc
961 tggacggatg agcgataggc agcatagcgt ggttatatgc tggacatcat cccgacgctg
1021 gaaagcaagg gcgcacttag agtaatctct tcgagattac ccgtgcggtc aagggctacg
1081 cccctgacaa ccccaaagga gggaagcgcc catggcgatc taccacctat ccgcgaagcc
1141 gatcagtcgc agcgccggac gttccgcgac tggagcggcg gcctatcgcg caggtgtgga
1201 gatcaccgac gagcgtaccg gcctggtgca tgactacacc cgcaagggtg gcgttctgca
1261 ctccgaactc atcctgcctg gtggtggaac cgccgaccga gccgagttct ggaacggcgt
1321 cgaagcgcac cacaagcgcg gcgatgccgt cctcgtccgg gagatagaaa tctccctgcc
1381 gaccgaactc acggcagagc agcggaaggc gctcgccgta ggctacgccc gtgcgcttgc
1441 cgaccgttac ggtgtagcgg cagatgttgc cctgcacgcg ccgcgcaccg tgaccgaccg
1501 ggagcttgaa aagcaccccg accagtacca cgaaaccgac cccaagacag gtcgccgtca
1561 taatggcaac tggcacgcgc acatcctgct atcggcgtgc cacgtccagc ctgatggcac
1621 gcttggcaag aaggccgtcg agcttgaccc gattcactgc cagcgagcga agatcgagaa
1681 cctggtggac cgggagcggg tgcgctgggg tgagctggcg aacgcagcgc ttgagcacca
1741 cggtcacgcg gcccgcatcg accaccgcag ccacgctaag cgcgggatag aagccgaacc
1801 gactcggcac ctcggtccag cggcggcagg gattgagcga aggacgggcg aggaaagccg
1861 caggcggcat gatttcgagc gggaagccat ggaacggctg gcccgtgccc aagcgcaagg
1921 tgtgttggag cgagaatcgc ggggactgga gcgaagtatc tttgacctgt caggcgatat
1981 gcgggctgcg ttccaggagc gggacaggct cagggaacag caggaacggg ctgaaagaga
2041 gcggcgggag caggagcggg ccagggaagc ccagaggcaa caggaaaagc agcaggcgga
2101 taaggccgcg tccgaccagc gagaagccga gaggatactg gcgcagctcc tggagaaggc
2161 cccggtcgtg gatagaaaga cggaaaaggc catagatggg ctatttcacg acatagcgcg
2221 gagtcggtca cggggacgcg ggcggtgatg tggtatcgcg tagccgtcac agtggcaggt
2281 ggagcgcggc aggggtttgg cggacgatga aaggtggatg gtttactgga cagaatcagg
2341 gccgaaaaag gccccggaag tgtccagata ccaggtgttc aggaaaggtt ggtttttaca
2401 ccgtcaggct gtttagtcac ggcgtttgca agtaaggcat aaaaaacgct tagcataagg
2461 ccaacgagcg cacctagcac gatgaaaacg gctttgcctg gtccaactgg ttcaatcgaa
2521 ggtgatgatg gacgaacaat atgcgtgctt tgcaatgcct ttattgatgc cttatccagt
2581 tctatctgcg cagagtttgc tgcaatttgt gcattaagat tggtttcttg tctctttagt
2641 ttgttcatct ggttaagcaa ccctatttgt tcttttggca aggctacaga cctctccatt
2701 tctaagttga acaactgttg gcgttcctga gccacctggt tgttctggat catcatggtc
2761 aatgctgtgg cattatcatg aacatttttt gaagctt
//