GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS HUMIGK19 1863 bp ds-DNA PRI 27-MAY-1991
DEFINITION Human rearranged DNA for kappa immunoglobulin subgroup V kappa II
ACCESSION Z00020
KEYWORDS immunoglobulin; joining region; kappa-immunoglobulin;
rearranged DNA; signal peptide; variable region.
SOURCE Homo sapiens DNA.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1654 to 1752)
AUTHORS Hieter,P.A., Max,E.E., Seidman,J.G., Maizel,J.V. and Leder,P.
TITLE Cloned human and mouse kappa-immunoglobulin constant and J region
genes conserve homology in functional segments
JOURNAL Cell 22, 197-207 (1980)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1863)
AUTHORS Zachau,H.G.
JOURNAL Unpublished (1985)
STANDARD full automatic
REFERENCE 3 (bases 374 to 1863)
AUTHORS Klobeck,H.-G., Meindl,A., Combriato,G., Solomon,A. and Zachau,H.G.
TITLE Human immunoglobulin kappa light chain genes of subgroups II and
III
JOURNAL Nucleic Acids Res. 13, 6499-6513 (1985)
STANDARD full automatic
COMMENT SWISS-PROT; P06310; KV2F$HUMAN.
From EMBL entry HSIGK19; dated 06-JUL-1989.
FEATURES Location/Qualifiers
misc_feature 777..788
/note="put. regulatory element pd"
misc_feature 790..799
/note="put. regulatory element dc"
promoter 837..844
/note="TATA-box"
CDS 898..946
/note="signal peptide (exon 1) (aa -20 to -5) (573 is 1st
base in codon)"
/codon_start=898
intron 947..1372
/note="intron I"
CDS 1373..1383
/note="signal peptide (exon 2) (aa -4 to -1) (1000 is 2nd
base in codon)"
/codon_start=1373
CDS 1384..1747
/note="variable region (exon 2)"
/codon_start=1384
CDS 1453..1500
/note="CDR1"
/codon_start=1453
CDS 1546..1566
/note="CDR2"
/codon_start=1546
CDS 1663..1689
/note="CDR3"
/codon_start=1663
conflict 1683..1683
/note="C is A in [2]"
/citation=[1]
CDS 1684..1720
/note="J1 gene segment"
/codon_start=1684
conflict 1701..1701
/note="G is A in [2]"
/citation=[1]
intron 1721..1863
/note="intron II"
BASE COUNT 562 a 366 c 411 g 524 t
ORIGIN
1 ggattcaatt atccagtgaa agtatgcatt caaaataaaa gtaaagtaaa aacatttctt
61 gataaacaaa aatgaagaga aataattact taaagacatg ctgtataaca atttatctag
121 gaaattcttc agattcacaa aaaatgacag cagacagtaa cttgaatctg taaggaggaa
181 ggaaggcctc aaaagtacta agggaacaaa aaaagagcta gaccaaacca acatgtttac
241 ttccgtttat atgaggtttc gtatagggaa agccaatcta tttggacaga tgtcagaatg
301 ccagtttcct tgattgggat gggaaacagc tgtttcctga aaggtaggta catgagggaa
361 gaatctggag attctgcagt attctacagt ttaatctcgg tggagaatat atgtaaaact
421 ttattcggtt gcacttttta acatttctgt cttttacttt gtgtgtgttt tatttaaatt
481 tttaaaaaat tgaaagggcc aaatctgaac tcttttaaac aaaaatgaac aaaaacataa
541 gaattagtaa atatttgtgg aaacatggcc ttattaacaa gaagtataaa atgtgcctgg
601 gagagtacca tgaaacaaga aatctgttag ggaagacaga aggaaatact taaatttctc
661 caacatagac agcatagatt ttatgcctat tcgtttccct ccaaacagag aagatattta
721 agtcattttg ctcacaagag aggctcctac cctccccttg gctctttcca ccccactgca
781 cccaccaggt gatttgcata ttatccctta gtgaagactt tccttgtgag tctgagataa
841 aagctcagct ctacccttgc cttgactgat caggactcct cagttcacct tctcacaatg
901 aggctccctg ctcagctcct ggggctgcta atgctctggg tcccaggtaa gggtagaagg
961 gagatgaggg aggagaatgg catggaacgg tgagttctgg ggccccactg cctctaacaa
1021 cagtgatctc tgggggtctc actacactcc tatgtgtgtt cctttcctgt attggacatg
1081 cacatgttgt cctccagagt ggggcatgtg atgatcagat ctgtgagagt gaggaagatt
1141 caagcagaaa ctaaggatct gtgctctggg gaagactgac acagaaaggg gatggtgtgg
1201 ggtcttctgg agaccctttt gagccttgga tccttgagtt ccattttgaa actgtgtatt
1261 tttgaaatat gaacaaatac atatatagcc tgaaataaac aacaaatcaa aatttatgaa
1321 aattacacat aaactttata cataaccttg ctcttctttc tatttatttc aggatccagt
1381 ggggatgttg tgatgactca gtctccactc tccctgcccg tcacccttgg acagccggcc
1441 tccatctcct gcaggtctag tcaaagcctc gtatacagtg atggaaacac ctacttgaat
1501 tggtttcagc agaggccagg ccaatctcca aggcgcctaa tttataaggt ttctaaccgg
1561 gactctgggg tcccagacag attcagcggc agtgggtcag gcactgattt cacactgaaa
1621 atcagcaggg tggaggctga ggatgttggg gtttattact gcatgcaagg tacacactgg
1681 tcctggacgt tcggccaagg gaccaaggtg gaaatcaaac gtgagtagaa tttaaacttt
1741 gcttcctcag ttgtctgtgt cttctgttcc ctgtgtctat gaagtgatct ataaggtgac
1801 tctgcaatca gcctctgata tccttcaggg aaaagataaa gataagtctg tagtcaaact
1861 cga
//