GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS HUMIGK4 911 bp ds-DNA PRI 27-MAY-1991
DEFINITION Human germline pseudogene for leader peptide and variable region of
a kappa immunoglobulin (subgroup V kappa I)
ACCESSION Z00002 X00746
KEYWORDS germline; immunoglobulin; kappa-immunoglobulin; pseudogene;
variable region.
SOURCE Homo sapiens DNA.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 911)
AUTHORS Jaenichen,H.R.
JOURNAL Unpublished (1984)
STANDARD full automatic
REFERENCE 2 (bases 1 to 909)
AUTHORS Jaenichen,H.R., Pech,M., Lindenmaier,W., Wildgruber,N. and
Zachau,H.G.
TITLE Composite human V kappa genes and a model of their evolution
JOURNAL Nucleic Acids Res. 12, 5249-5263 (1984)
STANDARD full automatic
COMMENT The sequence is designated V2 in refs. [1] and [2]; the start and
stop positions of the leader peptide were defined according to
sequence comparisons; several mutations render the leader gene
segment inactive ; the variable region gene segment is inactivated
by a stop codon at the amino acid position 71.
From EMBL entry HSIGK4; dated 13-JUN-1985.
FEATURES Location/Qualifiers
unsure 82..82
/note="maybe A or C"
misc_feature 91..105
/note="put. regulatory element pd"
misc_feature 123..132
/note="put. regulatory element dc"
promoter 165..171
/note="TATA-box"
CDS 219..273
/note="first exon (leader peptide) (273 is 1st base in
codon)"
/codon_start=219
intron 274..397
/note="first intron"
CDS 398..693
/note="second exon (rest of L and variable region) (398 is
2nd base in codon)"
/codon_start=398
misc_feature 696..702
/note="heptanucleotide box"
BASE COUNT 215 a 247 c 223 g 225 t 1 others
ORIGIN
1 ctttcaaggt aaattggtgc tgccctggtc tgacctggga ctctagggga tactgcgtcc
61 ctgtgctgag ttactgagat gngccagccc tgcagctgtg ctcagcctgc cccatcccct
121 gctgatttgc ctgttcctag agcacagccc cctgccctga agacttttta taggctggtc
181 acacccggtg caggagtcag ccccagtcag gacacagcac ggatgcaagg tcccccactc
241 agctcctggg gctcctggtg ctctggttgc caggtaagga aggagaacac taggattata
301 ctcggtcagt gtgctcagta ctgtctggaa cctcagggaa gtcctctgat aacatgatta
361 attgcaagaa tatttgtttt tatgtttcca actgcaggtg ccagatgtga catccagatg
421 acccagtctc catcctccct gtctgcatct gtaggagaca gagtcaccat cacttgccgg
481 gcgagtcagg gcattagcaa taatttaaat tggtatcagc agaaaccagg gaaaactcct
541 aagctcctga tctatgctgc atccagtctg caaagtggga ttccctctca gttcagtgac
601 agtggatctg ggacagatta gactctcacc atcagcagcc tgcagcctga agattttaca
661 acttattact gtcaacagag ttacagtacc cctcccacag tgttgcaagt cataacataa
721 accccaagga agcagatgtg tgaggctggg ctgccccaat gctccttctg gtgcctctat
781 ctgctgaggg aagttctcaa actcagtcag gcttggaaag tcattgggag attttcctag
841 aggaggccag ggaggttcct ctgaacccta agcctctttc gccctcatcc ccagcagaca
901 agatgtgaca a
//