GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS VITTHB 648 bp ds-DNA BCT 27-MAY-1991
DEFINITION Vitreoscilla sp. hemoglobin gene
ACCESSION X13516
KEYWORDS hemoglobin.
SOURCE Vitreoscilla sp. DNA.
ORGANISM Vitreoscilla sp.
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Nonphotosynthetic, nonfruiting gliding bacteria; Cytophagales;
Beggiatoaceae.
REFERENCE 1 (bases 1 to 648)
AUTHORS Khosla,C. and Bailey,J.E.
TITLE The Vitreoscilla hemoglobin gene: Molecular cloning, nucleotide
sequence and genetic expression in Escherichia coli
JOURNAL Mol. Gen. Genet. 214, 158-161 (1988)
STANDARD full automatic
COMMENT SWISS-PROT; P04252; BAHG$VITSP.
*source: library=pUC19; clone=pRED2 and pRED4;
From EMBL entry VITHB; dated 03-FEB-1990.
FEATURES Location/Qualifiers
promoter 14..20
/note="-35 region"
promoter 32..38
/note="-10 region"
RBS 89..92
/note="pot. ribosome binding site"
CDS 101..538
/note="hemoglobin (AA 1 - 146)"
/codon_start=101
BASE COUNT 192 a 123 c 155 g 178 t
ORIGIN
1 tgtggattaa gttttaagag gcaataaaga ttataataag tgctgctaca ccatactgat
61 gtatggcaaa accataataa tgaacttaag gaagaccctc atgttagacc agcaaaccat
121 taacatcatc aaagccactg ttcctgtatt gaaggagcat ggcgttacca ttaccacgac
181 tttttataaa aacttgtttg ccaaacaccc tgaagtacgt cctttgtttg atatgggtcg
241 ccaagaatct ttggagcagc ctaaggcttt ggcgatgacg gtattggcgg cagcgcaaaa
301 cattgaaaat ttgccagcta ttttgcctgc ggtcaaaaaa attgcagtca aacattgtca
361 agcaggcgtg gcagcagcgc attatccgat tgtcggtcaa gaattgttgg gtgcgattaa
421 agaagtattg ggcgatgccg caaccgatga cattttggac gcgtggggca aggcttatgg
481 cgtgattgca gatgtgttta ttcaagtgga agcagatttg tacgctcaag cggttgaata
541 aagtttcagg ccgctttcag gacataaaaa acgcaccata aggtggtctt tttacgtctg
601 atatttacac agcagtttgg ctgttgccaa aacttgggac aaatattg
//