GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS HUMIGK12 2352 bp ds-DNA PRI 27-MAY-1991
DEFINITION Human germ line pseudogene for immunoglobulin kappa light chain
leader peptide and variable region (subgroup V kappa I)
ACCESSION Z00010 X00903
KEYWORDS germline; immunoglobulin; immunoglobulin light chain; pseudogene;
signal peptide; variable region.
SOURCE Homo sapiens DNA.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (sites)
AUTHORS Zachau,H.G.
JOURNAL Unpublished (1984)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 2352)
AUTHORS Pech,M., Jaenichen,H.-R., Pohlenz,H.-D., Neumaier,P.S.,
Klobeck,H.-G. and Zachau,H.G.
TITLE Organization and evolution of a gene cluster for human
immunoglobulin variable regions of the kappa type
JOURNAL J. Mol. Biol. 176, 189-204 (1984)
STANDARD full automatic
REFERENCE 3 (bases 1491 to 2192)
AUTHORS Straubinger,B., Pech,M., Muehlebach,K., Jaenichen,H.R., Bauer,H.G.
and Zachau,H.G.
TITLE Molecular footprints of human immunoglobulin gene evolution: a new
sequence family
JOURNAL Nucleic Acids Res. 12, 5265-5275 (1984)
STANDARD full automatic
COMMENT From EMBL entry HSIGK12; dated 06-JUL-1989.
FEATURES Location/Qualifiers
unsure 263..263
/note="may be C or T"
misc_feature 299..313
/note="put. regulatory element pd"
misc_feature 330..339
/note="put. regulatory element dc"
promoter 372..379
/note="TATA-box"
CDS 427..481
/note="first exon (leader peptide) (481 is 1st base in
codon)"
/codon_start=427
intron 482..607
/note="first intron"
CDS 608..903
/note="second exon (rest of L and variable region)"
/codon_start=608
misc_feature 721..723
/note="stop codon (pseudogene)"
misc_feature 906..912
/note="heptanucleotide box, not canonical"
misc_feature 1581..2064
/note="L-sequence region"
unsure 2257..2257
/note="not unambiguously determined"
unsure 2300..2300
/note="not unambiguously determined"
BASE COUNT 685 a 497 c 510 g 657 t 3 others
ORIGIN
1 cgggatcata tgagagtctt ttggagacct gataatcata ccgtctaaca ttttattata
61 tatttcctac aaacaagaat attctcctaa ataatcccca tacaccaatg aaatacatta
121 ctccatcaac tcctgaggaa tatttcaaat tgtcaaaaaa aacctaaaaa atgtctctca
181 taataaaata gttcccagta gaaacacatt ctctggagac aaatttgtgc taccctggtc
241 ttacctggga cacctgggga caytgaactg gtgctgagtt actgagatga gccagccctg
301 cagctgtgcc cagcctgccc atcctctgct catttgcata ttcccagaac acaacctcct
361 gccctgaaga cttcttaata ggctggtcac acttcttgca ggagtcagac ccactcagga
421 cacagcatgg acatgagggt ccccgctcag ctcctggggc ttctgctgct ctggctccca
481 ggtaaggaag gagaacacta gcagtttact cagcccaggg ggctcagtac agcctggcta
541 ttcagggaaa ttctcttact acatgattaa ttgtgtggac catttgtgtt tatgcttcca
601 atctcaggtg ccagatgtgc catccagttg acccagtctc catcctccct gtctgcatct
661 gtaggagaca gagtcaccat cacttgccgg gcaagtcagg gcattagcag tgctttagcc
721 tgatatcagc agaaaccagg gaaagctcct aagctcctga tctatgatgc ctccagtttg
781 gaaagtgggg tcccatcaag gttcagcggc agtggatctg ggacagattt cactctcacc
841 atcagcagcc tgcagcctga agattttgca acttattact gtcaacagtt taataattac
901 cctcacatag tgttacaaac ccgaacataa acccccaggg aagcagatgt gtgagactgg
961 gccgccccag ctgcttctcc tgatgcctcc attggctgag agtgttcctc agatgcagcc
1021 acactctgat ggtgttggta gaggaggata tgagatcacc tctgcatccc aatttctttt
1081 tcttttctca gccccagctg cacagacatt acaatgcctc tgctgattta ataaagatag
1141 agatcatgac acctgaagag tctagtttat ggctttggtt agaattcata taacagagaa
1201 gaagccatta tagatattct aagcaggaat agtcttaata gatagaatta gagtctaaag
1261 tattgaagtc taaataaaat gtacagataa atttaatgtt ttatttgcta agaaattttt
1321 gccaaatggg gcatacagga aaactcaatg gtcttcaata tgttggaaga gcaaagagtt
1381 ttattaaaag ggaaattatt acctattgtt ctttgagaaa ttttgttggc tgtagtaagg
1441 gttgggagct ggcaagctca gactggtaag cagtggtggt caaactgaat cctagaatta
1501 tattaagtta tctcagaagt tgtgggtaaa tttgctttca ggttacaata agccaaagca
1561 gtgaagcttg cagagaattt tgttactgaa atgccaggga ttcagtatag atcctgcggc
1621 tcaccacaca gaaagccaat cactaagaca acaagtgttg tcaaagaaca ggctttaatc
1681 aggtgctgca gccgaggaga cgggacacca ttctcaaatg tatctccctg acaaaataaa
1741 ttaggcgttt atatagcagg gaagaaatgt ggaaaacagg aattagagag gggtaaggaa
1801 gataatttag tcaacaggaa gctggaggtc agttaggcaa tcataatggg tgaagggtct
1861 gatgtctcac tgtccccatt cagtgatata taactttcag ctccttgata gtatctagag
1921 gcctgatggt tggtttcctg aaaaaagaac tcagattaac aaatgtaact accttgagtt
1981 ttaagactgg gggagtcagt ttctatgttt attcaaaaaa tcataaacat tagttccatg
2041 ggataatagg gtctatttca attgcattct tcagacaata ttttgcaccc tgagtggttt
2101 tccctcctgg tttcttggct ctgttgggta tgtcaagaat gaccgaattc tatgattaac
2161 ttttacacta caacctttca aagccaagga tatagtagtc aggcaggttg acattagaag
2221 caggattctc tggtactccc tcagaaaata gaatgcntct cgccactgaa gtatgggcta
2281 tctaaccatg tggtcctcan tcctgtctga aagcttaagg gtggggttgc agctgctctc
2341 agcttcctat ag
//